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Citations for Cell Signaling Technology :
151 - 186 of 186 citations for Steroid Receptor Co Factor Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Kε-GG peptides were enriched using the PTMScan HS Ubiquitin/SUMO Remnant Motif (Kε-GG) Kit from Cell Signaling Technologies using the provided reagents and protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed and permeabilized with the Foxp3 transcription factor staining kit (eBioscience, 00-5523-00) prior intracellular staining with the indicated antibodies: H3K9ac (Cell Signaling, 9649, 1:3000), H3K27ac (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... penicillin/streptomycin and 2% Fetal bovine serum (for vehicle group) or the same media with 100ng/ml recombinant colony-stimulating factor 1 (Csf1, Cell Signaling, Boston, MA, USA). Csf1 stock was diluted in saline.
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bioRxiv - Cancer Biology 2024Quote: ... The following antibodies were used for Western blots with the indicated dilution factors: Hexokinase I (C35C4) Rabbit mAb (Cell Signaling Technology, Cat# 2024, RRID:AB_2116996) at 1:1,000 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were washed twice with PBS-BSA and then co-stained for 20 minutes with anti-myc Alexa 647 (clone 9B11, 1/250 dilution; Cell Signaling Technology) and anti-HIS-FITC (chicken polyclonal ...
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bioRxiv - Physiology 2022Quote: ... The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling, 4412S) at 1:1000 dilution at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were resuspended in 1ml total volume cold Co-IP Buffer containing rabbit IgG or rabbit anti-FLAG antibody (Cell Signaling #14793S), 4μg per sample ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked with Odyssey blocking buffer (LICOR, Omaha, NE, USA) and incubated overnight with an antibody against hypoxia inducible factor-1α (HIF-1α, Cell Signaling Technology #36169; 1:500). Proteins were visualized using IRDye 800CW secondary antibodies (LICOR ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated for 2 h in a solution of TBST (0.05%) and LI-COR blocking buffer (5%) containing the following antibodies: anti-ERK1/2 (Cell Signaling Technologies; dilution factor: 1/2000) and streptavidin IRdye680 (LI-COR Biosciences ...
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bioRxiv - Systems Biology 2019Quote: ... and monomethyl arginine peptides were immunoprecipitated by addition of 40 uL of PTMScan Symmetric Di-Methyl Arginine Motif Kit (13563, Cell Signaling), PTMScan Asymmetric Di-Methyl Arginine Motif Kit (13474 ...
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bioRxiv - Systems Biology 2020Quote: ... DiGly remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-ɛ-GG) Kit (Cell Signaling Technology (CST)) ...
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bioRxiv - Plant Biology 2019Quote: ... and the interaction between PNPAR and the PNP-A synthetic peptide was determined by immunoblot analysis using a mouse anti-MYC monoclonal antibody (1:10,000; Cell Signaling Technology) and a mouse anti-biotin monoclonal antibody (1:2,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Ubiquitinated peptides were enriched by immunoaffinity purification using the PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology, 5562) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit monoclonal antibody to a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is acetylated, Cell Signaling) were used.
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bioRxiv - Cell Biology 2024Quote: ... co-housed 6-month-old female C57BL/6J wildtype mice were administered either: 1) 6mg/kg of Necrostatin1s (Cell Signaling Technologies Cat#17802) in dimethyl sulfoxide via intraperitoneal injection then 15-30 minutes later given 150 μg/kg TNF (BioTimes Inc ...
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bioRxiv - Cell Biology 2020Quote: K-ε-GG remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-Є-GG) Kit (Cell Signaling Technology). Crosslinking of antibodies to beads and subsequent immunopurification was performed with slight modifications as previously described [62] ...
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bioRxiv - Molecular Biology 2021Quote: ... quantified with Quantitative Colorimetric Peptide Assay (Thermo Fischer Scientific) and dried on Speed-Vacuum before enrichment with Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling). After Sep Pak desalting ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for acetylation with anti-acetyl antibody conjugated to agarose beads (Acetyl-Lysine Motif Kit; Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Biochemistry 2022Quote: Concentrated stock of isotopically labelled internal standard (heavy) peptides (M1, M1ox, K6, K6ox, K11, K27, K29, K33, K48, K63) were purchased from Cell Signaling Technologies and Cambridge Research Biochemicals (Cleveland ...
