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151 - 200 of 2311 citations for Recombinant Mouse CD8a Antigen Alpha Chain His tagged since 2019

• The Microtubule Severing Protein UNC-45A Counteracts the Microtubule Straightening Effects of Taxol

  Asumi Hoshino, et al., bioRxiv - Cell Biology 2023

  Quote: ... phospho-myosin light chain 2 (Ser 19) antibody (Cell signaling, #3671, 1:1000), and monoclonal anti-myosin clone MY-21 (Sigma ...
• RhoA activation promotes glucose uptake to elevate proliferation in MAPK inhibitor resistant melanoma cells

  Vasanth Siruvallur Murali, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Thr18/Ser19 Phospho-Myosin Light Chain 2 1:1000(Cell Signaling Technology, 3674), GLUT1 1:1000 (Cell Signaling Technology,12939S) ...
• Treatment with both TGF-β1 and PDGF-BB disrupts the stiffness-dependent myofibroblast differentiation of corneal keratocytes

  Krithika S. Iyer, et al., bioRxiv - Bioengineering 2024

  Quote: ... anti-phospho-myosin light chain (pMLC) (3671S; 1:200; Cell Signaling, Danvers, MA) and anti-vinculin (CP74 ...
• APP binds to the EGFR ligands HB-EGF and EGF, acting synergistically with EGF to promote ERK signaling and neuritogenesis

  Joana F. da Rocha, et al., bioRxiv - Neuroscience 2020

  Quote: ... Human recombinant EGF was from Cell Signaling and was used at a 100 ng/mL working concentration (WC) ...
• Suppression of sost/sclerostin and dickkopf-1 promote intervertebral disc structure in mice

  Tori Kroon, et al., bioRxiv - Bioengineering 2021

  Quote: ... and nuclear fractions to HRP-tagged Histone H3 (#12648, Cell Signaling). All blots were developed using Immobilon Luminata Forte (Sigma ...
• Pathogenic mutations in LRRK2 sequester Rab8a to damaged lysosomes and regulate transferrin-mediated iron uptake in microglia

  Adamantios Mamais, et al., bioRxiv - Cell Biology 2020

  Quote: ... FLAG-tagged proteins were eluted in 1x kinase buffer (Cell Signaling), containing 150 mM NaCl ...
• Blocking MIF secretion enhances CAR T-cell efficacy against neuroblastoma

  Josephine G. M. Strijker, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and CD19 CAR using PE-tagged protein L (58036S; Cell Signaling). B7-H3 CAR expression was determined using CD34-APC (FAB7227R ...
• Fibrotic human lung extracellular matrix as a disease-specific substrate for 3D in-vitro models of pulmonary fibrosis

  Igal Germanguz, et al., bioRxiv - Cell Biology 2019

  Quote: ... Immunohistochemical stains were performed for alpha smooth muscle actin (Cell Signaling Technology, 19245), fibrillin 2 (Sigma Life Science ...
• miR-329 and miR-495-mediated Prr7 downregulation is required for homeostatic synaptic depression in rat hippocampal neurons

  Michiko O. Inouye, et al., bioRxiv - Neuroscience 2022

  Quote: ... Rabbit monoclonal anti-alpha Tubulin (1:2000 dilution, 11H10 lot 112125S, Cell signaling). HRP-conjugated secondary antibodies Rabbit anti-Ms IgG H&L (402335 lot D00160409 ...
• Manganese-dependent microRNA trimming by 3’→5’ exonucleases generates 14-nucleotide or shorter tiny RNAs

  GeunYoung Sim, et al., bioRxiv - Molecular Biology 2022

  Quote: ... and/or anti-alpha-tubulin antibody (1:000 μg/mL, Cell Signaling Technology), and secondary antibodies ...
• New metabolic alterations and predictive marker pipecolic acid in sera for esophageal squamous cell carcinoma

  Lei Liu, et al., bioRxiv - Cancer Biology 2021

  Quote: ... including proliferating cell nuclear antigen (PCNA) (cat#2586S, Cell Signaling Technology, Boston, MA), H2A histone family member X (H2AX)(cat#7631S ...
• ATGs ubiquitination is required for circumsporozoite protein to subvert host innate immunity against malaria liver stage

  Hong Zheng, et al., bioRxiv - Microbiology 2021

  Quote: ... 1:1000 rabbit monoclonal anti-His-Tag (Cell Signaling Technology), 1:2000 mouse anti-β-actin (Sigma-Aldrich ...
• Unbiased interrogation of memory B cells from convalescent COVID-19 patients reveals a broad antiviral humoral response targeting SARS-CoV-2 antigens beyond the spike protein

