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Citations for Cell Signaling Technology :
201 - 250 of 658 citations for NGS ChIP Seq kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Ubiquityl-Histone H2B (K120) (#5546, for WB, IF, and ChIP) and FOXO1 (#2880, for WB and IHC) from Cell Signaling Technology; SUZ12 (ab175187 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2021) using 25μg of chromatin and 10ug of either anti-HIF1α ChIP antibody (Novus) or anti-rabbit IgG (Cell Signaling Technologies).Chromatin was immunoprecipitated overnight at 4C and HIF1α binding sites assayed via RT-qPCR using 2μl of either input ...
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bioRxiv - Cancer Biology 2023Quote: ... The DNA fragments were purified using the DNA purification buffers and spin columns (ChIP, CUT&RUN, Cell Signaling Technology, cat# 14209) and SYBR Green-based real-time PCR was conducted ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μl cell lysates served as a source of the E3 ligase CHIP (cells were lysed in Cell lysis buffer (9803, Cell Signaling Technology). After reactions ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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bioRxiv - Immunology 2022Quote: ChIP assays for histone modifications were performed as described previously (Aoyama et al., 2018) using an anti-H2AK119ub1 (#8240S; Cell Signaling Technology) and an anti-H3K27me3 (#07-449 ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting mixtures of protein-DNA and antibody were further incubated with ChIP-Grade Protein G Magnetic Beads (9006, Cell Signaling Technology). After sequential washing with low- and high-salt solutions ...
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bioRxiv - Neuroscience 2024Quote: ... sheared chromatin was centrifuged at 16,000 x g for 20 minutes at 4 °C and dissolved in 1x ChIP buffer (Cell Signaling Technology, #57976). 2% input was taken and chromatin was incubated with antibodies overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Normal dermal fibroblasts were treated with TGFβ (10 ng/ml, Cell Signaling) and/or JQ1 (1 µM ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-Akt (Cell Signaling Technology: 9272, Lot#: 5, 17 ng/ml), rabbit anti-phospho-Akt (pS473 ...
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bioRxiv - Cancer Biology 2021Quote: ChIPs were performed using cross-linked chromatin from Capan-2 cells and either anti-BCL6 antibodies (1;1000, Cell Signaling Technology Cat# 14895, RRID:AB_2798638), anti-STAT1 antibodies (1;1000 ...
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bioRxiv - Immunology 2021Quote: ... Equal amounts of chromatin were used per IP step with exceptional performance (XP®) monoclonal antibodies validated for ChIP application (Cell Signaling Technology). Rabbit monoclonal antibodies were added in particular dilution based on an optimized concentration evaluated across a wide variety of commercial monoclonal antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Histone modification ChIPs were performed by using 10 µg of chromatin each incubated with the recommended amount of H3K27Ac (Cell Signaling #8173, 5ul), H3K4me1 (Cell Signaling #5326 ...
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bioRxiv - Genomics 2023Quote: Native ChIP was performed using the protocol described previously [29] on homogenized liver cells using anti-CENP-A (Cell Signaling Technologies, Cat # C51A7), H3K9me3 (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... RAW264.7 cells were always pretreated with IFN-γ (15 ng/ml, Cell Signaling) for 48 hrs before each assay ...
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bioRxiv - Cell Biology 2021Quote: ... A 0-to-200 ng/mL platelet-derived growth factor (PDGF, Cell Signaling) gradient in DMEM was established for migration of human fibroblasts ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... Following blocking for 1h with 10% normal goat serum (NGS: Cell Signaling, Inc.), the primary antibody (Table 2) ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ng/mL human β Nerve Growth Factor (Cell Signaling Technology, Cat# 5221SC), and 3 μg/mL + 7 μg/mL FRD+U (5-Fluoro-2′-deoxyuridine ...
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bioRxiv - Neuroscience 2024Quote: ... 10 ng/mL Human β nerve growth factor - (Cell Signaling Technology, Cat# 5221SC)
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bioRxiv - Genetics 2019Quote: ... Antibodies used in ChIP are: rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (Cell Signaling 13448S, Lot 1; 5 µL per IP); mouse monoclonal anti-pan-N-terminal-Lamin A/C antibody E1 (Santa Cruz Biotechnology sc-376248 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were harvested and IP was performed on the sonicated chromatin material using a ChIP grade antibody -anti-SP1 antibody (Cell Signaling Technologies, Clone D4C3) or IgG (negative control) ...
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bioRxiv - Biophysics 2019Quote: ... cells were treated with recombinant human TGF-β1 (10 ng/ml, Cell Signaling Technology) 59 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-VASP (clone 9A2, Cell Signaling Technology: 3132, Lot#: 5, 21 ng/ml), rabbit anti-Akt (Cell Signaling Technology ...
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bioRxiv - Systems Biology 2023Quote: ... and 0% FBS media with 10 ng/mL human IL1β (Cell Signaling Technology, 8900SC), or TGFβ1 and IL1β combined ...
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bioRxiv - Immunology 2024Quote: ... Anti-AhR and anti-5-loxygenase (5-LOX) antibodies (Ab) for immunoblot assay and anti-trimethyl H3K4 (H3K4me3) Ab for chromatin immunoprecipitation (ChIP) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-ALOX5AP (FLAP ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 10 ng/ml TGF-β1 (Cell Signaling Technology, Danvers, MA, USA) unless otherwise described ...
