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Citations for Cell Signaling Technology :
601 - 650 of 653 citations for Alkaline Phosphatase Colorimetric Activity Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: The chromatin immunoprecipitation assay was performed as previously reported using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, Cat No. 9003)47 ...
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bioRxiv - Immunology 2022Quote: ... Fly heads were cut off and placed into lysis buffer from PTMScanHS Ubiquitin/SUMO Remnant Motif Kit Protocol (#59322, Cell Signaling, UK), that contained 5% SDS in TEAB buffer (T7408 ...
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bioRxiv - Developmental Biology 2023Quote: ... Detection of cell death due to exposure to ChABC was achieved using Annexin V-FITC Early Apoptosis Detection Kit (Cell Signaling, USA), done according to the kit user guide with minor modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hr at room temperature followed by development using chromogenic substrate DAB (SignalStain® DAB Substrate Kit, Cell Signaling, Cat# 8059s) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Enrichment for ubiquitinated peptides was conducted using a PTM Scan® Ubiquitin Remnant Motif [K-ε-GG] kit (Cell Signaling, Danvers, MA) following the vendor’s recommended procedures ...
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bioRxiv - Bioengineering 2023Quote: A reduced glutathione assay was developed for in-chip measurements by adapting the Cellular Glutathione Detection Assay Kit (Cell Signaling Technology #13859). The monochlorobimane reagent was prepared by reconstitution in DMSO per manufacturer directions ...
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bioRxiv - Cancer Biology 2023Quote: Cleaved PARP in cell lysates was measured using PathScan® Cleaved PARP (Asp214) Sandwich ELISA Kit (Cell Signaling Technology, Danvers, MA, USA) as per manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: The 1 mg TMT-labeled peptide fraction underwent phosphotyrosine peptide enrichment using the PTMscan HS Phospho-Tyrosine (P-Tyr-1000) Kit (Cell Signaling Technology) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... blocked and probed with the indicated antibodies (Rb Antibody Sampler Kit #9969) and β-actin from Cell Signaling Technologies (Danvers, Massachusetts, USA).
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bioRxiv - Cancer Biology 2023Quote: ... cellular and nuclear fractions from HNSCC cell lines and tissues were prepared using the cell fractionation kit purchased from Cell Signaling (#9038) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were rinsed with pre-warmed 1x PBS and fixed with 1x fixative solution provided by senescence beta-galactosidase staining kit (9860, Cell Signaling Technology) for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: 200,000 primary NSCs either stimulated with IL1α (10ng/ml) or left unstimulated were used for CUT&RUN assay using the CUT&RUN kit from Cell Signaling Technologies® #86652 according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... freshly prepared by trypsin-EDTA digestion were used to perform CUT&RUN with the CUT&RUN Assay Kit (catalog no. 86652, Cell Signaling Technology), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-PSA/KLK3 (D6B1) PARP (9542) and cell cycle regulation sampler kits (9932 and 9870) were obtained from Cell signaling technologies (Danvers, MA). The secondary antibodies (IR dye 680 and IR dye 800 ...
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bioRxiv - Cancer Biology 2021Quote: ... or of 35 mg (for mouse tumors) total proteins using the PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit (Cell Signaling Technology), according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Cells were treated with doxycycline for 7 days and were fix and stained with a Senescence β-Galactosidase Staining Kit (Cell Signaling, 9860) according the manufacture’s instruction and incubated at 37 °C for overnight.
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bioRxiv - Cancer Biology 2019Quote: ... 25 mg tumour tissue / IP were fixed in 1% formaldehyde (Applichem) and processed according to the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology).
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bioRxiv - Cancer Biology 2020Quote: ... Samples were alkylated and digested with trypsin at 37°C overnight and ubiquitinated peptides were enriched with PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology). After Sep Pak desalting ...
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bioRxiv - Cancer Biology 2020Quote: ... 90% of the pre-cleared lysates were subsequently added to prewashed glutathione agarose beads from Active Ras Detection kit (Cell signaling Technology, #8821) for 1 hour at 4 °C under constant rocking ...
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bioRxiv - Neuroscience 2021Quote: SA-β-gal staining was performed according to the protocol of the Senescence-β-Galactosidase Staining Kit (Cell Signaling Technology, Danvers, MA, USA). At least 200 cells were counted in each treatment group from three independent experiments.
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellet was then resuspended in 1 X PBS and cell fractionation was carried out using the Cell Fractionation Kit (Cell Signaling Technology, 9038) according to manufacturer’s protocol.
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bioRxiv - Systems Biology 2021Quote: ... We incubated the cell lysates with Phospho-YAP (Ser397) rabbit antibody and performed a sandwich-ELISA assay by using PathScan Phospho-YAP (Ser397) Sandwich ELISA Kit (Cell Signaling Technology, #57046). We then used a Synergy HTX Multi-Mode Reader (BioTek ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... we analyzed acetylation levels of Histone H3 in HDACI treated cells using the PathScan® Acetylated Histone H3 Sandwich ELISA Kit (Cell Signaling Technology) as described by the manufacturer ...
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bioRxiv - Physiology 2023Quote: ... cells were stained with senescence-associated (SA)-β-galactosidase (gal) according to the manufacturer’s instructions for Senescence β-Galactosidase Staining Kit (Cell signaling, 9860). Cells were counterstained with DAPI ...
