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Citations for Cell Signaling Technology :
51 - 100 of 711 citations for Recombinant Sortase Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... HRP-tagged anti-rabbit and anti-mouse secondary antibodies (Cell Signaling Technology); Actin ...
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bioRxiv - Immunology 2023Quote: ... and recombinant murine MCSF (Cell Signaling Technologies, 5228) at 100 ng/mL for 2D culture and 200 ng/mL for 3D culture ...
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bioRxiv - Microbiology 2019Quote: ... using anti-His monoclonal antibody (Cell Signaling Technology, Inc., MA, USA). Similar protocol was followed for purification rRv1720cD5A and rRv1720cD94A.
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bioRxiv - Microbiology 2021Quote: ... Anti-His antibody was purchased from Cell Signaling Technology (Massachusetts, USA). Lipofectamine ...
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bioRxiv - Plant Biology 2023Quote: ... mouse α-His (Cell Signaling Technology; used in 1:1,000 dilution), rat α-HA (Hoffmann-La Roche AG ...
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bioRxiv - Immunology 2024Quote: ... and rabbit anti-His (#12698) were purchased from Cell Signaling Technology. The other antibodies used in this study included rabbit anti-SLFN11 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 µg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng/mL recombinant CXCL12 + U0126 (Cell Signaling Technology), or 100 ng/mL recombinant CXCL12 + LY294002 (Cell Signaling Technology) ...
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bioRxiv - Immunology 2024Quote: ... and recombinant mouse TGF-β (5231, Cell Signaling Technology) were purchased from the indicated sources.
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bioRxiv - Cell Biology 2020Quote: ... and anti-His tag clone D3I10 (rabbit polyclonal, Cell Signaling Technology 12698). The following secondary antibodies were used for microscopy ...
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bioRxiv - Cell Biology 2019Quote: ... The His-tag polyclonal rabbit antibody was from Cell Signaling Technology (# 2366P).
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Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factorbioRxiv - Microbiology 2021Quote: ... GP63 was detected by primary anti-his antibody (Cell Signaling Technology, 12698) with a secondary anti-rabbit fluorescent probe (Licor ...
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bioRxiv - Cancer Biology 2023Quote: ... then stained with anti-HIS tag secondary (Cell Signaling Technology, Cat# 14931S). Cells were washed with TIM-4 binding buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... or Rabbit His-Tag antibody (1:1000) (#12698, Cell Signaling Technology, USA)] and secondary antibody [Goat anti-mouse IgG conjugated with HRP (1:1000 ...
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bioRxiv - Pathology 2020Quote: ... Horseradish peroxidase-tagged secondary anti-rabbit IgG was from Cell Signaling (#7074, 1:2000).
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bioRxiv - Molecular Biology 2020Quote: ... or 100 ng/mL recombinant CXCL12 + LY294002 (Cell Signaling Technology). After 72 hours ...
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bioRxiv - Systems Biology 2020Quote: ... with dose titrations of recombinant mouse TNFα (Cell Signaling Technologies), 30μM Z-VAD(OMe)-FMK (Cayman Chemical) ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with an equal molar concentration of anti-His antibody (Cell Signaling), followed by incubation with a secondary antibody (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Blots were probed with polyclonal rabbit anti-His (0.4 μg/ml, Cell Signaling), followed by incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare ...
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bioRxiv - Physiology 2022Quote: ... followed by 1:10000 HRP-tagged anti-rabbit secondary antibody (Cell Signaling Technology, cat #7074). HRP signal was detected with chemiluminescent substrate (Pierce SuperSignal West Pico or Femto Plus ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies (Anti-FLAG: Sigma, F3165; Anti-His: Cell Signaling Technologies, Danver, MA, 2366S) were used at 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Rabbit monoclonal antibodies against 6-His-tags (12698S) were purchased from Cell Signaling Technology. The rabbit monoclonal anti-histone H2A ...
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bioRxiv - Biochemistry 2023Quote: ... detected with Alexa Fluor®488 Conjugated anti-His Antibody (Cell Signaling Technology, #14930), and finally the cell was detected by flow cytometry (BD ...
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bioRxiv - Cell Biology 2020Quote: ... GST tagged PTP-PEST was pulled down via immunoprecipitation using GST antibody (Cell Signaling Technology, USA). Immunoprecipitated proteins were loaded on to SDS-PAGE ...
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bioRxiv - Molecular Biology 2021Quote: Mouse recombinant IFN-gamma (Ifng) was purchased from Cell Signaling Technology (catalog #5222). The typical concentration used was 10 ng/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Human recombinant IFN-gamma (IFNG) was purchased from Cell Signaling Technology (catalog #8901). The concentration used was 100 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-HA tag and anti-His tag antibodies (Cell Signaling Technology Inc., Danvers, MA, United States).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the membrane was incubated with Rabbit His-Tag antibody (1:1000) (#12698, Cell Signaling Technology, USA) at 4 °C overnight and IRDye® 800CW Goat anti-Rabbit IgG (1:15000 ...
