Labshake search
Citations for Cell Signaling Technology :
101 - 150 of 10000+ citations for Septin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... The processed tissue was rinsed 3 times with PBS (20min each) to wash off the Dent’s Fix and then incubated with primary antibody in blocking buffer (pS6 Cell Signaling 2215, 1:1000) at 4°C with gentle rotation for 5 days ...
-
bioRxiv - Neuroscience 2023Quote: ... Normalization was carried out on the control group and related to the corresponding ß-actin (monoclonal mouse, Cat. No. #3700, dilution 1:2000 in blocking solution, Cell Signaling Technology and monoclonal rabbit ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were then immunoblotted over night at 4°C with the indicated primary antibodies diluted in blocking buffer: Phosphorylated Stat1 (Tyr701) (D4A7) (Cell Signaling, 1:1000), STAT1 (Cell Signaling ...
-
bioRxiv - Cell Biology 2020Quote: ... then blocked in 1X Animal-Free Blocking Solution (Cell Signaling Technologies, 15019) for 30min ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were then blocked with Animal-Free Blocking solution (Cell Signaling Technology) for 1h at room temperature ...
-
bioRxiv - Molecular Biology 2019Quote: ... Dry peptides were resuspended in 1x IAP buffer (Cell Signaling Technologies), clarified by centrifugation ...
-
bioRxiv - Cell Biology 2023Quote: Di-Glycine motif peptide identification was performed by Cell Signaling Technology, following UbiScan protocols and instructions (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2022Quote: ... Coverslips were blocked in 4% FBS in 1x PBS + 0.1% Triton X-100 prior to incubation with primary antibodies diluted in blocking buffer: rabbit anti-γ-H2AX (Cell Signaling, 2577, 1:400); mouse anti-p53 (Cell Signaling ...
-
bioRxiv - Cell Biology 2021Quote: ... MEFs were saturated with the blocking solution followed by a 1hr at 37°C incubation with the primary antibodies: Bcl-xL (Cell Signaling #2764, 1/1000) and IP3R C-terminal (abcam #ab190239 ...
-
bioRxiv - Neuroscience 2022Quote: ... incubated with blocking solution for 90min then with a combination of two primary antibodies: rabbit anti-Fos (1:2000; Cell Signaling Technology, RRID #2250) and chicken anti-mCherry (1:5000 ...
-
bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted with blocking solution and incubated with cells overnight at 4°C: Cleaved caspase-3 (rabbit, Cell Signaling #9661, 1:500), Anti-Nestin (mouse ...
-
bioRxiv - Physiology 2020Quote: ... The blots were washed and immunoreactive bands were detected by incubation with anti-rabbit HRP conjugated antibody (dilution 1:2000 in blocking solution; Cell signaling, Cat no. 7074S) for 1h at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... permeabilized with 0.5% Triton X-100 in blocking buffer for 15 min and incubated with primary antibodies against internal epitopes (DARPP32 (1:500, Cell Signaling Technology 2306, RRID:AB_823479) or PSD95 (1:500 ...
-
bioRxiv - Microbiology 2023Quote: ... followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500; Cell Signaling Technology, mAb #12830) or GAPDH (1:5000 ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were then incubated in the following primary antibodies diluted in blocking solution: anti-E-cadherin (24E10, CST 3195, Cell Signaling, dilution 1:200), anti-ezrin (E1281 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Slides were then stained with the appropriate primary antibody diluted in blocking solution and incubated overnight at 4°C: Phospho-Histone H3 (Cell Signaling, #9701, 1:200), Cleaved Caspase 3 (Cell Signaling ...
-
Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... Following blocking for 1h with 10% normal goat serum (NGS: Cell Signaling, Inc.), the primary antibody (Table 2) ...
-
bioRxiv - Immunology 2022Quote: ... The blocking solution is replaced with 250 μl of the Tomm20 (Cell Signaling) 1:500 diluted in 1% BSA and left to incubate for overnight at 4°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... Western blots were then blocked with LI-COR blocking buffer and incubated overnight with primary antibodies anti-pCREB (Ser133)(Cell Signaling 87G3, #9198, 1:1000) and anti-GAPDH (Cell Signaling ...
-
bioRxiv - Cell Biology 2022Quote: ... After blocking the membranes were incubated cold overnight with primary antibodies diluted in Intercept (TBS) Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000, β-catenin mAb #8480 Cell Signaling Technology 1:1000 ...
-
bioRxiv - Physiology 2023Quote: ... Membranes were blocked with Odyssey blocking buffer (LICOR, Omaha, NE, USA) and incubated overnight with an antibody against hypoxia inducible factor-1α (HIF-1α, Cell Signaling Technology #36169; 1:500). Proteins were visualized using IRDye 800CW secondary antibodies (LICOR ...
-
bioRxiv - Cancer Biology 2023Quote: ... The intestines were dissected and fixed in 4% paraformaldehyde for 1 h before blocking with 5% NGS and staining with rabbit-PH3 primary antibody (Cell Signaling Technology, Leiden, Netherlands, #3377) overnight ...
-
bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated for 2 h in a solution of TBST (0.05%) and LI-COR blocking buffer (5%) containing the following antibodies: anti-ERK1/2 (Cell Signaling Technologies; dilution factor: 1/2000) and streptavidin IRdye680 (LI-COR Biosciences ...
-
bioRxiv - Microbiology 2023Quote: ... Wells were washed three times and incubated with HRP-labeled anti-mouse IgG H+L (1:2500 dilution in blocking buffer) (Cell Signaling Technology, Danvers, MA, USA, #7076P2). Plates were developed with 1-Step ultra TMB-ELISA substrate solution and incubated at room temperature for 15-30 min ...
