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Citations for Cell Signaling Technology :
551 - 600 of 952 citations for Total Phenols Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: 500 μg of unstimulated HT29 lysate was immunoprecipitated onto Protein A/G coated wells (Pierce #15138) following the kinase assay protocol but with 0.5 μg of IKKα (Cell Signaling Technology #2682) or 0.5 μg of naïve rabbit IgG (Jackson Immunoresearch #011-000-002) ...
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bioRxiv - Immunology 2021Quote: ... Western blotting was used as the output assay to determine levels of the ribosomal protein S6 kinase (p-S6K) using an S6K and p-S6K antibodies (Cell Signaling Technology). To assess the impact of mTOR1 inhibition rapamycin (20ng/mL ...
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of puromycin for 48 hours and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of neomycin for 48 hours and then used for HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure MyD88 and TRIF expression using a rabbit monoclonal antibody to MyD88 (Cell Signaling Technology), a rabbit polyclonal antibody to TRIF (Cell Signaling Technology) ...
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bioRxiv - Immunology 2022Quote: ChIP assays for histone modifications were performed as described previously (Aoyama et al., 2018) using an anti-H2AK119ub1 (#8240S; Cell Signaling Technology) and an anti-H3K27me3 (#07-449 ...
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bioRxiv - Cancer Biology 2022Quote: ... were used in Western blot assays in xenograft pharmacodynamic (PD) studies: SYK (AB_2687924) (rabbit mAb, Cell Signaling Technology, Cat# 13198, 1:1000), EGFR (AB_2230881 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were harvested at indicated time points and lysed in 1× radioimmunoprecipitation assay (RIPA) buffer (Cell Signaling, Danvers, MA, USA; Cat# 501015489) containing 1× protease inhibitor cocktail (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... Radioimmunoprecipitation assay buffer (RIPA) 10x and the ROCK inhibitor Y-27632 (cat# 13624S) were purchased from Cell Signaling Technologies (Whitby, ON, Canada). BrainPhys™ Neuronal Medium was obtained from STEMCELL Technologies (Vancouver ...
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bioRxiv - Genomics 2020Quote: ... and subjected to immunoblotting with the antibodies for phosphorylated Erk-1/2 (Thr202/Tyr 204) or total Erk-1/2 from Cell Signaling Technology (Beverly, MA). Blots were visualized by enhanced chemiluminescence western detection system (Pierce ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole protein lysates were analyzed for total protein expression and lysine-methylated peptides were enriched using reagents from Cell Signaling Technology (catalogue no. 14809). In short ...
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bioRxiv - Microbiology 2021Quote: ... The extent of protein knockdown was determined by immunoblotting and total loading was detected by probing the blots with an anti-GAPDH antibody (Cell Signaling; #5174, clone D16H11).
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bioRxiv - Molecular Biology 2019Quote: ... Tween and detection of total CHK1 or CHK2 proteins was carried out for 1 h at RT using mouse-anti-Chk1 mAb (2G1D5) (Cell Signaling 2360; Lot 3) or mouse anti-Chk2 mouse mAb (BD Biosciences 611570) ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... Membranes were then incubated with primary antibody diluted in TBS-T buffer (1:1000 Total OXPHOS Rodent WB Antibody Cocktail [abcam Cat# ab110413] or 1:1000 HSP60 XP Rabbit mAB [Cell Signaling Technology CAT#12165]) overnight at 4°C with gentle rocking ...
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bioRxiv - Molecular Biology 2021Quote: Cell proliferation was examined using DNA synthesis analysis (bromodeoxyuridine (BrdU) incorporation assay) or Ki67 detection with specific antibody (Cell Signaling Technology, Danvers, MA) as described previously (7 ...
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bioRxiv - Cancer Biology 2022Quote: Whole cell protein was extracted from cells treated for 24 h with 1 mM aspirin and 4 μM regorafenib using radio-immunoprecipitation assay buffer (Cell Signaling Cat# 9806) supplemented with 1 mM phenylmethanesulfonyl fluoride (Cell Signaling Cat# 8553) ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used for cleavage under targets & release using nuclease (CUT&RUN) assays: anti-H3K27ac (8173, Cell Signaling Technology, 1:100) and anti-H3K4me1 (5326 ...
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bioRxiv - Molecular Biology 2020Quote: Cell were washed twice with phosphate-buffered saline and lysed with sonication in radioimmunoprecipitation assay (RIPA) buffer (#9806, Cell Signaling, Danvers, MA, USA), before being incubated on ice for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... boiled in sample buffer for 5 min and subjected to immunoblot assay with anti-HA antibody (Cell Signaling Technology, Inc., #3724, Danvers, MA).
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bioRxiv - Neuroscience 2023Quote: ... cultured primary pericytes were lysed with cold lysis buffer of radioimmunoprecipitation assay (RIPA) lysing buffer supplemented with 1x protease/phosphatase inhibitor (Cell Signaling Technology; U.S.A). Cells were mechanically agitated for 5 minutes and incubated on ice for a further 30 minutes with occasional agitation ...
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bioRxiv - Molecular Biology 2024Quote: All cargo constructs include an epitope tag or tandem tag (FLAG or 3xFLAG) for assay by anti-FLAG primary antibodies (Cell Signaling Technology, 2368). HEK293FT cells were transfected at 24 well ...
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bioRxiv - Neuroscience 2024Quote: ... 96-well Meso Scale Discovery assay plates (MSD, L15XA-6) were coated with 30 μl of pS65-Ub capturing antibody (Cell Signaling Technology, 62802) with a final concentration of 1 µg/ml in 200 mM sodium carbonate coating buffer (pH 9.7 ...
