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Citations for Cell Signaling Technology :
201 - 250 of 717 citations for QuantiFluo Acid Phosphatase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 0.5% SDS) supplemented with 1:100 protease/phosphatase inhibitor cocktail (Cell Signaling; catalog no. 5872S). Lysates were sheared by sonication (8 × 30s ...
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bioRxiv - Cell Biology 2021Quote: Chromatin immunoprecipitation assay was performed using SimpleChIP® Enzymatic Chromatin IP Kit - Magnetic Beads (Cell Signaling Technology, Inc., Danvers, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: CUT&RUN experiments were conducted using 200,000 PS or PSP cells using the CUT&RUN assay kit (Cell Signaling Technology, 86652). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: GTP-bound GTPase pull-down assays were performed using the Active Rac1/CDC42 Detection Kit (Cell Signaling Technology, Cat. #8815, 8819). After transfection or verteporfin treatment ...
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bioRxiv - Developmental Biology 2022Quote: ... The soft palatal tissue from E13.5 embryos was collected and fixed according to the manufacturer’s instructions based on Cut and Run assay kit (Cell Signaling, 86652). Ten μl of IgG isotype control (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: Senescence-associated β galactosidase assay was performed according to the manufacturer’s protocol and as described above (Cell Signaling Technology, Kit #9860). 5,000 cells were plated on each well of a 96-well dish ...
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bioRxiv - Genomics 2023Quote: CUT&RUN experiments were conducted using the CUT&RUN (Cleavage Under Targets & Release Using Nuclease) assay kit (Cell Signaling Technology, #86652) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: CUT&RUN (Cleavage Under Targets & Release Using Nuclease) libraries were prepared with the established CUT&RUN Assay Kit (Cell Signaling, #86652S). The following antibodies were used ...
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bioRxiv - Molecular Biology 2023Quote: The chromatin immunoprecipitation (ChIP) assay was performed with SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell signaling technology, #9005) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... 0.1% SDS) with 1X protease/phosphatase inhibitor cocktail (Cell Signaling Technology; Danvers, MA, USA; Cat# 5872) and homogenized with a Teflon-glass homogenizer ...
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bioRxiv - Immunology 2020Quote: ... Membranes were subsequently probed with an alkaline phosphatase conjugated goat anti-rabbit IgG (7054S, Cell Signaling) used at a dilution of 1:1000 in TBST containing 5% milk at room temperature for 2 hours ...
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bioRxiv - Physiology 2019Quote: ... homogenized in CER-I buffer containing a protease/phosphatase inhibitor cocktail (Cell Signaling Technology [CST], 5872) and centrifuged at ∼16,000 g ...
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bioRxiv - Neuroscience 2019Quote: ... 0.1% SDS) with 1X protease/phosphatase inhibitor cocktail (Cell Signaling Technology; Danvers, MA, USA; Cat# 5872). The retinas were then homogenized and incubated on ice for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% SDS) with 1X protease/phosphatase inhibitor cocktail (Cell Signaling Technology; Danvers, MA, USA; Cat# 5872) and homogenized with a Teflon-glass homogenizer ...
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bioRxiv - Cell Biology 2023Quote: ... 1-mM CaCL2 in H2 O) supplemented with 1:100 protease/phosphatase inhibitor cocktail (Cell Signaling). The protein content was measured using a Pierce BCA assay kit (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ChIP assays were performed in WT and Arp5-KO RD cells using the SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... quantified with Quantitative Colorimetric Peptide Assay (Thermo Fischer Scientific) and dried on Speed-Vacuum before enrichment with Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling). After Sep Pak desalting ...
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bioRxiv - Physiology 2020Quote: ChIP assays of frozen liver tissues of humanized mice were performed using a Simple ChIP Enzymatic Chromatin IP kit (Cell Signaling Technology) according to the manufacturer’s protocol ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: The chromatin immunoprecipitation (ChIP) assays was performed using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technologies, Inc., MA, US) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: The chromatin immunoprecipitation assay was performed as previously reported using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, Cat No. 9003)47 ...
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bioRxiv - Neuroscience 2024Quote: 200,000 primary NSCs either stimulated with IL1α (10ng/ml) or left unstimulated were used for CUT&RUN assay using the CUT&RUN kit from Cell Signaling Technologies® #86652 according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... freshly prepared by trypsin-EDTA digestion were used to perform CUT&RUN with the CUT&RUN Assay Kit (catalog no. 86652, Cell Signaling Technology), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Protein samples were collected and lysed using RIPA buffer containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysates were quantified by Bradford assay (Bio-rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... PANC-1 cells were lysed in buffer containing protease and phosphatase inhibitors (Cell Signaling, catalog number: 9803S). Total protein content was quantified by Bradford assay and equal amounts of proteins were analyzed in duplicate with arrays containing 4 to 6 spots for each of 60 probes (ACC058 ...
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bioRxiv - Cancer Biology 2020Quote: ... NP-40 buffer was supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling Technology, Danvers, MA, Cat# 5872S) and used to lyse frozen pellets ...
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bioRxiv - Molecular Biology 2022Quote: ... and associated proteins were harvested from top layer with RIPA buffer containing phosphatase inhibitor cocktail (Cell Signaling), and protease inhibitor cocktail (Sigma).
