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Citations for Cell Signaling Technology :
51 - 100 of 734 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... total myc-GluA1/2 was labeled with rabbit anti-myc antibody (71D10, Cell Signaling Technology) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... and co-labeled with β-tubulin III (β3-Tub; 1:2000, 5568S; Cell Signaling Technologies) to visualize axons within the lesion ...
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bioRxiv - Immunology 2023Quote: ... T/NK lymphocyte were labeled using a rabbit anti-ZAP70 monoclonal antibody (99F2 – Cell Signaling) diluted at 1:600 in Pierce™ Immunostain Enhancer solution (Thermofisher ...
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bioRxiv - Physiology 2019Quote: ... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
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bioRxiv - Immunology 2022Quote: ... T cells were labeled with an anti-mouse CD3ε Ab (Cell Signaling technology, Danvers, MA, USA). Tissue sections were observed using the light microscopy mode of an inverted fluorescence phase-contrast microscope (BZ-9000 ...
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bioRxiv - Cancer Biology 2022Quote: ... TMT10 labeled Peptides with acetylated K were enriched with Acetyl-Lysine Motif kit (Cell Signaling Technology) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies were then labeled with goat-anti-rabbit IgG conjugated to horseradish peroxidase (Cell Signaling). Blocking solution was 5wt% non-fat dry milk in TBST (Tris Buffered Saline with Tween® 20).
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bioRxiv - Developmental Biology 2021Quote: ... Animals were labeled with 1:800 anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies), BODIPY FL-Phallacidin ...
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bioRxiv - Microbiology 2020Quote: ... Alexa Fluor® 647-labeled rabbit anti-β-tubulin (9F3, Cell Signaling Technology and EPR16775, Abcam) and Alexa Fluor® 594-labeled mouse anti-ZO1 (1A12 ...
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bioRxiv - Cancer Biology 2023Quote: ... The OC cells were labeled with Pan-Keratin (1:200, Cell Signaling Technology, Cat. No. 4545S) and CAFs with αSMA (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... Animals were labeled with 1:400 anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies), BODIPY FL-Phallacidin ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were then labeled with goat-anti-rabbit IgG conjugated to horseradish peroxidase (Cell Signaling). Blocking solution was 5wt% non-fat dry milk in TBST ...
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bioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA protein assay kit from Cell Signaling Technology. Thirty micrograms of total protein were mixed in a reducing sample buffer and used for mitochondrial apoptosis assay per the kit instruction ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the following primary antibodies overnight: rabbit anti-RAD18 NB100-61063 (Novus) (1:200) and mouse anti-biotin (D5A7) (1:200) (Cell signaling). The rest of the protocol follows Proximity Ligation Assay as above.
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase labeled donkey anti rabbit or donkey anti mouse antibodies were from Cell Signaling (Beverly, MA). Recombinant mouse SPARC protein (cat ...
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bioRxiv - Molecular Biology 2021Quote: ... and BrdU-labeled cells were detected using goat anti-Mouse Fab′2 Alexa Fluor 488 (Cell Signaling). The cells were counterstained with PI ...
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bioRxiv - Neuroscience 2023Quote: ... The CaMKII⍺ was labeled with anti-CaMKII-α (6G9) (Cell Signaling Technology, #50049, RRID: AB_2721906, 1:250) and goat anti-rabbit 647 secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... protein lysis buffer (Cell Signaling) containing a protease inhibitor cocktail was added and the mixture homogenized ...
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bioRxiv - Genetics 2019Quote: ... The culture medium was then replaced with medium containing 50 mM biotin and cultured for a further 24 hours when the cells were lysed using RIPA Buffer (Cell Signaling Technology) with 1 µM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Developmental Biology 2021Quote: ... and apoptotic cells were labeled using rabbit anti-cleaved caspase-3 antibody (catalog # 9661, Cell signaling, 1:100). To avoid non-specific binding of the antibodies ...
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bioRxiv - Immunology 2022Quote: ... Alexa Fluor 647 (Ax647)- or cyanin 5 (Cy5)-labeled goat- or anti-rabbit antibodies purchased from Cell Signaling.
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were labeled with either anti-PU.1-PE (clone 9G7, 1:50, Cell Signaling Technology, Danvers, MA) or IgG-PE isotype control (clone DA1E ...
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bioRxiv - Systems Biology 2023Quote: ... We labeled active and total β-catenin and β-actin control bands using primary antibodies (Cell Signaling Technologies, Danvers ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa 647–labeled anti–phospho-Fos antibody (1:100; catalog no. 8677, Cell Signaling Technology. RRID: AB_11178518) for 30 min at 4°C followed by one wash in 0.8 ml of PBS ...
