Labshake search
Citations for Bioo Scientific Corporation :
51 - 100 of 127 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2021Quote: ... Each RNA sequencing library was prepared using the NEXTflex Rapid RNAseq kit (Bioo Scientific). Libraries were quantified with quantitative PCR (NEBnext Library quantification kit ...
-
bioRxiv - Cancer Biology 2022Quote: ChIP-seq libraries were prepared using NEXTflex ChIP-Seq Kit (#5143-02, Bioo Scientific) following the manufacturer’s protocol (V12.10 ...
-
bioRxiv - Physiology 2022Quote: ... Libraries were generated with a NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) paired with the NEBNext Methylated Adaptor (New England Biolabs) ...
-
bioRxiv - Biophysics 2022Quote: ... Each RNA sequencing library was prepared using the NEXTflex Rapid RNAseq kit (Bioo Scientific). Libraries were quantified with quantitative PCR (NEBnext Library quantification kit ...
-
bioRxiv - Microbiology 2022Quote: ... Genomic DNA library was generated using NEXTflex Rapid DNA Seq kit (Bioo Scientific, USA) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... and libraries were prepared using NEXTflex™ Dual-Index adapters (Bioo Scientific, Austin, Texas) in an automated fashion (SPRIworks Fragment Library System II ...
-
bioRxiv - Plant Biology 2021Quote: ... and libraries were prepared with the NEXTflex Small RNA Sequencing Kit v2 (Bioo Scientific) in combination with rRNA depletion with customized biotinylated antisense oligonucleotides (Chotewutmontri et al. ...
-
bioRxiv - Plant Biology 2020Quote: ... Barcoded sequencing libraries were prepared using NextFlex Rapid DNA-Seq Kits (Bioo Scientific, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2022Quote: ... mRNA libraries were prepared using the NEXTflex Rapid Directional RNAseq Kit (BioO Scientific Corp.). Fragmentation of the mRNA was achieved by incubating 95°C for 13 minutes to produce ∼300 bp fragments ...
-
bioRxiv - Microbiology 2023Quote: ... The NEXTFLEX Rapid DNA-Seq Library Prep Kit 2.0 (Bioo Scientific, Austin, TX, USA) was used for DNA library preparation with an insert size of ∼300 bp according to the manufacturer’s recommendations ...
-
bioRxiv - Developmental Biology 2023Quote: ... cDNA fragments were ligated to Bioo Scientific NEXTflex dual adapters (Bioo Scientific, Austin, TX, USA). Quality and quantity of the finished libraries were assessed using a combination of Agilent DNA High Sensitivity chip (Agilent Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... The NEXTflex library preparation was run PCR-free according to the manufacturer’s recommendations (Bioo Scientific NEXTflex PCR Free DNA Sequencing Kit – 5142-01) ...
-
bioRxiv - Molecular Biology 2021Quote: ... ChIP-seq libraries were prepared using a NEXTflex ChIP-seq kit (5143-02, Bioo Scientific) according to the manufacturer’s guidelines ...
-
bioRxiv - Microbiology 2020Quote: ... The library was constructed using the NEXTflex Rapid DNA-Seq kit (Bioo Scientific, Austin, TX), the quality of sequencing reads was analyzed using FastQC (Babraham Bioinformatics) ...
-
bioRxiv - Genomics 2019Quote: ... The sequencing libraries were prepared by the NEXTflex Rapid DNA-seq kit (Bioo Scientific Corporation). All sequencing was conducted at the NIEHS Epigenomics Core Facility (NextSeq 500 or NovaSeq 6000 NGS system ...
-
bioRxiv - Genetics 2019Quote: Illumina-compatible libraries were prepared as previously described26 using NEXTflex-96 barcode adapters (Bioo Scientific) and Ampure XP beads (Beckman Coulter) ...
-
bioRxiv - Genomics 2022Quote: ... RNA-seq libraries were prepared using the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific) following the manufacturer’s instructions and sequenced using 75 bp single-end sequencing on the Illumina NextSeq 500 platform by the BioFrontiers Institute Next-Gen Sequencing Core Facility.
