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Citations for Cell Signaling Technology :
151 - 200 of 7932 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with either 5% skimmed milk or 5% bovine-serum albumin (BSA) and incubated with dilution of respective antibodies as per manufacturer’s instructions (Cell Signaling technologies). The membrane was washed ...
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bioRxiv - Cell Biology 2021Quote: ... The protein A agarose bead was added with 450 µl of IP buffer and 5 µl of Myc antibody (Cell Signaling). The mixture was then incubated on rotator at 4°C for 4 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were then incubated o/n at 4C with 5% milk powder in PBS-T containing the primary antibodies at the following dilutions (1:1000 Lin28a (A177, Cell signaling); 1:4000 H3 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T, #9102, Cell Signaling) and phospho-p44/42 MAPK (Thr202/Tyr204 ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies (in 5% milk in TBST) were used for western blotting: anti-E-cadherin (#14472, monoclonal mouse, 1:1000, Cell Signaling), anti-N-cadherin (#BDB610920 ...
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bioRxiv - Immunology 2021Quote: ... Cells were treated with 5% goat serum for 30 min and incubated with antibodies against TRPC3 (Alomone Labs) or NFkB-p65 (Cell Signaling) for 1h ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was blocked for 1h with 5% BSA in TBS-T followed by overnight incubation at 4°C with antibody (Drosha 1:1000; D28B1, Cell Signaling, in 5 %BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... The chromatin was then incubated with 5 µg of antibody against KDM6A (A302-374A, Bethyl Laboratories and 33510S, Cell Signaling Technology), KDM6B (ab169197 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by blocking in 5% BSA with 0.2% Triton X-100 for 30min and incubation with primary antibody Axin1 (#2087, 1:200, Cell Signaling Technology) overnight ...
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bioRxiv - Plant Biology 2023Quote: ... Blots for detecting phosphorylated MAPK were blocked in 5% BSA and immunoblotted with a phosphor-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling). Horseradish peroxidase-conjugated anti-rabbit antibody produced in goat (Agrisera ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed 3x 5 mins with PBS and then stained with corresponding secondary antibodies (1:1000; 4414S Cell Signaling Technology, 8889S Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking solution was replaced with 1° antibody (Ab) (Phospho-Smad1/5/9 Rabbit mAb #13820, Cell Signaling Technology 13820T, 1:300) in 10%NGS/PBS-Tx and the slides were incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... All antibodies were diluted in 1X PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membrane was shaken three times for 5 min in TBST then incubated with 1:3,000 anti-rabbit HRP-linked antibody (Cell Signaling Technologies) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies were diluted 1:1000 in 5% milk/TBST (FGFR1: D8E4; GAPDH HRP-conjugated: 14C10; Cell Signaling, Danvers, MA, USA). The membranes were incubated with antibodies at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked for 1h and probed with 5% nonfat dry milk with the following primary antibodies: anti-IRF8 (clone D20D8, Cat# 5628, Cell Signaling), anti-CD74 (sheep anti-mouse cd74 ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were blocked with 3% BSA in PBS overnight at 4°C and then incubated with a rabbit phospho-SMAD1/5/9 antibody at 1:400 (D5B10, Cell Signaling) and a mouse anti-YAP antibody at 1:200 (63.7 ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell pellets were washed with ice cold PBS and resuspended in 1X Annexin V binding buffer (Cell Signaling Technology, USA). Cells were then incubated with Annexin V-FITC conjugate (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were normalized by bicinchoninic acid (BCA) assay (Cell Signaling, 7780), and loaded on a precast SDS-PAGE gel (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were normalized by bicinchoninic acid (BCA) assay (Cell Signaling, 7780), and loaded on a precast SDS-PAGE gel (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858), S6 (Cell Signaling 2217) ...
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bioRxiv - Cell Biology 2020Quote: For immunoblotting the following primary antibodies were used at a concentration of 1:1000 in 5 % milk-TBS-T: HA (Cell Signaling 3724S), HA (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% milk and incubated in 2% BSA with primary antibodies to GAPDH (Cell Signaling Technology Cat# 13084, RRID:AB_2713924), Eg5 (Sigma Aldrich Cat# SAB4501650 ...
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bioRxiv - Molecular Biology 2021Quote: ... washed three times with 1x PBS and incubated with 5 % horse serum/1xPBS for 1 h at 20 °C before incubating with the following antibodies (Cell Signaling Laboratories): anti-calnexin (clone C5C9) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibody incubations were done in 5% milk/PBS using rabbit anti-HA at 1:500 (Cell Signaling, Cat #: 3724S) and anti-Rabbit HRP at 1:10,000 (BioRad ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were washed four times with TBST (5 minutes gentle rocking per wash) and incubated overnight with primary antibody Rabbit anti-Gli-1 (Cell signaling, #C68H3); 1:250 dilution in TBST-Milk at 4°C with gentle rocking ...