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bioRxiv - Cancer Biology 2022Quote: SUM159 and MDA-MB-231 cells were stained for SA-β-gal detection using a Senescence β-galactosidase Staining Kit (#9860, Cell Signaling). Staining was performed by following the manufacturer’s instructions and incubating the stained samples for 48hrs at 37°C without CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-rat secondary antibody was used for detection of primary antibodies and visualized by SignalStain DAB Substrate kit (Cell Signaling Technology, Danvers, MA). All stained tissues were blindly evaluated by pathologists ...
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bioRxiv - Cancer Biology 2023Quote: ... Frozen sections were hydrated with PBS for 10min at RT and stained using Senescence β-Galactosidase staining kit according to manufacturer’s instructions (Cell signaling technology, 9860). The sections were incubated at 37℃ in a dry incubator (no CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Cellular lysates from FLSs were harvested and a ChIP assay was performed using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (9005, Cell Signaling Technology). In brief ...
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bioRxiv - Cancer Biology 2023Quote: Cleaved PARP as an apoptotic marker in cell lysates was measured using PathScan® Cleaved PARP (Asp214) Sandwich ELISA Kit (Cell Signaling Technology) according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated with an appropriate HRP-conjugated secondary antibody (Cell signalling technologies, cat. n. 7074) and LumiGLO® ECL kit (Cell Signaling Technology) was employed following manufacturer’s instruction before to exposure to X-ray film (CytivaTM) ...
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bioRxiv - Systems Biology 2024Quote: The enrichment of lysine acetylated peptides was performed using a PTMScan HS Acetyl-Lysine Motif [Ac-K] Kit (Cell Signaling Technology, Cat #50071) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The rest of the eluate was dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology) as described in the manual ...
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bioRxiv - Genetics 2021Quote: ... and myotube (4 days differentiation in low serum differentiation medium) and ChIP was performed using the Simple ChIP Enzymatic Chromatin IP Kit (Cat# 9003; Cell Signaling Technology; Danvers, MA) following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2020Quote: ... stained with anti-F4/80-PE antibody (eBiosciences #12-4801-80) and isolated using an EasySep™ mouse PE positive selection kit that uses a magnetic bead-based purification strategy (Cell Signaling Technologies #17656) and in the presence of 2mM Icam4/av inhibitor peptide (Xue et al. ...
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bioRxiv - Neuroscience 2021Quote: Approximately 1.2 million microglia were exposed to the indicated treatments followed by cell preparation according to the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003). Samples were cross-linked for 10 min at room temperature with 1% formaldehyde solution ...
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bioRxiv - Cell Biology 2022Quote: ... PathScan® Phospho-Akt2 (Ser474) and Total Akt2 Sandwich ELISA kits were used to quantify AKT2 levels (Cell Signaling Technology #7048 and #7046 respectively) as per manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The PathScan® Phospho-S6Kinase (Thr389) Sandwich ELISA and PathScan® Total S6K Sandwich ELISA Kits were purchased from Cell Signaling Technologies (Danvers, MA). 200,000 FL83B cells/well were seeded onto a 24 well plates and allowed to grow for 48 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Specificity of C12FDG detecting senescent cells was determined in comparison with results obtained with the senescence β-galactosidase staining kit (Cell Signaling Technology #9860) on IMR90 cells that were treated with 100 ng/mL doxorubicin (Sigma ...
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bioRxiv - Genetics 2019Quote: ChIP followed by allele-specific quantitative PCR (ChIP AS-qPCR) [38] were performed in U2OS cell line by using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Catalog# 9003) (Cell Signaling Technology, USA) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: Enrichment of K-ε-GG peptides was performed with the PTMScan ubiquitin remnant motif (K-ε-GG) kit (Cell Signaling Technology, cat. no. 5562) containing anti–K-ε-GG antibody cross-linked to protein A beads ...
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bioRxiv - Cancer Biology 2021Quote: ChIP was performed on 5X106 cells from 2-day control and radiated gliomaspheres using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, #9005S) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed and permeabilized with the Foxp3 transcription factor staining kit (eBioscience, 00-5523-00) prior intracellular staining with the indicated antibodies: H3K9ac (Cell Signaling, 9649, 1:3000), H3K27ac (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for malonylation with anti-malonyl antibody conjugated to agarose beads from the Malonyl-Lysine Motif Kit (Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The following steps were performed according to the manual of the PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling #59322). In brief ...
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bioRxiv - Cell Biology 2023Quote: SA-β-gal staining was performed using a SA-β-gal staining kit according to the manufacturer’s protocol (Cell Signaling Technology, Danvers, MA, USA). In short ...
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bioRxiv - Molecular Biology 2023Quote: ... Apoptotic cells were detected by TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining using in situ apoptosis detection kit (Takata Bio Inc., Shiga, Japan) or immunostaining of cleaved caspase-3 (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Molecular Biology 2023Quote: ... Protein was extracted with RIPA buffer and processed for acetylomic mass spectrometry as described previously55 using PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784).
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bioRxiv - Microbiology 2023Quote: ... peptide samples resuspended in IAP buffer were incubated with 100uL of antibody-bead conjugates specific for acetyl-lysine (Cell Signaling Technologies, PTMScan kits #13416). Immunoprecipitation proceeded overnight at 4°C with gentle mixing ...
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bioRxiv - Cancer Biology 2020Quote: Cells treated for 48 h with NaB were lysed with a lysis solution buffer and acetylation of H3 was measured with PathScan® Acetylated Histone H3 Sandwich enzyme-linked immunosorbent assay (ELISA) Kit (Cell Signaling Technology, Danvers, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were additionally fixed for 10 min in 1% formaldehyde (Applichem, Darmstadt, Germany) and then processed according to the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, Leiden, Netherlands). For chromatin preparation from tissue ...