  Jillian M. DiMuzio, et al., bioRxiv - Immunology 2021

  Quote: ... donor and AF488-labeled anti-HIS (Cell Signaling, Cat # 14930S) acceptor antibodies was used to screen patient-derived antibodies for their binding to recombinantly produced SARS-CoV-2 antigens ...
• The laminin-keratin link shields the nucleus from mechanical deformation and signalling

  Zanetta Kechagia, et al., bioRxiv - Cell Biology 2022

  Quote: ... rabbit Phospho-Myosin Light Chain 2 (Thr18/Ser19) 1:500 (Cell Signaling, Cat# 3674), rabbit anti-Cytokeratin 8 [EP1628Y] 1:2000 (Abcam ...
• Arp2/3 complex activity is necessary for mouse ESC differentiation, times formative pluripotency, and enables lineage specification

  Francesca M. Aloisio, Diane L. Barber, bioRxiv - Cell Biology 2021

  Quote: ... Phospho-Myosin Light Chain 2 Thr18/Ser19 E2J8F (1:1000; #95777; Cell Signaling Technology), ERK 1 C-16 (1:1000 ...
• α-actinin-4 drives invasiveness by regulating myosin IIB expression and myosin IIA localization

  Amlan Barai, et al., bioRxiv - Cell Biology 2021

  Quote: ... anti-myosin light chain 2 (phosphor- Ser19) rabbit monoclonal antibody (Cat #3671, Cell Signaling)) diluted in PBS overnight at 4°C ...
• Transient opening of tricellular vertices controls paracellular transport through the follicle epithelium during Drosophila oogenesis

  Jone Isasti-Sanchez, et al., bioRxiv - Developmental Biology 2020

  Quote: ... DSHB) and rabbit anti-phospho-Myosin light chain 2 (1:50; Cell Signaling Technology). Goat secondary antibodies were conjugated with Alexa Fluor 488 ...
• Dissecting the molecular basis of human interneuron migration in forebrain assembloids from Timothy syndrome

  Fikri Birey, et al., bioRxiv - Neuroscience 2021

  Quote: ... Phospho-Myosin Light Chain 2 (Ser19) antibody (1:200 dilution, rabbit, Cell Signaling, 3671S), anti-MAP2 antibody (1:10,000 dilution ...
• TGFβ2 regulates human trabecular meshwork cell contractility via ERK and ROCK pathways with distinct signaling crosstalk dependent on the culture substrate

  Haiyan Li, et al., bioRxiv - Cell Biology 2021

  Quote: ... or phospho-myosin light chain 2 (Ser19) (p-MLC [3675] 1:200; Cell Signaling), followed by incubation with Alexa Fluor® 488-conjugated or 594-conjugated secondary antibodies (Invitrogen ...
• Elimination Of Aberrantly Specified Cell Clones Is Independent Of Interfacial Myosin II Accumulation

  Olga Klipa, Fisun Hamaratoglu, bioRxiv - Developmental Biology 2021

  Quote: ... rabbit anti-phospho-Myosin light chain 2 (Ser19) (1:50, Cell Signaling Technology #3671), rabbit anti-Ap (1:1000 ...
• Preferential lipolysis of DGAT1 over DGAT2 generated triacylglycerol in Huh7 hepatocytes

  Rajakumar Selvaraj, et al., bioRxiv - Cell Biology 2021

  Quote: ... acyl-CoA synthetase long-chain family member 1 (ACSL1) (1:1000, Cell Signaling, #4047), perilipin 2 (PLIN2 ...
• Shear-induced phenotypic transformation of microglia in vitro

  Eunyoung Park, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-rabbit phosphorylated myosin light chain (1:50; 3671s, Cell signaling technology, MA, USA). After extensive washing with PBS ...
• E-cadherin-dependent phosphorylation of EGFR governs a homeostatic feedback loop controlling intercellular junction viscosity and collective migration modes

  Chaoyu Fu, et al., bioRxiv - Cell Biology 2023

  Quote: ... Phospho-Myosin Light Chain 2 (Ser19) antibody (3671, Cell Signaling Technologies, diluted 1:50) according to the manufacturer’s instructions ...
• Adenomatous polyposis coli (APC) regulates internalization and signaling of the chemorepellent receptor, Roundabout (ROBO) 1

  Yi-Wei Huang, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit monoclonal anti-clathrin heavy chain (D3C6) (#4796, Cell Signaling Technology, Danvers, MA, USA); mouse monoclonal anti-clathrin heavy chain (X22 ...
• Phase diversity-based wavefront sensing for fluorescence microscopy

