Labshake search
Citations for Cell Signaling Technology :
251 - 300 of 7993 citations for Surfactant Protein D SFTPD Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The spike protein and fragments were detected by rabbit monoclonal antibodies (mAb) against the 6-His tag (12698S) from Cell Signaling Technology. The nitrocellulose (NC ...
-
bioRxiv - Microbiology 2023Quote: ... The phosphorylated proteins were detected by probing the membranes with an anti-phospho-c-Met antibody (Tyr1234/1235, #3077, Cell Signaling Technology) or an anti-phospho-EGFR antibody (Tyr1068 ...
-
bioRxiv - Biophysics 2023Quote: ... blocked with 5% bovine serum albumin for 1 hour at room temperature and incubated overnight with a primary antibody against the principal ventricular GJ protein Cx43 phosphorylated at Ser368 (pCx43, 1:1000, #3511S, Cell Signaling Technologies), at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
-
bioRxiv - Cell Biology 2023Quote: ... Bis(sulfosuccinimidyl)suberate disodium salt (BS3; Dojindo, #B574) was used to crosslink the antibodies to protein G magnetic beads (Cell Signaling Technology). Benzonase (Merck ...
-
bioRxiv - Cancer Biology 2023Quote: ... HER2 protein detection on EVs was performed by staining samples with HER2/ErbB2 (29D8) – PE-conjugated antibody or Isotype Control (Cell Signaling Technology) and CellMask plasma membrane stains (C10046 ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein lysates were separated by SDS-PAGE and proteins of interests detected with the following antibodies: phosphorylated (p-VASP) Ser239 (rabbit, 1:1000, Cell Signaling #3114S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
-
bioRxiv - Molecular Biology 2024Quote: ... for 1 h at room temperature and blotted with an antibody against phosphorylated upstream frameshift protein 1 (phospho-UPF1) (pUPF1; 1:1000, Cell Signaling Technologies) overnight at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... cFos protein was labelled in the same manner using rabbit polyclonal antibody c-Fos (9F6) (1: 1000, Cell Signaling Technology Inc, 2250) and Alexa Fluor 488 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit (1:200 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and then the supernatant fraction was incubated with anti-BCR-ABL antibody overnight at 4°C and subsequently with protein A magnetic beads (73778, Cell Signaling Technology) for another 4 h at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Gasdermin D (1:1000, 93709, RRID: AB_2800210, Cell Signaling Technology), MLKL (1:1000 ...
-
bioRxiv - Microbiology 2021Quote: ... anti-Gasdermin-D (anti-rabbit, 1:1000; #93709, Cell Signaling), anti-Viperin (anti-rabbit ...
-
bioRxiv - Physiology 2019Quote: ... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Antibody labelling was performed with mouse anti-SARS-CoV-2 S protein (Abcalis, clone AB68-A09, #ABK68-A09-M) and secondary antibody anti-mouse Alexa488 (Cell Signaling Technology, #4408), each step followed by three washing steps with PBS containing 0.05% Tween-20 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins of interest were detected with HRP-conjugated horse anti-mouse IgG antibody (1:5000, 7076S, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA) or HRP-conjugated goat anti-rabbit IgG antibody (1:5000 ...
-
bioRxiv - Biophysics 2021Quote: The phosphorylation of proteins was quantified by western blotting using antibodies against pEGFR (#4407 for pY1173 and #3777 for pY1068; Cell Signaling Technology (CST)) ...
-
bioRxiv - Neuroscience 2019Quote: ... Formalin fixed brains were paraffin embedded and analyzed using the following antibodies according to immunohistochemistry protocols described previously (Chakrabarty, 2010; Chakrabarty, 2015): Glial Fibrillary Acidic Protein (GFAP, Cell Signaling, 1:1000) and ionized calcium binding adaptor molecule 1 (Iba1 ...
-
bioRxiv - Cancer Biology 2019Quote: ... 15 μg of extracted proteins were separated by SDS-PAGE and revealed by antibodies directed against actin (1: 1000, Cell Signaling Technology #8457), GAPDH (1 ...
-
bioRxiv - Cancer Biology 2020Quote: ... The protein of interest was detected using an appropriate antibody specific for phospho-EGFR (Tyr1068) (2234; Cell Signaling Technology [CST], Danvers, MA, USA), EGFR (2232 ...
-
bioRxiv - Cell Biology 2021Quote: ... rabbit monoclonal anti-SorLA antibody specific to the N-terminus of human SorLA protein (1:1,000; D8D4G #79322; Cell Signaling, Danvers, MA, USA) for the detection of SorLA759 proteins ...
-
bioRxiv - Biochemistry 2022Quote: ... phosphorylated at Ser293 PDHA and acetylated proteins were estimated by western-blotting using primary antibodies from Cell Signaling Technology (Danvers, MA, United States) with the product numbers #5490 ...
-
bioRxiv - Immunology 2022Quote: ... Cas9 expression was validated by flow cytometry using intracellular staining of cas9 protein with anti-cas9 monoclonal antibody (7A9-3A3, Cell Signaling, cat #48796). Cas9 activity was assessed by transducing the cas9-BV2 cells with pXPR_047 ...