-
bioRxiv - Molecular Biology 2019Quote: ... Modified peptides were enriched using di-glycine-lysine antibody resin (Cell Signaling Technology). The enriched peptides were eluted with 0.15% trifluoroacetic acid in water ...
-
bioRxiv - Microbiology 2020Quote: ... Immunodetection was carried out by using rabbit anti-γ-H2A.X (SPQ) primary antibody (generated using peptide containing the SPQ motif, Cell signaling cat # 9718S, 1:1000), anti-H2A antibody (Abcam cat# ab88770 ...
-
bioRxiv - Genomics 2023Quote: ... Fixed and permeabilized cells were then blocked in 0.5% BSA and 0.2% Tween-20 in PBS (IF blocking buffer) for 30 minutes at room temperature and incubated with anti-Ngn2 (Cell Signaling Technology, cat# 13144; 1:250 dilution) and anti-MAP2 (Biolegend ...
-
bioRxiv - Systems Biology 2019Quote: Kac peptides were enriched using PTMScan® Motif antibody kits (Cell Signaling technology, Inc.) according to the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2023Quote: Acetylated peptides were enriched following the PTMScan kit (Cell Signaling Technologies, Danvers, MA, USA) protocol with some modifications ...
-
bioRxiv - Cancer Biology 2021Quote: ... followed by blocking and overnight incubation at 4°C with γH2AX primary antibody (Cell Signaling Technology). Cells were then incubated with species-specific goat secondary antibody coupled to AlexaFluor dye (568 ...
-
bioRxiv - Bioengineering 2023Quote: ... washed three times for 10 min in PBS and incubated for 1 h in blocking solution (5% normal goat serum, Cell Signaling Technology #5425, 0,3% Triton X-100 in PBS) at room temperature ...
-
bioRxiv - Physiology 2022Quote: Acetylated peptides from flash-frozen left ventricle were isolated the acetyl PTMScan Kit (Cell Signaling). Peptides were subject to LC-MS/MS ...
-
bioRxiv - Physiology 2023Quote: For DiGly peptide enrichment the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) (Cell Signaling) was used ...
-
bioRxiv - Cancer Biology 2023Quote: ... Phosphotyrosine-containing peptides were immunoprecipitated using PTMScan p-Tyr-1000 immunoaffinity beads (Cell Signaling #8803). Subsequently ...
-
bioRxiv - Cell Biology 2019Quote: ... Non-fat milk (5%) was used as a blocking solution and anti–mouse IgG (7076P2; Cell Signaling) and ECL Select (RPN2235 ...
-
bioRxiv - Biochemistry 2022Quote: ... and blocked with IHC-blocking buffer (IHC-PBS-T with 10% normal goat serum (Cell Signaling Technology)) for 1 h at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... resuspended in 200ul of blocking buffer and conjugated with 4ugr of anti-H2AK119ub antibody (Cell Signaling, 8240S) for 3h at 4C in a rotating platform ...
-
bioRxiv - Bioengineering 2023Quote: ... SARS-CoV-2 Spike RBD-ACE2 Blocking Antibody Detection ELISA Kit was obtained from Cell Signaling Technologies.
-
bioRxiv - Systems Biology 2022Quote: ... Peptides were then incubated with 4 µl of K-GG antibody bead-conjugate (Cell Signaling Technology, PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG ...
-
bioRxiv - Cancer Biology 2022Quote: ... TMT10 labeled Peptides with acetylated K were enriched with Acetyl-Lysine Motif kit (Cell Signaling Technology) following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Dry peptides were gently resuspended in 1.4 ml 1X immunoaffinity purification (IAP) buffer (Cell Signaling Technology) and cleared by centrifugation for 5 min at 10,000 ref at 4C ...
-
bioRxiv - Pathology 2023Quote: ... and then succinylated peptides were enriched using the PTMScan Succinyl-Lysine Motif Kit (Cell Signaling Technologies) as previously described but with slight adaptations (also see Supplemental Appendix 1 ...
-
bioRxiv - Immunology 2022Quote: ... Membranes were blocked with a blocking buffer of 5% non-fat milk powder (Cell Signaling Technology, Danvers, MA) in phosphate buffered saline with 0.05% tween-20 (PBS-T ...
-
bioRxiv - Systems Biology 2021Quote: ... The secondary antibodies were diluted in the blocking solution as follows: anti-mouse Alexa 647 (Cell signaling 4410S) 1/1000 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The tissue sections were then incubated with a blocking solution containing 5% goat serum (Cell Signaling Technology; 5425) at room temperature for 1 hour ...
-
bioRxiv - Biochemistry 2019Quote: ... Mono-methyarginine peptides were enriched by a PTMscan Mono-Methyl Arginine Motif [mme-RG] Kit (Cell Signaling) according to the manufacturer’s protocol followed by strong cation exchange (SCX ...
-
bioRxiv - Cancer Biology 2022Quote: ... the peptides were lyophilized and resuspended in immunoaffinity purification (IAP) buffer (PTMScan, Cell Signaling Technology, Beverly, MA). The supernatant containing pure peptides was centrifuged for 5 minutes at 10,000 × g at 4°C in a microcentrifuge ...
-
bioRxiv - Cell Biology 2023Quote: ... peptides were purified for the K-GG modification using the K-GG motif antibody kit (Cell Signaling). Eluted peptides were desalted and concentrated with PerfectPure C18 tips following with LC-MS/MS analysis ...
-
Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... Blocking was followed by overnight incubation at 4 ºC with rabbit anti-cleaved caspase 3 antibody (Cell Signaling 9661) at 1:400 dilution in antibody dilution buffer (1% BSA ...