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Citations for Cell Signaling Technology :
601 - 636 of 636 citations for Rat IL 18 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... 25 mg tumour tissue / IP were fixed in 1% formaldehyde (Applichem) and processed according to the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology).
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bioRxiv - Cancer Biology 2020Quote: ... Samples were alkylated and digested with trypsin at 37°C overnight and ubiquitinated peptides were enriched with PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology). After Sep Pak desalting ...
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bioRxiv - Cancer Biology 2020Quote: ... 90% of the pre-cleared lysates were subsequently added to prewashed glutathione agarose beads from Active Ras Detection kit (Cell signaling Technology, #8821) for 1 hour at 4 °C under constant rocking ...
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bioRxiv - Neuroscience 2021Quote: SA-β-gal staining was performed according to the protocol of the Senescence-β-Galactosidase Staining Kit (Cell Signaling Technology, Danvers, MA, USA). At least 200 cells were counted in each treatment group from three independent experiments.
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellet was then resuspended in 1 X PBS and cell fractionation was carried out using the Cell Fractionation Kit (Cell Signaling Technology, 9038) according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... The proportion of senescent cells was determined by staining for β-galactosidase activity using Senescence β-Galactosidase Staining Kit (9860, Cell Signaling Technology) as described42.
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bioRxiv - Cancer Biology 2022Quote: ... and fixed and stained for Senescence Associated β-Galactosidase Activity (SA-β-Gal) using the for Senescence β-Galactosidase Staining Kit (Cell Signaling) following manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... cells were stained with senescence-associated (SA)-β-galactosidase (gal) according to the manufacturer’s instructions for Senescence β-Galactosidase Staining Kit (Cell signaling, 9860). Cells were counterstained with DAPI ...
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bioRxiv - Cancer Biology 2022Quote: SUM159 and MDA-MB-231 cells were stained for SA-β-gal detection using a Senescence β-galactosidase Staining Kit (#9860, Cell Signaling). Staining was performed by following the manufacturer’s instructions and incubating the stained samples for 48hrs at 37°C without CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Frozen sections were hydrated with PBS for 10min at RT and stained using Senescence β-Galactosidase staining kit according to manufacturer’s instructions (Cell signaling technology, 9860). The sections were incubated at 37℃ in a dry incubator (no CO2 ...
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bioRxiv - Systems Biology 2024Quote: The enrichment of lysine acetylated peptides was performed using a PTMScan HS Acetyl-Lysine Motif [Ac-K] Kit (Cell Signaling Technology, Cat #50071) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated with an appropriate HRP-conjugated secondary antibody (Cell signalling technologies, cat. n. 7074) and LumiGLO® ECL kit (Cell Signaling Technology) was employed following manufacturer’s instruction before to exposure to X-ray film (CytivaTM) ...
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bioRxiv - Cell Biology 2023Quote: ... Cellular lysates from FLSs were harvested and a ChIP assay was performed using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (9005, Cell Signaling Technology). In brief ...
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bioRxiv - Molecular Biology 2024Quote: Senescence-associated beta-galactosidase in HSVSMC lysates was quantified using the Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) (Cell signaling Technology). Measurement was done on cell lysates with the same total protein concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... The rest of the eluate was dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology) as described in the manual ...
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bioRxiv - Genetics 2021Quote: ... and myotube (4 days differentiation in low serum differentiation medium) and ChIP was performed using the Simple ChIP Enzymatic Chromatin IP Kit (Cat# 9003; Cell Signaling Technology; Danvers, MA) following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2020Quote: ... stained with anti-F4/80-PE antibody (eBiosciences #12-4801-80) and isolated using an EasySep™ mouse PE positive selection kit that uses a magnetic bead-based purification strategy (Cell Signaling Technologies #17656) and in the presence of 2mM Icam4/av inhibitor peptide (Xue et al. ...
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bioRxiv - Neuroscience 2021Quote: Approximately 1.2 million microglia were exposed to the indicated treatments followed by cell preparation according to the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003). Samples were cross-linked for 10 min at room temperature with 1% formaldehyde solution ...
