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Citations for Cell Signaling Technology :
451 - 500 of 669 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... 0.1 % Tween-20) for 1 h and incubated overnight with phospho-S6 ribosomal protein antibody (#2211, Cell Signaling) in 1 % BSA TBST at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... and total proteins were extracted from the cells by adding Cell Lysis Buffer (Cell Signaling Technology, MA, USA) containing 500 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-phospho ribosomal protein S6 (Ser235/236; Cell Signaling Technology, catalog no. 4858, 1:1000 for WB), mouse anti-ribosomal protein S6 (Cell Signaling Technology ...
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bioRxiv - Microbiology 2023Quote: ... Expression of iKd PfGEXP15 protein was checked by Western blotting using anti-HA (1/1000, Cell signaling C29F4) followed by anti-Rabbit IgG (1/20000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein was run on an SDS PAGE and probed with anti-his antibody (Cat # 2365, Cell Signaling Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... along with 10 μl of pre-washed Protein-A Agarose beads (Cat. No. 9863S, Cell Signaling, Danvers, MA). The tubes were left on a rotating disc at 40C overnight for pre-clearing the lysates ...
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bioRxiv - Cancer Biology 2023Quote: ... each lysate was pre-cleared with 20 μL of pre-washed Protein A agarose beads (Cell Signaling 9863S). Next ...
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bioRxiv - Bioengineering 2020Quote: ... The gel was then rinsed briefly in deionized water before equilibrating in tris-buffered saline with Tween® (1X TBST, from Cell Signaling Technology 9997S 10X stock) overnight to remove any residual lysis buffer (exchanging the buffer after 2h) ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant mouse Ifng and human IFNG proteins (with carrier) were purchased from Cell Signaling Technology (catalog #39127 and 8901). They were reconstituted with sterile water at a concentration of 0.1 mg/ml ...
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bioRxiv - Genetics 2019Quote: ... as described previously.21 The following antibodies were used for western blotting: anti-S6 ribosomal protein (Cell Signaling Technology), anti-phospho-S6 ribosomal protein (Ser240/244 ...
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bioRxiv - Immunology 2019Quote: ... The following primary antibodies were used to probe protein levels by immunoblotting: anti-GAPDH (Cell Signaling Technology, clone D16H11), anti-β-Actin (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2020Quote: ... protein from transfected 293T cells was analyzed by western blotting with an anti-GFP antibody (clone D5.1, Cell Signaling). This corresponded with an increase in protein size from 27 to 32 kDa by western blotting (Fig S2C) ...
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bioRxiv - Immunology 2019Quote: ... 40 μg of sheared chromatin/sample was incubated with protein G magnetic beads with α-RelB (10544, Cell Signaling) or α-NF-κB2 antibodies (37359 Cell Signaling ...
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bioRxiv - Physiology 2019Quote: ... and equal amounts of protein were incubated overnight at 4 °C with rabbit acetyl-lysine antibody (AcK; Cell Signaling). Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology) ...
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bioRxiv - Biochemistry 2021Quote: Fibroblasts and podocytes were washed twice with PBS and proteins were extracted with the lysis buffer (Cell Signaling Technology) which protease and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto nitrocellulose and incubated with primary antibody: LC3 (rabbit polyclonal, Cell Signaling, cat # 12741S, 1:1000), SQSTM1/p62 (guinea pig polyclonal ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to PVDF transfer membranes and incubated with primary antibodies against pAKT serine 470 (Cell Signaling Technology) (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μg of nuclear protein lysates were incubated with 2 μg of either Myc-Tag antibody (Cell Signaling Technology) or an endogenous NUP210-specific antibody (Bethyl Laboratories) ...
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bioRxiv - Cancer Biology 2022Quote: ... S6-ribosomal protein (pS235/S236) (D57.2.2E) and SYK (pY525/Y526) (C87C1) were from Cell Signaling Technologies (Leiden, The Netherlands). Antibodies against Btk (pY223)/Itk (pY180 ...
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bioRxiv - Physiology 2022Quote: ... Protein lysates were incubated overnight at 4 °C with rabbit acetyl-lysine (Ac-K, #9441) from Cell Signaling Technology. Protein densitometry was measured using Image J software (National Institutes of Health ...
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bioRxiv - Immunology 2022Quote: ... and detection of indicated proteins was carried out using the following antibodies: Aiolos (clone D1C1E, Cell Signaling, 1:20,000), Bcl-6 (clone K112 ...
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bioRxiv - Plant Biology 2019Quote: ... Phosphorylated (activated) MAPK proteins were detected using anti-phospho-p44/42 MAPK T202/Y204 (pERK) antibodies (Cell Signaling; 9101). SlM3Kα proteins were detected using of anti-HA antibodies (Krackeler ...
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bioRxiv - Genetics 2020Quote: ... and Myc-tagged Rrp46 and EXOSC5 proteins were detected with anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling). As loading controls ...
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bioRxiv - Plant Biology 2021Quote: ... The proteins were separated by SDS-PAGE and detected by anti-phospho-p44/42 MAPK antibodies (Cell Signaling #4370) and anti-actin antibodies respectively.