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bioRxiv - Genomics 2020Quote: ... We also validated protein expression and inducibility of the KRABINER rescue transgenes via western blot (mouse anti-myc antibody ThermoFisher #MA1-21316, 1:1000 dilution in 5% milk/TBST; rabbit anti-β-actin loading control Cell Signaling #8457 ...
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bioRxiv - Biochemistry 2021Quote: ... Slices were blocked with 5% donkey serum (37°C, 30 min) and then incubated with a primary antibody targeting RIPK3 (#95702, Cell Signaling Technology) at 4°C overnight diluted in antibody diluent (Dako) ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked with 5% non-fat milk/PBS for 30 min and incubated with primary antibodies as follows: anti-PDGFRA (Cell Signaling, 3174T), anti-COL1A1 (Proteintech ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 3% BSA or 5% milk and probed with the following primary antibodies-1:1000 rabbit anti-Flag (Cell Signaling technology); 1:100 chicken anti-E.histolytica CSN5 (custom) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were first titrated on CD34+ fetal liver lysates and subsequently used at the following dilutions: Anti-GATA1 (Cell Signaling, 1:5), anti-STAG2 (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed thrice with PBS for 5 min at RT before incubation with the secondary antibody (1:200 rabbit anti-mouse AlexaFluor 488, Cell Signaling Technology) in antibody dilution buffer for 1 hour at RT ...
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bioRxiv - Cell Biology 2022Quote: ... except cells were cross-linked with formaldehyde (1%) for 5 min and cross-linked chromatin immunoprecipitated using an anti-HA rabbit monoclonal antibody (Cell Signaling Technology). Knock-in ESCs were first adapted to serum-free 2i/LIF condition as described before ChIP (15) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 minutes each at room temperature with agitation and then incubated with Anti-mouse IgG HRP-conjugated secondary antibody (Cell signaling 7076S) in 2% milk powder in PBST and incubated at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... or 5 million cells for histone modifications was sheared with SFX250 Sonifier (Branson) and immunoprecipitated with antibodies targeting H3K27ac (#8173, Cell signaling Technology), H3K4me1 (#5326 ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were diluted in either 5% goat serum/PBS-T or SignalStain Boost IHC Detection Reagent (8114S, Cell Signaling Technology (CST)) ...
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bioRxiv - Systems Biology 2021Quote: ... we washed the cells 5 X 5 min with 5% BSA-PBS and then incubated the cells at room temperature for 1 hour in 5% BSA-PBS containing 1:500 goat anti-rabbit secondary antibody conjugated to Alexa Fluor 594 (Cell Signaling, 8889). After the secondary incubation ...
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bioRxiv - Developmental Biology 2021Quote: ... coupled with the desired antibodies (5 ug each of H3K27me3 {Millipore 07-449}, H3K4me3 {Millipore 04-745} and H2AUb {Cell Signaling 8240S}) ...
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C with the following primary antibodies diluted 1:1000 in 5% BSA/TBST: anti-pro-IL-1β (#12703, Cell Signaling Technology) and anti-NLRP3 (AG-20B-0014-C100 ...
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bioRxiv - Cell Biology 2021Quote: ... blocked with 5% BSA/PBS (1 h at room temperature) and stained with the following primary antibodies to: vimentin (1:500; Cell Signaling, #5741), Ki67 (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... Magnetic beads were allowed to bind with 5 μg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) by incubating for 8 hours at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed in TBS-T (3 × 5 min) and probed with secondary antibodies: mouse (Cat #: 7076S; Cell Signaling Danvers, MA) or rabbit (Cat # ...
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bioRxiv - Biochemistry 2023Quote: ... the fixed cells were blocked in PBS containing 5% bovine serum albumin (BSA) and then incubated with mouse anti-HA antibody (6E2, Cell signaling Technology) with 1:800 dilution overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% skim milk for 1 hr and incubated overnight with one or more primary antibodies: phospho-Histone H3 (Cell Signaling, 3377S), α-tubulin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... + 5% milk and then incubated overnight at 4°C with the following primary antibodies in TBS+5% milk: anti-RPB1 (1:1000; Cell Signaling, 14958), anti-EZH2 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then cut and incubated with different primary antibodies diluted in 5% milk at 4°C overnight: αH3K27me3 (Cell Signaling C36B11) was used at 1:5000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Membranes were washed in TBST and incubated 1 hour at room temperature with secondary antibody diluted 1:5000 in 5% milk / TBST [anti-rabbit IgG HRP (Cell Signaling 7074S)] ...