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bioRxiv - Systems Biology 2021Quote: ... We incubated the cell lysates with Phospho-YAP (Ser397) rabbit antibody and performed a sandwich-ELISA assay by using PathScan Phospho-YAP (Ser397) Sandwich ELISA Kit (Cell Signaling Technology, #57046). We then used a Synergy HTX Multi-Mode Reader (BioTek ...
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bioRxiv - Cell Biology 2023Quote: ... Cellular lysates from FLSs were harvested and a ChIP assay was performed using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (9005, Cell Signaling Technology). In brief ...
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bioRxiv - Molecular Biology 2024Quote: Senescence-associated beta-galactosidase in HSVSMC lysates was quantified using the Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) (Cell signaling Technology). Measurement was done on cell lysates with the same total protein concentration ...
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bioRxiv - Cell Biology 2020Quote: Senescence-associated β-galactosidase assays (Cell Signaling) were performed as per manufacturer’s instructions before manually counting positive cells Acidic organelles were detected using Lysotracker Deep Red (ThermoFisher ...
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bioRxiv - Genomics 2020Quote: ... Senescence-associated β-galactosidase assay (Cell Signaling) was performed according to the manufacturer’s instructions to evaluate the fraction of positively-stained cells at different passages ...
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bioRxiv - Genomics 2019Quote: Protein lysates were obtained using ice-cold 1X RIPA buffer containing protease and phosphatase inhibitors (Cell Signaling 9806), and freshly supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% w/v SDS and 90mM NaCl) supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology 5872S) and 100U/mL Benzonase (Sigma-Aldrich E1014) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with Protease and Phosphatase Inhibitor for assessment of total Stat 1(Cell Signaling Technology, Danvers, Massachusetts, USA) as well as phosphorylated-Stat 1 (Cell Signaling Technology ...
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bioRxiv - Physiology 2023Quote: ... using 20 μl/mg RIPA buffer supplemented with a protease/phosphatase inhibitor cocktail (Cell Signaling Technology, MA, USA). The homogenized tissue was centrifuged at 14,000rpm at 4°C for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: The EV pellet was resuspended in RIPA buffer with protease/phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA). Protein (20 µg ...
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bioRxiv - Cancer Biology 2023Quote: ... The following antibodies were used in the assay: anti-OCT4 antibody (15 μL/assay, #2750; Cell Signaling Technology, Danvers, MA) and Rabbit (DA1E ...
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bioRxiv - Bioengineering 2022Quote: ... Senescence-associated β-Galactosidase assay (Cell Signaling, #9860S) was performed and quantified as previously described ...
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bioRxiv - Cancer Biology 2020Quote: Cells treated for 48 h with NaB were lysed with a lysis solution buffer and acetylation of H3 was measured with PathScan® Acetylated Histone H3 Sandwich enzyme-linked immunosorbent assay (ELISA) Kit (Cell Signaling Technology, Danvers, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were prepared using RIPA buffer containing cOmplete ULTRA protease and phosphatase inhibitor (Cell Signaling Technology (CST), Danvers ...
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bioRxiv - Neuroscience 2023Quote: ... 10% sucrose, 0.8 M NaCl, 1 mM EDTA, 0.1% sarkosyl and 1X protease/phosphatase inhibitor cocktail, #5872, Cell signaling) in a 15 ml glass homogenizer with 15 up and down strokes at 70% power by hand on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue (n=3 tendon per sample) was homogenized in RIPA buffer (including 1X protease/phosphatase inhibitor, Cell Signaling #58725) to extract the total protein with the Biomasher II system and Powermasher II electric stirrer (Nippi Inc ...
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bioRxiv - Cell Biology 2023Quote: ... then cells were lysed in 100-200 μL RIPA buffer containing 1X protease-phosphatase inhibitor cocktail (Cell Signaling, #5872S), as previously described [95] ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5% w/v SDS and 90mM NaCl) supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology Cat#5872S) and 100 U/mL Benzonase (Sigma-Aldrich Cat#E1014).
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bioRxiv - Cell Biology 2024Quote: ... 0.5% w/v SDS and 90mM NaCl) supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology Cat#5872) and 100 U/mL Benzonase (Sigma-Aldrich Cat#E1014).
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bioRxiv - Neuroscience 2024Quote: ... CSF samples were measured using a pipette then supplemented with 1X protease/phosphatase inhibitor cocktail (Cell Signaling Technologies, #5872). CSF was stored at -80℃.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed once with 0.5% BSA/PBS and once with PBS prior to be resuspended in lysis buffer (50 mM Tris-HCl, pH 8.0, and 1% SDS) supplemented with protease/phosphatase inhibitor (Cell Signaling), and chromatin was solubilized by sonication in a Bioruptor (Diagenode ...
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bioRxiv - Cell Biology 2022Quote: ... isolated rat cardiomyocytes were lysed in RIPA buffer (Cayman #10010263) supplemented with protease and phosphatase Inhibitor cocktail (Cell Signaling #5872S) on ice for 1 hour ...
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bioRxiv - Immunology 2021Quote: The primary CD4+ T cells were lysed in the RIPA buffer with protease/phosphatase inhibitor cocktail (Cell Signaling Technology, Danvers, MA). After determining the protein concentration for each cell lysate ...
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bioRxiv - Cancer Biology 2021Quote: ... 100ug of protein was incubated with 6 uL of ubiquitin antibody and protease/phosphatase inhibitors (Cell Signaling Technologies, Danvers, MA, USA) overnight ...