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Citations for Click Chemistry Tools :
1 - 50 of 60 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (200 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (11 μL, 110 μmol, Click Chemistry Tools) followed by DIEA (38 μL ...
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bioRxiv - Bioengineering 2021Quote: ... Cy5.5 maleimide (purchased from Click Chemistry Tools, for Cys-Cy5.5 conjugate 9), Cy5.5-NHS ester (purchased from Click Chemistry Tools ...
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bioRxiv - Molecular Biology 2019Quote: Biotin was attached to metabolically labeled RNAs in a 10–20 μl reaction with 4 mM biotin disulfide azide (Click Chemistry Tools), 5 mM CuSO4 (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... 1.6 mM BTTAA ligand (2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)−1 H-1,2,3-triazol-1-yl)acetic acid) (Click Chemistry Tools Scottsdale, Az), and 5 mM sodium ascorbate ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3’-amino modified oligos (Supplementary Table S5) were activated by 1 mM NHS-methyltetrazine (Click Chemistry Tools) in 50% DMSO for 60 min at 21 °C ...
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bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (100 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (3.7 μL, 37.2 μmol, Click Chemistry Tools) followed by DIEA (13 μL ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in 50 μl of CuAAC reaction mixture (128 μM TBTA, 1 mM CuSO4, 1.2 mM freshly prepared sodium ascorbate, and 20 μM AFDye 488 Azide Plus fluorophore [Click Chemistry Tools] in PBS + 0.1% Triton X-100 + 0.01% BSA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 50 μl of CuAAC reaction mixture (128 μM TBTA, 1 mM CuSO4, 1.2 mM freshly prepared sodium ascorbate, and 20 μM AFDye 488 Azide Plus fluorophore [Click Chemistry Tools] in PBS + 0.1% Triton X-100 + 0.01% BSA ...
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bioRxiv - Bioengineering 2021Quote: ... 2-(4-((bis((1-(tert-butyl)-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)acetic acid (BTTAA, Click Chemistry Tools > 95%), Click-IT™ Fucose Alkyne (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µM O-propargyl-puromycin (OPP, Click Chemistry Tools) was added to the media ...
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bioRxiv - Bioengineering 2020Quote: ... The reduced C-terminal cysteine (1 eq.) was reacted with sulfo DBCO-maleimide crosslinker (4 eq.) (Click Chemistry Tools, AZ, USA) in PBS (pH 6.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were then washed with ice-cold D-PBS 4 times and incubated in D-PBS supplemented with 1 μM Cy5.5-azide (Click Chemistry Tools), 1 mM TCEP (adjusted to pH 7.0) ...
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bioRxiv - Bioengineering 2021Quote: ... 5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA; Click Chemistry Tools), 2 mM copper sulfate ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µM 5-Ethynyl-2′-deoxyuridine (EdU; Click Chemistry Tools 1149) was added to the cell culture media for 30 minutes to label NSCs in S-phase ...
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bioRxiv - Neuroscience 2020Quote: ... 3) 8 ul of 5mM Biotin-Alkyne (C21H35N3O6S, Click Chemistry Tools), and 4 ...
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bioRxiv - Developmental Biology 2019Quote: ... 4 mM disulfide biotin azide (Click Chemistry Tools, Scottsdale, AZ) was added to a 10 μL reaction containing RNA ...
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bioRxiv - Biochemistry 2023Quote: ... Tris(3-hydroxypropyltriazolyl-methyl)amine (THPTA) was purchased from Click Chemistry Tools. 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) ...
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bioRxiv - Bioengineering 2022Quote: ... poly(ethylene glycol) methyl ether DBCO (20 kDa, A120-100, Click Chemistry Tools), poly(ethylene glycol ...
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bioRxiv - Bioengineering 2022Quote: ... and 5-days post-surgery along with 5-Ethynyl-2′-deoxyuridine (EdU, Click Chemistry Tools, 3 mg/kg) every day (n = 4-5/group) ...
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bioRxiv - Bioengineering 2021Quote: ... while 30 μL of 200 μM cDNAs were incubated with 1.2 μL of 100 mM UV-cleavable DBCO-NHS ester (20 equivalents to cDNAs; Click Chemistry Tools) in 30% DMF for 4 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... Antibodies were then co-incubated with a 20-fold molar excess of NHS ester: PC-biotin-NHS ester (Click Chemistry Tools), Biotin-PEG2-NHS ester (BroadPharma) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 44 µL of a 1:1 mixture of BTTAA (Click Chemistry Tools, 77.4 mg mL-1 PBS) and copper sulfate pentahydrate (7.4 mg mL-1 water) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: PTH peptides with C terminal Cys residues purified by HPLC were incubated with 3 molar equivalents of DBCO-maleimide (Click Chemistry Tools #A108-100) and the DBCO-peptide products purified by HPLC ...
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bioRxiv - Microbiology 2023Quote: ... and then resuspended to OD600 of 0.6 in 1 mL PBS + 1 μM AZDye 488 DBCO (Click chemistry tools, cat# 1278-1). Cells were covered with aluminum foil and rocked for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... RPAAZP (∼ 4 µM in 5 mL of storage buffer) was mixed with 1.5-fold molar excess DBCO-MB543 (Click Chemistry Tools Inc., AZ) or DBCO-Cy3 ...
