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Citations for Cell Signaling Technology :
451 - 500 of 813 citations for CEACAM 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The blotted membranes were blocked with 5% nonfat dry milk (Cell Signaling Technology; #9999S) in Tris-buffered saline containing Tween-20 (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies used include: total eIF2a (Cell Signaling Technology #9722, 1:1000, in 5% BSA), phosphor-Ser51-eIF2a (Cell Signaling Technology #9721 ...
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bioRxiv - Biochemistry 2022Quote: ... and cullin-RING E3 ubiquitin ligases with 5 µM MLN4924 (Cell Signaling Technology, 85923) for 5 h prior to cell harvest.
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bioRxiv - Physiology 2021Quote: ... Rabbit polyclonal antibodies to Fyn (Cell Signaling Technology, 4023S, 1:2500 in 5% BSA); Rabbit polyclonal antibodies to Phospho-Src Family (Tyr416 ...
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bioRxiv - Molecular Biology 2021Quote: ... + 0.5 μL 1M DTT + 5 μL 200X PIC (SimpleChIP Kit #9003, Cell Signaling Technology) and incubated on ice 10 min with mixing every 3 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were co-treated with 5 μM U0126 (Cell Signaling Technology, Danvers, MA, USA) or 20 μM perifosine (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... phospho-GSK3β (Ser9) (1:1000 in 5% BSA and TBST, Cell Signaling Technology, #93360), and GAPDH (1:5000 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-VASP (clone 9A2, Cell Signaling Technology: 3132, Lot#: 5, 21 ng/ml), rabbit anti-Akt (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2021Quote: ... Other antibodies used were anti-phospho-Smad1/5 (pMad) (rabbit 1:100, Cell Signaling), anti-DIAP1 (mouse ...
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bioRxiv - Genetics 2019Quote: ... then stained for pMad (anti-phospho-Smad 1/5, 41D10, 1:200, Cell Signaling), and Flag (anti-Flag ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were blocked and permeabilized in PBS containing 5% normal goat serum (Cell Signaling) and 0.4% Triton-Rx100 (Alfa Aesar ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked 1h with 5% of non-Fat dry milk (NFDM, Cell signaling) diluted in TBS-T buffer (10mM Tris-HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... The Rabbit monoclonal to phospho-Smad 1/5 (Cell Signaling Technology Cat# 9516, RRID:AB_491015). Mouse monoclonal to BMPR1A (Thermo Fisher Scientific Cat# MA5-17036 ...
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bioRxiv - Cancer Biology 2023Quote: ... Pre-cleared chromatin was immunoprecipitated with 5 µL of H3K27me3 antibody (Cell Signaling 9733) overnight at 4°C with constant rotation ...
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bioRxiv - Immunology 2023Quote: ... whole cell lysates were pre-cleared with 5 μg anti-IgG (Cell Signaling Technology) or 5 μg anti-Ikzf1 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated with 5 mL of 1x RBC lysis buffer (Cell signaling, 46232S) for 5 minutes on ice to remove red blood cells ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μg of the p-STAT3 antibody (catalog# 9131, Cell Signaling, Danvers, Massachusetts, USA) was introduced in the ChIP procedure using protein G agarose beads ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-Akt (pan, C67E7, Rabbit, #4691, Cell Signaling, 1:1000 in 5%BSA/TBST), monoclonal anti-α-Tubulin (mouse ...
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bioRxiv - Genetics 2022Quote: ... human anticentromere (1:100; Antibodies Inc., cat# 15-234) and mouse monoclonal anti-H3K9me2/3 (1:100, Cell Signaling, cat# 5327) primary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: Human tissue samples and cell preparations were harvested in Complete RIPA Buffer - 1× radio immunoprecipitation buffer (10× RIPA; 9806, Cell Signaling) and one tablet each of PhoStop and Complete Mini (04906845001 and 04693124001 ...
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bioRxiv - Cell Biology 2021Quote: Human paraffin embedded pancreas biopsies were sectioned to 3 μm and immunostained with antibodies against MAFB (Cell Signaling Technologies, cat#41019), ARX (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: Protein expression levels of PANX1 were examined by western blotting using a rabbit monoclonal antibody against human PANX1 (D9M1C, Cell Signaling). Actin was also detected as a control by western blotting using a mouse monoclonal anti-β-actin antibody (Santa Cruz) ...
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bioRxiv - Cancer Biology 2022Quote: Cells with stable expression of FLAG-tagged human PDE6D were lysed in 1X cell lysis buffer (#9803, Cell Signaling, Danvers, MA) and incubated with anti-FLAG M2 Affinity Gel (A2220 ...
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bioRxiv - Bioengineering 2019Quote: ... cardiac fibroblasts were pre-treated in plastic tissue-culture flasks with 10 nM human TGF-β1 (Cell Signaling Technology, cat# 8915LC) for 48h ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360 ...
