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Citations for Cell Signaling Technology :
501 - 550 of 594 citations for 6 chloro 5 iodonicotinic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were diluted in PBS-T containing 5% BSA and used 1:1000 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058), 1:1000 for anti-phospho-Akt(Thr308 ...
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bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was then probed with the following primary antibodies diluted in 5 % BSA /PBS-T for overnight at 4 °C: β-actin (1:1000; Cell Signaling Technology), ZO-1 (1.25 μg/ml) ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed three times for 5 min with PBS and incubated in anti-mouse IgG Alexa Fluor® 555 Conjugate (Cell signaling #4409) at a dilution of 1:500 for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked for 1h with 5% BSA in PBS and then stained with rabbit anti-pPLCγ1 antibody (Cell Signaling, #2821, 1/100) overnight at 4C ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Physiology 2019Quote: ... blocked with 5% non-fat powdered milk in TTBS solution and incubated with primary antibodies from Cell Signaling (1:1000; Danvers, MA, USA) against p-IGF1RβY1135 (3918) ...
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bioRxiv - Cell Biology 2019Quote: ... blocked for an hour with 5% BSA-Tris Buffered Saline (TBS) and probed with primary antibodies anti-HA (C29F4) Rabbit monoclonal antibody (mAb) (Cell Signaling Technology®) or mouse raised anti-GFP antibodies (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... Histological sections (5 mm) were stained for immunohistochemistry using a standard protocol with antibodies for LC3A/B (1:200; #12741S; Cell Signaling Technology, USA).
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% skimmed milk and incubated with primary antibodies (rabbit anti-PPAR-γ antibody, Cell Signaling Technology, 1:1000) and HRP-conjugated goat anti-rabbit IgG ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed with Bond Wash solution 2x 10s and blocked with a phosphate-buffered saline (PBS) solution containing 5% normal goat serum (5425S, Cell Signaling, Danvers, MA) and 0.5% TritonX-100 (T8787 ...
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bioRxiv - Biochemistry 2021Quote: ... boiled in sample buffer for 5 min and subjected to immunoblot assay with anti-HA antibody (Cell Signaling Technology, Inc., #3724, Danvers, MA).
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Cells were fixed with 4% paraformaldehyde for 5 min and subsequently permeabilized with 0.2% Triton-X and stained with anti-relB (Cell Signaling Technology, Danvers, USA) followed by goat-anti-rabbit Alexa 546 (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in 5% skim milk in TBST: horse anti-mouse IgG (1:20,000, Cell Signaling Technology, cat# 7076), donkey anti-sheep IgG (1:20,000 ...
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bioRxiv - Bioengineering 2022Quote: ... The membranes were blocked with PBS with 0.1% Tween-20 (PBST) with 5% Blotto for one hour and then incubated with antibodies to phospho-p90RSK (Thr359/Ser363; #9344; Cell Signaling Technology, Inc.) and RSK1/RSK2/RSK3 (32D7 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Primary staining was performed on separate blots with the following antibody dilutions in 5% BSA/TBST: rabbit anti-EZH2 1:1000 (Cell Signaling Technology, 5246S), rabbit anti-CBX2 1:500 (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitates with anti-lamin A/C antibody were analysed in parallel with 5% unbound supernatants by immunoblotting with anti-lamin A/C (Cell Signaling; Cat# #4777) or anti-His (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% BSA in TBS for 1 hr and then incubated overnight at 4° C with anti-pSMAD1/5/8 (1:1000, Cell Signaling Technology, 13820), anti-SMAD1 (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... then probed overnight at 4°C with primary antibodies diluted in a 5% BSA/ 1X TBS-0.1% Tween 20 (TBS-T) solution using either Vinculin (E1E9V) (Cell Signaling, Cat. No. 13901) diluted 1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... For live cell microscopy cells were untreated or treated with 5 μM Aβ fibrils (as described above) pre-incubated with an Aβ antibody (Cell Signaling Technologies, #15126) conjugated to an Alexa Fluor 555 secondary (Invitorogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... and blocked in room temperature with 5% BSA (HiMedia, India) solution for an hour and incubated with respective primary antibodies [FOXM1 (1:2000) (Cell Signaling Technologies, USA); active + pro Caspase-3 (1:3000 ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked in 5% skimmed milk and incubated overnight at 4°C with primary antibodies for UTX (Cell Signaling Technologies #33510), GFP (JL-8 ...
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bioRxiv - Biochemistry 2023Quote: The antibodies used in this study for immunoblotting (Figure 5) were (1) GST-Tag monoclonal antibody 26H1 (Cell Signaling Technologies catalog number 2624S); (2 ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was blocked for 1 h at room temperature with 5 % BSA dissolved in TBS-Tween (0.1 %) and incubated with the primary antibody rabbit anti-phospho-threonine [(Cell Signaling Technology®, #9381) 1:1000] ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were washed three times for 5 min each with TBS-T then incubated in SignalFire ECL Reagent (Cell Signaling Technology, 6883) for 1 min and imaged using a BioRad ChemiDoc imager.
