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Citations for Seikagaku :
1 - 50 of 54 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or Chondroitin Sulfate standard (Seikagaku Corp.), respectively.
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bioRxiv - Bioengineering 2024Quote: ... Chondroitin Sulfate D (400676, CS-D, shark cartilage, average molecular mass of 38 kDa27) was acquired from Seikagaku. Chondroitin Sulfate E (CS-E ...
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bioRxiv - Cell Biology 2024Quote: ... 1.2 M sorbitol) containing 200 µg/ml zymolyase 100T (120493; Seikagaku), and incubated for 60 min at 30 °C ...
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bioRxiv - Biochemistry 2024Quote: ... cells were resuspended in 6.7 ml per g wet weight MP2 buffer (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase 20T from Seikagaku Biobusiness) and incubated for 1 h at 30°C ...
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bioRxiv - Genomics 2024Quote: ... and permeabilized with 1 mg/mL Zymolyase 100 T (Seikagaku) in PEMS buffer at 37°C for 30 minutes ...
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bioRxiv - Biophysics 2024Quote: ... N° 10837059001) or from Seikagaku Corp ...
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bioRxiv - Biophysics 2024Quote: HEWL powders from Seikagaku Corp ...
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bioRxiv - Biochemistry 2023Quote: ... yeast cells were resuspended in 6.7 ml per g wet weight MP2 buffer (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase 20T from Seikagaku Biobusiness) and incubated for 1 h at 30°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the resuspended cells were mixed with 15 mg of Zymolyase 100T (Seikagaku Corp.) that was dissolved in 30 ml 1 M sorbitol ...
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bioRxiv - Molecular Biology 2023Quote: ... VCAN (1:200, Mouse Anti-Versican Antibody 2B1, Seikagaku), and ELN (1:400 ...
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bioRxiv - Molecular Biology 2023Quote: ... DCN (1:1500, Mouse Anti-Dermatan Sulfate Proteoglycan Antibody 6B6, Seikagaku), VCAN (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... H-89 was purchased from Seikagaku Corp ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mU heparinase III (Seikagaku Corp., Tokyo, Japan), 31.3 mM sodium acetate ...
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bioRxiv - Cell Biology 2022Quote: ... containing 1 mU heparinase I (Seikagaku Corp., Tokyo, Japan), 1 mU heparinase II (Iduron ...
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bioRxiv - Biochemistry 2022Quote: ... 20 µL of β-mercaptoethanol and 20 mg of Zymolyase 100T (Seikagaku Kogyo Co., Tokyo, Japan) were added and incubated at room temperature for 15 min to degrade the cell wall ...
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bioRxiv - Genetics 2022Quote: ... Zymolyase (100T, Seikagaku) was added at a final concentration of 3.3 μg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated in 98 μl Sorbitol buffer + 2 μl of 2.5 mg/ml Zymolyase 100T (SEIKAGAKU CORPORATION) for 90 min (wild type ...
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bioRxiv - Biochemistry 2022Quote: ... yeast cells were resuspended in 6.7 ml per g wet weight MP2 buffer (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase from Seikagaku Biobusiness) and incubated for 1 h at 30°C ...
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bioRxiv - Cell Biology 2022Quote: ... pH 5.8 the cells were resuspended in the same buffer containing 0.25 mg/ml Zymolyase 20T (Seikagaku Corp., Tokyo) and incubated for 2 h at 30 °C ...
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bioRxiv - Cell Biology 2022Quote: ... were suspended in 20 mL ice-cold 50 mM Tris–HCl (pH 7.4) buffer containing 1 mg/mL lysozyme (Seikagaku Corporation, 100940) and 1× protease inhibitor cocktail (Nacalai Tesque ...
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bioRxiv - Neuroscience 2022Quote: ... ChondroitinaseABC (10 U/ml) (Seikagaku Corporation), was first diluted 10-fold and then 1/2000 of it was added to the medium to a final concentration of 0.5 mU/ml in the culture medium (Neurobasal supplemented with B-27).
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bioRxiv - Microbiology 2022Quote: ... cells were treated with 50 mg/mL zymolyase 20T (Seikagaku Corporation, Japan) in SB buffer for 30 min at 30 °C ...
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bioRxiv - Microbiology 2022Quote: ... cells were collected by centrifugation at 5,000 x g for 5 min and treated with two units of Zymolyase (Seikagaku Corporation, Japan) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and Chondroitin Sulfate standard (Seikagaku Corp.), respectively.
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bioRxiv - Cell Biology 2022Quote: ... 20T Zymolyase (Seikagaku Biobusiness, product code 120491) at 3 mg/g cell pellet] using 6.7 ml per gram of cell pellet ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were treated for 30min with 0.5 mg ml-1 Zymolyase 100T (Seikagaku Co., Tokyo) in ZK buffer at room temperature ...