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bioRxiv - Cancer Biology 2019Quote: ... Protein expression was analyzed by immunoblotting for expression of phospho-EGFR (Y1068, 1:1000, Cell Signaling, Danvers, MA, USA), total EGFR (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibody against PDZ Binding Kinase (PBK)/T-LAK cell-originated protein kinase (TOPK) was purchased from Cell Signaling Technology, Inc ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were detected with horseradish peroxidase (HRP)-linked anti-mouse IgG and anti-rabbit IgG secondary antibodies (Cell Signaling) and SuperSignal West Dura Extended Duration Substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... and anti-phosphorylated ribosomal protein S6 (p-rpS6240/244, 1:1500, rabbit polyclonal, Cell Signaling Technology Cat# 2215, RRID:AB_331682). Sections were then washed with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... INSR protein complexes were eluted from the beads by incubation in Blue Loading Buffer (50 mM DTT, Cell Signaling) at 95°C for 5 min.
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bioRxiv - Cell Biology 2021Quote: ... INSR protein complexes were eluted from the beads by incubation in Blue Loading Buffer (50 mM DTT, Cell Signaling) at 95°C for 5 min and were analyzed by standard western blot procedures ...
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bioRxiv - Microbiology 2022Quote: ... For protein detection the following primary antibodies were used at the indicated dilutions: GAPDH (Cell Signaling, #2118, 1:4000), γH2AX (Cell Signaling ...
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bioRxiv - Genetics 2022Quote: ... Protein lysates were incubated overnight at 4°C with mouse α-Myc antibody (1:400, [Cell Signaling Technologies, #2276]) on a rotisserie tube rotator ...
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bioRxiv - Cell Biology 2022Quote: ... MAFA and MAFB protein levels were normalized to endogenous β-actin by immunoblotting with anti-MAFA (Cell Signaling 79737), anti-MAFB (Cell Signaling 41019S ...
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bioRxiv - Cancer Biology 2022Quote: Protein samples were collected and lysed using a radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysates were quantified by Bradford assay (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: ... AtRsmD-4×Myc fusion proteins were detected using a mouse anti-Myc monoclonal antibody (1:10,000; Cell Signaling Technology).
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bioRxiv - Neuroscience 2022Quote: ... The lysate was centrifuged at 12 000 g for 15 min and the supernatant with 0.25 mg total protein was incubated with 0.5 μg anti-Ire1α (Cell Signaling) antibody for 4 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The levels of HuR protein were detected using western blot and anti-HuR antibody (Cell Signaling Technology, USA, 12582S). The probes used are listed in the Supplementary Table 6.
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bioRxiv - Microbiology 2023Quote: ... The extent of protein knockdown was determined by immunoblotting with an anti-c-Met antibody (#8198, Cell Signaling Technology), anti-E-cadherin antibody (#3195 ...
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bioRxiv - Microbiology 2022Quote: ... For protein detection the following primary antibodies were used at the indicated dilutions: AKT (Cell Signaling, #9272, 1:1000), phospho-AKT T308 (Cell Signaling ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred to supported nitrocellulose membrane and probed with p44/42 MAPK antibody from Cell Signaling Technology (CST) (9102 ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg of protein were added to pre-washed 50 μL streptavidin Sepharose bead conjugates (Cell Signaling Technology, 3419S) and rocked overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: GABARAP/Atg8a (1:1,000, E1J4E, Cell Signaling), Arl8 (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred on a PVDF membrane and incubated with the following antibodies: p-ERK (#9101, Cell Signaling Technology), total ERK (#9102 ...
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bioRxiv - Neuroscience 2024Quote: ... Equal protein loading of blots was confirmed by immunoblotting of GAPDH (rabbit monoclonal, #2118, Cell Signaling Technology, Danvers, USA), except β-tubulin (mouse monoclonal ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membranes (Prometheus) and probed with phospho-ROS1 Y2274 (3078; 1:1,000; Cell Signaling Technology), ROS1 (D4D6 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred to a 0.2um nitrocellulose membrane and stained with primary antibodies including BMI1 (6964S, Cell Signaling Technology), H4 (13919 ...
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bioRxiv - Genomics 2024Quote: ... nuclei was cleared by centrifugation and subjected to pre-clearance by incubating with Protein A agarose beads (Cell Signaling). Pre-cleared cell lysates were separately incubated with CTCF or non-specific IgG antibodies (Cell Signaling ...
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bioRxiv - Genetics 2021Quote: ... and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution) (Cell Signaling Technologies, cat. number D7D3). Additional blots were probed with rat anti-MRP5 NC3 monoclonal antibody (1:200 ...
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bioRxiv - Neuroscience 2021Quote: Soluble protein was collected from cell pellets and human brain tissue by resuspension in Cell Lysis Buffer (Cell Signaling Technologies) supplemented with 10µM PMSF on ice ...
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bioRxiv - Cell Biology 2022Quote: Primary antibodies used are listed here: Rabbit anti-Phospho-S6 Ribosomal Protein (Ser235/236) (1:1000, Cell Signaling, cat. #2211), Rabbit S6 Ribosomal Protein (5G10 ...