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Citations for Cell Signaling Technology :
101 - 150 of 169 citations for West Nile virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The lysate samples were denatured by mixing with Blue Loading Gel Dye and DTT (Cell Signaling Technology, 7722). Primary antibodies used in this experiment were as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were prepared from control and treated cells in cell lysis buffer (Cell Signaling, Danvers, MA, USA) with protease and phosphatase inhibitors (Halt Protease and Phosphatase Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... The rest of the lysate was split in half and either 1:100 Oct4 antibody (Cell Signaling, 2750) or rabbit IgG isotype control (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: Whole-cell lysates were prepared using cell lysis buffer and sonication as described in standard protocols (Cell Signaling). Immunoprecipitations were performed using NOTCH3 or MAML1 antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... each lysate was pre-cleared with 20 μL of pre-washed Protein A agarose beads (Cell Signaling 9863S). Next ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... Lysates were probed using anti-β-actin and anti-GAPDH (1:1000 dilution, Cell Signaling Technology, # 4967, # 97166). Either anti-rabbit-680 or anti-mouse-800 (1:5000 dilution ...
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bioRxiv - Genomics 2020Quote: ... was added to sonicated lysate along with 0.48 μg of c-Jun antibody (cat. no. 9165; Cell Signaling Technology) or equal amount of rabbit IgG (cat ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μg of nuclear protein lysates were incubated with 2 μg of either Myc-Tag antibody (Cell Signaling Technology) or an endogenous NUP210-specific antibody (Bethyl Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: ... Lysate was incubated for 4 h at 4°C with anti-ECSIT2 antibody39 or IgG control (Cell Signaling, #2729), followed by overnight precipitation with 30 µL of washed Protein A sepharose 4B beads (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were prepared using RIPA buffer containing cOmplete ULTRA protease and phosphatase inhibitor (Cell Signaling Technology (CST), Danvers ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were incubated overnight at 4°C with anti-Myc-sepharose made with anti-Myc antibody (Cell signaling) and Pierce Crosslink IP Kit (Thermo/Pierce) ...
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bioRxiv - Physiology 2022Quote: ... Protein lysates were incubated overnight at 4 °C with rabbit acetyl-lysine (Ac-K, #9441) from Cell Signaling Technology. Protein densitometry was measured using Image J software (National Institutes of Health ...
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bioRxiv - Pathology 2021Quote: Tissue lysates were generated using 1 mL per 100 mg tissue in lysis buffer (Cell signaling Technologies, Danvers, MA). Total protein contents were quantified using a Pierce BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Physiology 2019Quote: ... lysates were ran out on 4-12% gradient gels and probed with anti-phospho(T389) p70S6K (Cell Signaling 9205) and anti-p70S6K (Cell Signaling 2708 ...
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bioRxiv - Cancer Biology 2022Quote: ... the lysates were incubated at 4°C for 5 h with antibodies for BRD4 (Cell Signaling Technology; 1:100). Antibody/antigen complexes were recovered using Dynabeads Protein A/G (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... Protein lysates were incubated overnight at 4°C with mouse α-Myc antibody (1:400, [Cell Signaling Technologies, #2276]) on a rotisserie tube rotator ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... the sonicated lysate was then separated to a new tube and primary antibodies was added (anti-H2AK119Ub Cell Signaling D27C ...
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bioRxiv - Immunology 2023Quote: ... and the remaining lysate was incubated with Phospho-AMPK Substrate Motif mAb mix (Cell Signaling Technologies; catalog No. 5759) for 12 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were then incubated overnight (12 h) at 4 °C with mouse anti–NCS-1 (1:500; Cell Signaling). Samples were subsequently incubated for 2 h with Protein-G–Sepharose (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were incubated with 10 ug of anti-Nup98 antibody or an irrelevant isotype control (IgG DA1E, Cell signaling) overnight and subsequently incubated with protein G-beads for 2h ...
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bioRxiv - Cancer Biology 2019Quote: ... lysates were immunoblotted with use of an anti-Brn2 antibody (Santa Cruz Biotechnology, sc-393324 or Cell Signaling Technology, #12137), anti-p63 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... and lysates were then incubated overnight with primary anti-LRRC23 or Rabbit IgG (#2729, Cell Signaling Technology, Danvers, Massachusetts, USA) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal protein concentration were incubated overnight in 4 °C with an anti-Src antibody (Cell Signaling Technology, #2108). Complexes were pulled out using protein-A sepharose beads (GE-Healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: ... Up to 30 µg of protein lysate was separated by SDS-PAGE and transferred to nitrocellulose membranes before antibody incubation (IκBα: Cell Signaling, CS9242 ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were sacrificed 6 days after noise exposure and whole cochlear lysates were prepared in lysis buffer (9803; Cell Signaling) after adding protease (cOmplete ULTRA Tablets 05892791001 ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysate was obtained by incubating treated and untreated DLD-1 cells in 1X RIPA (Cell Signaling Tech, Beverly MA), with phosphatase inhibitor cocktail and protease inhibitor tablets (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... The bead-immobilized GTP-bound RhoA and total RhoA in the lysates were probed by immunoblotting with anti-RhoA antibody (Cell Signaling).