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bioRxiv - Biochemistry 2021Quote: ... and followed with detection by Neutravidin-DyLight-800 (Pierce 22853) for biotin-ADP ribosylated Ub and anti-Ub-H2A (Cell signaling Technology #8240) for H2A ubiquitylation.
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bioRxiv - Cell Biology 2023Quote: ... we used either HRP-conjugated protein A or HRP-conjugated protein G (both from Cell Signaling) because of high background from the immunoglobulin bands when we used secondary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... S6 Ribosomal protein (5G10, Cell Signaling), BIM (C34C5 ...
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bioRxiv - Immunology 2019Quote: ... Protein A agarose beads (Cell signaling) were used for IgG pulldown ...
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bioRxiv - Physiology 2022Quote: ... and Biotinylated Protein ladder (Cell Signaling) were included on the gels ...
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bioRxiv - Molecular Biology 2022Quote: ... protein lysis buffer (Cell Signaling Technology) containing a protease inhibitor cocktail was added ...
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bioRxiv - Cell Biology 2023Quote: ... one for each protein (Cell Signaling). Each membrane piece was then incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... fixed cells were labeled with a one-step staining solution containing anti-RelA (1:400, Cell Signaling Technology, #8242), Nano-secondary AF568 (Chromotek ...
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bioRxiv - Physiology 2023Quote: ... blots were washed with TBS-T three times and then incubated in TBS-T containing 5% non-fat dry milk and anti-biotin horseradish peroxidase (Cell Signaling, Danvers, MA, USA) and HRP-conjugated donkey anti-chicken antibody (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... and the endoplasmic reticulum (ER) was labeled with a rabbit anti-Calnexin antibody (1:1000, Cell Signaling Technology, CA, USA). The Golgi apparatus was labeled with a rabbit anti-GM130 antibody (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... and horseradish peroxidase (HRP)-labeled anti-mouse IgG (H + L) (cat #, 7076S, 1: 2,000, Cell Signaling Technology, Danvers, MA, USA) were used as primary and secondary antibodies ...
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bioRxiv - Immunology 2019Quote: ... Quantified protein (between 200-500 µg) was incubated with protein-specific or isotype control antibody (Cell Signaling Technology) in lysis buffer at 4°C overnight with head over tail rotation ...
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bioRxiv - Immunology 2020Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins bound to Protein-A Dynabeads were pulled down using a magnetic separation rack (Cell Signaling, no. 14654). After 5 washes in NP-40 lysis buffer ...
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bioRxiv - Immunology 2023Quote: The protein concentration was adjusted to 30 μg by adding protein lysis buffer (Cell Signaling Technology, Danvers, MA) and 4x loading buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... Western blotting was performed as described above and biotinylated (internalized) β1-integrin was detected by immunoblotting with horseradish peroxidase (HRP)-conjugated anti-biotin antibody (Cell Signaling Technology, 7075, 1:15000) and imaging with the chemiluminescence film-based system ...
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bioRxiv - Molecular Biology 2021Quote: Protein A magnetic beads (Cell Signaling Technology) were pre-washed with cell lysis buffer twice ...
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bioRxiv - Developmental Biology 2021Quote: ... containing a Protein A slurry (Cell Signaling). A non-specific antiserum (control IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... S6 ribosomal protein (2217; Cell Signaling Technology), Akt1 (2938 ...
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bioRxiv - Immunology 2021Quote: ... coupled to Protein G beads (Cell Signaling) overnight with rotation at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... containing a Protein A slurry (Cell Signaling). A non-specific antiserum (control IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... protein A/G agarose beads (Cell Signaling) were added and allowed to rotate for another 2h ...
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bioRxiv - Neuroscience 2021Quote: ... Fixed fish were immunofluorescently labeled pERK (Phospho-pp44/42 MAP Kinase) and tERK (pp44/42 MAP Kinase) antibodies (Cell Signaling Technologies) and Alexa fluorophore conjugated secondary antibodies at 1:500 dilutions ...
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bioRxiv - Genetics 2019Quote: ... Antibodies used in immunofluorescence are: Alexa 647-conjugated rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (labeled at Cell Signaling, product ID 97262BC ...
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bioRxiv - Genetics 2019Quote: ... Cells were blocked with Blocking buffer (FACS buffer supplemented with 5% normal goat serum) and incubated with Alexa-647-conjugated rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (labeled at Cell Signaling, product ID 97262BC ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then washed three times with PBS and total cells were labeled with a 1:1,000 dilution of DRAQ5 (Cell Signaling Technology) for 5 min at 4°C ...