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Citations for Cell Signaling Technology :
151 - 200 of 10000+ citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... PanAKT was immunoprecipitated from the lysate using the rabbit anti-mouse Akt antibody (C67E7, Cell Signaling Technologies 4691) and the PierceTM Classic Magnetic IP/Co-IP kit (ThermoFisher 88804) ...
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bioRxiv - Cell Biology 2020Quote: Protein lysates were prepared using 1X RIPA analysis (10X RIPA buffer, #9806, Cell Signaling Technologies, Danvers, MA, USA) containing protease and phosphatase inhibitors (#5872S ...
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bioRxiv - Genomics 2019Quote: Protein lysates were obtained using ice-cold 1X RIPA buffer containing protease and phosphatase inhibitors (Cell Signaling 9806), and freshly supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Microbiology 2019Quote: ... Remaining lysate (∼1 mL) was mixed with pre-washed Myc-Tag Magnetic Bead Conjugate #5698 (Cell Signaling Technology) or Glutathione Magnetic Agarose Beads #78601 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The lysate samples were denatured by mixing with Blue Loading Gel Dye and DTT (Cell Signaling Technology, 7722). Primary antibodies used in this experiment were as follows ...
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bioRxiv - Cell Biology 2023Quote: ... The rest of the lysate was split in half and either 1:100 Oct4 antibody (Cell Signaling, 2750) or rabbit IgG isotype control (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... each lysate was pre-cleared with 20 μL of pre-washed Protein A agarose beads (Cell Signaling 9863S). Next ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... Lysates were probed using anti-β-actin and anti-GAPDH (1:1000 dilution, Cell Signaling Technology, # 4967, # 97166). Either anti-rabbit-680 or anti-mouse-800 (1:5000 dilution ...
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bioRxiv - Cell Biology 2022Quote: Sorted MuSCs were cultured overnight in 96 well plates and cAMP measurements were performed on cell lysates using the Cyclic AMP XP® Assay Kit (cat Cyclic AMP XP® Assay Kit (cat # 4339, Cell Signaling Technology) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysates (20 µg) were subjected to SDS-PAGE and immunoblotted with specific antibodies against pFRS2a (#3864; Cell signaling Technology, Beverly, MA, USA), FRS2 (sc-17841 ...
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bioRxiv - Genomics 2020Quote: ... was added to sonicated lysate along with 0.48 μg of c-Jun antibody (cat. no. 9165; Cell Signaling Technology) or equal amount of rabbit IgG (cat ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μg of nuclear protein lysates were incubated with 2 μg of either Myc-Tag antibody (Cell Signaling Technology) or an endogenous NUP210-specific antibody (Bethyl Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: ... Lysate was incubated for 4 h at 4°C with anti-ECSIT2 antibody39 or IgG control (Cell Signaling, #2729), followed by overnight precipitation with 30 µL of washed Protein A sepharose 4B beads (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: ... Protein lysates were incubated overnight at 4 °C with rabbit acetyl-lysine (Ac-K, #9441) from Cell Signaling Technology. Protein densitometry was measured using Image J software (National Institutes of Health ...
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bioRxiv - Pathology 2021Quote: Tissue lysates were generated using 1 mL per 100 mg tissue in lysis buffer (Cell signaling Technologies, Danvers, MA). Total protein contents were quantified using a Pierce BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Physiology 2019Quote: ... lysates were ran out on 4-12% gradient gels and probed with anti-phospho(T389) p70S6K (Cell Signaling 9205) and anti-p70S6K (Cell Signaling 2708 ...
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bioRxiv - Cancer Biology 2022Quote: ... the lysates were incubated at 4°C for 5 h with antibodies for BRD4 (Cell Signaling Technology; 1:100). Antibody/antigen complexes were recovered using Dynabeads Protein A/G (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... Protein lysates were incubated overnight at 4°C with mouse α-Myc antibody (1:400, [Cell Signaling Technologies, #2276]) on a rotisserie tube rotator ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... the sonicated lysate was then separated to a new tube and primary antibodies was added (anti-H2AK119Ub Cell Signaling D27C ...
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bioRxiv - Immunology 2023Quote: ... and the remaining lysate was incubated with Phospho-AMPK Substrate Motif mAb mix (Cell Signaling Technologies; catalog No. 5759) for 12 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were then incubated overnight (12 h) at 4 °C with mouse anti–NCS-1 (1:500; Cell Signaling). Samples were subsequently incubated for 2 h with Protein-G–Sepharose (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were incubated with 10 ug of anti-Nup98 antibody or an irrelevant isotype control (IgG DA1E, Cell signaling) overnight and subsequently incubated with protein G-beads for 2h ...
