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Citations for Click Chemistry Tools :
1 - 50 of 78 citations for 7 Benzyloxy 10 11 dihydrodibenzo b f 1 4 thiazepin 11 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (200 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (11 μL, 110 μmol, Click Chemistry Tools) followed by DIEA (38 μL ...
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bioRxiv - Bioengineering 2023Quote: ... Azide-labelled pHPMA-b-pBMA or pMAA-b-pBMA polymer was reacted with AFDye647-DBCO (Click Chemistry Tools) and then formulated into micelles ...
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bioRxiv - Biochemistry 2020Quote: ... 1.6 mM BTTAA ligand (2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)−1 H-1,2,3-triazol-1-yl)acetic acid) (Click Chemistry Tools Scottsdale, Az), and 5 mM sodium ascorbate ...
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bioRxiv - Molecular Biology 2019Quote: Biotin was attached to metabolically labeled RNAs in a 10–20 μl reaction with 4 mM biotin disulfide azide (Click Chemistry Tools), 5 mM CuSO4 (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: The Click reaction was then performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy5 picolyl azide (Click Chemistry Tools 1171-1)) for 1 hour at 37°C in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... in situ click reactions were performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy3 picolyl azide (Click Chemistry Tools 1178-1)) for 1 hour at 37°C in the dark ...
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bioRxiv - Genomics 2022Quote: ... 10 µL of 10 mM DBCO-NHS (Click Chemistry Tools cat# A134-10) was then added to the antibodies and allowed to rotate at room temperature for one hour 26 ...
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bioRxiv - Bioengineering 2021Quote: ... 2-(4-((bis((1-(tert-butyl)-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)acetic acid (BTTAA, Click Chemistry Tools > 95%), Click-IT™ Fucose Alkyne (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... they were reacted with 10:1 Methyltetrazine-sulfo-N-Hydroxysuccinimide ester (Met-NHS) (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Bioengineering 2020Quote: ... The reduced C-terminal cysteine (1 eq.) was reacted with sulfo DBCO-maleimide crosslinker (4 eq.) (Click Chemistry Tools, AZ, USA) in PBS (pH 6.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were then washed with ice-cold D-PBS 4 times and incubated in D-PBS supplemented with 1 μM Cy5.5-azide (Click Chemistry Tools), 1 mM TCEP (adjusted to pH 7.0) ...
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bioRxiv - Molecular Biology 2022Quote: Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
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bioRxiv - Developmental Biology 2019Quote: ... 4 mM disulfide biotin azide (Click Chemistry Tools, Scottsdale, AZ) was added to a 10 μL reaction containing RNA ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μL of 1 mg/mL antibodies were reacted with 1 μL of 10 mM UV-cleavable azido-NHS ester (30 equivalents to antibodies; Click Chemistry Tools) for 2 h ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μM AFDye 488 Azide (Click Chemistry Tools), and 100 mM ascorbic acid in the dark at room temperature for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... NHS-PEG-TCO ester (A137-10, Click Chemistry Tools), poly(ethylene glycol ...
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bioRxiv - Genomics 2022Quote: 100 µl of 100 µM oligo barcode (IDT) in PBS and 10 µl of 10 mM azide-NHS (Click Chemistry Tools cat# 1251-5) in DMSO were mixed at room temperature for two hours ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1.5 µl of 10 mM Azide-PEG4-Biotin (Click Chemistry Tools), the click reaction was conducted for 60 min at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... Cy5.5-NHS ester (purchased from Click Chemistry Tools, for Lys-cy5.5 conjugate 10), or DBCO–Cy5.5 (purchased from Click Chemistry Tools ...
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bioRxiv - Molecular Biology 2023Quote: ... were incubated with 10 mM biotin azide (probe condition; Click Chemistry Tools 1265) or 10 mM desthio-biotin (no probe condition ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS and incubated in 10 μM AZDye 488 DBCO (Click Chemistry Tools) for 30 min at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... gels were soaked in 10 µM Cy5-N3 (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to block any unreacted BCN groups ...
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bioRxiv - Bioengineering 2024Quote: ... gels were soaked in 10 µM Cy5-N3 (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to label the SPAAC network and then washed 3 x 1 hr in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 44 µL of a 1:1 mixture of BTTAA (Click Chemistry Tools, 77.4 mg mL-1 PBS) and copper sulfate pentahydrate (7.4 mg mL-1 water) ...
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bioRxiv - Bioengineering 2022Quote: ... 50 μL of antibodies were reacted with 10 mM photocleavable azido-NHS ester (Click Chemistry Tools) at 1 ...
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bioRxiv - Synthetic Biology 2020Quote: ... SpoIVA was labelled with 10-fold molar excess of trans-cyclooctene-PEG3-Maleimide (Click Chemistry Tools) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... and then resuspended to OD600 of 0.6 in 1 mL PBS + 1 μM AZDye 488 DBCO (Click chemistry tools, cat# 1278-1). Cells were covered with aluminum foil and rocked for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... RPAAZP (∼ 4 µM in 5 mL of storage buffer) was mixed with 1.5-fold molar excess DBCO-MB543 (Click Chemistry Tools Inc., AZ) or DBCO-Cy3 ...
