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Citations for Click Chemistry Tools :
101 - 119 of 119 citations for 5 NITRO BENZO B THIOPHENE 2 CARBOXYLIC ACID METHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and the click reaction was initiation by addition of 2.75µl of click chemistry master mix (0.5µl of 5mM azido-rhodamine or tetramethylrhodamine-5-carbonyl azide [Click Chemistry Tools, Scottsdale ...
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bioRxiv - Cell Biology 2024Quote: ... Click-labeling of nascent proteins was performed with 8.3 µM dibenzocyclooctyne (DBCO)−488 (Click Chemistry Tools Catalog #1278-5) in PBS for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... One mg of cell lysate was resuspended in 445µl 1x SDS buffer with 1x EDTA-free protease inhibitors and incubated for 1.5 hours with 55µl of click reaction master-mix consisting of 10µl of 5mM diazo biotin azide (Click Chemistry Tools), 10µl of 50mM TCEP ...
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bioRxiv - Cell Biology 2022Quote: ... In every replicate 1 – 2 mg BTD-labeled lysate was clicked to Az800 (AzDye 800 – Click Chemistry tools) as described above ...
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bioRxiv - Neuroscience 2020Quote: ... Atxn2Q100/+ and HttQ111/+ mice in the 5.5- and 12-month treatment cohorts were IP injected with 180 mpk 5-ethynyl uridine (Click Chemistry Tools) 5 hours prior to sacrifice via lethal IP injection of at least 250 mpk sodium pentobarbital containing euthanasia solution ...
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bioRxiv - Biochemistry 2022Quote: ... RPA-DBD-A4AZP or RPA-DBD-D4AZP (∼4 mM in 5 ml storage buffer) was mixed with 1.5-fold molar excess DBCO-Cy5 or DBCO-Cy3 (Click Chemistry Tools Inc.) and incubated for 2 hours at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... with an azide or alkyne Alexa Fluor 488/594 (AF 594 Alkyne, 1297-1 or AF 488 Picolyl Azide 1276-5, Click Chemistry Tools, USA) for 1 hour in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... RPAAZP (∼ 4 µM in 5 mL of storage buffer) was mixed with 1.5-fold molar excess DBCO-MB543 (Click Chemistry Tools Inc., AZ) or DBCO-Cy3 ...
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bioRxiv - Genetics 2024Quote: ... permeabilized with 0.5% TritonX-100 for 15 minutes at room temperature and subjected to click labeling reaction using AFDye 488 Azide (Click Chemistry Tools, #1314). The AFDye 488-labeled cells were analyzed by flow cytometry using the FITC channel.
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bioRxiv - Plant Biology 2023Quote: ... 44.5 μL of labelled proteins were incubated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Plant Biology 2024Quote: ... 44.5 μl of labelled proteins were treated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Genomics 2022Quote: 100 µl of 100 µM oligo barcode (IDT) in PBS and 10 µl of 10 mM azide-NHS (Click Chemistry Tools cat# 1251-5) in DMSO were mixed at room temperature for two hours ...
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bioRxiv - Molecular Biology 2021Quote: ... Azide-modified antibody was first purified from the sortase reaction mixture using an Amicon 100kDa MWCO centrifugal filter and then reacted with a 5-fold excess of DBCO-AlexaFluor647 (Click Chemistry Tools, Scottsdale, AZ). Fluorescently labeled antibody was purified using centrifugal filtration ...
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bioRxiv - Immunology 2020Quote: ... A 500 μL labeling reaction containing 0.5 mg of ovalbumin in 1X BBS and 50 μL of 2 mM mTz-PEG4-NHS in DMSO (0.2 mM final; Click Chemistry Tools, 1069), pH 8.5 was rotated at 4 °C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... The click reaction was performed for 1 hour in click staining solution (100 mM Tris pH 8.5, 1 mM CuSO4, 2 mM AZDye-488 Azide (Click Chemistry Tools, 1275-1), and 100 mM ascorbic acid) ...
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bioRxiv - Physiology 2023Quote: ... strain-promoted inverse electron-demand Diels-Alder cycloaddition (SPIEDAC) was performed by adding 1-2 μM tetrazine dye (Click Chemistry Tools, Scottsdale, AZ) to the culture medium and incubating it with the cells at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: The Click reaction was then performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy5 picolyl azide (Click Chemistry Tools 1171-1)) for 1 hour at 37°C in the dark ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates containing 50 μg of protein were then incubated for 2 h with shaking at 37°C with 100 μM TAMRA azide (Click Chemistry Tools Scottsdale, Az), 800 μM copper(II ...
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bioRxiv - Molecular Biology 2022Quote: ... in situ click reactions were performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy3 picolyl azide (Click Chemistry Tools 1178-1)) for 1 hour at 37°C in the dark ...