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bioRxiv - Physiology 2023Quote: ... was heated at 90°C for 5 minutes and loaded into acrylamide gels before incubated with primary antibodies against catalase (CAT, #14097, Cell Signaling, 1:5000), 3-nitrotyrosine (3-NT ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated overnight at 4°C with the primary antibody mAb c-MYC (dilution 1:1000 in TBST/5% milk; Cell Signaling Technology, D84C12). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were blocked with TBST/5% Normal Goat Serum for 1 hour at RT before overnight incubation with primary antibody diluted in Diluent CST (Cell Signaling Technology, 8112) at 4°C ...
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bioRxiv - Physiology 2023Quote: ... The PVDF membrane was incubated with 5% w/v non-fat dry milk or 5% BSA for 1 h at room temperature and immunoblotted with anti-HSL (phospho-serine 660) (1:1000, Cell Signaling Technology, 45804S), anti-HSL (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Each blot was then washed thrice in 1X TBST for 5 minutes and proteins were detected using either LumiGLO/Peroxide Reagent or SignalFire™ ECL reagent (7003 and 6883, respectively, Cell Signaling Technology) and an iBright™ imaging system (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... permeabilized cells were blocked for 30 minutes at room temperature (RT) in 5% (v/v) normal goat serum (NGS) (Cell Signaling Technology, #5425) DPBS solution ...
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bioRxiv - Cancer Biology 2024Quote: ... fixed cells were washed twice in PBS with 0.5% BSA prior to incubation with 1:500 dilution of phospho-histone H3 antibody conjugated with Alexa Fluor 488 (Cell Signaling Techonology, USA) for 1 h in the dark at room temperature ...
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bioRxiv - Physiology 2021Quote: ... which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST) and incubated with rabbit anti-pAkt antibody (Cell Signaling Technology, 4060S, 1:1,000 dilution) or rabbit anti-AKT antibody (Cell Signaling Technology 9272S ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2022Quote: ... Pulling down of the pre-cleared chromatin was performed with the use of magnetic beads that were previously incubated with 5 µg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) for 8 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were blocked in 5% bovine serum albumin (BSA) and incubated overnight at 4 °C with primary anti-phospho-SYK (Cell Signaling Technologies, 1:1000) or anti-SYK (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were added after blocking for an hour with 5% non-fat milk: STAT3 and Tyr705 pSTAT3 (Cell Signaling Technology, MA, USA), GAPDH (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were blocked in 5% no-fat dry milk in TBST and immunoblotted with rabbit polyclonal anti-CHD7 1:2000 (Cell Signaling Cat. No.6505S), rabbit polyclonal anti-SOX2 1:2000 (Millipore Cat ...
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bioRxiv - Genetics 2019Quote: ... Antibodies used in ChIP are: rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (Cell Signaling 13448S, Lot 1; 5 µL per IP); mouse monoclonal anti-pan-N-terminal-Lamin A/C antibody E1 (Santa Cruz Biotechnology sc-376248 ...
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bioRxiv - Immunology 2021Quote: Cells were fixed with 0.25% paraformaldehyde in ice-cold PBS for 5 min and incubated with anti-ERp57 (#A484, Rabbit mAb, Cell Signaling Technology, Danvers, MA, USA) 1:100 for 40 minutes ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl PBS-FC containing 5% FBS with 1:100 diluted PE-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA) and incubated for 30 min in the dark ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed with 1X TBST (3 × 5 min) and then incubated with respective secondary antibodies (Horse anti-mice 1:2000; Horse anti-rabbit 1:2000, (Cell signaling technology, MA, USA) and developed by using ECL chemiluminescent reagent (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 5% BSA in TBS-T before incubation with primary antibodies: phospho-AMPKα Thr172 (1:1000, Cell signaling, MA, USA #2535), β-actin (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... After saturation with TBS Tween 5% BSA for 1h and hybridization of the membranes with primary overnight (BCR-ABL Ab3 Calbiochem, pCrkL Tyr207 Cell Signaling, pTYR 4G10 Millipore) and secondary 1h antibodies coupled to HRP ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was blocked for 2-hours in 1% NGS and 0.3% Triton X-100 followed by 2-3 days of 4C° incubation in 1xPBS (5% NGS, 0.3% Triton X-100) with primary antibody(ies): pS6 S235-236 (Cell Signaling, Catalog: 4858, 1:500), pS6 S244-247(ThermoFisher ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked with 5% bovine serum albumin (BSA) in PBS and incubated with anti-APRT antibody or an anti-β- Actin antibody (Cell Signaling Technology, USA) at 4°C overnight followed by the corresponding secondary antibody for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...