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated with goat anti-mouse or goat anti-rabbit antibody tagged with horseradish peroxidase (Cell Signaling) and immunoreactive bands were detected by enhanced chemiluminesence (GE Healthcare and Boston Bioproducts) ...
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bioRxiv - Plant Biology 2024Quote: ... Myc-tagged mCherry-PIF5 was detected using rabbit anti-Myc polyclonal antibodies (2272, Cell Signaling Technology, Danvers, MA) as the primary antibody at 1:100 dilution and donkey anti-rabbit AlexaFluor 555 antibodies (A31572 ...
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bioRxiv - Biophysics 2019Quote: ... cells were treated with recombinant human TGF-β1 (10 ng/ml, Cell Signaling Technology) 59 ...
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bioRxiv - Microbiology 2020Quote: ... Alexa flour 488 conjugated rabbit monoclonal His-tag was purchased from Cell Signaling Technology (Danvers, MA, USA). Fluorescein-conjugated goat anti-human IgG (#ZF-0308 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cell lysates were immunoprecipitated with the monoclonal anti-his-tag antibody (Cell Signaling Technology, Inc.; USA) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... followed by detection with 1μg/mL of AF647-conjugated secondary anti-HIS antibodies (Cell Signaling, Danvers, MA) for 30 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti-His tag antibody (catalog #: 2366S) was obtained from Cell Signaling Technology (Danvers, MA).
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bioRxiv - Developmental Biology 2020Quote: ... overnight in)and probed again with fluorescent-tagged goat IgG to rabbit IgG (1:500, Cell Signaling Technology; room temperature, 1 h), both in PBS supplemented with 1% BSA and 0.3% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with Alexa Fluor 488 or Alexa Fluor 555 tagged anti-mouse antibody (1:1000, Cell Signaling Technologies) for 1hr at RT in a humid black chamber ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then incubated with the recombinant pAG-MNase (Cell Signaling, 40366; 1:33 volume) in 150 µl Perm buffer per well for an hour at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... U-2 OS cells underwent treatment with 50 ng/mL recombinant IFN-γ (Cell Signaling). The cytokine was diluted in 0.5% (w/v ...
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bioRxiv - Bioengineering 2019Quote: ... and stained following standard protocols previously described (Xue et al. 2011) with primary antibodies against 3xHA-tagged Nme2Cas9 (anti-HA; Cell Signaling) and mCherry (anti-RFP ...
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bioRxiv - Cancer Biology 2022Quote: Cells with stable expression of FLAG-tagged human PDE6D were lysed in 1X cell lysis buffer (#9803, Cell Signaling, Danvers, MA) and incubated with anti-FLAG M2 Affinity Gel (A2220 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were sorted for the coexpression of each component via fluorescence-activated cell sorting on a FACS ARIA II (Beckton-Dickinson) by staining with appropriate fluorescently tagged anti-Myc and anti-Flag antibody for 30 minutes at 4°C (Cell Signaling Technologies) or expression of the transgenes ...
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bioRxiv - Neuroscience 2024Quote: ... the top five recombinant p-S65-Ub antibodies or the reference antibody (Cell Signaling Technology, 62802, E2J6T) were used as capturing agents ...
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bioRxiv - Physiology 2019Quote: ... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
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bioRxiv - Molecular Biology 2022Quote: Synchronized young adult animals expressing N-terminal HA-tagged PMK-1 were used for immunoprecipitation with anti-HA-Tag (C29F4) rabbit mAb (Cell Signaling Technology 11846) and eluted with 2x laemmli buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in 5% milk in PBST for 1 hour at room temperature and incubated overnight at 4°C with agitation in primary antibody at the following dilutions: rabbit anti-His (1:1,000, Cell Signaling, 2365), rabbit anti-GST (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA protein assay kit from Cell Signaling Technology. Thirty micrograms of total protein were mixed in a reducing sample buffer and used for mitochondrial apoptosis assay per the kit instruction ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μg/ml of recombinant antibodies and 0.2 μg/ml of the reference antibody (Cell Signaling Technology, 62802) were used ...
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bioRxiv - Cancer Biology 2021Quote: ... protein lysis buffer (Cell Signaling) containing a protease inhibitor cocktail was added and the mixture homogenized ...
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bioRxiv - Genomics 2020Quote: ... rabbit anti-GATA1 (NF that was produced against recombinant N-terminal zinc finger76, rabbit anti-GAPDH (14C10, Cell Signaling) and anti-β3 integrin (sc-14009 ...