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Citations for Cell Signaling Technology :
401 - 450 of 8139 citations for Tripartite Motif Containing Protein 55 TRIM55 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... To detect phosphorylated ERM proteins a rabbit anti-phospho–ezrin (Thr567)/radixin (Thr564)/moesin (Thr558) (pERM) antibody was used (Cell Signaling Technology). Isotype control staining was performed using rabbit IgG isotype mAb Alexa Fluor 647 (DA1E ...
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bioRxiv - Microbiology 2023Quote: ... The spike protein and fragments were detected by rabbit monoclonal antibodies (mAb) against the 6-His tag (12698S) from Cell Signaling Technology. The nitrocellulose (NC ...
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated proteins were detected by probing the membranes with an anti-phospho-c-Met antibody (Tyr1234/1235, #3077, Cell Signaling Technology) or an anti-phospho-EGFR antibody (Tyr1068 ...
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bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Bis(sulfosuccinimidyl)suberate disodium salt (BS3; Dojindo, #B574) was used to crosslink the antibodies to protein G magnetic beads (Cell Signaling Technology). Benzonase (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... HER2 protein detection on EVs was performed by staining samples with HER2/ErbB2 (29D8) – PE-conjugated antibody or Isotype Control (Cell Signaling Technology) and CellMask plasma membrane stains (C10046 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein lysates were separated by SDS-PAGE and proteins of interests detected with the following antibodies: phosphorylated (p-VASP) Ser239 (rabbit, 1:1000, Cell Signaling #3114S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Biophysics 2023Quote: ... blocked with 5% bovine serum albumin for 1 hour at room temperature and incubated overnight with a primary antibody against the principal ventricular GJ protein Cx43 phosphorylated at Ser368 (pCx43, 1:1000, #3511S, Cell Signaling Technologies), at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h at room temperature and blotted with an antibody against phosphorylated upstream frameshift protein 1 (phospho-UPF1) (pUPF1; 1:1000, Cell Signaling Technologies) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... cFos protein was labelled in the same manner using rabbit polyclonal antibody c-Fos (9F6) (1: 1000, Cell Signaling Technology Inc, 2250) and Alexa Fluor 488 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... and then the supernatant fraction was incubated with anti-BCR-ABL antibody overnight at 4°C and subsequently with protein A magnetic beads (73778, Cell Signaling Technology) for another 4 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... containing protease inhibitor cocktail (Cell Signaling Technology) and incubated on ice for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... containing protease inhibitor PMSF (Cell Signaling Technology). Total protein was extracted in sample buffer (Cell Signaling Technology) ...
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bioRxiv - Cell Biology 2022Quote: ... containing protease inhibitor cocktail (Cell Signaling Technology) and quantified using bicinchoninic acid assay (BCA assay ...
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bioRxiv - Neuroscience 2022Quote: ... containing protease/phosphatase inhibitors (Cell Signaling #5872). Triton X-100 was added to a final concentration of 1% ...
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bioRxiv - Physiology 2019Quote: ... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... membranes were then incubated at 4°C overnight in Odyssey Blocking buffer containing 0.1% Tween 20 and the following antibodies: ERK 1/2 (mouse monoclonal, Cell Signaling Technology no. 4696; 1:1000 dilution), pERK 1/2 (rabbit monoclonal ...
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bioRxiv - Microbiology 2021Quote: ... Antibody labelling was performed with mouse anti-SARS-CoV-2 S protein (Abcalis, clone AB68-A09, #ABK68-A09-M) and secondary antibody anti-mouse Alexa488 (Cell Signaling Technology, #4408), each step followed by three washing steps with PBS containing 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins of interest were detected with HRP-conjugated horse anti-mouse IgG antibody (1:5000, 7076S, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA) or HRP-conjugated goat anti-rabbit IgG antibody (1:5000 ...
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bioRxiv - Biophysics 2021Quote: The phosphorylation of proteins was quantified by western blotting using antibodies against pEGFR (#4407 for pY1173 and #3777 for pY1068; Cell Signaling Technology (CST)) ...
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bioRxiv - Neuroscience 2019Quote: ... Formalin fixed brains were paraffin embedded and analyzed using the following antibodies according to immunohistochemistry protocols described previously (Chakrabarty, 2010; Chakrabarty, 2015): Glial Fibrillary Acidic Protein (GFAP, Cell Signaling, 1:1000) and ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cancer Biology 2019Quote: ... 15 μg of extracted proteins were separated by SDS-PAGE and revealed by antibodies directed against actin (1: 1000, Cell Signaling Technology #8457), GAPDH (1 ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein of interest was detected using an appropriate antibody specific for phospho-EGFR (Tyr1068) (2234; Cell Signaling Technology [CST], Danvers, MA, USA), EGFR (2232 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit monoclonal anti-SorLA antibody specific to the N-terminus of human SorLA protein (1:1,000; D8D4G #79322; Cell Signaling, Danvers, MA, USA) for the detection of SorLA759 proteins ...