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bioRxiv - Cancer Biology 2020Quote: ... Human-specific EpCam-647 (clone VU1D9, #5447) and rabbit anti-human γH2AX antibodies (Phospho-Histone H2A.X Ser139; clone 20E3, #9718) from Cell Signaling Technologies, and EpCam-APC antibody (clone EBA-1 ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Human CDH5 and ICAM-1 protein levels were evaluated by SDS PAGE using rabbit anti-CDH5 (Cell signaling technology Cat# 2500) and mouse anti-ICAM-1 (Santa Cruz biotechnology ...
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bioRxiv - Microbiology 2022Quote: ... we stimulated NFκB-dependent gene expression by treating cells with purified human TNF (100 ng per ml; Cell Signaling Technology, Inc.) in 1X Opti-MEM reduced serum media (Gibco Life Sciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with rabbit-anti-human ABCB1 (E1Y7B; mAb #13342; Cell signaling Technology; dilution 1:1000) and mouse-anti-human β-actin (sc-47778 ...
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bioRxiv - Bioengineering 2020Quote: ... Sections were then separately incubated with anti-p-Smad1/5 (1:500, Cell Signaling Technologies), anti-Yap (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... Jurkat cells (107) were suspended in 5 mL enriched DMEM and SB203580 (Cell Signaling Technology) was added from 10 mM stock to a final concentration of 10 μM ...
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bioRxiv - Biochemistry 2022Quote: ... H3K36me2 (10 × 106 cells, 1 μg anti-H3K36me2, Cell Signaling Technologies, Cat#2901, Lot#5). Dynabeads Protein G (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Sections (5 μm thick) were stained with anti-E-Cadherin (Cell Signaling, Cat No 3195), anti-Snail (Santa Cruz ...
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bioRxiv - Physiology 2021Quote: ... Rabbit polyclonal antibodies to CD36 (D8L9T) (Cell Signaling Technology, 14347S, 1:1000 in 5% BSA); Rabbit polyclonal antibodies to Fyn (Cell Signaling Technology ...
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bioRxiv - Physiology 2021Quote: ... Rabbit polyclonal antibodies to MIF (E7T1W) (Cell Signaling Technology, 87501, 1:1000 in 5% BSA); Rabbit polyclonal antibodies to CD36 (D8L9T ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubation was performed in 5% dry milk with anti-rabbit (Cell Signaling, 7074S) or anti-mouse (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-FU-D113-#4) using an anti-H3K27me3 antibody (Cell Signaling Technology, Ref: 9733-C36B11). Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... HRP-linked antibody (1:1000 dilution in 5% BSA in TBST buffer, Cell signaling #7074) was incubated at 4 °C for 1h ...
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bioRxiv - Neuroscience 2021Quote: ... gels were transferred onto nitrocellulose membranes and blocked in 5% nonfat milk (Cell Signaling Technology) in TBS-Tween 20 (TBST) ...
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bioRxiv - Genomics 2022Quote: ... brought to 100 μL with ultrapure water and 5 μL PMSF (200 mM, Cell Signaling) were added and incubated for 10 minutes at rt to block Proteinase K activity ...
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bioRxiv - Genomics 2023Quote: ... The pre-cleared chromatin was then incubated with 5 ul of H3K9ac (Cell signaling #9649), 2 ul of H3K23ac (AbCam #ab177275) ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... minced and incubated with 5 ml 1x Red Blood Cell Lysis Buffer (Cell Signaling Technology) for 5 min with manual shaking ...
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bioRxiv - Physiology 2024Quote: ... followed by HRP-conjugated secondary antibodies (TST-T in 5% milk; 1:5000; Cell Signaling) for 1 h ...
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bioRxiv - Cancer Biology 2020Quote: ... and rabbit monoclonal vinculin antibody (clone E1E9V; #13901, 1:1000; epitope: amino terminus of human vinculin protein) were purchased from Cell Signaling Technology.
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bioRxiv - Cancer Biology 2020Quote: ... Anti-Phospho-p44/42 MAPK (ERK1/2—Thr202/Tyr204, CAT# 9101) and anti-MCL-1 (D35A5 rabbit anti-human primary, CAT# 5453) antibodies were purchased from Cell Signaling Technology. Anti-ELP4 (rabbit anti-human primary ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-human myosin light chain 2 (D18E2) [with cross-reactivity to mouse] (catalog # 8505, RRID:AB_2728760; Cell Signaling Technology, Danvers, MA, USA), and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718 ...
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bioRxiv - Molecular Biology 2020Quote: ... the rabbit monoclonal anti-Actin antibody (12748S, rabbit monoclonal antibody to a synthetic peptide corresponding to residues near the carboxy terminus of human β-actin protein, Cell Signaling) and the rabbit monoclonal anti-acetyl-histone H3 (Lys9 ...
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bioRxiv - Genomics 2020Quote: To assess editing efficiency,1 × 106 cells were taken from culture five days post electroporation and stained with the following primary anti-human antibodies: Cbl-b (Clone D3C12, Cell Signaling Technologies) followed by AlexaFluor 647 F(ab’)2 goat anti rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... The following antibodies have been used on human PDAC tissue of figure 1b or mouse PDAC tissue from figure 2b: pan-Keratin (#4279, Cell Signaling Technology), p-ERK (#9101 ...