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Citations for Cell Signaling Technology :
251 - 300 of 762 citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the membrane was washed 3 times with TBS-T and incubated with horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology #7074) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed two times with PBS and incubated with 50 µL of primary antibody (α-LAMP1 rabbit, Cell Signaling, 1:100 in 1 % BSA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membrane was shaken three times for 5 min in TBST then incubated with 1:3,000 anti-rabbit HRP-linked antibody (Cell Signaling Technologies) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... per ChIP sample were washed three times with PBS + 0.01% Tween20 (collecting on a magnet at each step) and 10ul of anti-DNMT3A (Cell Signaling, #3598) added ...
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bioRxiv - Biochemistry 2019Quote: ... Membranes were then incubated with the appropriate secondary antibodies in TBS-T for 30 min at RT at 1:4000 dilution (Cell Signaling Technology, Anti-rabbit #7074, Anti-mouse #7076). Following washes with TBS-T ...
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bioRxiv - Molecular Biology 2020Quote: ... Blots were washed three times (total 30 min) and incubated with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technologies) for 1 h at room temperature with gentle rocking ...
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bioRxiv - Biophysics 2022Quote: ... Antigen recognition was performed by incubating primary antibody in a humidified chamber for different times (Phalloidin, Invitrogen, A12381; anti-Vinculin, Invitrogen, 42H89L44; anti-pFAK, Cell signaling, 3283S) and with anti-mouse/rabbit Alexa Fluor 488-594 (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were washed four times with TBST (5 minutes gentle rocking per wash) and incubated overnight with primary antibody Rabbit anti-Gli-1 (Cell signaling, #C68H3); 1:250 dilution in TBST-Milk at 4°C with gentle rocking ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was then washed an additional three times for five minutes each before incubating in a 1:2000 dilution of anti-mouse horseradish peroxidase conjugated antibody (Cell Signaling Technologies) in TBST ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were washed 3 times with PBS and incubated in secondary antibody (anti-mouse [Cell Signaling, #4408S]; anti-rabbit [Cell Signaling, #8889S]) for 1.5 hours ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were then washed three times in TBST and incubated with horseradish peroxidase-conjugated anti-Rabbit IgG secondary antibody (All antibodies from Cell Signaling Technology) at 1:2000 dilution in TBST containing 5% BSA ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed three times with PBS and incubated with a horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody (Cell Signaling #7074) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then rinsed three times with PBS before adding 100 ng/mL DAPI and fluorescent secondary antibody (Cell Signaling Technology 4413S) diluted 1:500 in PBS containing 1% BSA and 0.3% Triton-X for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were washed with tris-buffered saline with Tween 20 (TBST) three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S ...
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bioRxiv - Bioengineering 2022Quote: ... were washed with TBS buffer 3 times for 5 minutes and subjected to antigen retrieval using 1X SignalStain® Citrate Unmasking Solution (Cell signaling). The citrate buffer was bought to boiling temperature and added to the sections ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Cancer Biology 2020Quote: ... and the whole fibrin gel channel was collected and immersed for 10 minutes on ice in 25 µL of cell lysis buffer (fresh stock of 10 mL prepared each time from 1X buffer (9803S, Cell Signaling Technologies) with the addition of 1 µL of Benzonase Nuclease (E8263 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Immunology 2022Quote: ... Membranes were washed 3 times with TBST and then incubated with peroxidase-conjugated secondary antibody (Cell Signaling Technology, Cat.No. 7074S, 1:3000). The antigen-antibody reaction was visualized by enhanced chemiluminescence assay (ECL ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were spun down and washed three times with cold Flow Wash Buffer (FWB) (PBS, 2% FBS, 2mM EDTA) and incubated with primary antibody (EGFR-D1D4J, Cell Signaling Technology) diluted in FWB for 60 minutes on ice ...
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bioRxiv - Physiology 2022Quote: ... The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling, 4412S) at 1:1000 dilution at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue sections were then washed three times for 5 min with PB and mounted with a cover glass using ProLong (Cell Signaling Technology).
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bioRxiv - Genomics 2023Quote: ... The membrane was washed five times with TBST and then incubated for 1 h with secondary goat-anti-rabbit-HRP conjugate (1:4000, Cell Signaling Technology) in TBST with 5% non-fat powdered milk ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were harvested at indicated time points and lysed in 1× radioimmunoprecipitation assay (RIPA) buffer (Cell Signaling, Danvers, MA, USA; Cat# 501015489) containing 1× protease inhibitor cocktail (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed several times with TBST and then incubated with peroxidase-labeled goat anti-rabbit IgG (1:10,000; Cell Signaling-Beverly, Massachusetts). Bands were visualized with SuperSignal West Dura substrate (Pierce Biotechnology ...