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bioRxiv - Cell Biology 2020Quote: ... Specificity of C12FDG detecting senescent cells was determined in comparison with results obtained with the senescence β-galactosidase staining kit (Cell Signaling Technology #9860) on IMR90 cells that were treated with 100 ng/mL doxorubicin (Sigma ...
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bioRxiv - Genetics 2019Quote: ChIP followed by allele-specific quantitative PCR (ChIP AS-qPCR) [38] were performed in U2OS cell line by using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Catalog# 9003) (Cell Signaling Technology, USA) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: Enrichment of K-ε-GG peptides was performed with the PTMScan ubiquitin remnant motif (K-ε-GG) kit (Cell Signaling Technology, cat. no. 5562) containing anti–K-ε-GG antibody cross-linked to protein A beads ...
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bioRxiv - Cancer Biology 2021Quote: ChIP was performed on 5X106 cells from 2-day control and radiated gliomaspheres using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, #9005S) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed and permeabilized with the Foxp3 transcription factor staining kit (eBioscience, 00-5523-00) prior intracellular staining with the indicated antibodies: H3K9ac (Cell Signaling, 9649, 1:3000), H3K27ac (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for malonylation with anti-malonyl antibody conjugated to agarose beads from the Malonyl-Lysine Motif Kit (Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The following steps were performed according to the manual of the PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling #59322). In brief ...
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bioRxiv - Cell Biology 2023Quote: SA-β-gal staining was performed using a SA-β-gal staining kit according to the manufacturer’s protocol (Cell Signaling Technology, Danvers, MA, USA). In short ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein was extracted with RIPA buffer and processed for acetylomic mass spectrometry as described previously55 using PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784).
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bioRxiv - Molecular Biology 2023Quote: ... Apoptotic cells were detected by TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining using in situ apoptosis detection kit (Takata Bio Inc., Shiga, Japan) or immunostaining of cleaved caspase-3 (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Microbiology 2023Quote: ... peptide samples resuspended in IAP buffer were incubated with 100uL of antibody-bead conjugates specific for acetyl-lysine (Cell Signaling Technologies, PTMScan kits #13416). Immunoprecipitation proceeded overnight at 4°C with gentle mixing ...
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bioRxiv - Cancer Biology 2023Quote: IMR90 cells seeded on the plate were fixed and stained for SA-β-gal activity with the senescence β-Galactosidase Staining Kit according to the protocol (Cell Signaling, #9860S).
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bioRxiv - Cancer Biology 2019Quote: ... cells were additionally fixed for 10 min in 1% formaldehyde (Applichem, Darmstadt, Germany) and then processed according to the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, Leiden, Netherlands). For chromatin preparation from tissue ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes were then collected by scraping and the CNMCS Compartmental Protein Extraction Kit (BioChain Cat# K3013010 Hayward, CA) with protease/phosphatase inhibitors (PI, Cell Signaling, Cat# 5872S, Danvers, MA) was used and the nuclear fractions were isolated for each treatment condition ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-GM130 (BD transduction Laboratories, ref:610822, 1:1000, rabbit ITGB1, ITGB4, ITGA4 (from integrin Ab Sampler kit, Cell signaling ref: 4749, 1:1000), rabbit anti-EGFR (Cell Signaling ref ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting DNA fragment samples were generated into libraries using the SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® (Cell Signaling Technology, 56795) and SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Dual Index Primers ...
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bioRxiv - Immunology 2024Quote: ... and kept at 4 °C for up to 10 days until chromatin fragmentation using SimpleChIP® Plus Sonication Chromatin IP Kit (Cell Signaling Technology, Danvers, MA). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Senescence-associated β-Galactosidase (SA β-gal) activity was measured in the hepatocytes using Senescence β-Galactosidase Staining Kit (Cell Signaling Technology-9860, Danvers, MA, USA) as per manufacturer’s instructions ...