Labshake search
Citations for Cell Signaling Technology :
451 - 500 of 681 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... anti-phospho-s6 ribosomal protein (pS6 Ser240/244) (rabbit monoclonal, catalog #2215S, Cell Signaling Technology, MA, RRID: AB_331682). Secondary Alexa Fluor 594 (goat anti-rabbit ...
-
bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were the following: anti-NOTCH1 targeting C terminus of the protein (Cell signaling, catalog no. 3608); anti-NOTCH2 targeting C terminus of the protein (Cell signaling ...
-
bioRxiv - Microbiology 2020Quote: ... rabbit anti S6 ribosomal protein (5G10; 1:103 dilution in 5% BSA, Cell Signaling Technology, Leiden, The Netherlands). Secondary Abs. ...
-
bioRxiv - Immunology 2020Quote: ... the 96 well plate was coated with human ACE2 recombinant protein (1μg/ml, mFc tag; Cell Signaling Technology). The plate was blocked with PBS containing 5% skim milk for 2 h at RT ...
-
bioRxiv - Cell Biology 2021Quote: ... Antibodies against the following proteins were used: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (clone 14C10) (#2118, Cell Signaling, USA), E-cadherin (clone HECD-1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... DNA and proteins were eluted from beads by adding 150 μL of 1X elution buffer (#7009, Cell Signaling) and heating at 65°C for 30 min ...
-
bioRxiv - Immunology 2022Quote: ... Protein detection was carried out using anti-human LC3-I/II antibody (Cell Signaling Technology, Danvers, MA, #12741).
-
bioRxiv - Immunology 2022Quote: ... Proteins were detected using human monoclonal primary antibodies anti-cleaved caspase-3 (1:250, clone 5A1E, Cell Signaling) and anti-β-actin (1:1,000 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples of equal protein concentration were prepared in reducing and denaturing conditions (Cat. No. 7722, Cell Signaling Technology).
-
bioRxiv - Physiology 2022Quote: ... blocked in 5 % BSA TBST for 1 h and reprobed with S6 ribosomal protein antibody (#2317, Cell Signaling) using the same protocol as above ...
-
bioRxiv - Physiology 2022Quote: ... 0.1 % Tween-20) for 1 h and incubated overnight with phospho-S6 ribosomal protein antibody (#2211, Cell Signaling) in 1 % BSA TBST at 4 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... and total proteins were extracted from the cells by adding Cell Lysis Buffer (Cell Signaling Technology, MA, USA) containing 500 mM NaCl ...
-
bioRxiv - Neuroscience 2022Quote: ... rabbit anti-phospho ribosomal protein S6 (Ser235/236; Cell Signaling Technology, catalog no. 4858, 1:1000 for WB), mouse anti-ribosomal protein S6 (Cell Signaling Technology ...
-
bioRxiv - Bioengineering 2023Quote: ... Protein quantification was performed on single 1 mm gel biopsy punches lysed in buffer (#9803S, Cell Signaling Technologies) containing Benzonase Nuclease (#E8263 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein was run on an SDS PAGE and probed with anti-his antibody (Cat # 2365, Cell Signaling Technologies).
-
bioRxiv - Microbiology 2023Quote: ... Expression of iKd PfGEXP15 protein was checked by Western blotting using anti-HA (1/1000, Cell signaling C29F4) followed by anti-Rabbit IgG (1/20000 ...
-
bioRxiv - Cancer Biology 2023Quote: ... each lysate was pre-cleared with 20 μL of pre-washed Protein A agarose beads (Cell Signaling 9863S). Next ...
-
bioRxiv - Molecular Biology 2023Quote: ... along with 10 μl of pre-washed Protein-A Agarose beads (Cat. No. 9863S, Cell Signaling, Danvers, MA). The tubes were left on a rotating disc at 40C overnight for pre-clearing the lysates ...
-
bioRxiv - Cancer Biology 2021Quote: Recombinant mouse Ifng and human IFNG proteins (with carrier) were purchased from Cell Signaling Technology (catalog #39127 and 8901). They were reconstituted with sterile water at a concentration of 0.1 mg/ml ...
-
bioRxiv - Genetics 2019Quote: ... as described previously.21 The following antibodies were used for western blotting: anti-S6 ribosomal protein (Cell Signaling Technology), anti-phospho-S6 ribosomal protein (Ser240/244 ...
-
bioRxiv - Immunology 2019Quote: ... The following primary antibodies were used to probe protein levels by immunoblotting: anti-GAPDH (Cell Signaling Technology, clone D16H11), anti-β-Actin (Santa Cruz Biotechnology ...
-
bioRxiv - Immunology 2020Quote: ... protein from transfected 293T cells was analyzed by western blotting with an anti-GFP antibody (clone D5.1, Cell Signaling). This corresponded with an increase in protein size from 27 to 32 kDa by western blotting (Fig S2C) ...
-
bioRxiv - Immunology 2019Quote: ... 40 μg of sheared chromatin/sample was incubated with protein G magnetic beads with α-RelB (10544, Cell Signaling) or α-NF-κB2 antibodies (37359 Cell Signaling ...
-
bioRxiv - Physiology 2019Quote: ... and equal amounts of protein were incubated overnight at 4 °C with rabbit acetyl-lysine antibody (AcK; Cell Signaling). Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology) ...
