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Citations for Cell Signaling Technology :
151 - 196 of 196 citations for Recombinant Autographa Californica Multiple Nucleopolyhedrovirus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Total cell lysate was obtained by incubating treated and untreated DLD-1 cells in 1X RIPA (Cell Signaling Tech, Beverly MA), with phosphatase inhibitor cocktail and protease inhibitor tablets (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... The bead-immobilized GTP-bound RhoA and total RhoA in the lysates were probed by immunoblotting with anti-RhoA antibody (Cell Signaling).
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bioRxiv - Biochemistry 2020Quote: IP was performed on hepatocytes lysates using antibodies against LxRxx[S*/T*] and Rxx[S*/T*] phospho-motifs (both Cell Signaling) with Pierce Protein A/G Magnetic Beads according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were recovered following centrifugation and incubated overnight with 50 μL Streptavidin magnetic beads (Cell Signaling Technology, Danvers, MA, USA). Beads were rinsed using a magnetic stand at 25°C according to the following steps ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates for protein analysis were prepared by scraping cells in 1x Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA, USA) containing 1x protease/phosphatase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... For immunoprecipitation assays, cell lysates were incubated with Protein G Agarose (cat # 20398, Pierce) and anti-Flag antibody (cat # 2368, Cell Signaling) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Microbiology 2020Quote: ... 250 μL of lysate were incubated with 2 μL of mouse Myc-Tag antibody clone 9B11 (Cell Signaling Technology, Cat. 2276S) for 4 h with rotation at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Antibodies for detection of cell surface proteins by western blot of cell lysates were used as provided and were from the following sources: anti-ErbB3 (Cell Signaling, HER3/ErbB3 ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates were co-imunoprecipitated with anti-HA beads (HA-Tag C29F4 Rabbit mAb Sepharose® Bead Conjugate Cell Signaling #3956 RRID:AB_10695091) overnight at 4 °C under gentle agitation ...
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bioRxiv - Microbiology 2023Quote: ... The efficiency of siRNA knockdown was verified by immunoblotting of whole cell lysates with an anti-E-cadherin (24E10, Cell signaling) antibody.
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bioRxiv - Developmental Biology 2023Quote: ... aliquots of total cell lysate were subjected to SDS-PAGE and Western blot analysis with anti-phosphotyrosine (1:3000, Cell signaling) antibody ...
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bioRxiv - Microbiology 2023Quote: ... the cell lysate was subjected to SDS-PAGE and western blot analyses using anti-HA antibody (1:2,000, 2999S, Cell Signaling Technology).
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bioRxiv - Biochemistry 2024Quote: ... 10 mg of lysate representing one biological replicate was incubated for 2 hours at 4 °C with anti-Kv11.1 antibody (Cell Signaling #12889) covalently coupled to protein A/G magnetic beads (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit monoclonal anti-CREB1 1:2,000 diluted in the corresponding blocking solution (5% BSA-TBST) (Cat# 9197, RRID: AB_331277, immunogen: recombinant protein specific to the amino terminus of human CREB1 protein, Cell Signaling Technology, Danvers, MA, USA); rabbit affinity isolated anti-actin 1:3,500 diluted in PBST (Cat# A2066 ...
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bioRxiv - Neuroscience 2023Quote: ... penicillin/streptomycin and 2% Fetal bovine serum (for vehicle group) or the same media with 100ng/ml recombinant colony-stimulating factor 1 (Csf1, Cell Signaling, Boston, MA, USA). Csf1 stock was diluted in saline.
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bioRxiv - Cancer Biology 2020Quote: ... 5mg of lysate was incubated with 30ul of MyOne Streptavidin C1 Dynabeads or 5mg of Myc-Tag (9B11) Mouse mAb (Cell Signaling, #2276) antibody for 3 hours with subsequent 1-hour incubation with 30ul of Protein G Dynabeads for streptavidin pulldown and myc tag IP ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were first titrated on CD34+ fetal liver lysates and subsequently used at the following dilutions: Anti-GATA1 (Cell Signaling, 1:5), anti-STAG2 (Cell Signaling ...
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bioRxiv - Biophysics 2022Quote: ... Cellular protein tyrosine phosphorylation was performed by immunoblotting of total cell lysate (20 μg) using anti-pTyr antibody (4G10, a gift form Prof. Mangeat; CRBM) and anti-Actin (Cell Signaling Technology). Signal quantification and analyses were performed using ImageJ and GraphPad Prism respectively ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μl cell lysates served as a source of the E3 ligase CHIP (cells were lysed in Cell lysis buffer (9803, Cell Signaling Technology). After reactions ...