  Courtney Johnson, et al., bioRxiv - Bioengineering 2024

  Quote: ... and Rabbit anti Phospho-Myosin Light Chain 2 (Thr18/Ser19) (Cell Signaling Technology, 3674), both at 1:200 dilution in PBS containing 1% BSA ...
• Suppression of sost/sclerostin and dickkopf-1 promote intervertebral disc structure in mice

  Tori Kroon, et al., bioRxiv - Bioengineering 2021

  Quote: ... and subsequently an HRP-tagged anti-Rabbit secondary antibody (#7074S, Cell Signaling). Whole cell lysates were normalized to HRP-tagged Actin antibody (#A3854 ...
• Macrophages sense ECM mechanics and growth factor availability through cytoskeletal remodeling to regulate their tissue repair program

  Matthew L. Meizlish, et al., bioRxiv - Immunology 2023

  Quote: ... and recombinant murine MCSF (Cell Signaling Technologies, 5228) at 100 ng/mL for 2D culture and 200 ng/mL for 3D culture ...
• AAV-mediated interneuron-specific gene replacement for Dravet syndrome

  John K. Mich, et al., bioRxiv - Neuroscience 2023

  Quote: ... and loading control rabbit polyclonal anti-alpha tubulin (1/5000 dilution, Cell Signaling #2144S) or mouse anti-alpha tubulin clone DM1A (1/5000 dilution ...
• ATP triggers macropinocytosis that internalizes and is regulated by PANX1

  Andrew K.J. Boyce, et al., bioRxiv - Cell Biology 2020

  Quote: ... The primary antibody used was early endosome antigen 1(EEA1 – 1:200; Cell Signaling) and the secondary antibody was Alexa647 AffiniPure donkey anti-rabbit IgG (1:600 ...
• Mutations in the juxtamembrane segment of the cholesterol-binding site of APP alter its processing and promotes production of shorter, less toxic Aβ peptides

  Linda Hanbouch, et al., bioRxiv - Neuroscience 2020

  Quote: ... The antibody directed against EEA1 (Early Endosome Antigen 1) was from Cell Signaling Technology. Goat anti rabbit IgG coupled to Alexa568 was from Life Technologies.
• Acquired Cross-resistance in Small Cell Lung Cancer due to Extrachromosomal DNA Amplification of MYC paralogs

  Shreoshi Pal Choudhuri, et al., bioRxiv - Cancer Biology 2023

  Quote: ... antigen retrieval was performed in SignalStain® Citrate Unmasking Solution (Cell Signaling, Cat# 14746S) at 98°C for 10 - 20 min ...
• Local delivery of cell surface-targeted immunocytokines programs systemic anti-tumor immunity

  Luciano Santollani, et al., bioRxiv - Immunology 2024

  Quote: ... Samples against intracellular antigens used as following: TCF1 (C63D9, Cell Signaling Technologies, 1:250), IFNg (XMG1.2 ...
• Surface-associated antigen induces B-cell permeabilization and lysosome exocytosis facilitating antigen uptake and presentation to T-cells

  Fernando Y. Maeda, et al., bioRxiv - Cell Biology 2021

  Quote: ... and incubated with rabbit anti-phosphorylated myosin light chain 2 (pMLC2) antibodies (Cell Signaling Technology) to label activated NMII (Bresnick 1999) ...
• Semaphorin3F Drives Dendritic Spine Pruning through Rho-GTPase Signaling

  Bryce W. Duncan, et al., bioRxiv - Neuroscience 2021

  Quote: ... For Myosin II rabbit polyclonal anti-Myosin II light chain (Cell Signaling Technology# 3672, RRID:AB_10692513) and rabbit polyclonal anti-phospho-Ser19 Myosin II light chain (Cell Signaling Technology# 3671 ...
• Semaphorin3F Drives Dendritic Spine Pruning through Rho-GTPase Signaling

  Bryce W. Duncan, et al., bioRxiv - Neuroscience 2021

  Quote: ... and rabbit polyclonal anti-phospho-Ser19 Myosin II light chain (Cell Signaling Technology# 3671, RRID:AB_330248) were used ...
• Rho activation drives luminal collapse and eversion in epithelial acini

  Vani Narayanan, et al., bioRxiv - Biophysics 2022

  Quote: ... rabbit monoclonal anti phospho-myosin light chain (Ser 19) (Cell signaling, working dilution 1:50) or rabbit monoclonal anti E-cadherin (24E10 ...
• AKT1-FOXO4 AXIS RECIPROACLLY REGULATES HEMOCHORIAL PLACENTATION