-
bioRxiv - Plant Biology 2022Quote: ... barcoded adapter ligation and library amplification according to NEXTFLEX ChIP-Seq Kit guidelines (BIOO Scientific). Finally ...
-
bioRxiv - Microbiology 2022Quote: ... Paired-end library was constructed using NEXTFLEX Rapid DNA-Seq (Bioo Scientific, Austin, TX, USA). Adapters containing the full complement of sequencing primer hybridization sites were ligated to the blunt-end of fragments ...
-
bioRxiv - Microbiology 2024Quote: ... Paired-end library was constructed using NEXTflexTM Rapid DNA-Seq (Bioo Scientific, Austin, TX, USA). Adapters containing the full complement of sequencing primer hybridization sites were ligated to the blunt-end of fragments ...
-
bioRxiv - Developmental Biology 2020Quote: ... and libraries were constructed using a NEXTflex™ ChIP-Seq Kit (Bioo Scientific, cat.: 5143-02). Fragments of 180–280 bp were size-selected using Agencourt AMPure XP beads (Beckman ...
-
bioRxiv - Molecular Biology 2021Quote: ... and libraries were prepared using a NEXTflex Rapid directional RNA-seq kit (5138-08, Bioo Scientific). The libraries were sequenced using an Illumina HiSeq 2500 platform ...
-
bioRxiv - Neuroscience 2020Quote: ... Strand specific libraries for each sample were prepared using the dUTP NEXTflex RNAseq kit (Bioo Scientific), which includes a magnetic bead-based size selection ...
-
bioRxiv - Cell Biology 2020Quote: ... NGS library preparation was performed with NEXTFLEX® Small RNA-Seq Kit v3 (Bioo Scientific®) following Step A to Step G of the manufacturer’s standard protocol (v16.06) ...
-
bioRxiv - Cancer Biology 2020Quote: ChIP-Seq libraries were prepared using the NEXTflex ChIP-Seq Kit (Bioo Scientific, ref. 5143-02) following the manufacturer’s protocol with some modifications ...
-
bioRxiv - Microbiology 2021Quote: ... and followed by amplification-free library preparation using NEXTflex PCR Free DNA prep kit (Bioo Scientific) according to standard manufacturer protocols ...
-
bioRxiv - Immunology 2020Quote: ... ChIP DNA libraries were ligated with the Bioo scientific barcoded adaptors (BIOO Scientific, Perkin Elmer, USA) with T4 DNA ligase according to KAPA LTP library preparation protocol and the ligated ChIP DNA libraries were purified with 1.8x vol ...
-
bioRxiv - Developmental Biology 2020Quote: ... mRNA enriched sequencing libraries were made with the NEXTflex Rapid Directional RNAseq Kit (BioO Scientific Corp.) and corresponding protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... and libraries were prepared using a NEXTflex Rapid directional RNA-seq kit (5138-08, Bioo Scientific). The libraries were sequenced using an Illumina HiSeq 2500 system ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Strand-specific transcriptomic libraries were built using NEXTflex Rapid Directional RNA-Seq Kit (Bioo Scientific, USA) based on the manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: ... A small RNA library was constructed using the NEXTFLEX Small RNASeq Kit (Bioo Scientific, TX, USA), targeting RNA molecules between 30 and 300 nt ...
-
Interspecific introgression and natural selection in the evolution of Japanese apricot (Prunus mume)bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were barcoded for each sample using single 8-bp NEXTflex adaptors (Bioo Scientific, Austin, TX, USA) and enriched by PCR using PrimeSTAR Max (Takara Bio ...
-
bioRxiv - Molecular Biology 2020Quote: 4tU-seq libraries were created using the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific #NOVA-5130) with a slightly modified protocol ...
-
bioRxiv - Immunology 2019Quote: ... Libraries were prepared using the Bioo Scientific NextFlex 16s V4 Amplicon-Seq kit (Bioo Scientific Corporation, TX). A first PCR amplification was performed using customized primers that target the V4 domain and a second PCR to integrate relevant flow cell binding domains and unique sample indices ...