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bioRxiv - Biochemistry 2022Quote: ... phosphorylated at Ser293 PDHA and acetylated proteins were estimated by western-blotting using primary antibodies from Cell Signaling Technology (Danvers, MA, United States) with the product numbers #5490 ...
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bioRxiv - Immunology 2022Quote: ... Cas9 expression was validated by flow cytometry using intracellular staining of cas9 protein with anti-cas9 monoclonal antibody (7A9-3A3, Cell Signaling, cat #48796). Cas9 activity was assessed by transducing the cas9-BV2 cells with pXPR_047 ...
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bioRxiv - Genomics 2023Quote: ... Correct expression of the mAID-Dam-Lamin B1 fusion protein as well as the wildtype Lamin B1 protein was confirmed using Immunofluorescence staining using an anti-HA antibody at 1 in 500 dilution (Cell Signaling Technologies, C29F4) and an anti-LaminB1 antibody at 1 in 500 dilution (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Biophysics 2023Quote: ... mAb #3288) and Lysosome Associated Membrane Protein 1 (LAMP1) specific rabbit monoclonal antibody (cat. no. mAb #9091) were purchased from Cell Signaling Technology (USA). Anti-caveolin-1 rabbit polyclonal antibody was acquired from BD Biosciences (USA ...
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bioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA protein assay kit from Cell Signaling Technology. Thirty micrograms of total protein were mixed in a reducing sample buffer and used for mitochondrial apoptosis assay per the kit instruction ...
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bioRxiv - Pathology 2021Quote: ... the membranes were incubated in a blocking buffer containing the appropriate diluent of primary antibodies against apoA5 (#3335, Cell Signaling Technology, USA; sc-373950, Santa Cruz, USA), tubulin (66031-1-lg ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated for 48 hr at 4C in 1× PBS containing the primary antibodies (Chicken anti-GFP, AbCam cat. No. #13970. 1:2000; Rabbit anti-NeuN, Cell Signaling Technology, cat. No. #24307, 1:1000). Slices were then washed 3 × 5 minutes in 1x PBS and incubated in 1x PBS containing the secondary antibodies for 2 hours (Alexa Fluor 488 ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated in primary antibody solutions consisting of fresh blocking solution containing CCNT1 antisera (Santa Cruz Biosciences, Dallas, TX, USA, sc-398695; Cell Signaling Technologies, Danvers, MA, USA D1B6G), CDK9 antisera (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 1 M dithiothreitol (DTT) (#7016, Cell Signaling) and protease inhibitor cocktail (PIC ...
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bioRxiv - Microbiology 2019Quote: ... containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysate was incubated on ice one hour ...
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bioRxiv - Genetics 2020Quote: ... containing 15% Fetal Bovine Serum (Cell Signaling Technologies), 1000U/mL Leukemia Inhibitory Factor (Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... Experimental cultures received media containing DOX (Cell Signaling) at 1μM whilst control cultures received media containing DMSO ...
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bioRxiv - Cancer Biology 2023Quote: ... containing phosphatase inhibitors (Cell Signaling Technology, Cat#5870). Samples were kept on ice for 30 minbefore being sonicated using a water bath sonicator for 1 min at low intensity ...
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bioRxiv - Neuroscience 2023Quote: ... containing protease/phosphatase inhibitors (Cell Signaling, Cat#5872). Triton X-100 (Sigma ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
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bioRxiv - Microbiology 2020Quote: ... of the sample was incubated with 3 μg of antibody (or 3 μg of IgG as control) and 30 μl of protein G magnetic beads (Cell Signaling Technology, Cat. 7008) at 4 °C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... In order to positively identify the proteins, the following antibodies were used: MAN1B1 (SC-100543, 1:500) and actin (1:2000) (Cell Signaling Technology, Beverly, MA). Horse radish peroxidase (HRP)-conjugated secondary antibodies were incubated with the membrane at RT for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000, Cell Signaling Technology, #8954), anti V5-HRP (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... we validated the presence of the correctly sized tagged MYC protein and absence of wild type MYC by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). We selected homozygous clone number 2 (for N-BioTAP-cMYC ...
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bioRxiv - Immunology 2022Quote: ... The expression levels of target proteins were assessed by western blotting (Figure S1) with antibodies against ADA (clone D1P4Y, Cell Signaling Technology, Cat#65184), ADSSL1 (clone 2D12 ...
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bioRxiv - Plant Biology 2023Quote: ... The bead-bound proteins were separated by SDS-PAGE and detected by western blotting with the MBP antibody (Cell Signaling Technology, Danvers, MA, USA), His-tag antibody (Tiangen ...
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bioRxiv - Microbiology 2023Quote: ... Target proteins were detected by various primary antibodies (For phosphor-CREB rabbit mAb, Cell Signaling Technology #9198; For CREB rabbit mAb, Cell Signaling Technology #4820 ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...