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bioRxiv - Genomics 2022Quote: ... acid-extracted histone lysate was prepared accordingly (Shechter et al., 2007) and subjected to western blotting using anti-H3K9ac (Cell Signaling, 9649), H3K14ac (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... the same amount of each cellular lysate was analyzed by gel electrophoresis and western blot using anti-WDR5 antibody (D9E1I; Cell Signaling #13105), anti-HSP90 antibody (F-8 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were immunoprecipitated from precleared lysates overnight at 4°C using 4 μg of control IgG (rabbit or mouse, Cell Signaling Technology) or indicated antibody ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were lysed in ODG buffer and the lysates were incubated with an anti-Myc-tag antibody (2276, Cell Signaling Technology). Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... the sonicated lysate was then separated to a new tube and primary antibodies was added (anti-H2AK119Ub Cell Signaling D27C, anti-H3K4me3 Cell Signaling CD42D8 ...
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bioRxiv - Bioengineering 2023Quote: ... Cell lysates were analysed by western blot with anti-Gli1 (L42B10) mouse monoclonal antibody (1:800, 2643, Cell Signaling Technology, RRID: AB_2294746) followed by goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP)-linked secondary antibody (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: Cleaved PARP in cell lysates was measured using PathScan® Cleaved PARP (Asp214) Sandwich ELISA Kit (Cell Signaling Technology, Danvers, MA, USA) as per manufacturer instructions ...
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MIIP downregulation promotes colorectal cancer progression via inducing adjacent adipocytes browningbioRxiv - Cancer Biology 2023Quote: ... Protein lysates containing 1 mg total protein were precleaned and precipitated with indicated antibody or IgG (Cell Signaling Technology, Danvers, MA, US) for 6 h at 4°C ...
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bioRxiv - Pathology 2023Quote: Western blot analysis was performed using whole liver lysates for HNF4α (Perseus Proteomics, PP-H1415-00) and cMyc (Cell Signaling Technology, 56055), as described previously [7] ...
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bioRxiv - Genomics 2024Quote: ... Cell lysate was centrifuged at 21,000 x g for 10 minutes and supernatant was mixed with Blue Loading Buffer (Cell Signaling Technology #7722) per the manufacturer’s recommended ratio ...
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bioRxiv - Genomics 2024Quote: ... The extent and kinetics of IKAROS1 degradation was determined by western blotting of cell lysates with an IKZF1 antibody (clone D10E5, Cell Signaling Technology).
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bioRxiv - Developmental Biology 2024Quote: MCF10A cells from 2D collagen stimulation assays were pelleted by centrifugation and lysed using 1X RIPA Buffer containing both 1x protease inhibitor cocktail and 1x phosphatase inhibitor Lysate was precleared with Protein-A Magnetic Beads (73778, Cell Signaling Technology).
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bioRxiv - Cancer Biology 2020Quote: ... 90% of the pre-cleared lysates were subsequently added to prewashed glutathione agarose beads from Active Ras Detection kit (Cell signaling Technology, #8821) for 1 hour at 4 °C under constant rocking ...
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bioRxiv - Neuroscience 2020Quote: ... were lysed in complete RIP lysis buffer and the cell lysates were divided into equal parts and incubated (overnight with rotation, at 4°C) with anti-Pol II (Cell Signaling Technology (CST), Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Aliquots of the cell lysates containing 50 μg of protein were submitted to SDS-PAGE and immunoblotted for TP53 (Cell Signaling Technology, #32532) and β-actin (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2019Quote: ... 80 μg of total cell lysates were analyzed by Western blot using the following antibodies and concentrations: LRP6 (1:1000, Cell Signaling Technology 3395), LRP5 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated internalized proteins in lysates were captured by NeutrAvidin beads (Pierce) and eluted by incubating in Blue Loading Buffer (50 mM DTT, Cell Signaling, Danvers, MA) containing 50 mM DTT at 95°C for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: Cleaved PARP as an apoptotic marker in cell lysates was measured using PathScan® Cleaved PARP (Asp214) Sandwich ELISA Kit (Cell Signaling Technology) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: Senescence-associated beta-galactosidase in HSVSMC lysates was quantified using the Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) (Cell signaling Technology). Measurement was done on cell lysates with the same total protein concentration ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole protein lysates were analyzed for total protein expression and lysine-methylated peptides were enriched using reagents from Cell Signaling Technology (catalogue no. 14809). In short ...
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bioRxiv - Cell Biology 2022Quote: Sorted MuSCs were cultured overnight in 96 well plates and cAMP measurements were performed on cell lysates using the Cyclic AMP XP® Assay Kit (cat Cyclic AMP XP® Assay Kit (cat # 4339, Cell Signaling Technology) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysates (20 µg) were subjected to SDS-PAGE and immunoblotted with specific antibodies against pFRS2a (#3864; Cell signaling Technology, Beverly, MA, USA), FRS2 (sc-17841 ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control antibody (Cell Signaling Technology, 1:300), or anti-IRF1 antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... 300 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control (Cell Signaling Technology, 1:300), anti-Ollas (Novus ...