  Keisuke Kozai, et al., bioRxiv - Developmental Biology 2022

  Quote: ... serine palmitoyltransferase long chain base subunit 3 (LC3B, 1:1,000 dilution, 2775, Cell Signaling Technology), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
• Deletion of FAT1 in hybrid EMT cells stimulates the migration of neighboring non-mutant cells through secretion of extracellular vesicles

  Juliana Geay, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-phospho myosin light chain pMLC (Ser19, 1:200, 3671, polyclonal rabbit, Cell Signaling Technology), Vimentin (D21H3 ...
• Influence of myosin regulatory light chain and myosin light chain kinase on hair cells of the inner ear

  Ryohei Oya, et al., bioRxiv - Biophysics 2023

  Quote: ... anti-phospho-myosin light chain 2 (3671; Cell Signaling Technology, Danvers, MA, USA; 1:50); Alexa Fluor 488-labeled secondary antibody (Thermo Fisher Scientific ...
• Ca²⁺-driven cytoplasmic backflow secures spindle position in fertilized mouse eggs

  Takaya Totsuka, Miho Ohsugi, bioRxiv - Cell Biology 2024

  Quote: ... rabbit anti-phospho-myosin light chain 2 (Ser19) (pMLC) (1:100, Cell Signaling Technology #3671), for 2 hours ...
• Host cholesterol dependent activation of VapC12 toxin enriches persister population during Mycobacterium tuberculosis infection

  Amit Kumar Pandey, et al., bioRxiv - Microbiology 2019

  Quote: ... using anti-His monoclonal antibody (Cell Signaling Technology, Inc., MA, USA). Similar protocol was followed for purification rRv1720cD5A and rRv1720cD94A.
• PTEN differentially regulates endocytosis, migration, and proliferation in the enteric protozoan parasite Entamoeba histolytica

  Samia Kadri, et al., bioRxiv - Microbiology 2021

  Quote: ... Anti-His antibody was purchased from Cell Signaling Technology (Massachusetts, USA). Lipofectamine ...
• Schlafen-11 and -9 are innate immune sensors for intracellular single-stranded DNA

  Peng Zhang, et al., bioRxiv - Immunology 2024

  Quote: ... and rabbit anti-His (#12698) were purchased from Cell Signaling Technology. The other antibodies used in this study included rabbit anti-SLFN11 (Sigma ...
• Chronic dietary erythritol exposure elevates fasting plasma erythritol levels but does not cause weight gain or modify glucose homeostasis in mice

  Semira R. Ortiz, Martha S. Field, bioRxiv - Biochemistry 2021

  Quote: ... then incubated with primary antibodies (1:1000) against UCP1 and alpha-tubulin (Cell Signaling Technologies) for 1 hour at room temperature ...
• eIF6 rebinding dynamically couples ribosome maturation and translation

  Pekka Jaako, et al., bioRxiv - Cell Biology 2021

  Quote: ... recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 µg/mL ...
• Endothelial CXCL12 regulates neovascularization during tissue repair and tumor progression

  Zeshaan N. Maan, et al., bioRxiv - Molecular Biology 2020

  Quote: ... 100 ng/mL recombinant CXCL12 + U0126 (Cell Signaling Technology), or 100 ng/mL recombinant CXCL12 + LY294002 (Cell Signaling Technology) ...
• Single-cell transcriptomic atlas of the human substantia nigra in Parkinson’s disease

  Qian Wang, et al., bioRxiv - Neuroscience 2022

  Quote: ... Antigen retrieval was performed in SignalStain® Citrate Unmasking Solution (14746, Cell Signaling Technology, MA) with a pressure cooker (Presto Electronic ...
• Spatial integration of mechanical forces by alpha-actinin establishes actin network symmetry

  Fabrice Senger, et al., bioRxiv - Cell Biology 2019

  Quote: ... rabbit anti-phospho myosin light chain 2 (Ser19) (Cell Signaling Technology #3671; 1/50 for Iimmunofluorescence), rabbit anti-ACTN1 (Sigma #HPA006035 ...
• Mechanochemical control of epidermal stem cell divisions by B-plexins

  Chen Jiang, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit polyclonal anti-phospho-myosin light chain 2 (Ser19) (1:200, Cell Signaling, cat. no. #3671), rabbit polyclonal anti-phospho-myosin light chain 2 (Thr18/Ser19 ...
• MAP4K4 regulates biomechanical forces at adherens junctions and focal adhesions to promote collective cell migration

  Lara Elis Alberici Delsin, et al., bioRxiv - Cell Biology 2022

  Quote: ... rabbit polyclonal anti-phospho-Myosin Light Chain 2 (Ser19) at 1:1000 (Cell Signaling Technology, #3671), rabbit polyclonal anti-Non-Muscle Myosin HC II-A at 1:1000 (Biolegend ...