Labshake search
Citations for Cell Signaling Technology :
551 - 600 of 790 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2019Quote: ... — 10 mg of digested proteins were dissolved in 1X immunoprecipitation buffer (50 mM MOPS, 10 mM Na2HPO4, 50 mM NaCl, pH 7.2, Cell Signaling). Modified symmetric dimethyl arginine peptides ...
-
bioRxiv - Cancer Biology 2020Quote: ... Samples were eluted by precipitating beads on a magnetic rack and resuspending in 40 μL of 3× blue sample buffer 30× reducing agent: 1.25 M DTT (1:30; 7722s, Cell Signaling). Resulting samples were heated at 95°C (5 min) ...
-
bioRxiv - Neuroscience 2019Quote: ... A pellet from a 35mm dish was lysated in 100ul of lysis buffer consisting of RIPA 1x (#9806S, Cell signaling) and cOmplete protease inhibitors 1x (#11697498001 ...
-
bioRxiv - Biochemistry 2020Quote: ... at 1:1,000 dilution in blocking buffer or glucose 6-phosphate dehydrogenase (G6PDH) antibodies (8866, Cell Signaling Technology, 1:1,000). Immunoreactive species were detected with antibodies conjugated with horseradish peroxidase (715-035-150 ...
-
bioRxiv - Neuroscience 2021Quote: Brains were homogenized using Qiagen Tissue-Lyser II (28 Hz, 3 min, 3 times) in lysis buffer (Cell Signaling #9803) containing protease inhibitor cocktail (Roche #4693159001 ...
-
bioRxiv - Microbiology 2021Quote: Kinase assay was performed using recombinant proteins purified from E.coli and commercially available 10X Kinase buffer (Cell Signaling Technology, #9802). Recombinant proteins (N’-His PINK1 and N’-His Ajuba ...
-
bioRxiv - Cancer Biology 2021Quote: ... Membranes were incubated overnight in primary antibody diluted in blocking buffer (1:1000 anti-cleaved caspase-3 Cell Signaling Technologies #9664 ...
-
bioRxiv - Immunology 2022Quote: ... Cells were washed with Perm/Wash buffer twice before stained with rabbit anti-Cleaved Caspase 3 (Asp175) (Cell Signaling Technologies) at a 1:200 dilution for 30 mins at room temperature and was detected with anti-rabbit BV421 (Dnk ...
-
bioRxiv - Immunology 2020Quote: Cells were collected and lysed on ice for 30 min in 1X radioimmunoprecipitation assay (RIPA) buffer plus (Cell Signaling Technology) with protease and phosphatase inhibitor cocktail (Thermo Scientific) ...
-
bioRxiv - Physiology 2019Quote: Bits of frozen inguinal adipose pads were weighed and re-suspended in 1X cell lysis buffer (Cell Signaling Technology 9803) with protease (Roche 4693132001 ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 μg of fusion proteins and 8 μg of FLAG-PKD1-CA were incubated in kinase buffer (Cell Signaling Technology) for 30 min at 30°C in the presence of 200 μM ATP ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were washed once with 0.5% BSA/PBS and once with PBS prior to be resuspended in lysis buffer (50 mM Tris-HCl, pH 8.0, and 1% SDS) supplemented with protease/phosphatase inhibitor (Cell Signaling), and chromatin was solubilized by sonication in a Bioruptor (Diagenode ...
-
bioRxiv - Neuroscience 2021Quote: ... Slices from each individual animal were combined in separate pools and homogenized on ice in RIPA buffer (Cell Signaling Technology) containing 1% Halt™ protease and phosphatase inhibitor cocktail (Thermo Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... the treated hepatocytes were harvested through cell scraping and lysed by using the 1x cell lysis buffer (Cell Signaling Technologies) supplemented with 1x Halt Protease and Phosphatase inhibitor Cocktail (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Bound PRKAR1a or GSK3b were detected by incubation with rabbit anti- PRKAR1a antibody (Cell Signaling; 1:2000 in blocking buffer) or mouse anti-GSK3b antibody (Millipore ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cell pellets were washed with ice cold PBS and resuspended in 1X Annexin V binding buffer (Cell Signaling Technology, USA). Cells were then incubated with Annexin V-FITC conjugate (Cell Signaling Technology ...
-
bioRxiv - Molecular Biology 2022Quote: ... BEAS-2B cells were washed with ice-cold PBS and lysed with 1 X Cell Lysis Buffer (Cell Signaling Technologies) supplemented with fresh protease inhibitor cocktail (Cell Signaling Technologies) ...