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bioRxiv - Developmental Biology 2024Quote: ... They were washed three times with 1x TBST followed by incubation with the HRP-conjugated secondary antibody (1:2000) (Cell Signaling Technology) in 10 ml 1x TBST for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were then washed 3 times with PBST and were incubated with HRP-conjugated anti-rabbit IgG (1:500 dilution, catalog 7074S, Cell Signaling Technology) for 1 hr ...
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bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... Kit (Cell Signaling Technology, 3074), Ido1 (Santa Cruz Biotechnology ...
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bioRxiv - Genomics 2021Quote: ... Nuclear stain solution was prepared for 4 slides at a time: 20 µLSYTO13 (Nanostring) and 180 µL 1x TBS (Cell Signaling Technologies, 12498S) for a total volume of 200 µL/slide ...
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bioRxiv - Bioengineering 2021Quote: ... The plates were then washed 3 times with PBST before adding 100 μl of TMB substrate solution (Cell Signaling Technology, USA, Catalog: 7004S) to the plates after aspirating out the PBST and was incubated for 25 minutes at room temperature on a plate shaker ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times with PBS and incubated in the dark for 45 min with Alexafluor594 secondary antibody (1:100) (Cell Signaling, cat.# 8889S) in BSA/PBS 1 mg/ml and incubated in the dark for 45 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed three times for 5 min with PBS and incubated in anti-mouse IgG Alexa Fluor® 555 Conjugate (Cell signaling #4409) at a dilution of 1:500 for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S; Rat, Cell Signaling, cat #7077S ...
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bioRxiv - Cancer Biology 2019Quote: ... they were treated with 4 μM of H2CB for stated times then cells were fixed and stained for senescence-associated β-galactosidase (β-Gal) expression according to the manufacturer’s protocol (Cell Signaling Technologies).
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed three times with TBST and incubated in secondary antibodies accordingly: anti-mouse HRP-linked IgG (Cell Signaling Technology, 7076S) or anti-rabbit HRP-linked IgG (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The plates were washed three times with PBST and incubated with either 1 μg/ml of rabbit monoclonal anti-mouse TREM2 (Cell Signaling Technology, 76765) for full length Trem2 ELISA (ELISA 1) ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were washed three times for 5 min each with TBS-T then incubated in SignalFire ECL Reagent (Cell Signaling Technology, 6883) for 1 min and imaged using a BioRad ChemiDoc imager.
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested at indicated time points and lysed in 1x cell lysis buffer (Cell Signaling Technologies, Inc, Danvers, MA, USA; Cat#9803) or 1× radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Immunology 2023Quote: ... The membranes were then washed three times with TBST and incubated with HRP-linked anti-rabbit IgG antibody (Cell Signaling Technology, catalog # 7074) in TBST containing 5% BSA for 1 h at room temperature ...
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bioRxiv - Genetics 2024Quote: ... and then fixed at different time points (1 hour, 3 hours, 6 hours and 24 hours) before staining for DNA damage markers: ψH2AX (Cell Signaling Technology # 9718T), 53BP1 ((Novus Biologicals # NB100-904 ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were then washed three times in TBST and incubated with appropriate horseradish peroxidase–conjugated secondary antibodies at 1:5000 dilution in TBST (Cell Signaling Technology, US) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed by 1× TBST 3 times and incubated with a secondary anti-rabbit-HRP or anti-mouse antibody-HRP (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed three times with Tris-buffered saline containing 0.01% Tween 20 and incubated for one hour with secondary rabbit (Cell Signaling Technology, Inc, Danvers, Massachusetts) or goat anti-mouse HRP-conjugated antibody (Abcam Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... The amplification time was 100 minutes and the primary antibodies pair to detect the interaction was rabbit anti-Aurora-A (14475, Cell Signaling, 1:100 o.n.) and mouse anti-N-Myc (NCM II 100 ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were washed for 15 min in TBST four times then incubated for 2 hours at room temperature with an anti-rabbit IgG HRP-linked secondary antibody (Cell Signaling #7074; 1:20,000). Blots were imaged and quantified using ImageQuant (Amersham).
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bioRxiv - Physiology 2021Quote: ... the membranes were washed 3 times in TBS-T for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibodies (Cell Signaling Technology, London, UK) at dilution of 1:5000-1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, catalog # 7074) or goat anti-mouse IgG (1:3,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, Catalog # 7074) or goat anti-mouse IgG (1:3000 ...