Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated citations for Cell Signaling Technology

Protein tyrosine phosphatase-PEST (PTP-PEST) mediates hypoxia-induced endothelial autophagy and angiogenesis through AMPK activation

Shivam Chandel, et al., bioRxiv - Cell Biology 2020

Quote: ... GST tagged PTP-PEST was pulled down via immunoprecipitation using GST antibody (Cell Signaling Technology, USA). Immunoprecipitated proteins were loaded on to SDS-PAGE ...

EGFR⁺ lung adenocarcinomas coopt alveolar macrophage metabolism and function to support EGFR signaling and growth

Alexandra Kuhlmann-Hogan, et al., bioRxiv - Immunology 2023

Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...

Interferon-gamma Induces Melanogenesis Via Post-Translational Modification of Tyrosinase

Xuan Mo, et al., bioRxiv - Molecular Biology 2021

Quote: Mouse recombinant IFN-gamma (Ifng) was purchased from Cell Signaling Technology (catalog #5222). The typical concentration used was 10 ng/ml ...

RNase L amplifies Interferon signaling by inducing PKR-mediated antiviral stress granules

Praveen Manivannan, et al., bioRxiv - Immunology 2020

Quote: ... Membranes were incubated with goat anti-mouse or goat anti-rabbit antibody tagged with horseradish peroxidase (Cell Signaling) and immunoreactive bands were detected by enhanced chemiluminesence (GE Healthcare and Boston Bioproducts) ...

Photobody formation spatially segregates two opposing phytochrome B signaling actions to titrate plant environmental responses

Ruth Jean Ae Kim, et al., bioRxiv - Plant Biology 2024

Quote: ... Myc-tagged mCherry-PIF5 was detected using rabbit anti-Myc polyclonal antibodies (2272, Cell Signaling Technology, Danvers, MA) as the primary antibody at 1:100 dilution and donkey anti-rabbit AlexaFluor 555 antibodies (A31572 ...

Epithelial protein lost in neoplasm (EPLIN)-β is a novel substrate of ornithine decarboxylase antizyme 1, mediating cellular migration

Dan Li, et al., bioRxiv - Cancer Biology 2022

Quote: ... anti-HA tag and anti-His tag antibodies (Cell Signaling Technology Inc., Danvers, MA, United States).

Forsythoside A blocks the infection of Avian Infectious Bronchitis Virus through binding S1 subunit

Ruiting Shen, et al., bioRxiv - Pharmacology and Toxicology 2023

Quote: ... the membrane was incubated with Rabbit His-Tag antibody (1:1000) (#12698, Cell Signaling Technology, USA) at 4 °C overnight and IRDye® 800CW Goat anti-Rabbit IgG (1:15000 ...

LPA/PKD-1 signaling promotes development of arteriolar niche that supports self-renewal of breast cancer stem cells and stemness

Yinan Jiang, et al., bioRxiv - Cancer Biology 2020

Quote: ... mouse anti-human/rat CD44 (5640S) and rabbit anti-human/mouse ALDH1A1 (12035S) (Cell Signaling Technology), rabbit anti-human/mouse CD36 (ab133625 ...

Loss of TIM4-Dependent Efferocytosis in Kupffer Cells Promotes Liver Fibrosis in Nonalcoholic Steatohepatitis

Hongxue Shi, et al., bioRxiv - Pathology 2024

Quote: ... human liver sections were incubated at 4 °C overnight with anti-human TIM4 (Cell signaling technology, #75484T ...

Multi-organ signaling mobilizes tumor-associated erythroid cells expressing immune checkpoint molecules

Yasuyo Sano, et al., bioRxiv - Cancer Biology 2020

Quote: ... human PDL1 (E1L3N; Cell Signaling Technology), and EF5 (ELK3-51 ...

