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Citations for Cell Signaling Technology :
1 - 50 of 559 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Protein expression was normalized to α-TUBULIN expression (Cell Signaling) using the ImageLab 4.1 software (Bio-Rad).
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bioRxiv - Cancer Biology 2019Quote: ... Protein expression was analyzed by immunoblotting for expression of phospho-EGFR (Y1068, 1:1000, Cell Signaling, Danvers, MA, USA), total EGFR (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Effective expression was confirmed by Western blot (SCN1B: Cell Signaling #13950S ...
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was checked using antibodies against Akt (Cell Signaling Technology). Insulin-stimulated glucose uptake was measured using the Glucose Uptake-Glo assay kit (Promega ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast were incubated with a 1:100 dilution of AF647-conjugated anti-c-Myc antibody (clone 9B11, Cell Signaling Technologies) in PBE for 2 hr at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The expression of vinculin (rabbit anti-vinculin; 1:1000; 4650S; Cell Signaling) was used as a loading control.
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bioRxiv - Cancer Biology 2023Quote: ... and Cas9 expression was confirmed by Western Blot (Cell Signaling Technology #14697). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Expression of pluripotency markers was analyzed by flow cytometry using SOX2 (Cell Signaling) and OCT3/4 (Santacruz Biotechnology ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-HA to detect hM3Dq expression (Cell Signaling Technologies, 3724S, 1:500), donkey anti-rabbit Alexa Fluor 488 (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Expression of pluripotency marker NANOG was analyzed by immunofluorescence using antibodies from Cell Signaling.
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bioRxiv - Cell Biology 2020Quote: ... Following antibodies were used to detect protein expression: anti phosphorylated ERKs (Cell Signaling Technology), anti total ERKs (Santa Cruz Biotechnology) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... for FPR2 expression analysis or and goat-anti-rabbit IgG HRP (Cell Signaling Technology) were used as primary and respective secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... The expression was further confirmed by immunoblot using hACE2 Ab (Cell Signaling Technology, # 4355). The open reading frame of hACE2 (kindly provided by Sonja Best from NIAID/NIH ...
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bioRxiv - Microbiology 2020Quote: ... FLAG expression was detected using a monoclonal rabbit anti-FLAG antibody (Cell Signaling Technology, #14793S) and a goat anti-rabbit AlexaFluor-594 secondary antibody (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies used to detect protein expression levels are as follows: caspase-9 (Cell Signaling: 9502), Bim (Abcam:32158) ...
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bioRxiv - Physiology 2023Quote: ... CD44 expression and nuclear localization was determined using anti-CD44 (Cell Signaling; Catalogue number: 3570S) and PECAM-1 expression using anti-PECAM-1 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Homogeneous dCas9-KRAB expression was confirmed by intracellular staining using Cas9 antibody (Cell Signaling Cat-14697) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: The CAR expression level was quantified using myc tag-FITC staining (Cell Signaling Technology, Danvers, MA). T cell subtypes were determined by staining for CD4-PE/Cy7 and CD8-eF450 (BD Biosciences ...
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bioRxiv - Genetics 2021Quote: ... The expression of NQO1 protein were detected using NQO1 monoclonal antibody (Cell signaling technologies, Beverly, MA). After post-primary blocking the sections were incubated with Novolink polymer and were then developed with DAB using supplied substrate buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... and signal (protein expression) was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Cell Signaling, 1:1000).
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bioRxiv - Neuroscience 2023Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367 ...
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bioRxiv - Cancer Biology 2024Quote: ... and confirmed for KRASG12C expression by Western blotting using an HA tag antibody (Cell Signaling Technology).
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bioRxiv - Cancer Biology 2023Quote: ... CARs expression on CAR T cells was assessed with PE-MycTag (1:100; Cell Signaling, #64025S). CD276 CARs expression on CAR T cells was assessed with human B7H3-Fc chimeric protein (20µg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... and radixin expression was confirmed by immunoblotting using the monoclonal C4G7 antibody (Cat# 2636S, Cell Signaling). Negative control cell lines ...
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bioRxiv - Plant Biology 2023Quote: ... Hemagglutinin (HA)-tagged mutant PpRopGAP1 in yeasts was detected with an anti-HA primary antibody (C29F4) of rabbit origin (Cell Signaling Technology, cat. 3724T, 5000× dilution) and an anti-rabbit-HRP (goat ...
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bioRxiv - Neuroscience 2021Quote: ... Protein expression was normalized to the internal control β-actin (1:100; CST #4970, Cell Signaling Technologies). In order to generate comparable data for all samples ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were collected from clones and western blots were performed to determine Cas9 expression (Cell Signaling #14697). Cas9 editing efficiency was determined by viability studies using sgRNAs targeting selected fitness genes (PSMD1 ...
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bioRxiv - Neuroscience 2021Quote: iPSC clones were initially tested for pluripotency by assessing expression of pluripotency markers Oct4 (Cell Signaling, # C30A3), SSEA3/4 (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... for determination of the p53 expression level and mouse monoclonal anti-p53(Ser15)/16G8 (9286) (Cell Signaling) for determination of the p53 Ser15 phosphorylation level ...