-
bioRxiv - Genomics 2023Quote: ... Correct expression of the mAID-Dam-Lamin B1 fusion protein as well as the wildtype Lamin B1 protein was confirmed using Immunofluorescence staining using an anti-HA antibody at 1 in 500 dilution (Cell Signaling Technologies, C29F4) and an anti-LaminB1 antibody at 1 in 500 dilution (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
-
bioRxiv - Biophysics 2023Quote: ... mAb #3288) and Lysosome Associated Membrane Protein 1 (LAMP1) specific rabbit monoclonal antibody (cat. no. mAb #9091) were purchased from Cell Signaling Technology (USA). Anti-caveolin-1 rabbit polyclonal antibody was acquired from BD Biosciences (USA ...
-
bioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA protein assay kit from Cell Signaling Technology. Thirty micrograms of total protein were mixed in a reducing sample buffer and used for mitochondrial apoptosis assay per the kit instruction ...
-
bioRxiv - Neuroscience 2021Quote: ... Rabbit anti-pMet/HGFR (1:200, Cell Signaling/R&D systems), Rabbit anti-pBTK (1:200 ...
-
bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
-
bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
-
bioRxiv - Microbiology 2020Quote: ... of the sample was incubated with 3 μg of antibody (or 3 μg of IgG as control) and 30 μl of protein G magnetic beads (Cell Signaling Technology, Cat. 7008) at 4 °C overnight ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
-
bioRxiv - Cancer Biology 2019Quote: ... In order to positively identify the proteins, the following antibodies were used: MAN1B1 (SC-100543, 1:500) and actin (1:2000) (Cell Signaling Technology, Beverly, MA). Horse radish peroxidase (HRP)-conjugated secondary antibodies were incubated with the membrane at RT for 1 hr ...
-
bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000, Cell Signaling Technology, #8954), anti V5-HRP (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... we validated the presence of the correctly sized tagged MYC protein and absence of wild type MYC by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). We selected homozygous clone number 2 (for N-BioTAP-cMYC ...
-
bioRxiv - Immunology 2022Quote: ... The expression levels of target proteins were assessed by western blotting (Figure S1) with antibodies against ADA (clone D1P4Y, Cell Signaling Technology, Cat#65184), ADSSL1 (clone 2D12 ...
-
bioRxiv - Microbiology 2023Quote: ... Target proteins were detected by various primary antibodies (For phosphor-CREB rabbit mAb, Cell Signaling Technology #9198; For CREB rabbit mAb, Cell Signaling Technology #4820 ...
-
bioRxiv - Plant Biology 2023Quote: ... The bead-bound proteins were separated by SDS-PAGE and detected by western blotting with the MBP antibody (Cell Signaling Technology, Danvers, MA, USA), His-tag antibody (Tiangen ...
-
bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
-
bioRxiv - Genomics 2023Quote: ... The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara ...
-
bioRxiv - Cancer Biology 2021Quote: ... protein lysis buffer (Cell Signaling) containing a protease inhibitor cocktail was added and the mixture homogenized ...
-
bioRxiv - Molecular Biology 2021Quote: ... The same membrane that was used to detect AIRE protein was washes and reused in an incubation with an anti-GAPDH rabbit polyclonal primary antibody (Cell Signaling Technology, Beverly, MA, USA) following incubation with peroxidase conjugated anti-rabbit antibody.
-
bioRxiv - Cancer Biology 2020Quote: ... Protein amounts of OP18 and beta-actin were quantified using a primary stathmin polyclonal antibody (1:1000; Cell Signaling Technology, NEB GmbH, Frankfurt/Main, Germany) and a polyclonal beta-actin antibody (1:1000 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibody in 10 % Donkey Serum in PBS overnight at 4 °C: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb (Cell Signaling Technology, Cat# 5364; RRID:AB_10694233). After HSCs were washed three times with PBS + 0.1 % Tween-20 ...
-
bioRxiv - Cell Biology 2021Quote: ... Forty-five μg of protein were resolved in SDS-PAGE and blotted with anti-phospho-eNOS (Ser1177) antibodies (Cell Signaling, Danvers, MA, USA, 1:250), followed by incubation with goat anti-rabbit horseradish peroxidase secondary antibodies (Jackson ImmunoResearch ...
-
bioRxiv - Developmental Biology 2021Quote: ... 20 μg of protein per lane were subjected to SDS-PAGE and probed with the following primary antibodies: YAP (Cell Signaling Technologies #8418, 1:500), YAP/TAZ (Cell Signaling #4912 ...
-
bioRxiv - Physiology 2023Quote: ... we loaded 25 µg protein of each sample to SDS-PAGE gels and incubated the membranes with 1:1000 eIF2α antibody (Cell Signaling Technology #9722, Danvers, MA) or 1:1000 Phospho-eIF2α antibody (Cell Signaling Technology #9721 ...
-
bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were extracted at 3 hours post-treatment and analysed by western blot following overnight incubation with antibodies against the following primary proteins: Vinculin (E1E9V, rabbit, Cell Signaling Technology, 13901, 1:1000), Phosphorylated p44/42 MAPK (ERK1/2 ...
-
Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... rabbit anti-Cathepsin D (E179) (Cell Signaling Technology Cat# 69854, 1:1000), goat anti-Biotin (Thermo Fisher Scientific Cat# 31852 ...
-
bioRxiv - Biochemistry 2022Quote: ... Primary antibodies (rabbit insulin antibody: #4590 Cell Signaling Technology, OC (generic amyloid antibody) and rabbit β-actin antibody ...