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Citations for Cell Signaling Technology :
601 - 650 of 710 citations for Pyruvate Kinase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: SA-β-gal activity was detected in IMR-90 cells using a SA-β-gal staining kit (Cell Signaling, #9860). IMR-90 cells (40,000 cells/well in a 12-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... The BrdU assay was performed with the BD Accuri C6 flow cytometer using the APC-BrdU flow kit according to the manufacturer’s instructions (Cell Signaling).
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bioRxiv - Cell Biology 2020Quote: Cryo-embedded sections were stained using the senescence associated β-galactosidase (SA-β-Gal) staining kit (Cell Signaling Technology, 9860) as per the manufacturer’s instructions with the following modifications for tissue sections ...
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bioRxiv - Cancer Biology 2021Quote: ... RAS-GTP pulldown was performed according to the manufacturers’ instructions using the Active Ras Detection Kit (Cell Signaling Technology, #8821). In brief ...
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bioRxiv - Genetics 2022Quote: ... Chromatin from crosslinked cells was fractionated by digestion with micrococcal nuclease using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling) according to the manufacturer protocol ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... lysed and active RAS levels were measured by affinity purification using an Active Ras Detection Kit (Cell Signaling Technologies, 8821S). Pulldown samples were loaded on a 4-12% Bis-Tris NuPage Protein gel (NuPage ...
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bioRxiv - Systems Biology 2021Quote: ... K-□-GG remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-□-GG) Kit (Cell Signaling Technology). Crosslinking of antibodies to beads and subsequent immunopurification was performed with slight modifications as previously described62 ...
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bioRxiv - Cancer Biology 2020Quote: Senescence-associated-β-galactosidase (SA--β-gal) staining was performed using the Senescence β-Galactosidase Staining Kit (Cell Signaling, #9860) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: We subjected freshly fixed samples to β-galactosidase (β-gal) staining by using either β-gal staining kit (Cell Signaling), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Senescence was assessed using the Senescence β-Galactosidase Staining Kit at pH 6 following the manufacturer’s instructions (Cell Signaling Technology). For SAHF staining ...
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bioRxiv - Molecular Biology 2023Quote: ... Senescence-associated (SA) β-galactosidase activity was determined by using the Senescence SA-β-Galactosidase Staining Kit (Cell Signaling, #9860), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... DiGly peptide enrichment was performed using the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology (CST)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Immunoprecipitation was performed using a PAR-CLIP kit (Axl-Bio, China) and anti-HuR antibody (Cell Signaling Technology, USA, 12582S) according to the instructions ...
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bioRxiv - Immunology 2023Quote: ... Detection of pSTAT5 was performed using a PathScan® Phospho-Stat5 (pTyr694) Sandwich ELISA Kit (Cell Signaling Technology Cat# 7113). Detection of pSTAT6 was performed using a PathScan® Phospho-Stat6 (pTyr641 ...
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bioRxiv - Cancer Biology 2023Quote: ... β-galactosidase activity in cells was detected using the Senescence β-galactosidase staining kit (Cell Signaling Technology, Danvers, MA, USA) at pH 6.0 ...
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bioRxiv - Immunology 2023Quote: ... Detection of pSTAT3 was performed using a PathScan® Phospho-Stat3 (pTyr705) Sandwich ELISA Kit (Cell Signaling Technology Cat# 7300). Cell lysates were added to pre-coated ELISA wells and incubated at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... Detection of pSTAT6 was performed using a PathScan® Phospho-Stat6 (pTyr641) Sandwich ELISA Kit (Cell Signaling Technology Cat# 7275). Detection of pSTAT3 was performed using a PathScan® Phospho-Stat3 (pTyr705 ...
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bioRxiv - Cancer Biology 2023Quote: Senescence-associated β-galactosidase staining was performed using a senescence β-galactosidase staining kit (Cell Signaling Technology, cat. no. 9860) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Samples for ChIP-seq experiments were prepared using the SimpleChIP Plus Enzymatic chromatin IP kit (Cell Signaling Technology, Cat. 9005) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were subjected to enrichment of tyrosine phosphorylated proteins using an the PTMScan HS phopshotyrosine kit (Cell Signaling Technology, #38572) following in-solution tryptic digestion and desalting ...
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bioRxiv - Biochemistry 2023Quote: Kε-GG peptides were enriched using the PTMScan HS Ubiquitin/SUMO Remnant Motif (Kε-GG) Kit from Cell Signaling Technologies using the provided reagents and protocol ...
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bioRxiv - Neuroscience 2024Quote: ChIP-seq analysis from P35-42 mouse cortex was performed using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003) following manufacturer’s instructions with slight modifications ...
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bioRxiv - Cell Biology 2024Quote: ... Fixation and staining were performed using a β-galactosidase staining kit per the manufacturer’s instructions at pH 6.0 (Cell Signaling). Cells were counterstained with DAPI to enable quantification.
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bioRxiv - Developmental Biology 2020Quote: ... and incubated with annexin V and propidium iodide (PI) as per the manufacturer’s instructions (Early Apoptosis Detection Kit, Cell Signaling Technology). Cells were analyzed by flow cytometry using a BD FACSCanto cell analyzer (BD Biosciences) ...
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bioRxiv - Physiology 2022Quote: The acetyl peptide enrichment was performed on 3 mg of digested peptides using PTMscan Acetyl-Lysine Motif kit (Cell Signaling #13416). The peptides were mixed with 100 µL of 10x IP buffer (500 mM MOPS ...