-
bioRxiv - Genomics 2019Quote: ... A paired-end 170 bp insert library was constructed utilizing the NEXTflex PCR-Free approach (BIOO Scientific). The 2.5 kb library was constructed using the Mate Pair Library v2 Kit (Illumina) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Each sample library contained a unique barcode adapter (NEXTflex DNA Barcodes for Ion Torrent, BIOO Scientific, USA). We selected libraries within the size range of 450-500 bp using a 2% E-gel on an E-Gel Electrophoresis System (Invitrogen ...
-
bioRxiv - Developmental Biology 2020Quote: ... Libraries preparation for sequencing was performed by using NEXTflex ChIP-Seq Kit (Bioo Scientific, NOVA-5143-01) without size selection.
-
bioRxiv - Biochemistry 2019Quote: ... Sequencing libraries were prepared using magnetic beads similar to described previously using barcoded adapters (NextFlex, Bioo Scientific) (Garber et al. ...
-
bioRxiv - Microbiology 2022Quote: ... Each library was barcoded with unique dual index sequences (NEXTFLEX® Unique Dual Index Barcodes, BioO Scientific). Library size and amount were confirmed with a Bioanalyzer High Sensitivity DNA chip ...
-
bioRxiv - Neuroscience 2022Quote: ... miRNA libraries were generated with the NEXTFLEX Small RNA Sequencing Kit v3 (©Bioo Scientific Corp., 2019) from 3-20 ng of small RNA for each sample ...
-
bioRxiv - Microbiology 2023Quote: ... Paired-end sequencing libraries were prepared using NEXTFLEX™ Rapid DNA-Seq (Bioo Scientific, Austin, TX, USA). Paired-end sequencing was performed on an Illumina Novaseq 6000 system (Illumina Inc. ...
-
bioRxiv - Genetics 2024Quote: ... Fragmented DNA was used to produce barcoded DNA libraries using NEXTflex™ DNA Barcodes (Bioo Scientific, Inc.) with either the TruSeq® DNA Library Prep Kit (Illumina ...
-
Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: NGS libraries have been prepped from the ChIP DNA using the NEXTFlex ChIP-Seq kit from BioO Scientific and sequenced according to the Illumina TruSeq Rapid v2 protocol on the HiSeq2500 for a single read 50bp in length and a 8bp dual index read ...
-
bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries were constructed from mRNA using KAPA mRNA HyperPrep Kit (KK8580) and NEXFlex barcoded adaptors (Bioo Scientific). High-throughput sequencing was performed using a HiSeq 2500 instrument (Illumina ...
-
bioRxiv - Molecular Biology 2019Quote: The RNA-seq libraries were prepared using the NEXTflexTM Rapid Directional mRNA-Seq Kit (NOVA-5138, Bioo Scientific) according to manufacturer’s instructions ...
-
bioRxiv - Physiology 2019Quote: ... and strand-specific directional libraries were generated using the NEXTflex™ Rapid Directional RNA-Seq Kit (Bioo Scientific). For each genotype ...
-
bioRxiv - Cell Biology 2021Quote: ... Following Poly-A selection mRNA libraries were prepared according to manufacturer’s instructions (Bioo Scientific Corp., NOVA-5138-08), except that the RNA was incubated at 95°C for 13 min to generate optimal fragment sizes ...
-
bioRxiv - Developmental Biology 2021Quote: ... Following Poly-A selection mRNA libraries were generated according to manufactures instructions (Bioo Scientific Corp., NOVA-5138-08) except RNA was incubated for 13 min at 95°C to generate optimal fragment sizes ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA-Seq library preparation was carried out using NEXTflex Rapid Directional qRNA-Seq kit (Bioo Scientific, cat# 5130-05D). In brief ...
-
bioRxiv - Molecular Biology 2020Quote: ChIP-seq libraries were created using a combination of a NEXTflex Rapid DNA-Seq Kit (Bioo Scientific #NOVA-5144) and a NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific #NOVA-5130 ...