Labshake search
Citations for Cell Signaling Technology :
101 - 150 of 302 citations for Protease Inhibitor Cocktail EDTA Free 100X in DMSO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... 0.1% SDS) with 1X protease/phosphatase inhibitor cocktail (Cell Signaling Technology; Danvers, MA, USA; Cat# 5872) and homogenized with a Teflon-glass homogenizer ...
-
bioRxiv - Cell Biology 2023Quote: ... 1-mM CaCL2 in H2 O) supplemented with 1:100 protease/phosphatase inhibitor cocktail (Cell Signaling). The protein content was measured using a Pierce BCA assay kit (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: ... phosphatase inhibitor cocktail (Cell Signaling), and protease inhibitor cocktail (Sigma ...
-
bioRxiv - Developmental Biology 2024Quote: MCF10A cells from 2D collagen stimulation assays were pelleted by centrifugation and lysed using 1X RIPA Buffer containing both 1x protease inhibitor cocktail and 1x phosphatase inhibitor Lysate was precleared with Protein-A Magnetic Beads (73778, Cell Signaling Technology).
-
bioRxiv - Genetics 2019Quote: ... and resuspended in a lysis buffer with the addition of complete protease inhibitor cocktail (Cell Signaling Technology). Chromatin was sheared by sonication 3×5 cycles of 30 sec ON/30 sec OFF with high intensity (Bioruptor ...
-
bioRxiv - Cancer Biology 2020Quote: ... NP-40 buffer was supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling Technology, Danvers, MA, Cat# 5872S) and used to lyse frozen pellets ...
-
bioRxiv - Microbiology 2022Quote: ... supplemented with protease inhibitor cocktail (PIC) at 1× (catalog number 5871; Cell Signaling Technologies, Inc., Danvers, MA) and stored at - 80°C ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.5% w/v SDS and 90mM NaCl) supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology 5872S) and 100U/mL Benzonase (Sigma-Aldrich E1014) ...
-
bioRxiv - Physiology 2023Quote: ... using 20 μl/mg RIPA buffer supplemented with a protease/phosphatase inhibitor cocktail (Cell Signaling Technology, MA, USA). The homogenized tissue was centrifuged at 14,000rpm at 4°C for 10 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1× Protease inhibitor cocktail and incubated with indicated antibodies (Supplementary Table 3) or normal rabbit IgG (Cell Signaling) at 4 degrees overnight on nutator ...
-
bioRxiv - Cell Biology 2023Quote: ... + Protease Inhibitor (Cell Signaling Technology) + Benzonase Nuclease (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2021Quote: ... containing protein inhibitor and phosphatase inhibitor cocktail (Cell signaling technology). Protein concentration was estimated by BCA protein assay (Pierce) ...
-
bioRxiv - Cell Biology 2023Quote: ... then cells were lysed in 100-200 μL RIPA buffer containing 1X protease-phosphatase inhibitor cocktail (Cell Signaling, #5872S), as previously described [95] ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.5% w/v SDS and 90mM NaCl) supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology Cat#5872S) and 100 U/mL Benzonase (Sigma-Aldrich Cat#E1014).
-
bioRxiv - Cell Biology 2024Quote: ... 0.5% w/v SDS and 90mM NaCl) supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology Cat#5872) and 100 U/mL Benzonase (Sigma-Aldrich Cat#E1014).
-
bioRxiv - Neuroscience 2024Quote: ... CSF samples were measured using a pipette then supplemented with 1X protease/phosphatase inhibitor cocktail (Cell Signaling Technologies, #5872). CSF was stored at -80℃.
-
bioRxiv - Biochemistry 2023Quote: ... and phosphatase inhibitor cocktail (Cell Signaling Technologies) on ice for 30 min ...
-
bioRxiv - Cell Biology 2022Quote: ... containing protease inhibitors (Cell Signaling Technology). Protein quantification of the lysed cells and exosome samples was performed using the bicinchoninic acid assay (BCA assay ...
-
bioRxiv - Cell Biology 2022Quote: ... isolated rat cardiomyocytes were lysed in RIPA buffer (Cayman #10010263) supplemented with protease and phosphatase Inhibitor cocktail (Cell Signaling #5872S) on ice for 1 hour ...
-
bioRxiv - Cancer Biology 2024Quote: Cells were harvested post-treatment and re-suspended in lysis buffer (50⍰mM Tris-HCl pH 7.4, 150⍰mM NaCl, 1% NP-40, 1% protease inhibitor cocktail (Cell Signaling), sonicated ...
-
bioRxiv - Immunology 2021Quote: The primary CD4+ T cells were lysed in the RIPA buffer with protease/phosphatase inhibitor cocktail (Cell Signaling Technology, Danvers, MA). After determining the protein concentration for each cell lysate ...
-
bioRxiv - Biochemistry 2022Quote: ... The cultures were thawed on ice water and 1 mM phenylmethylsulfonyl fluoride [PMSF] with 0.1x protease inhibitor cocktail (Cell Signaling Technology) were added ...
-
bioRxiv - Cancer Biology 2019Quote: ... plus phosphatase inhibitor cocktail (Cell Signaling Technology #5872S). Protein concentration was determined using Pierce BCA Protein Assay (Thermo Fisher Scientific 23225) ...
-
bioRxiv - Bioengineering 2023Quote: ... and 1x phosphatase inhibitor cocktail (Cell Signaling Technology). Cell lysates were centrifuged at 12,000 g for 10 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... containing protease inhibitor PMSF (Cell Signaling Technology). Total protein was extracted in sample buffer (Cell Signaling Technology) ...
