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Citations for Cell Signaling Technology :
551 - 600 of 638 citations for Borrelia burgdorferi sensu stricto ZS7 Flagellin Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Proteins were immunoprecipitated from precleared lysates overnight at 4°C using 4 μg of control IgG (rabbit or mouse, Cell Signaling Technology) or indicated antibody ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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bioRxiv - Immunology 2020Quote: ... To detect phosphorylated ERM proteins a rabbit anti-phospho–ezrin (Thr567)/radixin (Thr564)/moesin (Thr558) (pERM) antibody was used (Cell Signaling Technology). Isotype control staining was performed using rabbit IgG isotype mAb Alexa Fluor 647 (DA1E ...
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bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... The spike protein and fragments were detected by rabbit monoclonal antibodies (mAb) against the 6-His tag (12698S) from Cell Signaling Technology. The nitrocellulose (NC ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then prepared for Western blot analysis and total protein was incubated with E-Cadherin (1:1000, Cell Signaling Technology, #3195), Fibronectin (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated proteins were detected by probing the membranes with an anti-phospho-c-Met antibody (Tyr1234/1235, #3077, Cell Signaling Technology) or an anti-phospho-EGFR antibody (Tyr1068 ...
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bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Bis(sulfosuccinimidyl)suberate disodium salt (BS3; Dojindo, #B574) was used to crosslink the antibodies to protein G magnetic beads (Cell Signaling Technology). Benzonase (Merck ...
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bioRxiv - Bioengineering 2023Quote: ... Western blot analysis was performed to assess total and phosphorylated p70S6K1 (70kDa ribosomal protein S6 kinase) (T389, Cell Signaling Technology; Boston, MA) as well as 4EBP1 (4E-binding protein 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... HER2 protein detection on EVs was performed by staining samples with HER2/ErbB2 (29D8) – PE-conjugated antibody or Isotype Control (Cell Signaling Technology) and CellMask plasma membrane stains (C10046 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein lysates were separated by SDS-PAGE and proteins of interests detected with the following antibodies: phosphorylated (p-VASP) Ser239 (rabbit, 1:1000, Cell Signaling #3114S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Biophysics 2023Quote: ... blocked with 5% bovine serum albumin for 1 hour at room temperature and incubated overnight with a primary antibody against the principal ventricular GJ protein Cx43 phosphorylated at Ser368 (pCx43, 1:1000, #3511S, Cell Signaling Technologies), at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Every gel included two molecular-weight markers for separate regions of interest (High Range Color-Coded Prestained Protein Marker; Cell Signaling Technology; and Novex Prestained Protein Standard ...
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bioRxiv - Neuroscience 2023Quote: ... anti-Phospho-p70 ribosomal protein S6 kinase (Thr389) (108D2) (pS6K) (rabbit monoclonal, #9234, RRID: AB_2269803, Cell Signaling Technology; 1:1000 for WB), anti-p70 S6K α (S6K)(mouse monoclonal clone H-9 ...
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bioRxiv - Immunology 2023Quote: ... cycloheximide (CHX) final concentration of 8 ug/mL was used to arrest protein translation and Actinomycin D (Cell Signaling Technology, Cat. 15021) was used to arrest transcription by treating cells with 10 ug/mL Actinomycin D where indicated ...
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bioRxiv - Cancer Biology 2023Quote: ... or EV fraction was loaded alongside a biotinylated ladder and/or pre-stained protein molecular weight markers (Catalog numbers 7727 and 59329, respectively, Cell Signaling Technology) on NuPAGE™ Bis-Tris™ pre-cast gels (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Specimens were incubated overnight at 4 °C with rabbit anti-SARS-CoV-2 nucleocapsid protein (Cell Signaling Technology, USA, #26369, 1:200), mouse anti-ACE2 (Cell Signaling Technology ...
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bioRxiv - Microbiology 2024Quote: ... the ADP-ribosylated proteins were visualized using chemiluminescence using the SignalFire ECL Reagent or the SignalFire Elite ECL Reagent (Cell Signaling Technology) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h at room temperature and blotted with an antibody against phosphorylated upstream frameshift protein 1 (phospho-UPF1) (pUPF1; 1:1000, Cell Signaling Technologies) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: MCF10A cells from 2D collagen stimulation assays were pelleted by centrifugation and lysed using 1X RIPA Buffer containing both 1x protease inhibitor cocktail and 1x phosphatase inhibitor Lysate was precleared with Protein-A Magnetic Beads (73778, Cell Signaling Technology).
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bioRxiv - Cell Biology 2024Quote: ... cFos protein was labelled in the same manner using rabbit polyclonal antibody c-Fos (9F6) (1: 1000, Cell Signaling Technology Inc, 2250) and Alexa Fluor 488 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... and then the supernatant fraction was incubated with anti-BCR-ABL antibody overnight at 4°C and subsequently with protein A magnetic beads (73778, Cell Signaling Technology) for another 4 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... For visualization of SARS-CoV-2 infected cells via detection of N protein in Calu-3 cells we used anti SARS-CoV-1/2 Nucleocapsid Mouse anti-N clone (Cell Signaling, 1C7C7) 1:1000 50uL/well.