-
bioRxiv - Biochemistry 2021Quote: Fibroblasts and podocytes were washed twice with PBS and proteins were extracted with the lysis buffer (Cell Signaling Technology) which protease and phosphatase inhibitors (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto nitrocellulose and incubated with primary antibody: LC3 (rabbit polyclonal, Cell Signaling, cat # 12741S, 1:1000), SQSTM1/p62 (guinea pig polyclonal ...
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to PVDF transfer membranes and incubated with primary antibodies against pAKT serine 470 (Cell Signaling Technology) (1:1000 ...
-
bioRxiv - Cancer Biology 2022Quote: ... S6-ribosomal protein (pS235/S236) (D57.2.2E) and SYK (pY525/Y526) (C87C1) were from Cell Signaling Technologies (Leiden, The Netherlands). Antibodies against Btk (pY223)/Itk (pY180 ...
-
bioRxiv - Physiology 2022Quote: ... Protein lysates were incubated overnight at 4 °C with rabbit acetyl-lysine (Ac-K, #9441) from Cell Signaling Technology. Protein densitometry was measured using Image J software (National Institutes of Health ...
-
bioRxiv - Immunology 2022Quote: ... and detection of indicated proteins was carried out using the following antibodies: Aiolos (clone D1C1E, Cell Signaling, 1:20,000), Bcl-6 (clone K112 ...
-
bioRxiv - Plant Biology 2019Quote: ... Phosphorylated (activated) MAPK proteins were detected using anti-phospho-p44/42 MAPK T202/Y204 (pERK) antibodies (Cell Signaling; 9101). SlM3Kα proteins were detected using of anti-HA antibodies (Krackeler ...
-
bioRxiv - Genetics 2020Quote: ... and Myc-tagged Rrp46 and EXOSC5 proteins were detected with anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling). As loading controls ...
-
bioRxiv - Plant Biology 2021Quote: ... The proteins were separated by SDS-PAGE and detected by anti-phospho-p44/42 MAPK antibodies (Cell Signaling #4370) and anti-actin antibodies respectively.
-
bioRxiv - Cancer Biology 2019Quote: ... Protein expression was analyzed by immunoblotting for expression of phospho-EGFR (Y1068, 1:1000, Cell Signaling, Danvers, MA, USA), total EGFR (1:1000 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Antibody against PDZ Binding Kinase (PBK)/T-LAK cell-originated protein kinase (TOPK) was purchased from Cell Signaling Technology, Inc ...
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were detected with horseradish peroxidase (HRP)-linked anti-mouse IgG and anti-rabbit IgG secondary antibodies (Cell Signaling) and SuperSignal West Dura Extended Duration Substrate (Thermo Scientific) ...
-
bioRxiv - Neuroscience 2021Quote: ... and anti-phosphorylated ribosomal protein S6 (p-rpS6240/244, 1:1500, rabbit polyclonal, Cell Signaling Technology Cat# 2215, RRID:AB_331682). Sections were then washed with PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... INSR protein complexes were eluted from the beads by incubation in Blue Loading Buffer (50 mM DTT, Cell Signaling) at 95°C for 5 min.
-
bioRxiv - Cell Biology 2021Quote: ... INSR protein complexes were eluted from the beads by incubation in Blue Loading Buffer (50 mM DTT, Cell Signaling) at 95°C for 5 min and were analyzed by standard western blot procedures ...
-
bioRxiv - Microbiology 2022Quote: ... For protein detection the following primary antibodies were used at the indicated dilutions: GAPDH (Cell Signaling, #2118, 1:4000), γH2AX (Cell Signaling ...
-
bioRxiv - Genetics 2022Quote: ... Protein lysates were incubated overnight at 4°C with mouse α-Myc antibody (1:400, [Cell Signaling Technologies, #2276]) on a rotisserie tube rotator ...
-
bioRxiv - Cell Biology 2022Quote: ... MAFA and MAFB protein levels were normalized to endogenous β-actin by immunoblotting with anti-MAFA (Cell Signaling 79737), anti-MAFB (Cell Signaling 41019S ...
-
bioRxiv - Cancer Biology 2022Quote: Protein samples were collected and lysed using a radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysates were quantified by Bradford assay (Bio-Rad) ...
-
bioRxiv - Plant Biology 2022Quote: ... AtRsmD-4×Myc fusion proteins were detected using a mouse anti-Myc monoclonal antibody (1:10,000; Cell Signaling Technology).
-
bioRxiv - Neuroscience 2022Quote: ... The lysate was centrifuged at 12 000 g for 15 min and the supernatant with 0.25 mg total protein was incubated with 0.5 μg anti-Ire1α (Cell Signaling) antibody for 4 hours at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... The levels of HuR protein were detected using western blot and anti-HuR antibody (Cell Signaling Technology, USA, 12582S). The probes used are listed in the Supplementary Table 6.
-
bioRxiv - Microbiology 2023Quote: ... The extent of protein knockdown was determined by immunoblotting with an anti-c-Met antibody (#8198, Cell Signaling Technology), anti-E-cadherin antibody (#3195 ...
-
bioRxiv - Microbiology 2022Quote: ... For protein detection the following primary antibodies were used at the indicated dilutions: AKT (Cell Signaling, #9272, 1:1000), phospho-AKT T308 (Cell Signaling ...
-
bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred to supported nitrocellulose membrane and probed with p44/42 MAPK antibody from Cell Signaling Technology (CST) (9102 ...
-
bioRxiv - Developmental Biology 2023Quote: ... 2 μg of protein were added to pre-washed 50 μL streptavidin Sepharose bead conjugates (Cell Signaling Technology, 3419S) and rocked overnight at 4°C ...