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Citations for Cell Signaling Technology :
401 - 440 of 440 citations for BIgG SAP Goat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies used were horse anti-mouse HRP or goat anti-rabbit HRP which were both diluted 1:1,000 in block (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Microbiology 2022Quote: ... The fluorescent-labelled Alexa Fluor 647 (AF647) goat anti-Rabbit IgG monoclonal antibody was from Cell Signaling Technologies (MA, USA; Cat. n° 4414).
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bioRxiv - Immunology 2022Quote: ... the membrane was incubated for one hour at RT with peroxidase-conjugated goat anti-rabbit secondary antibody (Cell Signaling Technology, #7074, 1:1000) and washed again ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies against phospho-STAT1 and STAT1 and horseradish peroxidase-conjugated goat anti-rabbit antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with incubate for 1 hour at room temperature in 1:500 goat anti-rabbit Alexa Fluor 555 (Cell Signaling Technology, 44135), 1:400 Alexa Fluor 488 Phalloidin (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed with Bond Wash solution 2x 10s and blocked with a phosphate-buffered saline (PBS) solution containing 5% normal goat serum (5425S, Cell Signaling, Danvers, MA) and 0.5% TritonX-100 (T8787 ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated at room temperature with HRP-conjugated secondary antibodies ((goat anti-rabbit HRP conjugated, Cell Signaling, Cat #: 7074, 1:5,000 dilution) (goat anti-mouse HRP conjugated ...
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bioRxiv - Immunology 2023Quote: ... and intracellular location of 4H2 or IgG control was detected by immunostaining with an Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Cell Signaling, Danvers, MA) as previously described (32) ...
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bioRxiv - Developmental Biology 2023Quote: ... MoBU-1, mouse, 1:250, ThermoFisher #B35128; secondary antibody: anti-mouse IgG Alexa Fluor® 555 Conjugate, 1:500, goat, Cell Signaling #4409). First ...
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bioRxiv - Genetics 2022Quote: ... Neurons were subsequently blocked with 5% normal goat serum in 1x PBS for 1 h and then incubated with primary antibodies as follows: anti-GFP (Cell signaling, #2555S, R), anti-Gephyrin (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were blocked with TBST/5% Normal Goat Serum for 1 hour at RT before overnight incubation with primary antibody diluted in Diluent CST (Cell Signaling Technology, 8112) at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Membranes were incubated for 1 h at room temperature with appropriate secondary antibodies: goat anti-rabbit (1:2000, Cell Signaling Technology, Cat. 7074), goat anti-rat (1:10000 ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were washed and then blocked using 1% Goat serum in TBS for one hour at room temperature before adding primary antibodies to detect Cd45 (Cell Signaling, 1:500) and Cd11b (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... permeabilized cells were blocked for 30 minutes at room temperature (RT) in 5% (v/v) normal goat serum (NGS) (Cell Signaling Technology, #5425) DPBS solution ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were detected using goat anti-mouse or anti-rabbit IgG (H+L) Alexa fluor 488 or 647 (Cell signaling Technologies/ Bioconcept) secondary antibodies diluted 1:500 in 1% BSA 0.1% Triton-X 100 in PBS for 3 hours in the dark at room temperature ...
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bioRxiv - Genetics 2019Quote: ... Secondary antibodies (a donkey anti-goat IgG-HRP antibody, Santa Cruz Biotechnology, sc-2020, and a horse anti-mouse IgG-HRP antibody, Cell Signaling Technology, #7076), conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... was used at dilution 1:20 and incubated with the cell solution at 4°C for 30 min before staining with AlexaFlour 488 goat anti-mouse (Cell Signaling, 1:100 dilution) for another 30 min at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... sc-2030, Santa Cruz; goat anti-mouse IgG-HRP, sc-2005, Santa Cruz; Mouse Anti-rabbit IgG-HRP [L27A9] mAb, #5127, Cell Signaling Technology).
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then washed 3 times with TBST and the secondary goat anti-rabbit IgG horseradish peroxidase (HRP)-conjugated antibody (1:5000; cat. no. 7074, Cell Signaling Technology, The Netherlands) was added and allowed to bind at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, catalog # 7074) or goat anti-mouse IgG (1:3,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, Catalog # 7074) or goat anti-mouse IgG (1:3000 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed three times with 0.5% BSA–PBS and incubated with a horseradish peroxidase-conjugated goat anti-rabbit antibody (30 ng/ml) (Cell Signaling Technology, Danvers, MA, USA) in 1% BSA–PBS for 1 h at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, catalog # 7074). Then the membranes were washed with TBST four times ...