-
bioRxiv - Cancer Biology 2022Quote: ... supplemented with Protease Inhibitor (Cell Signaling Technologies) and PhosStop (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2022Quote: ... containing protease/phosphatase inhibitors (Cell Signaling #5872). Triton X-100 was added to a final concentration of 1% ...
-
bioRxiv - Cell Biology 2022Quote: ... 1x protease and phosphatase inhibitor (Cell Signaling), and 250 units/ml benzonase nuclease (Sigma)) ...
-
bioRxiv - Cell Biology 2022Quote: ... 1x protease and phosphatase inhibitor (Cell Signaling), and 250 units/ml benzonase nuclease (Sigma)) ...
-
bioRxiv - Cancer Biology 2022Quote: Flash frozen liver chunks were weighed and lysed with RIPA buffer (Astral Scientific, Australia) containing protease and phosphatase inhibitors cocktail (Cell Signaling Technology) at a concentration of 10 μL of RIPA per mg of tissue ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse brain lysates or cell lysates (1-2 mg) with protease inhibitor cocktail were incubated with anti-MeCP2 (Cell Signaling Technology, 3456) or anti-OGT (Sigma-Aldrich ...
-
bioRxiv - Systems Biology 2019Quote: ... washed with 1 mL HBSS and lysed in 100 μL of 9 M urea containing protease and phosphatase inhibitor cocktail (Cell Signaling Technology). Samples were split into two 30 μg aliquots and separated on a Bolt 4-12% Bis-Tris Plus SDS gel (Cat ...
-
bioRxiv - Cell Biology 2020Quote: Cells were trypsinized and washed with PBS and then lysed for 30 minutes on a rotating wheel at +4 °C in RIPA buffer supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling, 5872). Lysates were sonicated with a Bioruptor Soni cation System (UCD200 ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.5% w/v sodium dodecyl sulfate (SDS) and 90mM NaCl) supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology Cat#5872) and 100 U/mL Benzonase (Sigma-Aldrich Cat#E1014) ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were collected by centrifugation at 500 × g for 5 minutes and resuspended in 1 ml TNE buffer (150 mM NaCl, 2 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (Cell Signaling Technology, Danvers, MA), passed through a 25 gauge needle to shear the cells ...
-
bioRxiv - Cancer Biology 2019Quote: ... and phosphatase inhibitor cocktail (Cell Signaling Technology, Cat. # 5870S). Tumor tissues were harvested and snap frozen in LN2 until ready to be lysed ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 1% Phosphatase Inhibitor Cocktail (Cell Signaling Cat# 5870). Lysates were sonicated at 4°C using the Bioruptor Sonicator (Diagenode) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and a phosphatase inhibitor cocktail (Cat# 5870; Cell Signaling) for the western blotting or immunoprecipitation ...
-
bioRxiv - Molecular Biology 2021Quote: ... with protease inhibitor (Cell signaling, Danvers, MA, USA). Lysates were cleared by centrifugation and supernatants were obtained ...
-
bioRxiv - Cell Biology 2020Quote: ... with protease/phosphatase inhibitor (5872, Cell Signaling, MA), and sonicated ...
-
bioRxiv - Microbiology 2019Quote: ... containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysate was incubated on ice one hour ...
-
bioRxiv - Neuroscience 2023Quote: ... containing protease/phosphatase inhibitors (Cell Signaling, Cat#5872). Triton X-100 (Sigma ...
-
bioRxiv - Cancer Biology 2023Quote: ... and phosphatase/protease inhibitor (Cell Signaling Technology, 5872S). Nuclei were resuspended in 10mm Tris-HCl ...
-
bioRxiv - Cell Biology 2024Quote: ... and protease/phosphatase inhibitors (Cell Signaling Technology, 5872). Cells were collected in 1x RIPA buffer containing protease/phosphatase inhibitors and incubated on ice for 30 minutes ...
-
bioRxiv - Cancer Biology 2020Quote: For WCM154 Western blots, cell pellets were lysed in RIPA buffer (MilliporeSigma, 20-188) supplemented with Protease/Phosphatase Inhibitor Cocktail (Cell Signaling Technology, 5872S). Protein concentrations were assayed with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... Then the cells were washed with cold phosphate-buffered saline (PBS) and lysed using RIPA buffer with protease inhibitor cocktail (#5871S, Cell Signaling, Danvers, MA) and phosphatase inhibitor cocktail (#78420 ...
-
bioRxiv - Genetics 2023Quote: MEFs were washed twice with cold 1x PBS and lysed using RIPA buffer with a protease/phosphatase inhibitor cocktail (Cell Signaling Technology 5872) to obtain total protein samples ...
-
bioRxiv - Developmental Biology 2019Quote: ... proteins were extracted from the heads of 30-50 wildtype and mdkami5001 embryos in cold RIPA lysis buffer containing protease and phosphatase inhibitor cocktail (Cell Signaling Tec., Danvers, MA). Proteins were separated in 12%Mini-PROTEIN TGX Precast gel (BioRad ...
-
Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... THP-1 macrophages were washed with 1X PBS and resuspended in cell extraction buffer supplemented with 1 mM PMSF and a protease inhibitor cocktail (Cell Signaling Technology (Danvers, MA). Nuclear and cytoplasmic protein fraction concentrations were quantified by the Bradford protein assay (Bio-Rad).
-
bioRxiv - Cancer Biology 2022Quote: The cells were harvested and lysed using radioimmunoprecipitation assay (RIPA) buffer supplemented with PMSF (1mM) and protease inhibitor cocktail (Cell Signaling Technology, Massachusetts, USA). The protein concentration was determined using Bradford protein assay (Sigma ...