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Citations for Cell Signaling Technology :
201 - 250 of 782 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... The vibraslices were then incubated for overnight at RT with rabbit anti-HA antibody (1:1000; Cell Signaling Technology Cat# 3724, RRID:AB_1549585), followed by 1 hr with the Cy5-conjugated goat anti-rabbit IgG ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% BSA in TBST buffer for 2 h at RT and incubated overnight at 4°C with primary antibodies against ERK1/2 (1:1000; 9102s, Cell Signaling Technology), pERK1/2 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and stained 1h/RT or overnight/4°C with following primary antibodies in 0,2% Triton-X100 in PBS: PAX6 (rabbit, CS60433, Cell Signaling, 1:300), ZO-1-1A12 (mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation for 1–3 hr at RT with 1:5000 secondary antibody (Cell Signaling Technology goat HRP-linked Anti rabbit IgG #7074), and 3 more 5 min washes with TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibody incubation was followed by incubation in HRP-conjugated secondary antibody for 1h RT at room temperature (goat anti mouse IgG and goat anti-rabbit IgG, Cell Signaling Technology). Bands were detected by chemiluminescence using ECL solution (WesternBright Sirius ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were blocked in 0.5% BR (Blocking Reagent (Akoya Biociences))/TNT (TN, 0.05% Tween) for 1h at RT and incubated with a primary antibody against pSMAD1,5,9 (Cell Signaling 13820S, rabbit, 1:300) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 m at RT and incubated in primary antibody rabbit anti–Calreticulin at 1:100 (Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... washed four times and then incubated with secondary antibody (Cell Signaling Technology, cat#7074 and cat#7076) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: Cells were washed three times and lysed on ice with 1 x cell lysis buffer (Cell Signaling) containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed 3 times for 1 minute then incubated with a secondary antibody polymer (Cell Signaling-Signal Stain boost ...
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bioRxiv - Developmental Biology 2023Quote: ... The membrane was washed three times with a 1X Tris Buffered Saline (TBS) (Cell Signaling Technology, 12798) and Tween 20 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... bots were washed three times using TBST and incubated with secondary antibodies (Cell Signaling: #7074 and #7076). The blots were then visualized and imaged using Pierce ECL Western Blotting Substrate (Thermo Fisher 32106 ...
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bioRxiv - Molecular Biology 2021Quote: Cell proliferation was examined using DNA synthesis analysis (bromodeoxyuridine (BrdU) incorporation assay) or Ki67 detection with specific antibody (Cell Signaling Technology, Danvers, MA) as described previously (7 ...
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bioRxiv - Neuroscience 2019Quote: DREADDs animal brain sections were incubated overnight at room temperature in rabbit anti-HA (for detection of HA-Tag; 1:500; Cell Signaling Technology, MA) and mouse anti–dopamine beta hydroxylase (DBH ...
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bioRxiv - Biochemistry 2021Quote: ... and followed with detection by Neutravidin-DyLight-800 (Pierce 22853) for biotin-ADP ribosylated Ub and anti-Ub-H2A (Cell signaling Technology #8240) for H2A ubiquitylation.
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bioRxiv - Cancer Biology 2023Quote: ... sections were incubated (for 1 h) at room temperature with SignalStain® Boost Detection Reagent (HRP, cat. no. #8114; Cell Signaling Technologies, Inc.). Sections were washed three times with wash buffer and developed with freshly prepared and filtered 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Microbiology 2023Quote: ... expression of hACE and cACE2 was validated via immunofluorescence and immunoblots through detection of the c-terminal myc tag with a 1:500 dilution of antibody Myc-Tag-9B11 Mouse mAb (Cell Signaling Technology, 2276).
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bioRxiv - Immunology 2022Quote: ... the membrane was incubated for one hour at RT with peroxidase-conjugated goat anti-rabbit secondary antibody (Cell Signaling Technology, #7074, 1:1000) and washed again ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane was blotted with 5% nonfat milk in TBST at RT for 1h and then incubated with the following antibodies at 4°C overnight: TWIST1(Cell Signaling Technology, 46702S), E-cadherin (Cell Signaling Technology ...
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bioRxiv - Immunology 2020Quote: ... membranes were incubated with primary antibodies for 1h at RT with shaking (1:2000 anti-filaggrin clone Poly19058, Biolegend; 1:2000 anti-β-actin, clone 13E5, Cell Signaling Technology). After washing (3×5 min in Tris-buffered saline containing 0.1% v/v Tween20 ...
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bioRxiv - Physiology 2022Quote: ... for 1 hour at RT and then incubated O/N with primary antibody for c-Fos (Rabbit anti-cFos, Cell Signaling, Cat# 2250) in blocking solution at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were blocked with TBST/5% Normal Goat Serum for 1 hour at RT before overnight incubation with primary antibody diluted in Diluent CST (Cell Signaling Technology, 8112) at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... followed by anti-rabbit IgG HRP linked secondary antibody at 1:10,000 in TBS-T for 1h at RT (antibodies were from Cell Signaling Technology, MA, USA). Protein bands were detected by exposing the membrane to SignalFire™ ECL substrate (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... permeabilized cells were blocked for 30 minutes at room temperature (RT) in 5% (v/v) normal goat serum (NGS) (Cell Signaling Technology, #5425) DPBS solution ...
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bioRxiv - Cell Biology 2024Quote: ... cells were permeabilized in 0.25% Triton X-100 in PBS for 5 minutes and blocked in 10% goat serum/0.1% Triton X-100 in PBS for 30 minutes and then placed in the primary antibody overnight at RT (γH2AX, Cell Signaling, 1:500). The next morning ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed 3 times in tris-buffered saline with 0.1% Tween 20 (TBS-T, Cell Signaling, 9997) at room temperature and subsequently incubated with secondary antibodies for 1 hour at room temperature in Odyssey blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and washed again three times for 10 min in TBST before exposure using ECL (SignalFire Plus, Cell signaling). The following antibodies and concentrations were used ...