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bioRxiv - Biochemistry 2020Quote: IP was performed on hepatocytes lysates using antibodies against LxRxx[S*/T*] and Rxx[S*/T*] phospho-motifs (both Cell Signaling) with Pierce Protein A/G Magnetic Beads according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were recovered following centrifugation and incubated overnight with 50 μL Streptavidin magnetic beads (Cell Signaling Technology, Danvers, MA, USA). Beads were rinsed using a magnetic stand at 25°C according to the following steps ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates for protein analysis were prepared by scraping cells in 1x Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA, USA) containing 1x protease/phosphatase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... For immunoprecipitation assays, cell lysates were incubated with Protein G Agarose (cat # 20398, Pierce) and anti-Flag antibody (cat # 2368, Cell Signaling) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Microbiology 2020Quote: ... 250 μL of lysate were incubated with 2 μL of mouse Myc-Tag antibody clone 9B11 (Cell Signaling Technology, Cat. 2276S) for 4 h with rotation at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Antibodies for detection of cell surface proteins by western blot of cell lysates were used as provided and were from the following sources: anti-ErbB3 (Cell Signaling, HER3/ErbB3 ...
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bioRxiv - Microbiology 2023Quote: ... The efficiency of siRNA knockdown was verified by immunoblotting of whole cell lysates with an anti-E-cadherin (24E10, Cell signaling) antibody.
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bioRxiv - Neuroscience 2023Quote: ... Lysates were co-imunoprecipitated with anti-HA beads (HA-Tag C29F4 Rabbit mAb Sepharose® Bead Conjugate Cell Signaling #3956 RRID:AB_10695091) overnight at 4 °C under gentle agitation ...
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bioRxiv - Developmental Biology 2023Quote: ... aliquots of total cell lysate were subjected to SDS-PAGE and Western blot analysis with anti-phosphotyrosine (1:3000, Cell signaling) antibody ...
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bioRxiv - Microbiology 2023Quote: ... the cell lysate was subjected to SDS-PAGE and western blot analyses using anti-HA antibody (1:2,000, 2999S, Cell Signaling Technology).
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bioRxiv - Biochemistry 2024Quote: ... 10 mg of lysate representing one biological replicate was incubated for 2 hours at 4 °C with anti-Kv11.1 antibody (Cell Signaling #12889) covalently coupled to protein A/G magnetic beads (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... 5mg of lysate was incubated with 30ul of MyOne Streptavidin C1 Dynabeads or 5mg of Myc-Tag (9B11) Mouse mAb (Cell Signaling, #2276) antibody for 3 hours with subsequent 1-hour incubation with 30ul of Protein G Dynabeads for streptavidin pulldown and myc tag IP ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were first titrated on CD34+ fetal liver lysates and subsequently used at the following dilutions: Anti-GATA1 (Cell Signaling, 1:5), anti-STAG2 (Cell Signaling ...
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bioRxiv - Biophysics 2022Quote: ... Cellular protein tyrosine phosphorylation was performed by immunoblotting of total cell lysate (20 μg) using anti-pTyr antibody (4G10, a gift form Prof. Mangeat; CRBM) and anti-Actin (Cell Signaling Technology). Signal quantification and analyses were performed using ImageJ and GraphPad Prism respectively ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μl cell lysates served as a source of the E3 ligase CHIP (cells were lysed in Cell lysis buffer (9803, Cell Signaling Technology). After reactions ...
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bioRxiv - Genomics 2022Quote: ... acid-extracted histone lysate was prepared accordingly (Shechter et al., 2007) and subjected to western blotting using anti-H3K9ac (Cell Signaling, 9649), H3K14ac (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... the same amount of each cellular lysate was analyzed by gel electrophoresis and western blot using anti-WDR5 antibody (D9E1I; Cell Signaling #13105), anti-HSP90 antibody (F-8 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were immunoprecipitated from precleared lysates overnight at 4°C using 4 μg of control IgG (rabbit or mouse, Cell Signaling Technology) or indicated antibody ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were lysed in ODG buffer and the lysates were incubated with an anti-Myc-tag antibody (2276, Cell Signaling Technology). Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... the sonicated lysate was then separated to a new tube and primary antibodies was added (anti-H2AK119Ub Cell Signaling D27C, anti-H3K4me3 Cell Signaling CD42D8 ...