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bioRxiv - Cancer Biology 2021Quote: ... or of 35 mg (for mouse tumors) total proteins using the PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit (Cell Signaling Technology), according to manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Phosphorylated and total proteins were identified by immunoblotting using the following primary antibodies: Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 and α-Tubulin (DM1A) Mouse mAb #3873 (Cell Signaling Technologies) at 1:1000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Antibody labelling was performed with mouse anti-SARS-CoV-2 S protein (Abcalis, clone AB68-A09, #ABK68-A09-M) and secondary antibody anti-mouse Alexa488 (Cell Signaling Technology, #4408), each step followed by three washing steps with PBS containing 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins of interest were detected with HRP-conjugated horse anti-mouse IgG antibody (1:5000, 7076S, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA) or HRP-conjugated goat anti-rabbit IgG antibody (1:5000 ...
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bioRxiv - Pathology 2022Quote: Cardiac proteins were extracted using freeze crusher (SK-100, Tokken Inc., Chiba, Japan) and cell lysis buffer (#9803, Cell Signaling Technology, MA, USA). Protein lysate was separated by SDS-PAGE and transferred onto polyvinylidene fluoride membranes ...
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bioRxiv - Biophysics 2021Quote: The phosphorylation of proteins was quantified by western blotting using antibodies against pEGFR (#4407 for pY1173 and #3777 for pY1068; Cell Signaling Technology (CST)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Aliquots of the cell lysates containing 50 μg of protein were submitted to SDS-PAGE and immunoblotted for TP53 (Cell Signaling Technology, #32532) and β-actin (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed on 6% acrylamide SDS-PAGE gels followed by immunoblotting with rabbit anti-myc (1:3000, 71D10, Cell Signaling Technology, 2278S). In vitro dephosphorylation experiments were performed in three biological replicates.
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bioRxiv - Neuroscience 2019Quote: ... Formalin fixed brains were paraffin embedded and analyzed using the following antibodies according to immunohistochemistry protocols described previously (Chakrabarty, 2010; Chakrabarty, 2015): Glial Fibrillary Acidic Protein (GFAP, Cell Signaling, 1:1000) and ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cancer Biology 2019Quote: ... 15 μg of extracted proteins were separated by SDS-PAGE and revealed by antibodies directed against actin (1: 1000, Cell Signaling Technology #8457), GAPDH (1 ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein of interest was detected using an appropriate antibody specific for phospho-EGFR (Tyr1068) (2234; Cell Signaling Technology [CST], Danvers, MA, USA), EGFR (2232 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit monoclonal anti-SorLA antibody specific to the N-terminus of human SorLA protein (1:1,000; D8D4G #79322; Cell Signaling, Danvers, MA, USA) for the detection of SorLA759 proteins ...
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bioRxiv - Microbiology 2021Quote: ... was used for western blotting at a dilution of 1:1,000 and rabbit polyclonal antiserum reactive against protein kinase A (PKA) substrates (Cell Signaling Technology, Danvers, MA) was used for western blotting at a dilution of 1:1,000 ...
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bioRxiv - Biochemistry 2022Quote: ... phosphorylated at Ser293 PDHA and acetylated proteins were estimated by western-blotting using primary antibodies from Cell Signaling Technology (Danvers, MA, United States) with the product numbers #5490 ...
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bioRxiv - Immunology 2022Quote: ... Cas9 expression was validated by flow cytometry using intracellular staining of cas9 protein with anti-cas9 monoclonal antibody (7A9-3A3, Cell Signaling, cat #48796). Cas9 activity was assessed by transducing the cas9-BV2 cells with pXPR_047 ...
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bioRxiv - Genomics 2023Quote: ... Correct expression of the mAID-Dam-Lamin B1 fusion protein as well as the wildtype Lamin B1 protein was confirmed using Immunofluorescence staining using an anti-HA antibody at 1 in 500 dilution (Cell Signaling Technologies, C29F4) and an anti-LaminB1 antibody at 1 in 500 dilution (Abcam ...
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bioRxiv - Genetics 2022Quote: ... We quantified changes in protein levels relative to control using Image Lab software after normalization to β-actin or GAPDH (Cell Signaling Technologies), as indicated ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Each blot was then washed thrice in 1X TBST for 5 minutes and proteins were detected using either LumiGLO/Peroxide Reagent or SignalFire™ ECL reagent (7003 and 6883, respectively, Cell Signaling Technology) and an iBright™ imaging system (ThermoFisher) ...
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bioRxiv - Biophysics 2023Quote: ... mAb #3288) and Lysosome Associated Membrane Protein 1 (LAMP1) specific rabbit monoclonal antibody (cat. no. mAb #9091) were purchased from Cell Signaling Technology (USA). Anti-caveolin-1 rabbit polyclonal antibody was acquired from BD Biosciences (USA ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to all native and/or phosphorylated proteins (see Supplementary Table S1 for details) except for WT1 were purchased from Cell Signaling Technology (Boston, MA). The C-terminal anti-WT1 antibody (C-19 ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling Technologies, Cat. No 9863), for 1 hour in 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling Technologies, Cat. No 9863), for 1 hour in 4°C ...
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bioRxiv - Microbiology 2020Quote: ... of the sample was incubated with 3 μg of antibody (or 3 μg of IgG as control) and 30 μl of protein G magnetic beads (Cell Signaling Technology, Cat. 7008) at 4 °C overnight ...