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bioRxiv - Bioengineering 2022Quote: ... washed 3 times with 1X PBS and incubated with the following antibodies Phospho-YAP (S127) (Cell Signaling 13008), YAP (Cell Signaling 4912 ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were washed 3 times in TTBS and incubated with anti-mouse-HRP (Cell Signaling, 7076S; 1:10,000) in 5% BSA for 1 hr at RT.
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bioRxiv - Biochemistry 2020Quote: ... washed with 1x TBST solution three times and then incubated with Rabbit IgG-HRP antibody (Cell Signaling, #7074S) for 1□h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed three times and resuspended in FACS buffer containing 0.2 µg/mL DAPI (Cell Signaling Technology). For samples that could not be analyzed on the day of staining and required fixation ...
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bioRxiv - Cancer Biology 2023Quote: ... before the slides were washed three times with PBS and mounted using antifade mounting media (Cell Signaling 9071). Images were captured at 60x magnification using a Nikon ECLIPSE Ti2 microscope ...
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bioRxiv - Physiology 2024Quote: ... Membranes were then washed 3 times in Tris-buffered saline containing 0.1% Tween-20 (TBST, Cell Signaling Technology) prior to 1-hr blocking with blocking buffer (5% Blotting Grade Blocker (Bio Rad ...
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bioRxiv - Microbiology 2020Quote: ... for evaluation of histopathologic changes by H&E staining or for immunohistochemical detection of reovirus antigen (1:45,000 dilution) or the cleaved (active) form of caspase-3 (Cell Signaling, 9664; 1:300 dilution). Images were captured at a magnification of ×20 and a resolution of 0.5 μm/pixel using a high-throughput Leica SCN400 slide scanner automated digital imaging system.
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bioRxiv - Neuroscience 2022Quote: ... Antigen detection was performed by overnight incubation with appropriate antibodies: 1:1000 rabbit monoclonal Plexin-A4 (catalog #C5D1) anti-mouse (Cell Signaling Technology, catalog #3816), 1:1000 rabbit monoclonal Nrp1 (catalog #D62C6 ...
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bioRxiv - Microbiology 2021Quote: ... Immunoblot detection of phosphorylated paxillin was performed using a 1:1000 dilution of a mouse anti-phospho-paxillin (Y-118) (Cell Signaling Technology, Danvers, MA) antibody ...
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bioRxiv - Microbiology 2021Quote: ... Immunoblot detection of phosphorylated paxillin was performed using a 1:2000 dilution of a mouse anti-phospho-paxillin (Y-118) (Cell Signaling Technology, Danvers, MA) antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween for 1 h and detection was carried out overnight at 4 °C with rabbit anti-phospho-Chk1 (Ser345) mAb (133D3) (Cell Signaling 2348; Lot 15) or rabbit anti-Phospho-Chk2 (Thr68 ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed with horseradish peroxidase-conjugated antibodies directed against rabbit or mouse IgG (HRP-linked α-mouse IgG (Cell Signaling Technology, Cat. 7076), HRP-linked α-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were then incubated for 2 hours at room temperature (RT) in antibody binding buffer (PBSTr +1% (v/v) DMSO) before overnight incubation with 1:1000 α-pSmad2 antibody (Cell Signaling Technology #18338) and ...
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bioRxiv - Cell Biology 2021Quote: ... then incubated for 1.5 h at RT with either CD36 (Novus NB400-144, 1:500 dilution) or PPARγ (Cell Signaling 2430S, 1:250 dilution) and GAPDH (Abcam ab181602 ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween and detection of total CHK1 or CHK2 proteins was carried out for 1 h at RT using mouse-anti-Chk1 mAb (2G1D5) (Cell Signaling 2360; Lot 3) or mouse anti-Chk2 mouse mAb (BD Biosciences 611570) ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were stained with Ponceau S solution and blocked with 5% nonfat milk in PBS/0.05% Tween 20 for 1 h at RT and subsequently incubated at 4 °C overnight with the following primary antibodies: rabbit anti-MBP (1:1000; Cell Signaling, Danvers, MA, USA), mouse anti-CRYAB (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... fixed with 4 % paraformaldehyde/1xPBS for 10 min at RT and solubilized with 0.1% Triton X-100 /1xPBS before staining with antibodies against LC3A/B (Cell Signaling Technologies, cat no. 12741S) and SQSTM1 (Cell Signaling Technologies ...
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bioRxiv - Biophysics 2023Quote: ... and incubated for 1h at RT with peroxidase-conjugated secondary antibodies (anti-mouse IgG, Cell Signaling, cat. 7076; anti-rabbit IgG, Cell Signaling, cat. 7074) diluted 1:5000 in 5% non-fat dried milk in TBS-T solution ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The NC membrane was incubated for 1 hour at RT in blocking buffer (5% nonfat milk in PBS [pH 7.4] containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 hours at RT with antibodies to CFTR (1:1000, Cell signaling Technology Cat. No. 78335,), SNAP-23 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies: anti-Glucocorticoid Receptor D6H2L (1:1000 dilution, Cell Signaling, New England BioLabs #12041), anti-PAX7c (1:500 dilution ...
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bioRxiv - Immunology 2023Quote: ... with WESTM equipment, with detection of TSC2 (Cell Signalling Technology, Tuberin/TSC2, catalogue # D93F12, XP® Rabbit mAb) and GAPDH (14C10, Cell Signaling Technology catalogue # 2118). TSC2 expression was normalized to GAPDH via signal peak area.