-
bioRxiv - Bioengineering 2022Quote: ... and lysed by repetitive freezing-thawing cycles in NP-40 buffer (150 mM NaCl, 1.0% NP-40, 50 mM Tris, pH 8.0) supplemented with protease inhibitors (Cell Signaling Technology). ALP activity in cell lysates was measured using p-nitrophenyl phosphate (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2022Quote: ... and blocked in blocking buffer before incubation with 1:500 dilution of rabbit anti-HA primary (3725; Cell Signaling Technology) for 1 h at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... Whole cell extracts were isolated using RIPA lysis buffer (25mM Tris HCl pH 7.6, 150mM NaCl, 1% NP 40, 1% sodium deoxychlorate, 0,1% SDS; Cell Signaling Technology) supplemented with DNase ...
-
bioRxiv - Neuroscience 2022Quote: ... DNA fragments were released by shaking incubation and isolated using DNA Purification Buffers and Spin Columns (Cell Signaling Technology #14209). Yeast spike-in DNA (5ng ...
-
bioRxiv - Cell Biology 2022Quote: ... isolated rat cardiomyocytes were lysed in RIPA buffer (Cayman #10010263) supplemented with protease and phosphatase Inhibitor cocktail (Cell Signaling #5872S) on ice for 1 hour ...
-
bioRxiv - Genetics 2023Quote: ... Protein was extracted from frozen tissue by homogenization of tissue with RIPA lysis buffer and PMSF (Cell Signaling, Danvers, MA). Protein concentrations of samples were determined using Bradford Assay (Bio-Rad ...
-
bioRxiv - Biophysics 2023Quote: ... blots were incubated in blocking buffer (5%(w/v) milk powder (nonfat dry milk, Cell Signaling Technology, Danvers, MA, USA)) in TBST containing 25 mM Tris base ...
-
bioRxiv - Immunology 2023Quote: Protein lysates from FACS-sorted cDCs from control and Kdm5cΔItgax or Kdm5cΔZbtb46 spleens were prepared using CHAPS lysis buffer (Cell Signaling, 9852S) with protease inhibitors (Roche ...
-
bioRxiv - Microbiology 2023Quote: Cell lysis was performed using Radioimmunoprecipitation (RIP) assay buffer (#9806, Cell Signaling Technology, 3 Trask Lane, Danvers, MA 01923, USA) supplemented with protease and phosphatase inhibitor (#5872) ...
-
bioRxiv - Physiology 2023Quote: ... Blotted membranes were incubated overnight at 4°C in SuperBlock(PBS) Blacking buffer (pH 7.4) containing KathonTM Anti-microbial Agent with anti-mTOR (1:1000; Cell signaling), anti-p-mTOR (1:1000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... were washed 3 times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 20μL EZH2 antibody (anti-EZH2, Cell Signaling #5246) or 20μg IgG (anti-IgG ...
-
bioRxiv - Cell Biology 2023Quote: ... The reaction was stopped by directly replacing the culture media with 100 µL ice-cold RIPA buffer (Cell Signaling, 9806S) supplemented with cOmplete protease inhibitor (1× ...
-
bioRxiv - Developmental Biology 2023Quote: ... cells were stained with primary antibodies overnight at 4°C in a dilution buffer (PBS/ 0.01%BSA/ 0.3% Triton): YAP (1:100, D8H1X #14074 Cell Signaling Technology), TAZ (1:100 ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: Frozen tissue punches containing the insula were manually homogenized in ice cold 1X RIPA buffer (Cell Signaling Technology, Danvers, MA) containing 1X Halt protease inhibitor cocktail (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... Beads were resuspended in 500µL blocking buffer and mixed with 7.5µg antibody (NUT monoclonal rabbit antibody (Cell Signaling Technology, #3625S), or H3K27Ac monoclonal rabbit antibody (Abcam ...
-
bioRxiv - Cell Biology 2023Quote: The isolated UMOD-positive or -negative epithelium and stable HEK 293 cells were lysed by RIPA buffer (Cell signaling CAT9806) containing protease and phosphatase inhibitor cocktails (Roche ...
-
bioRxiv - Neuroscience 2023Quote: ... Mice were sacrificed 6 days after noise exposure and whole cochlear lysates were prepared in lysis buffer (9803; Cell Signaling) after adding protease (cOmplete ULTRA Tablets 05892791001 ...