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bioRxiv - Cell Biology 2022Quote: ... and another where cells were treated with 100 ng/mL nocodazole (Cell Signaling Technology, 2190S) for 18 hours at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 ng/ml TGF-β1 (Cell Signaling Technology, Danvers, MA, United States, Cat#8915). All cell lines were tested regularly for Mycoplasma infection ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS and blocked simultaneously in 2% normal goat serum (NGS – Cell Signaling Technology, 5425) in PBS for one hour with agitation ...
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bioRxiv - Systems Biology 2024Quote: ... U-2 OS cells underwent treatment with 50 ng/mL recombinant IFN-γ (Cell Signaling). The cytokine was diluted in 0.5% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were incubated with kinase buffer containing 200 ng of human RB protein (Cell Signaling, 9303) and 200 µM ATP for 30 min at 37°C with gentle rocking ...
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bioRxiv - Cancer Biology 2020Quote: ... with or without the addition of the cytokines TNF-alpha (20 ng/mL, Cell Signaling Technology) and TGF-beta 1 (10 ng/mL ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-phospho-Akt (pS473, clone D9E, Cell Signaling Technology: 4060, Lot#: 25, 48 ng/ml), mouse anti-GAPDH (ProteinTech ...
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bioRxiv - Cancer Biology 2022Quote: ... Spheroids were then washed with DPBS and blocked with 5% Normal Goat Serum (NGS) (Cell Signaling Technology) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Nonfat dry milk (cat. #9999) and normal goat serum (NGS; cat. #5425) were purchased from Cell Signaling Technology. ReverTra Ace qPCR RT master mix with a gDNA Remover kit was purchased from TOYOBO ...
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bioRxiv - Physiology 2023Quote: ... Nonfat dry milk (cat. #9999) and normal goat serum (NGS; cat. #5425) were purchased from Cell Signaling Technology. 2-(3-(but-3-yn-1-yl)-3H-diazirin-3-yl)ethan-1-amine and 1H-benzotriazol-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate (BOP ...
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bioRxiv - Cell Biology 2021Quote: EMT was induced in the HMLE cell line by chronic stimulation with 4 ng/ml TGFβ1 (Cell Signaling Technology) for 14 days9 ...
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bioRxiv - Cell Biology 2021Quote: EMT was induced in the MCF10A cell line by chronic stimulation with 5 ng/ml TGFβ1 (Cell Signaling Technology) for eight days22 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with a rabbit anti-FLAG M2 antibody (142 ng/mL) (Cell Signaling Technology, Danvers, MA, USA) in 1% BSA–PBS for 1 h at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were next incubated with rabbit active caspase-3 primary antibody (0.768 ng/mL, Cell Signaling Technology, Leiden, The Netherlands), followed by incubation in goat anti-mouse Texas Red secondary antibody (20 μg/mL in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were added in 1% NGS in PBST overnight at 4°C (anti-CDK9, clone C12F7, Cell Signaling Technology, monoclonal IgG antibody generated in rabbit ...
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bioRxiv - Developmental Biology 2024Quote: ... which consists of Basal Human TS Cell Medium supplemented with 100 ng/mL of neuregulin 1 (NRG1, 5218SC, Cell Signaling), 7.5 μM A83-01 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples containing 10 µg of DNA were immunoprecipitated with 375 ng (10 µl) of TCF7L2 antibody (Cat. no. 2569, Cell Signaling). Incubation with protein G agarose beads and elution of precipitated chromatin was conducted according to ChIP-IT High Sensitivity kit protocol (Cat ...
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bioRxiv - Microbiology 2022Quote: ... we stimulated NFκB-dependent gene expression by treating cells with purified human TNF (100 ng per ml; Cell Signaling Technology, Inc.) in 1X Opti-MEM reduced serum media (Gibco Life Sciences ...
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bioRxiv - Immunology 2023Quote: ... 0,05% Tween-20 and 1% NGS in PBS) with diluted primary antibodies: (LSD1, 1:200, Cell Signaling Technology, Cat. No. 2184S), (MUC2 ...
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bioRxiv - Neuroscience 2024Quote: Free-floating sections were washed twice in 1x PBS for 10 min and then incubated with 10% normal goat serum (NGS, #5425, Cell Signaling) blocking solution (1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Free-floating sections were washed twice in 1x PBS for 10 min and then incubated with 10% normal goat serum (NGS, #5425, Cell Signaling) blocking solution (1x PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then washed with CyTOF permeabilization buffer and the barcoded samples were combined before being incubated with antibodies in a solution containing 10% NGS (Cell Signaling #5425). Cells were then washed with CyTOF staining buffer and fixed with 10% formalin (Merck #F5554 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then rinsed three times with PBS before adding 100 ng/mL DAPI and fluorescent secondary antibody (Cell Signaling Technology 4413S) diluted 1:500 in PBS containing 1% BSA and 0.3% Triton-X for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... and immunoprecipitated overnight at 4 °C with the pre-prepared antibody-beads complex, which contains 700 ng H3K4me3 antibody (15410003, Digagenode) or H2Aub antibody (8240, Cell Signaling Technology) and 5 μl Dynabeads Protein A/Protein G ...