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bioRxiv - Molecular Biology 2020Quote: ... the rabbit monoclonal anti-Actin antibody (12748S, rabbit monoclonal antibody to a synthetic peptide corresponding to residues near the carboxy terminus of human β-actin protein, Cell Signaling) and the rabbit monoclonal anti-acetyl-histone H3 (Lys9 ...
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bioRxiv - Cell Biology 2021Quote: Acylated (either acetylated or malonylated) peptides were enriched by immunoaffinity purification using the PTMScan Acetyl-Lysine or PTMScan Malonyl-Lysine Kits (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining 90 μg of each sample was pooled for phospho-peptide enrichment with PTMScan Phospho-Enrichment IMAC FE-NTA Magnetic Beads (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Dry peptides were then resuspended in an IAP buffer (Cell Signalling #59322) and incubated with anti-diGly antibody-conjugated slurry (Cell Signaling #59322) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Enrichment for ubiquitinated peptides was conducted using a PTM Scan® Ubiquitin Remnant Motif [K-ε-GG] kit (Cell Signaling, Danvers, MA) following the vendor’s recommended procedures ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies used included the following: rabbit anti-TCF7L2 (1:1,000 for western blotting and 2 μg for co-IP assays; #2569; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-TCF7 (1:1,000 for western blotting ...
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bioRxiv - Neuroscience 2022Quote: The phospho-dependent association of overexpressed nuclear Jacob was assessed by heterologous co-immunoprecipitation from HEK293T cells pre-treated with 20 nM staurosporine (Cell Signaling Technology, Cat.: #9953S) for 3 h.
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bioRxiv - Neuroscience 2023Quote: 25µm coronal sections through the striatum were prepared using a microtome and were co-stained with rabbit anti-pSer129-Syn (Cell Signaling #23706; 1:500) and chicken anti-TH (Millipore #AB9702 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were alkylated and digested with trypsin at 37°C overnight and ubiquitinated peptides were enriched with PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology). After Sep Pak desalting ...
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bioRxiv - Microbiology 2021Quote: ... and sera from individual animals were tested by ELISA with KLH-conjugated peptide immobilized on plastic surface and anti-mouse or anti-rabbit horse radish peroxidase (HRP)-conjugated detector Ab (Cell Signaling Technology, USA). The animals with the highest Ab titer were selected for booster immunization ...
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bioRxiv - Systems Biology 2024Quote: The enrichment of lysine acetylated peptides was performed using a PTMScan HS Acetyl-Lysine Motif [Ac-K] Kit (Cell Signaling Technology, Cat #50071) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The rest of the eluate was dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology) as described in the manual ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole protein lysates were analyzed for total protein expression and lysine-methylated peptides were enriched using reagents from Cell Signaling Technology (catalogue no. 14809). In short ...
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bioRxiv - Molecular Biology 2021Quote: Enrichment of K-ε-GG peptides was performed with the PTMScan ubiquitin remnant motif (K-ε-GG) kit (Cell Signaling Technology, cat. no. 5562) containing anti–K-ε-GG antibody cross-linked to protein A beads ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for malonylation with anti-malonyl antibody conjugated to agarose beads from the Malonyl-Lysine Motif Kit (Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... peptide samples resuspended in IAP buffer were incubated with 100uL of antibody-bead conjugates specific for acetyl-lysine (Cell Signaling Technologies, PTMScan kits #13416). Immunoprecipitation proceeded overnight at 4°C with gentle mixing ...
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bioRxiv - Microbiology 2020Quote: ... Immunodetection was carried out by using rabbit anti-γ-H2A.X (SPQ) primary antibody (generated using peptide containing the SPQ motif, Cell signaling cat # 9718S, 1:1000), anti-H2A antibody (Abcam cat# ab88770 ...