-
bioRxiv - Molecular Biology 2023Quote: Primary antibodies were diluted 1:1000 in blocking buffer and included rabbit α-TOMM20 (clone D8T4N, Cell Signaling Technologies #4240), mouse α-MT-CO1 (clone 1D6E1A8 ...
-
bioRxiv - Physiology 2024Quote: ... Quadriceps muscles were mechanically ground by Mortar and Pestle in dry ice and homogenized in RIPA buffer (Cell Signaling, #9806), with the addition of PMSF (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: Cells were harvested post-treatment and re-suspended in lysis buffer (50⍰mM Tris-HCl pH 7.4, 150⍰mM NaCl, 1% NP-40, 1% protease inhibitor cocktail (Cell Signaling), sonicated ...
-
bioRxiv - Developmental Biology 2024Quote: ... Primary and secondary antibodies were diluted in blocking buffer and used 1:100 for anti-FoxO1 (rabbit; Cell Signaling, 2880), 1:100 for anti-ESRRB (mouse ...
-
bioRxiv - Cancer Biology 2021Quote: ... H460 shFTH1/pcDNA3 and H460 shFTH1/pcDNA3FTH1 cells were washed twice with cold PBS and incubated for 20 min with 300 μL of 1X Ripa Buffer (Cell Signaling) additioned with HaltTM Protease Inhibitor Single-Use Cocktail ...
-
bioRxiv - Molecular Biology 2021Quote: ... The samples were diluted with lysis buffer without inhibitors and incubated overnight at 4°C with anti-PCNA antibody (1:500, #2586, Cell signaling) under mild agitation ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were briefly washed twice with ice-cold PBS supplemented with 1 mM activated sodium orthovanadate and then lysed using commercially available lysis buffer (Cell Signaling) supplemented with benzonase (ThermoFisher) ...
-
bioRxiv - Molecular Biology 2022Quote: ... washed three times with TBST buffer and incubated over night with either primary antibody (rabbit ant-HA, #3724 and anti-GAPDH, #97166 antibody, Cell Signaling). The following day ...
-
bioRxiv - Immunology 2022Quote: Cells were cultured in 8 well chamber slides and fixed in 4% paraformaldehyde and permeabilized using 0.1% saponin in blocking buffer (HBSS containing 3% BSA, 0.2% gelatin, and 0.02% NaN3) and stained with Rabbit anti-mouse YAP1 (Cell Signaling Technology, D8H1X), FITC-conjugated phalloidin ...
-
bioRxiv - Immunology 2021Quote: The primary CD4+ T cells were lysed in the RIPA buffer with protease/phosphatase inhibitor cocktail (Cell Signaling Technology, Danvers, MA). After determining the protein concentration for each cell lysate ...
-
bioRxiv - Immunology 2021Quote: ... Δagr and in-vitro-transcribed RNAIII (3 µg ml-1, 1 µg ml-1) were lysed in 30 µl of 1X cell lysis buffer (Cell Signaling) supplemented with 1X protease cocktail inhibitor (Roche ...
-
bioRxiv - Molecular Biology 2019Quote: ... The transblot sheets were blocked with 5% normal donkey serum in TBST buffer Tris-buffered saline (10 mM Tris-HCl pH 7.5, 75 mM NaCl, #9997S, Cell Signaling Technology) and 0.1 % Tween 20] and incubated with diluted primary antibody for 1 hr at room temperature or overnight at 4°C ...
-
bioRxiv - Immunology 2019Quote: ... Membranes were blocked for 1 h at RT in Odessy blocking buffer (Licor) and incubated overnight at 4°C with the following antibodies: IRF3 (Cell Signaling) 1:1000 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Western blots were then blocked with LI-COR blocking buffer and incubated overnight with primary antibodies anti-STAT3 (Cell Signaling, #4904), anti-pSTAT3 (Cell Signaling ...
-
bioRxiv - Molecular Biology 2019Quote: ... protein sample was transferred into a new microcentrifuge tube and the final volume was adjusted to 100 μL with general cell lysis buffer (Cell Signaling). Reduction solution (50 μL ...
-
bioRxiv - Neuroscience 2020Quote: ... Blocking buffer was manually removed and replaced with either anti-Asymmetric Di-Methyl Arginine antibody (1:500, Cell Signaling Technology 13522) or anti-Symmetric Di-Methyl Arginine antibody (1:800 Cell Signaling Technology 13222 ...