A mosaic renal myeloid subtype with T-cell inhibitory and protumoral features is linked to immune escape and survival in clear cell renal cell cancer

Dorothee Brech, et al., bioRxiv - Immunology 2020

Quote: ... rabbit-anti-human FOXO1 (Cell Signaling) and mouse-anti-human CD3 (UCHT1 ...

Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins

Takeshi Uemura, et al., bioRxiv - Neuroscience 2020

Quote: ... or human IGF-I (Cell Signaling) was applied to the cultured CGCs at DIV2 ...

Antibody-induced glomerulonephritis is amplified by RTEC-intrinsic IL-17 signaling and restrained by IL-17-mediated induction of the endoribonuclease Regnase-1 (Zc3h12a)

De-Dong Li, et al., bioRxiv - Immunology 2021

Quote: ... human anti-Iκbζ (9244, Cell signaling), human/mouse anti-YY1 (sc-7341 ...

The Rhinolophus affinis bat ACE2 and multiple animal orthologs are functional receptors for bat coronavirus RaTG13 and SARS-CoV-2

Pei Li, et al., bioRxiv - Microbiology 2020

Quote: ... Alexa flour 488 conjugated rabbit monoclonal His-tag was purchased from Cell Signaling Technology (Danvers, MA, USA). Fluorescein-conjugated goat anti-human IgG (#ZF-0308 ...

FAP overexpression induce Epithelial-Mesenchymal Transition (EMT) in oral squamous cell carcinoma by down-regulating DPP9 gene

Qing-qing Wu, et al., bioRxiv - Molecular Biology 2019

Quote: ... the cell lysates were immunoprecipitated with the monoclonal anti-his-tag antibody (Cell Signaling Technology, Inc.; USA) and incubated overnight at 4°C ...

Neural Stem Cells Secreting Bispecific T Cell Engager to Induce Selective Anti-Glioma Activity

Katarzyna C. Pituch, et al., bioRxiv - Immunology 2020

Quote: ... followed by detection with 1μg/mL of AF647-conjugated secondary anti-HIS antibodies (Cell Signaling, Danvers, MA) for 30 min on ice ...

Hsp47 Promotes Biogenesis of Multi-subunit Neuroreceptors in the Endoplasmic Reticulum

Ya-Juan Wang, et al., bioRxiv - Cell Biology 2022

Quote: ... The mouse monoclonal anti-His tag antibody (catalog #: 2366S) was obtained from Cell Signaling Technology (Danvers, MA).

Transient EZH2 suppression by Tazemetostat during in vitro expansion maintains T cell stemness and improves adoptive T cell therapy

Yingqin Hou, et al., bioRxiv - Immunology 2023

Quote: ... and analyzed with the indicated primary antibodies (biotinylated anti-H3K27Me3 (1:1000, Cell Signaling Technology, Cat#4395S, clone: C36B11), anti-H3 (1:1000 ...

Autophagy mediates cancer cell resistance to doxorubicin induced by the Programmed Death 1/Programmed Death Ligand 1 immune checkpoint axis

Lori Minassian, et al., bioRxiv - Cancer Biology 2023

Quote: ... 30 minutes prior to addition of rPD-1-Fc (dosage according to Cell Signaling Technologies (CST) protocol ...

CDK5 Inhibitor Seliciclib Promotes Osteoblastic Differentiation of MSCs and Suppresses the Migration of MG-63 Osteosarcoma Cells

Hong Fu, et al., bioRxiv - Developmental Biology 2020

Quote: ... overnight in)and probed again with fluorescent-tagged goat IgG to rabbit IgG (1:500, Cell Signaling Technology; room temperature, 1 h), both in PBS supplemented with 1% BSA and 0.3% Triton X-100 ...

Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage

Johanna Zerbib, et al., bioRxiv - Cancer Biology 2023

Quote: ... cells were incubated with Alexa Fluor 488 or Alexa Fluor 555 tagged anti-mouse antibody (1:1000, Cell Signaling Technologies) for 1hr at RT in a humid black chamber ...