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bioRxiv - Bioengineering 2023Quote: 7-(Diethylamino)-4- (hydroxymethyl)coumarin (DEAC-OH) was previously synthesized in-house (Method S3) and conjugated to 2-azidoacetic acid (Click Chemistry tools, 1081), 3-azidopropionic acid (Synthonix ...
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bioRxiv - Biochemistry 2023Quote: ... 330 μL TCEP (14.0 mg/mL in H2O) and 160 μL of either heavy or light isoDTB tags (4 mg in DMSO, Click Chemistry Tools, 1565). The samples were then treated with 120 μL of the heavy (DMSO treated ...
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bioRxiv - Biochemistry 2024Quote: ... 165 µL TCEP (14.0 mg/mL in H2O) and 160 µL of either heavy or light isoDTB tags (4 mg in DMSO, Click Chemistry Tools, 1565). The samples were then treated with 120 µL of the heavy (DMSO treated ...
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bioRxiv - Cell Biology 2024Quote: ... CalFluor 647 Azide(Click chemistry tools, 1272-1); Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric ...
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bioRxiv - Bioengineering 2019Quote: ... 2,5-dioxopyrrolidin-1-yl 1-azido-3,6,9,12-tetraoxapentadecan-15-oate (azido-PEG4-NHS Ester) was purchased from Click Chemistry Tools (Scottsdale, AZ). Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μM Cy3 azide (Click Chemistry Tools AZ119-1), and 100 mM ascorbic acid freshly prepared and diluted in Gomori buffer (pH 7.4) ...
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bioRxiv - Developmental Biology 2022Quote: ... The click reaction was performed for 1 hour in click staining solution (100 mM Tris pH 8.5, 1 mM CuSO4, 2 mM AZDye-488 Azide (Click Chemistry Tools, 1275-1), and 100 mM ascorbic acid) ...
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bioRxiv - Cancer Biology 2023Quote: ... Monoclonal antibodies at 1 mg mL-1 in 1X PBS were incubated with DBCO-PEG5-NHS Ester linker (A102P, Click Chemistry Tools, Scottsdale, AZ, USA) at a 4:1 molar ratio of crosslinker to antibody for at least 2 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and 24 μM carboxyrhodamine 110 Alkyne (Click Chemistry Tools, TA106-1). Cells were washed 3x in PBS before acquisition via FACS or ImageStream.
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bioRxiv - Neuroscience 2020Quote: ... with 25 μM Alkyne Alexa fluor 405 (Click Chemistry tools # 1309-1), CuSO4 and additive overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... and 100× molar equivalents of DBCO-AF647 (Click Chemistry Tools, 1302-1) in DMSO were added and the reaction was mixed at 500 rpm in the dark for 2 hours rt ...
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bioRxiv - Bioengineering 2020Quote: ... incubated for 40 minutes in AFDye 488 DBCO (Click Chemistry Tools, 1278-1; 30 μM) at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... MCF10A cells were treated with 1 mM 5-ethynyl uridine (5-EU; Click Chemistry Tools 1261) for 1 h to label nascent RNA as in (Bryant et al ...
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bioRxiv - Bioengineering 2023Quote: ... they were reacted with 10:1 Methyltetrazine-sulfo-N-Hydroxysuccinimide ester (Met-NHS) (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Biophysics 2024Quote: ... IEDDA reaction was performed by applying 1-2 μM tetrazine dye (Click Chemistry Tools, Scottsdale, AZ) to the culture medium and incubating it with the cells at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... εmax = 250,000 M-1cm-1 for Cy5 as provided in the product specifications by Click Chemistry Tools. MWFis1ΔN = 13,584 daltons and MWDrp1 = 81,877 daltons.
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bioRxiv - Cell Biology 2022Quote: ... In every replicate 1 – 2 mg BTD-labeled lysate was clicked to Az800 (AzDye 800 – Click Chemistry tools) as described above ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were pulse-labeled for 60 min by supplementing 1 mM L-AHA (Click Chemistry Tools, 1066-100) or L-methionine (Sigma ...
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bioRxiv - Bioengineering 2019Quote: ... The suspension was centrifuged and resuspended in 1% Tween 80 containing PBS with methyltetrazine-PEG4-Maleimide (Click Chemistry Tools) of 200 μM final concentration ...
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bioRxiv - Bioengineering 2023Quote: ... and 2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl) acetic acid (BTTAA) (catalog no. 1236) were purchased from Click Chemistry Tools. Iodoacetamide and 4,5-methylenedioxy-1,2-phenylenediamine dihydrochloride (DMB ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 µl of methanol-preserved cells (∼30’000 cells in total) were mixed with 1 mM NHS-TCO (Click Chemistry Tools) and incubated in the dark at 21 °C for 5 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... two HBSS washes were performed and hydrogels were then incubated in AFDye 488 DBCO (Click Chemistry Tools, 1278-1; 30 μM) for 40 minutes at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μL of 1 mg/mL antibodies were reacted with 1 μL of 10 mM UV-cleavable azido-NHS ester (30 equivalents to antibodies; Click Chemistry Tools) for 2 h ...