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Citations for Cell Signaling Technology :
201 - 250 of 762 citations for hsa mir 373 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... for 1 hour at RT and then incubated O/N with primary antibody for c-Fos (Rabbit anti-cFos, Cell Signaling, Cat# 2250) in blocking solution at RT ...
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bioRxiv - Bioengineering 2023Quote: ... followed by anti-rabbit IgG HRP linked secondary antibody at 1:10,000 in TBS-T for 1h at RT (antibodies were from Cell Signaling Technology, MA, USA). Protein bands were detected by exposing the membrane to SignalFire™ ECL substrate (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were blocked with TBST/5% Normal Goat Serum for 1 hour at RT before overnight incubation with primary antibody diluted in Diluent CST (Cell Signaling Technology, 8112) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were permeabilized in 0.25% Triton X-100 in PBS for 5 minutes and blocked in 10% goat serum/0.1% Triton X-100 in PBS for 30 minutes and then placed in the primary antibody overnight at RT (γH2AX, Cell Signaling, 1:500). The next morning ...
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bioRxiv - Molecular Biology 2024Quote: ... permeabilized cells were blocked for 30 minutes at room temperature (RT) in 5% (v/v) normal goat serum (NGS) (Cell Signaling Technology, #5425) DPBS solution ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed 3 times in tris-buffered saline with 0.1% Tween 20 (TBS-T, Cell Signaling, 9997) at room temperature and subsequently incubated with secondary antibodies for 1 hour at room temperature in Odyssey blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and washed again three times for 10 min in TBST before exposure using ECL (SignalFire Plus, Cell signaling). The following antibodies and concentrations were used ...
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bioRxiv - Bioengineering 2022Quote: ... washed 3 times with 1X PBS and incubated with the following antibodies Phospho-YAP (S127) (Cell Signaling 13008), YAP (Cell Signaling 4912 ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were washed 3 times in TTBS and incubated with anti-mouse-HRP (Cell Signaling, 7076S; 1:10,000) in 5% BSA for 1 hr at RT.
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bioRxiv - Biochemistry 2020Quote: ... washed with 1x TBST solution three times and then incubated with Rabbit IgG-HRP antibody (Cell Signaling, #7074S) for 1□h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed three times and resuspended in FACS buffer containing 0.2 µg/mL DAPI (Cell Signaling Technology). For samples that could not be analyzed on the day of staining and required fixation ...
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bioRxiv - Cancer Biology 2023Quote: ... before the slides were washed three times with PBS and mounted using antifade mounting media (Cell Signaling 9071). Images were captured at 60x magnification using a Nikon ECLIPSE Ti2 microscope ...
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bioRxiv - Physiology 2024Quote: ... Membranes were then washed 3 times in Tris-buffered saline containing 0.1% Tween-20 (TBST, Cell Signaling Technology) prior to 1-hr blocking with blocking buffer (5% Blotting Grade Blocker (Bio Rad ...
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bioRxiv - Microbiology 2020Quote: ... for evaluation of histopathologic changes by H&E staining or for immunohistochemical detection of reovirus antigen (1:45,000 dilution) or the cleaved (active) form of caspase-3 (Cell Signaling, 9664; 1:300 dilution). Images were captured at a magnification of ×20 and a resolution of 0.5 μm/pixel using a high-throughput Leica SCN400 slide scanner automated digital imaging system.
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bioRxiv - Neuroscience 2022Quote: ... Antigen detection was performed by overnight incubation with appropriate antibodies: 1:1000 rabbit monoclonal Plexin-A4 (catalog #C5D1) anti-mouse (Cell Signaling Technology, catalog #3816), 1:1000 rabbit monoclonal Nrp1 (catalog #D62C6 ...
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bioRxiv - Microbiology 2021Quote: ... Immunoblot detection of phosphorylated paxillin was performed using a 1:1000 dilution of a mouse anti-phospho-paxillin (Y-118) (Cell Signaling Technology, Danvers, MA) antibody ...
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bioRxiv - Microbiology 2021Quote: ... Immunoblot detection of phosphorylated paxillin was performed using a 1:2000 dilution of a mouse anti-phospho-paxillin (Y-118) (Cell Signaling Technology, Danvers, MA) antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween for 1 h and detection was carried out overnight at 4 °C with rabbit anti-phospho-Chk1 (Ser345) mAb (133D3) (Cell Signaling 2348; Lot 15) or rabbit anti-Phospho-Chk2 (Thr68 ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed with horseradish peroxidase-conjugated antibodies directed against rabbit or mouse IgG (HRP-linked α-mouse IgG (Cell Signaling Technology, Cat. 7076), HRP-linked α-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were then incubated for 2 hours at room temperature (RT) in antibody binding buffer (PBSTr +1% (v/v) DMSO) before overnight incubation with 1:1000 α-pSmad2 antibody (Cell Signaling Technology #18338) and ...
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bioRxiv - Cell Biology 2021Quote: ... then incubated for 1.5 h at RT with either CD36 (Novus NB400-144, 1:500 dilution) or PPARγ (Cell Signaling 2430S, 1:250 dilution) and GAPDH (Abcam ab181602 ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween and detection of total CHK1 or CHK2 proteins was carried out for 1 h at RT using mouse-anti-Chk1 mAb (2G1D5) (Cell Signaling 2360; Lot 3) or mouse anti-Chk2 mouse mAb (BD Biosciences 611570) ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were stained with Ponceau S solution and blocked with 5% nonfat milk in PBS/0.05% Tween 20 for 1 h at RT and subsequently incubated at 4 °C overnight with the following primary antibodies: rabbit anti-MBP (1:1000; Cell Signaling, Danvers, MA, USA), mouse anti-CRYAB (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... fixed with 4 % paraformaldehyde/1xPBS for 10 min at RT and solubilized with 0.1% Triton X-100 /1xPBS before staining with antibodies against LC3A/B (Cell Signaling Technologies, cat no. 12741S) and SQSTM1 (Cell Signaling Technologies ...