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bioRxiv - Cancer Biology 2019Quote: ... lysates were immunoblotted with use of an anti-Brn2 antibody (Santa Cruz Biotechnology, sc-393324 or Cell Signaling Technology, #12137), anti-p63 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... and lysates were then incubated overnight with primary anti-LRRC23 or Rabbit IgG (#2729, Cell Signaling Technology, Danvers, Massachusetts, USA) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal protein concentration were incubated overnight in 4 °C with an anti-Src antibody (Cell Signaling Technology, #2108). Complexes were pulled out using protein-A sepharose beads (GE-Healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: ... Up to 30 µg of protein lysate was separated by SDS-PAGE and transferred to nitrocellulose membranes before antibody incubation (IκBα: Cell Signaling, CS9242 ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were sacrificed 6 days after noise exposure and whole cochlear lysates were prepared in lysis buffer (9803; Cell Signaling) after adding protease (cOmplete ULTRA Tablets 05892791001 ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... The bead-immobilized GTP-bound RhoA and total RhoA in the lysates were probed by immunoblotting with anti-RhoA antibody (Cell Signaling).
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bioRxiv - Biochemistry 2020Quote: IP was performed on hepatocytes lysates using antibodies against LxRxx[S*/T*] and Rxx[S*/T*] phospho-motifs (both Cell Signaling) with Pierce Protein A/G Magnetic Beads according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Microbiology 2020Quote: ... 250 μL of lysate were incubated with 2 μL of mouse Myc-Tag antibody clone 9B11 (Cell Signaling Technology, Cat. 2276S) for 4 h with rotation at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates were co-imunoprecipitated with anti-HA beads (HA-Tag C29F4 Rabbit mAb Sepharose® Bead Conjugate Cell Signaling #3956 RRID:AB_10695091) overnight at 4 °C under gentle agitation ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mg of lysate representing one biological replicate was incubated for 2 hours at 4 °C with anti-Kv11.1 antibody (Cell Signaling #12889) covalently coupled to protein A/G magnetic beads (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... 5mg of lysate was incubated with 30ul of MyOne Streptavidin C1 Dynabeads or 5mg of Myc-Tag (9B11) Mouse mAb (Cell Signaling, #2276) antibody for 3 hours with subsequent 1-hour incubation with 30ul of Protein G Dynabeads for streptavidin pulldown and myc tag IP ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were first titrated on CD34+ fetal liver lysates and subsequently used at the following dilutions: Anti-GATA1 (Cell Signaling, 1:5), anti-STAG2 (Cell Signaling ...
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bioRxiv - Genomics 2022Quote: ... acid-extracted histone lysate was prepared accordingly (Shechter et al., 2007) and subjected to western blotting using anti-H3K9ac (Cell Signaling, 9649), H3K14ac (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... the same amount of each cellular lysate was analyzed by gel electrophoresis and western blot using anti-WDR5 antibody (D9E1I; Cell Signaling #13105), anti-HSP90 antibody (F-8 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were immunoprecipitated from precleared lysates overnight at 4°C using 4 μg of control IgG (rabbit or mouse, Cell Signaling Technology) or indicated antibody ...
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MIIP downregulation promotes colorectal cancer progression via inducing adjacent adipocytes browningbioRxiv - Cancer Biology 2023Quote: ... Protein lysates containing 1 mg total protein were precleaned and precipitated with indicated antibody or IgG (Cell Signaling Technology, Danvers, MA, US) for 6 h at 4°C ...
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bioRxiv - Pathology 2023Quote: Western blot analysis was performed using whole liver lysates for HNF4α (Perseus Proteomics, PP-H1415-00) and cMyc (Cell Signaling Technology, 56055), as described previously [7] ...
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bioRxiv - Genomics 2024Quote: ... Cell lysate was centrifuged at 21,000 x g for 10 minutes and supernatant was mixed with Blue Loading Buffer (Cell Signaling Technology #7722) per the manufacturer’s recommended ratio ...
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bioRxiv - Developmental Biology 2024Quote: MCF10A cells from 2D collagen stimulation assays were pelleted by centrifugation and lysed using 1X RIPA Buffer containing both 1x protease inhibitor cocktail and 1x phosphatase inhibitor Lysate was precleared with Protein-A Magnetic Beads (73778, Cell Signaling Technology).
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bioRxiv - Cancer Biology 2020Quote: ... 90% of the pre-cleared lysates were subsequently added to prewashed glutathione agarose beads from Active Ras Detection kit (Cell signaling Technology, #8821) for 1 hour at 4 °C under constant rocking ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated internalized proteins in lysates were captured by NeutrAvidin beads (Pierce) and eluted by incubating in Blue Loading Buffer (50 mM DTT, Cell Signaling, Danvers, MA) containing 50 mM DTT at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: Senescence-associated beta-galactosidase in HSVSMC lysates was quantified using the Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) (Cell signaling Technology). Measurement was done on cell lysates with the same total protein concentration ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole protein lysates were analyzed for total protein expression and lysine-methylated peptides were enriched using reagents from Cell Signaling Technology (catalogue no. 14809). In short ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control antibody (Cell Signaling Technology, 1:300), or anti-IRF1 antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... 300 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control (Cell Signaling Technology, 1:300), anti-Ollas (Novus ...
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bioRxiv - Microbiology 2020Quote: Cells were lysed in cell lysis buffer (Cell Signaling) containing protease inhibitor cocktail (Sigma-Aldrich ...