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bioRxiv - Bioengineering 2023Quote: 7-(Diethylamino)-4- (hydroxymethyl)coumarin (DEAC-OH) was previously synthesized in-house (Method S3) and conjugated to 2-azidoacetic acid (Click Chemistry tools, 1081), 3-azidopropionic acid (Synthonix ...
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bioRxiv - Biochemistry 2023Quote: ... 330 μL TCEP (14.0 mg/mL in H2O) and 160 μL of either heavy or light isoDTB tags (4 mg in DMSO, Click Chemistry Tools, 1565). The samples were then treated with 120 μL of the heavy (DMSO treated ...
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bioRxiv - Biochemistry 2024Quote: ... 165 µL TCEP (14.0 mg/mL in H2O) and 160 µL of either heavy or light isoDTB tags (4 mg in DMSO, Click Chemistry Tools, 1565). The samples were then treated with 120 µL of the heavy (DMSO treated ...
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bioRxiv - Immunology 2020Quote: ... 8 μL of 100 mM TCO-PEG4-NHS in DMSO (10 mM final; Click Chemistry Tools, A137), pH 8.5 was rotated at room temperature for 15 min ...
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bioRxiv - Biochemistry 2020Quote: ... is combined with DBCO-Peg5-NHS Ester (linker; 10 mM in DMSO; Click Chemistry Tools Cat: 1378531-80-6) in 60 molar excess (50 mg of Rabbit IgG and 4.6 mg of linker) ...
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bioRxiv - Neuroscience 2024Quote: ... were submerged in dimethyl sulfoxide (DMSO) with 10 mM dibenzocyclooctyne-PEG-amine (DBCO-PEG4-amine; Click Chemistry Tools, A103P) overnight at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... and dibenzocyclooctyne-sulfo-N-hydroxysuccinimide ester (DBCO-Sulfo-NHS ester, Click Chemistry Tools, prepared as 10 mM stock in DMSO) at a 2500:1:1000 molar ratio of dye:particle:DBCO ...
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bioRxiv - Bioengineering 2024Quote: ... 40 µL of 10 mM Cy7-NHS Ester (#1115) or AZDye 488-NHS Ester (#1338) (both from Click Chemistry Tools) in DMSO was added to the solution dropwise in dark at 4 °C ...
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bioRxiv - Bioengineering 2020Quote: ... cytokines were reacted with a commercial 2-nitrobenzyl linker displaying both NHS ester and DBCO substituents (1160-10, Click Chemistry Tools) followed by addition of poly(ethylene glycol ...
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bioRxiv - Biochemistry 2019Quote: ... artificial amino acid-containing base was incubated at room temperature with 150 µM 5,5′-dithiobis-2-nitrobenzoic acid for 10 minutes before chilling on ice and adding 300 µM DBCO-Cy3 (Click Chemistry Tools) and incubating at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was terminated by quenching with a 10 mM Tris-HCl [pH 8.0] buffer supplemented with 50 µM methyltetrazine-DBCO (Click Chemistry Tools). The methanol-preserved cells conjugated with ClickTags were rehydrated following Supplementary Protocol 3.
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bioRxiv - Cell Biology 2024Quote: ... CalFluor 647 Azide(Click chemistry tools, 1272-1); Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric ...
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bioRxiv - Biochemistry 2022Quote: HEK293A cells with or without TEAD overexpression was pretreated with DMSO or TM2 in medium with 10% dialyzed fetal bovine serum (DFBS) for 8 hand labeled by Alkynyl Palmitic acid (1165, Click Chemistry Tools) for another 16 h ...
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bioRxiv - Biochemistry 2023Quote: 10 μM of RPA4AZP (0.5 mL) was mixed with 2X molar excess of the dibenzocyclooctyne-MB543 (DBCO-MB543; Click Chemistry Tools Inc.) dye and incubated at 4 °C for 1.5 h with gentle rocking in dark ...
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bioRxiv - Biochemistry 2023Quote: ... the substrate was diluted to 100 μM and incubated for 10 minutes at room temperature with 200 μM SulfoCy5 (for stopped-flow measurements, Click Chemistry Tools) or LD555 (for smFRET measurements) ...
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bioRxiv - Biochemistry 2023Quote: ... was added to the purified base after concentrating and incubated for 10 min on ice before addition of 300 μM dybenzocyclooctane (DBCO)-Cy3 (Click Chemistry Tools) or LD655 (Lumidyne Technologies) ...
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bioRxiv - Bioengineering 2019Quote: ... 2,5-dioxopyrrolidin-1-yl 1-azido-3,6,9,12-tetraoxapentadecan-15-oate (azido-PEG4-NHS Ester) was purchased from Click Chemistry Tools (Scottsdale, AZ). Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA) ...
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bioRxiv - Biophysics 2023Quote: ... Azide-PEG4-NHS ester (Cat# AZ103-100) and Sulfo-DBCO NHS ester (Cat# A124-10) were purchased from Click Chemistry Tools (Scottsdale, AZ). Alexa 488 Mouse Anti-ZAP70 (pY319)/Syk (Y352 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μM Cy3 azide (Click Chemistry Tools AZ119-1), and 100 mM ascorbic acid freshly prepared and diluted in Gomori buffer (pH 7.4) ...