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bioRxiv - Cell Biology 2023Quote: ... Appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies were used as follows: goat anti-rabbit IgG HRP linked Antibody (Cell Signaling Technology, 7074S, 1:2000), or goat anti-mouse IgG HRP (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed with PBS-T and they were incubated with goat anti-rabbit IgG H&L Alexa Fluor 488 (Cell Signaling Technology, MA, USA) for 90 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... anti-Actin and HRP- labeled goat-anti-rabbit or anti-mouse IgGs for Western blot assay were purchased from Cell Signaling Technology (Danvers, MA). Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Biochemistry 2020Quote: ... Free-floating sections were blocked with 5% donkey serum for 1.5 hours before overnight incubation at 4°C with primary antibodies (goat anti-Albumin antibody, Abcam ab19194, 1:100 dilution; rabbit anti-V5 antibody, Cell Signaling Technology, 1:500 dilution). Sections were washed ...
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bioRxiv - Neuroscience 2020Quote: ... incubated in HRP-conjugated secondary antibodies (donkey anti mouse HRP, Jackson ImmunoResearch, Cat. #715-035-150 or Goat anti rabbit HRP: Cell Signaling Technology, Cat. # 7074S). Membranes probed for p62 were washed with 0.01% TBS-Triton X and incubated in biotinylated anti-guinea pig (goat polyclonal ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were labeled with the following primary antibodies: SOX2 (goat, R&D, AF2018, 3 μg/mL) and NeuN (rabbit, Cell Signaling Technology, 12943S, 1:500) diluted in antibody blocking solution (1X PBS pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was washed four times with PBS-T and incubated with DyLight 800 goat anti-rabbit IgG (Cell Signaling Technology, cat. no. 5151, RRID:AB_10697505) diluted 1:10,000 in PBS-T containing 5% (w/v ...
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bioRxiv - Bioengineering 2019Quote: ... 1:50, Sigma-Aldrich, cat. nr. HPA050007; goat αV5 antibody, 1:350, Abcam cat. nr. ab95038; and, rabbit αHA, 1:800, Cell Signaling cat. nr. C29F4) at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... and secondary horseradish peroxidase (HRP)-conjugated antibodies goat anti-rabbit IgG (#7074) and horse anti-mouse IgG (#7076) were purchased from Cell Signaling Technology (Danvers, MA, USA).
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was quenched by 3% hydrogen peroxidase prior to 4-hour incubation at 4°C with 5% normal goat serum (Cell Signaling Technology®, Danvers, MA) and rabbit anti-DSG1 (clone EPR677766(B) ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were then incubated with the following secondary antibody: Alexa Fluor 594 goat anti-mouse (1/1000, Cell Signaling Technology Cat# 8890, RRID: AB_2714182) at room temperature for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... Proteins transferred to a PVDF membrane were detected with 1:1,000 of a rabbit α-HAdV-C2 ADP78-93 antibody (Tollefson et al., 2003) followed by goat α-rabbit-HRP (7074, Cell Signaling Technology, Danvers, USA). Protein bands were visualized using ECL Prime Western Blotting Detection Reagent (GE Health Care ...
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bioRxiv - Cancer Biology 2019Quote: ... blocked in 8% goat serum and incubated overnight at 4°C with anti-phospho Axl (R&D, AF2228, diluted 1:200) CK11 (Cell signaling, CST 4545, diluted 1:200), followed by fluorescently labelled secondary antibodies goat anti mouse 488 (Abcam ab98637) ...
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bioRxiv - Microbiology 2020Quote: ... 1:1,000 R3 rabbit α-pVII/VII (Ulf Petters-son of Uppsala University) and visualized using a goat α-rabbit-HRP (7074, Cell Signaling Technology, Danvers, USA) and ECL Prime Western Blotting Detection Reagent (GE Health Care ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed for 30 min with TBS-0.05% Tween 20 (TBS-T) and primary antibodies were detected using horseradish peroxidase-conjugated goat anti-mouse secondary antibody diluted 1:2000 (Cell Signaling Technology, Inc., Danvers, MA, USA) and incubated at room temperature for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... washed three times for 10 min in PBS and incubated for 1 h in blocking solution (5% normal goat serum, Cell Signaling Technology #5425, 0,3% Triton X-100 in PBS) at room temperature ...