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bioRxiv - Biophysics 2023Quote: ... and incubated for 1h at RT with peroxidase-conjugated secondary antibodies (anti-mouse IgG, Cell Signaling, cat. 7076; anti-rabbit IgG, Cell Signaling, cat. 7074) diluted 1:5000 in 5% non-fat dried milk in TBS-T solution ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The NC membrane was incubated for 1 hour at RT in blocking buffer (5% nonfat milk in PBS [pH 7.4] containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 hours at RT with antibodies to CFTR (1:1000, Cell signaling Technology Cat. No. 78335,), SNAP-23 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies: anti-Glucocorticoid Receptor D6H2L (1:1000 dilution, Cell Signaling, New England BioLabs #12041), anti-PAX7c (1:500 dilution ...
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bioRxiv - Immunology 2023Quote: ... with WESTM equipment, with detection of TSC2 (Cell Signalling Technology, Tuberin/TSC2, catalogue # D93F12, XP® Rabbit mAb) and GAPDH (14C10, Cell Signaling Technology catalogue # 2118). TSC2 expression was normalized to GAPDH via signal peak area.
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed three times in PBS and mounted with Prolong Gold anti-fade reagent with DAPI (Cell Signaling).
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bioRxiv - Cell Biology 2021Quote: ... samples were washed 3 times with PBS and then incubated with secondary anti-Rabbit-AlexaFluor488 (Cell Signaling Technology, 4412) and anti-mCherry-AlexaFluor594 (Invitrogen #M11240 ...
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bioRxiv - Molecular Biology 2023Quote: ... washed three times with cold PBS and lysed in 1 ml of 1x Cell Lysis Buffer (Cell Signaling, #9803) in the presence of a protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... Aβ deposits were detected using primary anti-body specific to isoforms Aβ 37-42 (Aβ D54D2 XP, Cell Signaling Technology, 1:100 dilution, overnight at RT) and further visualized by fluorescent Alexa 568 secondary antibody (1:500 dilution ...
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bioRxiv - Neuroscience 2021Quote: Brains were homogenized using Qiagen Tissue-Lyser II (28 Hz, 3 min, 3 times) in lysis buffer (Cell Signaling #9803) containing protease inhibitor cocktail (Roche #4693159001 ...
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bioRxiv - Microbiology 2020Quote: ... washed three time with PBS and stained with primary antibody (α-IFITM1 Cell Signaling 13126 S, α-IFITM2 Cell Signaling 13530S ...
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bioRxiv - Microbiology 2022Quote: ... washed three times in TBST and incubated with horseradish peroxidase-conjugated second antibody from Cell Signaling Technology (dilution 1:2000) for 1 h at RT ...
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bioRxiv - Immunology 2022Quote: ... Membranes were washed several times with TBST before incubating with a horseradish peroxidase-conjugated anti-rabbit secondary antiserum (1:4,000, Cell Signaling) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... was applied and the sections were washed 3 times and were detected using the DAB method (#80259P, Cell Signaling Technology). Standard hematoxylin and eosin staining was performed on rehydrated fat paraffin sections ...
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bioRxiv - Cell Biology 2022Quote: ... washed three times with PBS and blocked for 1h in PBS containing 5% normal goat serum (Cell signaling, Cat # #5425) and 0.3% Triton X-100 ...
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bioRxiv - Molecular Biology 2024Quote: ... beads were washed three times in 1 mL Lysis Buffer and twice in 1 mL 1x Kinase Buffer (Cell Signaling) supplemented with Phosphatase Inhibitors (PhosSTOP ...
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bioRxiv - Neuroscience 2023Quote: ... Membrane were washed three times with T-TBS then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... Then membranes were incubated for 1 h at RT with secondary anti-mouse or anti-rabbit HRP-linked antibodies (Cell Signaling Technology #7076 or # 7074; 1:5000) diluted in Bullet Blocking One and were washed three times for 15 min in 1×TBST buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... The membrane was washed 3 times in TBST and incubated for 1 hour at room temperature with HRP-conjugated anti-rabbit secondary antibody (Cell Signaling) diluted 1:1000 in blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times with TBS-T (0.05% v/v Tween-20 in TBS) before incubation with HRP conjugated antibody (Cell Signaling Technology 7076S ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then washed three times with PBS and total cells were labeled with a 1:1,000 dilution of DRAQ5 (Cell Signaling Technology) for 5 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed in PBS-0,1% Tween20 three times for 15 min at room temperature and anti-rabbit or anti-mouse secondary antibodies (1:3000, Cell signaling) were applied for 30 min at room temperature with gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were washed with tris-buffered saline with Tween 20 (TBST) three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed three times with TBS containing 0.1% Tween 20 then incubated with anti-Rabbit IgG-HRP conjugated (Cell Signaling: 7074). Images were acquired by chemiluminescence using Clarity ECL (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then washed three times with PBS and total cells were labeled with a 1:1,000 dilution of DRAQ5 (Cell Signaling Technology) for 5 min at 4°C ...