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bioRxiv - Genomics 2021Quote: ... After adding 5 to 10 µl Zymolyase 20T (Seikagaku, Tokyo, Japan, 25 mg/ml) and 5 to 10 µl lyzing enzyme (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were resuspended in 500 μl SP buffer with 0.1% β-mercaptoethanol and 10 μl of 20 mg/ml Zymoylase-20T (Seikagaku). The cells were incubated with soft rotation for 20 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were treated with 50 µg/mL of Zymolyase 20T (Seikagaku Inc., Tokyo Japan) for 30 min to remove the yeast cell wall ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 200 μl of ice-cold washing buffer and subsequently cell wall was digested by incubating 100 μl of cells with 0.4 mg/ml of Zymolyase 100T (SEIKAGAKU CORPORATION) at 30 °C for 5-15 min ...
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bioRxiv - Biochemistry 2021Quote: ... The volume of concentrated samples was adjusted to 70 ul and 3 ul of Chondroitinase ABC (1.4 U/ml Stock solution, containing BSA, Seikagaku) was added to each sample ...
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bioRxiv - Microbiology 2021Quote: ... washed with sorbitol buffer (1M sorbitol, 0.1M NaH2PO4, pH 8.0) and the cell wall was digested with Zymolyase 20T (Seikagaku Biobusiness Corporation) for about 35 min at 37° C ...
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bioRxiv - Biochemistry 2021Quote: ... residual proteoglycans and procollagens in the calf AF residue were extracted further by treating first with 0.125 unit/ml chondroitinase ABC (EC4.2.2.4) (Seikagaku Kogyo Co., Tokyo, Japan) in 4 ml of 0.05 M-Tris/ HC1/0.06M-sodium acetate buffer ...
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bioRxiv - Genomics 2021Quote: ... 0.2 mg/ml zymolyase 20T (Seikagaku) and incubated for 90 min at 37 °C ...
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bioRxiv - Genomics 2021Quote: ... 10 mM EDTA (pH 8.0) containing 0.125 mg of Zymolyase 20T (Seikagaku) at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... before resuspending in 6.7 ml per g wet weight in MP2 (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase from Seikagaku Biobusiness) and incubated at 30°C for 60 min ...
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bioRxiv - Immunology 2021Quote: ... The endotoxin level of HMGN1 (< 1 endotoxin unit per mg) was assessed by Endospecy ES-50M Kit (Seikagaku Corporation, Japan). The protein sequence of HMGN1 was identified by Applied Biosystems Procise 492 HT (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... chondroitin sulfate D (CS-D, Mw = 20 kDa) from whale cartilage (Seikagaku, Tokyo, Japan) and chondroitin sulfate E (CS-E ...
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bioRxiv - Microbiology 2020Quote: ... and chondroitin sulfate E (CS-E, Mw = 20 kDa) from squid cartilage (Seikagaku). Keratan sulfate (KS ...
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bioRxiv - Cell Biology 2020Quote: ... heparitinase I at 10 mU/mL (Seikagaku, 100704), chondroitinase ABC at 100 mU/mL (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... GAG concentrations were calculated from a standard curve produced with chondroitin sulphate (Seikagaku Chemicals). DNA was assessed from neutralized samples using buffered Hoechst solution (0.667 μg/ml in 0.2 M pH 8.0 phosphate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... ChABC (50 U/ml in PBS, Seikagaku), or AAV vectors (AAV1-PGK-Chst3 or AAV1-PGK-GFPor AAV1-SYN-GFP ...
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bioRxiv - Neuroscience 2020Quote: ... 0.5 μg of AMAC-conjugated disaccharide samples and standard disaccharides (hyaluronic acid – HA, non-sulfated chondroitin - C0S, C4S, C6S; Seikagaku) were separated on a 30% polyacrylamide gel in Tris Glycine buffer for 30-40 min.
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bioRxiv - Neuroscience 2020Quote: ... the samples were further treated further with 50 mU of chondro-6-sulfatase (Seikagaku). Samples were centrifuged ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... anti-Heparan Sulfate-ProteoGlycans (10E4, 5µg/mL, Seikagaku corporation) and anti-p24 gag RD1 (KC57 ...
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bioRxiv - Cell Biology 2020Quote: ... then yeast cell walls were digested by adding 100 µL Zymolyase (10 mg/mL, Seikagaku) for 1 hour at RT ...
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bioRxiv - Biochemistry 2020Quote: ... Spheroplasts were prepared as described (Daum et al., 1982) using Zymolyase 100T (Seikagaku; 0.5 mg/g cells wet weight). The spheroplasts were resuspended in ice-cold D-buffer (0.6 M sorbitol ...
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bioRxiv - Biochemistry 2020Quote: ... chondroitin sulfate D (CSD, Mw = 20 kDa) from whale cartilage (Seikagaku, Tokyo, Japan) and chondroitin sulfate E (CSE ...
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bioRxiv - Biochemistry 2020Quote: ... and chondroitin sulfate E (CSE, Mw = 20 kDa) from squid cartilage (Seikagaku). N-desulfated HP (N-DeS HP ...
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bioRxiv - Molecular Biology 2020Quote: ... Genomic DNA were prepared in the agarose plug by 0.4 mg/ml Zymolyase (20T, Seikagaku) treatment in Zymolyase buffer at 37°C for 1h ...