CUT&RUN detects distinct DNA footprints of RNA polymerase II near the transcription start sites

Michi Miura, Honglin Chen, bioRxiv - Molecular Biology 2020

Quote: ... Cells were then incubated with the recombinant pAG-MNase (Cell Signaling, 40366; 1:33 volume) in 150 µl Perm buffer per well for an hour at room temperature ...

Enhanced feature matching in single-cell proteomics characterizes response to IFN-γ and reveals co-existence of different cell states

Karl K. Krull, Syed A. Ali, Jeroen Krijgsveld, bioRxiv - Systems Biology 2024

Quote: ... U-2 OS cells underwent treatment with 50 ng/mL recombinant IFN-γ (Cell Signaling). The cytokine was diluted in 0.5% (w/v ...

pHLARE: A Genetically Encoded Ratiometric Lysosome pH Biosensor

Bradley A. Webb, et al., bioRxiv - Cell Biology 2020

Quote: ... and human LAMP1 (Cell Signaling Technologies; 9091) diluted in blocking buffer ...

ABCC1/MRP1 exports cGAMP and modulates cGAS-dependent immunity

Joanna H. Maltbaek, et al., bioRxiv - Immunology 2021

Quote: ... anti-mouse/human STING (Cell Signaling #13647), anti-human phospho-IRF3 (Abcam ab196035) ...

ABCC1/MRP1 exports cGAMP and modulates cGAS-dependent immunity

Joanna H. Maltbaek, et al., bioRxiv - Immunology 2021

Quote: ... anti-human phospho-STING (Cell Signaling #19781), anti-mouse phospho-STING (Cell Signaling #72971) ...

ABCC1/MRP1 exports cGAMP and modulates cGAS-dependent immunity

Joanna H. Maltbaek, et al., bioRxiv - Immunology 2021

Quote: ... anti-mouse/human Actin (Cell Signaling #3700), anti-human phospho-STING (Cell Signaling #19781) ...

S1P-S1PR1 activity controls VEGF-A signaling during lymphatic vessel development

AM Golding-Ochsenbein, et al., bioRxiv - Developmental Biology 2019

Quote: ... rabbit anti-human Rab5 (Cell Signaling, #3547P). After an intense wash step in 0.1% Triton-X PBS ...

Pumilio Proteins Exert Distinct Biological Functions and Multiple Modes of Post-Transcriptional Regulation in Embryonic Stem Cell Pluripotency and Early Embryogenesis

Katherine E. Uyhazi, et al., bioRxiv - Developmental Biology 2019

Quote: ... CDK4 monoclonal mouse anti-human (Cell Signaling), Cyclin D1 monoclonal mouse anti-human (Cell Signaling) ...

Nuclear soluble cGAS senses DNA virus infection

Yakun Wu, et al., bioRxiv - Immunology 2021

Quote: ... anti-human cGAS (Cell Signaling Technology, #79978), anti-mouse cGAS (Cell Signaling Technology ...

Splice switching oligonucleotide mediated gene knockdown in B cells and plasma cells

Anne Marchalot, et al., bioRxiv - Immunology 2020

Quote: ... rabbit anti-human RelA antibody (Cell Signaling) or mouse anti-beta-actin antibody (Sigma) ...

Bone morphogenetic protein 4 reduces global H3K4me3 to inhibit proliferation and promote differentiation of human neural stem cells

Sonali Nayak, et al., bioRxiv - Neuroscience 2020

Quote: ... Human OCT4 (Cell Signaling Technology, Cat#4641) and CCND1 (Cell Signaling Technology ...

Ataxin-3 links NOD2 and TLR2 mediated innate immune sensing and metabolism in myeloid cells

Thomas Chapman, et al., bioRxiv - Immunology 2019

Quote: ... rabbit anti-human p38 #9212 (Cell Signaling), rabbit anti-human LAMTOR1 #8975 D11H6 (Cell Signaling) ...

A base-line cellular antiviral state is maintained by cGAS and its most frequent naturally occurring variant rs610913