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bioRxiv - Microbiology 2023Quote: ... The expression of ATG16L1 was determined by Western blotting using an anti-ATG16L1 antibody (Cell Signaling Technology).
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bioRxiv - Cancer Biology 2023Quote: ... We examined cell proliferation in the carcinogen-induced tumors by examining Ki67 expression (Cell Signaling, Cat #12202S), CD47 expression within a matched human primary and metastatic tumor tissue array (Anti-human CD47 purified (Clone B6H12) ...
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bioRxiv - Cancer Biology 2024Quote: ... and expression of the construct was confirmed by WB with an HA tag antibody (Cell Signaling Technology).
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bioRxiv - Physiology 2024Quote: ... Data were normalized for the expression of the housekeeping protein β-actin (cs4967, Cell Signaling, 1:5000).
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bioRxiv - Bioengineering 2021Quote: ... MAP2 expression was detected by immunofluorescent staining using anti-MAP2 antibodies (Cell Signaling Technology Inc., Danvers, MA, USA). The cells used for the following experiments were aged between 6 and 9 days of culture.
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bioRxiv - Microbiology 2020Quote: ... FLAG expression was detected using a monoclonal mouse anti-FLAG antibody (Cell Signaling Technology, Danvers, MA, USA; #8146S). Quantification of band intensity was performed using Fiji ImageJ.
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bioRxiv - Microbiology 2023Quote: ... Expression of iKd PfGEXP15 protein was checked by Western blotting using anti-HA (1/1000, Cell signaling C29F4) followed by anti-Rabbit IgG (1/20000 ...
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bioRxiv - Cell Biology 2024Quote: ... The absence of ezrin expression was confirmed through immunoblotting with an anti-ezrin antibody (Cat# 3145S, Cell Signaling). Triple ERM knockouts were generated using the radixin target region sequence was 5’-AAAACAGTTGGCTTACGTG’ ...
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bioRxiv - Cancer Biology 2022Quote: ... Full loss of PTEN expression was finally validated by Western blotting (anti-PTEN antibody, Cell Signaling, 9559S, 1:1000). sgRNAs come from Cederquist et al.57:
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bioRxiv - Cancer Biology 2023Quote: ... DDX3X expression was checked by western blotting using an anti-DDX3X antibody (D19B4, Cell Signaling Technology, Danvers, MA, USA).
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bioRxiv - Cell Biology 2024Quote: ... and the absence of moesin expression was verified by immunoblotting using the Q480 monoclonal antibody (Cat# 3150S, Cell Signaling). To generate double EM knockouts ...
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bioRxiv - Microbiology 2023Quote: ... ISG15 expression was evaluated using SimpleWestern Abby (ProteinSimple, San Jose, CA, USA) with anti-ISG15 rabbit polyclonal antibody (Cell Signaling Biotechnology ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... and screened for Cas13d expression using western blotting and immunofluorescence methods with an anti-HA antibody (Cell Signaling Cat #2367). Cas13 knockdown activity was confirmed by FACS using CD46 as a positive control (Cell Signaling Cat #13241).
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bioRxiv - Molecular Biology 2021Quote: ... Expression of Anti-HA fusion protein was examined by staining cells with rabbit Anti-HA (1:250; #3724, Cell Signaling Technology) and Alexa Fluor@ 488 donkey anti-rabbit IgG (1:2000 ...
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bioRxiv - Molecular Biology 2019Quote: ATM protein expression was assessed by SDS-PAGE and western blot using a rabbit anti-ATM antibody (1:1000; 2873S; Cell Signaling). Horseradish peroxidase-conjugated α-rabbit antibody (1:5000 ...
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bioRxiv - Cell Biology 2020Quote: Protein expression levels of PANX1 were examined by western blotting using a rabbit monoclonal antibody against human PANX1 (D9M1C, Cell Signaling). Actin was also detected as a control by western blotting using a mouse monoclonal anti-β-actin antibody (Santa Cruz) ...
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bioRxiv - Cancer Biology 2022Quote: ... Expanded clones were tested for Rb expression via Western blotting (antibody clones listed in Table S1, Cell Signaling Technologies, Danvers, MA). For in vitro drug treatments ...
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bioRxiv - Cancer Biology 2022Quote: Cells with stable expression of FLAG-tagged human PDE6D were lysed in 1X cell lysis buffer (#9803, Cell Signaling, Danvers, MA) and incubated with anti-FLAG M2 Affinity Gel (A2220 ...
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bioRxiv - Microbiology 2022Quote: ... we stimulated NFκB-dependent gene expression by treating cells with purified human TNF (100 ng per ml; Cell Signaling Technology, Inc.) in 1X Opti-MEM reduced serum media (Gibco Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... TMPRSS2 expression was evaluated using SimpleWestern Abby (ProteinSimple, San Jose, CA, USA) with an anti-Myc tag mouse monoclonal antibody (Cell Signaling Biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was used to check the expression level of CBP and P300 by Western blot using anti-P300 and anti-CBP antibodies (Cell Signaling). Anti–β-tubulin antibody (Hybridoma bank # AB_2315513 ...