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Citations for Click Chemistry Tools :
51 - 73 of 73 citations for 3 Methoxy 2 2 4 4 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... both transfected and untransfected control cells were treated with 50 µM O-Propargyl Puromycin (OPP, Click Chemistry Tools) for 10 min at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: Scaffolds from each experimental group were cultured in media with 50 µM of Ac4GalNAz (Click Chemistry Tools, Scottsdale, AZ) for 3 days (days 4-7 of culture) ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3’-amino modified oligos (Supplementary Table S5) were activated by 1 mM NHS-methyltetrazine (Click Chemistry Tools) in 50% DMSO for 60 min at 21 °C ...
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Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical NanoporesbioRxiv - Biophysics 2023Quote: ... Sortase labeling was performed following published protocols at 50-fold excess of the dye-triglycine conjugate (Click Chemistry Tools, USA) for 1 h at room temperature in Tris buffer(50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... via copper(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition (CuAAC) “click” reaction as follows: 100 mM CuSO4 and 50 mM BTTAA (#1236, Click Chemistry Tools) were premixed at 1:5 ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was terminated by quenching with a 10 mM Tris-HCl [pH 8.0] buffer supplemented with 50 µM methyltetrazine-DBCO (Click Chemistry Tools). The methanol-preserved cells conjugated with ClickTags were rehydrated following Supplementary Protocol 3.
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bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (100 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (3.7 μL, 37.2 μmol, Click Chemistry Tools) followed by DIEA (13 μL ...
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bioRxiv - Microbiology 2021Quote: ... 15 mL portions of each sample were resuspended in 15 mL of incubation media amended with 1 mM homopropargylglycine (HPG; Click Chemistry Tools, USA) (HPG-positive microcosm) ...
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bioRxiv - Cell Biology 2024Quote: ... aggregates were embedded as described above and cultured in CK+DCI media with 50 µM azide-modified galactosamine (Click Chemistry Tools Catalog #2281). Click-labeling of nascent proteins was performed with 8.3 µM dibenzocyclooctyne (DBCO)−488 (Click Chemistry Tools Catalog #1278-5 ...
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bioRxiv - Bioengineering 2024Quote: ... whereas PEG-BCN was preincubated for 15 minutes with 50 µM of AlexaFluor 568 azide (1:200 dye:PEG) (Click Chemistry Tools; Scottsdale, AZ). DN hydrogels were then formed with these fluorescently tagged components ...
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bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (200 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (11 μL, 110 μmol, Click Chemistry Tools) followed by DIEA (38 μL ...
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bioRxiv - Immunology 2024Quote: ... SAv-drug conjugation reactions were next incubated for 1 hour with 100 μL azide agarose slurry with gentle mixing (50 μL packed resin; Click Chemistry Tools/Vector Laboratories), after which the resin was removed using Spin-X columns (Corning) ...
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bioRxiv - Biophysics 2023Quote: ... 50ul of 1mg/ml of BSA-Azide (Click chemistry tools, 1535) was incubated with 15ul of 100uM (5’ ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: PTH peptides with C terminal Cys residues purified by HPLC were incubated with 3 molar equivalents of DBCO-maleimide (Click Chemistry Tools #A108-100) and the DBCO-peptide products purified by HPLC ...
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bioRxiv - Cell Biology 2020Quote: ... and 100 μg/mL cycloheximide and incubated with 100 μM L-azidohomoalanine (AHA; Click Chemistry Tools) and 10 μM methionine-free amino acid mixture (Promega) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 44 µL of a 1:1 mixture of BTTAA (Click Chemistry Tools, 77.4 mg mL-1 PBS) and copper sulfate pentahydrate (7.4 mg mL-1 water) ...
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bioRxiv - Developmental Biology 2020Quote: ... ESC were treated with DOX (different concentrations in the range from 150–600 ng/ml) or 500 µM auxin as indicated or incubate with 10µM EdU (Click Chemistry Tools) for 30 minutes before fixation with 4% PFA in PBS for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μL of 1 mg/mL antibodies were reacted with 1 μL of 10 mM UV-cleavable azido-NHS ester (30 equivalents to antibodies; Click Chemistry Tools) for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... RPA-DBD-A4AZP or RPA-DBD-D4AZP (∼4 mM in 5 ml storage buffer) was mixed with 1.5-fold molar excess DBCO-Cy5 or DBCO-Cy3 (Click Chemistry Tools Inc.) and incubated for 2 hours at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... and then resuspended to OD600 of 0.6 in 1 mL PBS + 1 μM AZDye 488 DBCO (Click chemistry tools, cat# 1278-1). Cells were covered with aluminum foil and rocked for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: 10 μM of RPA4AZP (0.5 mL) was mixed with 2X molar excess of the dibenzocyclooctyne-MB543 (DBCO-MB543; Click Chemistry Tools Inc.) dye and incubated at 4 °C for 1.5 h with gentle rocking in dark ...
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bioRxiv - Cancer Biology 2024Quote: ... protein lysates from OSU-CLL cells (1e6 cells/mL) were clicked to TAMRA biotin-azide and incubated with streptavidin agarose resin (Click Chemistry Tools; Scottsdale, AZ) to isolate biotin-alkyne-tagged proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... Monoclonal antibodies at 1 mg mL-1 in 1X PBS were incubated with DBCO-PEG5-NHS Ester linker (A102P, Click Chemistry Tools, Scottsdale, AZ, USA) at a 4:1 molar ratio of crosslinker to antibody for at least 2 h at room temperature ...