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bioRxiv - Molecular Biology 2021Quote: ... SA-β-gal activity staining was performed at pH 6.0 (Kurz et al., 2000) using a commercial kit (Cell Signaling, #9860). To compare the intensity of SA-β-gal staining ...
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bioRxiv - Biochemistry 2020Quote: ... and measured IR phosphorylation by using the PathScan® Phospho-Insulin Receptor β (panTyr) Sandwich ELISA Kit (Cell Signaling Technology; #7082).
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bioRxiv - Systems Biology 2019Quote: ... and monomethyl arginine peptides were immunoprecipitated by addition of 40 uL of PTMScan Symmetric Di-Methyl Arginine Motif Kit (13563, Cell Signaling), PTMScan Asymmetric Di-Methyl Arginine Motif Kit (13474 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed on ice at 1000 cells/µL lysis buffer according to the manufacturer’s protocol of the active RAS detection kit (Cat.#8821; Cell Signaling Technology). Total protein concentrations of samples were measured using a BCA protein-assay kit (Cat.#23225 ...
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bioRxiv - Systems Biology 2020Quote: ... DiGly remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-ɛ-GG) Kit (Cell Signaling Technology (CST)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins samples were processed in SDS free lysis buffer for the assessment of Ras-GTP level using Active Ras Detection Kit (Cell Signaling) by following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Senescence-Associated β-galactosidase activity was assessed using a SA-β-galactosidase staining kit according to the manufacturer’s instructions (Cell Signaling). Positive cells were counted manually out of more than 100 cells total.
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bioRxiv - Cancer Biology 2021Quote: ... Detection of the HRP/ peroxidase enzyme was performed with SignalStain® DAB Substrate Kit from Cell Signaling (cat. no. 8059; www.cellsignal.com), per the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... Cleaved Caspase1 and cleaved Gasdermin D antibodies were purchased as a part of Pyroptosis Antibody Sampler Kit (#43811T, Cell Signaling Technology). After overnight primary incubation ...
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bioRxiv - Cancer Biology 2019Quote: MEFs were prepared from E13.5 embryos and senescence of MEFs was determined by using the Senescence β-Galactosidase Staining Kit (Cell Signaling) according to the manufacturer’s protocol57 ...
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bioRxiv - Cancer Biology 2020Quote: ... Ubiquitinated peptides were enriched by immunoaffinity purification using the PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology, 5562) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... ChIP was performed using an amount of BEL-ChIP nuclear extract corresponding to 3.4 μg of input genomic DNA in 250 μL of ChIP Buffer + 1X PIC (SimpleChIP Kit #9003, Cell Signaling Technology). Antibody was added according to the vendor’s specifications and incubated overnight with end-over-end mixing at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... All ChIP was performed using the SimpleChIP Enzymatic Chromatin IP kit with magnetic beads according to the manufacturer’s instructions (Cell Signaling Technologies). DNA levels were quantified by qPCR amplification using Power SYBR Green Mastermix (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... or WT N2aC plated in chamber slides using the Annexin V-FITC Early Apoptosis Detection Kit (Cell Signaling Technology, Danvers, MA). As a positive control for apoptosis and necrosis ...
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bioRxiv - Cancer Biology 2022Quote: β-Galactosidase staining at pH 6.0 was performed with 5 × 105 cells using the Senescence β-Galactosidase Staining kit (Cell Signaling # C10841) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: 25 mg cortical tissues were dissected from wild type mice (n = 3) and chromatin was prepared using the SimpleChIP plus Enzymatic Chromatin IP Kit (Cell Signaling). UBE3A-bound chromatin was immunoprecipitated using rabbit anti-UBE3A antibody (Bethyl Laboratories) ...
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bioRxiv - Developmental Biology 2023Quote: ChIP-qPCR and ChIP-seq samples were prepared following the instructions of the SampleChIPR Enzymatic Chromatin IP Kit (Cell Signaling, #9005). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Chromatin was sheared using a Covaris Sonicator for 20 minutes in sonication buffer (SimpleChIP Plus Sonication Kit, Cell Signaling Technology, #57976) to obtain fragments in the range of 200-500 bp ...
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bioRxiv - Cell Biology 2020Quote: K-ε-GG remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-Є-GG) Kit (Cell Signaling Technology). Crosslinking of antibodies to beads and subsequent immunopurification was performed with slight modifications as previously described [62] ...
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bioRxiv - Neuroscience 2021Quote: Antibodies on these sections included Rat anti-CD3 epsilon (1:100, NBP1-26685, Novus Bio) and Rabbit anti-C-Kit (1:100, D13A2, Cell Signaling Tech).
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bioRxiv - Neuroscience 2020Quote: ... 5 days after irradiation the plates were assayed for senescence via the Senescence β-galactosidase Cell Staining Kit (Cell Signaling, Catalog # 9860) and the staining was performed as per manufacturer’s protocol.
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bioRxiv - Plant Biology 2020Quote: ... Immuno-precipitation was performed for two replicates for each genotype (about 1 g tissue/IP) by SimpleChIP Plus Kit (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ChIP-seq experiments were performed with 108 HDFs per replicate using a SimpleChip Plus sonication chromatin IP kit (Cell Signaling Technology, 56383) according to the manufacturer directions ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti TRA-1-81 (1:200) and mouse anti-SSEA4 (1:200) (all from Cell Signaling Technology, StemLight Pluripotency Antibody Kit) to assess for pluripotency ...