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bioRxiv - Developmental Biology 2021Quote: ... A SignalStain DAB Substrate Kit (Cell Signaling Technology) was used for chromogenic antibody detection ...
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bioRxiv - Cancer Biology 2022Quote: ... Senescence β-Galactosidase Staining Kit (Cell Signaling Technology), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and detected using ECL detection kit (Cell Signaling). A summary of antibodies used is presented in Supplementary Information Table S3.
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bioRxiv - Physiology 2023Quote: ... and cAMP ELISA kit (Cell Signaling Technology, #4339) were used to measure these cyclic nucleotide second messengers from adult A ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies against PRDM1 (1:750 dilution, gift from Dr. Philip W. Ingham)46 and total histone H3 (1:5000 dilution, Cell Signaling Technology, Danvers, MA, #9715) were used ...
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bioRxiv - Systems Biology 2022Quote: ... Protein was solubilized in RIPA buffer and then subject to SDS-PAGE and immunoblotting with antibodies to total SRC and phospo-Y416 SRC (Cell Signaling, 2110 and 6943 respectively). Membranes were scanned using a Licor Odyssey fluorescent imaging instrument and densitometry conducted using Image Studio Lite ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was washed again 4 times in TBST for 5 m each and blocked again before incubating with primary antibodies: total Akt (1:1000, Cell Signaling Technology, Cat. #9272S, RRID:AB_329827) & total S6RP (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies used included the following: rabbit anti-TCF7L2 (1:1,000 for western blotting and 2 μg for co-IP assays; #2569; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-TCF7 (1:1,000 for western blotting ...
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bioRxiv - Cell Biology 2020Quote: SMC were seeded at a density of 75000/well in 6 well plates and cultured in FGM for 48 h prior to fixation and staining using a commercial β-galactosidase assay (Cell Signaling Technologies #9860) as described previously (41) ...
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bioRxiv - Cancer Biology 2022Quote: The cells were harvested and lysed using radioimmunoprecipitation assay (RIPA) buffer supplemented with PMSF (1mM) and protease inhibitor cocktail (Cell Signaling Technology, Massachusetts, USA). The protein concentration was determined using Bradford protein assay (Sigma ...
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bioRxiv - Immunology 2023Quote: 18 µL U-87 MG EVs that had been separated by UC or SEC were lysed in 1x radioimmunoprecipitation assay buffer (RIPA, Cell Signaling Technology, catalog #9806) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... anti-Actin and HRP- labeled goat-anti-rabbit or anti-mouse IgGs for Western blot assay were purchased from Cell Signaling Technology (Danvers, MA). Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Neuroscience 2021Quote: Senescence β-Galactosidase Staining Kit (9860, Cell Signaling technology) was applied to measure the senescent monolayer cultured neural progenitor cells according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... rabbit anti-Phospho-KIT (Try719) antibody (#3391; Cell Signaling), rabbit anti-Green Fluorescent Protein antibody (AB3080P ...
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bioRxiv - Neuroscience 2020Quote: ... The PathScan®Immune antibody array kit (Cell Signaling) was used according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ChIP was performed using ChIP kit (Cell signaling, 57976s). Briefly ...
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bioRxiv - Immunology 2020Quote: ... ChIP was conducted using SimpleChIP kit (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... ER Stress Antibody Sampler Kit (#9956, Cell Signaling Technology), Sec24C (#14676 ...
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bioRxiv - Developmental Biology 2022Quote: ... SimpleChIP plus enzymatic chromatin IP kit (Cell Signaling Technologies) was used for ChIP experiment as per manufacturer recommendations and PCR was used to detect ChIP products run out on agarose gel.
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bioRxiv - Cancer Biology 2023Quote: ... and digested using the PTMScan Kit Protocol (Cell Signaling). Peptide purification was performed using Sep-Pak® C18 columns (Waters) ...
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bioRxiv - Physiology 2023Quote: A competitive cGMP ELISA kit (Cell Signaling Technology, #4360) and cAMP ELISA kit (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... The SA-b-gal staining kit (#9860, Cell Signaling) was performed according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Western blots for detecting MAP kinase phosphorylation were probed with primary antibodies against phospho-MAPK1/3 (Thr202/Tyr204) and total MAPK1/3 (Cell Signaling Technology, #4370 and #4696, respectively). MAPK1 is also known as ERK2 or p42MAPK ...
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bioRxiv - Neuroscience 2022Quote: ... NF-KB (1:1000 mouse anti-total NF-κB; Cell Signaling Technology #6956; RRID #AB_10828935 or 1:1000 rabbit anti-phospho NF-κB-S536; Cell Signaling Technology #3033; RRID #AB_331284); Akt (1:250 mouse anti-total Akt ...
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bioRxiv - Immunology 2024Quote: ... Anti-AhR and anti-5-loxygenase (5-LOX) antibodies (Ab) for immunoblot assay and anti-trimethyl H3K4 (H3K4me3) Ab for chromatin immunoprecipitation (ChIP) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-ALOX5AP (FLAP ...
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bioRxiv - Developmental Biology 2021Quote: ... HTPCs at P6 and P 15 were seeded onto glass cover slips and stained with a commercial kit (Senescence β-Galactosidase Staining Kit, Cell Signaling Technology) according to the manufacturer’s instructions and as described before(Schmid et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Magnetic beads for all experiments come from PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology, Kit #59322), along with 1X HS IAP Bind Buffer #1 and 1X HS IAP Wash Buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The histone H3Ac antibody sampler kit (9927T) and acetylated Histone H4 antibody sampler kit (ab218056) used were purchased from Cell Signaling Technology (CST) and abcam (USA) ...