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Citations for Cell Signaling Technology :
101 - 150 of 162 citations for 6 Bromo N methylnicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... followed by a secondary goat-anti-mouse HRP antibody (clone NA9310 N; Cell signaling Technology, Danvers, MA, USA) and incubation with the Luminata forte western HRP substrate (Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... 50 mM β-glycerophosphate) and 6 μL of anti-pS6 antibody (Cell Signaling D68F8) were added ...
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bioRxiv - Microbiology 2023Quote: ... Rabbit monoclonal antibodies against 6-His-tags (12698S) were purchased from Cell Signaling Technology. The rabbit monoclonal anti-histone H2A ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole; Cell Signaling Technology, USA), and finally samples were mounted with ProLong® Gold Antifade Reagent (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... rabbit anti-GATA1 (NF that was produced against recombinant N-terminal zinc finger76, rabbit anti-GAPDH (14C10, Cell Signaling) and anti-β3 integrin (sc-14009 ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were incubated with primary antibodies (PHOX2B: 1:1000, ab183741, abcam; n-Myc: 1:500, 51705 Cell Signaling Technology; CD45 ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue (n=3 tendon per sample) was homogenized in RIPA buffer (including 1X protease/phosphatase inhibitor, Cell Signaling #58725) to extract the total protein with the Biomasher II system and Powermasher II electric stirrer (Nippi Inc ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... The primary antibodies include anti-N-MYC (1:160 dilution, clone D4B2Y, Cell Signaling Technology, 51705; citrate antigen retrieval) and anti-FOXP3 (1:50 dilution ...
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bioRxiv - Cancer Biology 2019Quote: ... Phospho-eIF2α (Ser51) (D9G8) XPTM Rabbit mAb (Cell Signaling Technology Cat# 3398, RRID:AB_2096481, Lot# 6), PERK (D11A8 ...
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bioRxiv - Immunology 2023Quote: ... These tissue samples were washed extensively (6 times) with RBC lysis buffer (Cell Signaling Technology) until the soret absorption peak on the UV-visible spectra was almost negligible and the washes were colorless ...
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bioRxiv - Systems Biology 2019Quote: ... A fluorescent labelled secondary antibody conjugated with Alexa Fluor 647 as wells as Alexa Fluor 488 N-Hydroxysuccinimid (NHS, 1-Hydroxy-2,5-pyrrolidindion) and Hoechst-33342 staining solution was purchased from Cell signaling/Life Technologies (Thermo Fischer Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The primary antibodies for N-cadherin (13116T) and E-cadherin (3195T) were purchased from Cell Signaling Technology (Danvers, MA, USA). Primary polyclonal antibodies against MMP9 (E-AB-10063 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were then incubated O/N with anti-Phospho-Gsk3β (Ser9 - D85E12) rabbit monoclonal antibody (1:1.000, cat#5558, Cell Signaling), anti-GSK-3β (3D10 ...
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bioRxiv - Cell Biology 2023Quote: ... Following nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA), larvae were mounted in 80% glycerol between two coverslips and imaged using fluorescence confocal microscopy ...
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bioRxiv - Physiology 2022Quote: ... The primary antibodies used were the following: anti-cleaved Casp3 (catalog n°9661, 1/500, Cell Signaling Technology, Danvers, MA, USA), anti-Smpd3 (Merck clone 14G5.1 ...
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bioRxiv - Immunology 2020Quote: ... Staining was performed as described previously31 using a primary antibody specific for a linear epitope of the N-term portion of GFP (clone D5.1, Cell Signaling Technology) or for a conformational epitope of GFP to detect correctly folded GFP (clone 3E6 ...
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bioRxiv - Neuroscience 2023Quote: 25 mg cortical tissues were dissected from wild type mice (n = 3) and chromatin was prepared using the SimpleChIP plus Enzymatic Chromatin IP Kit (Cell Signaling). UBE3A-bound chromatin was immunoprecipitated using rabbit anti-UBE3A antibody (Bethyl Laboratories) ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.3% Triton X-100), incubated in rabbit anti-phospho-Histone H3-S10 at 1:2000 overnight (O/N, 4°C) (Cell Signaling 3377S), washed 8X in PBSTx (1X PBS plus 0.3% Triton X-100 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells then underwent immunocytochemical processing similar to that described above using primary antibodies against activated c-Jun-N-terminal kinase (rabbit anti-pJNK, 1:100, Cell Signaling Technology) or phosphorylated p38 MAPK (rabbit anti-p-p38 ...
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bioRxiv - Systems Biology 2020Quote: ... Figure S5 shows the knockdown efficiency of siRNA transfections in each cell line with GAPDH siRNA compared to no siRNA (just N-TER transfection reagent) and negative control siRNA by Western blot (GAPDH antibody: Cell Signaling Technology, Cat#2118 ...
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bioRxiv - Cell Biology 2019Quote: ... Cyclin A2 (BF683) (#6; 1:1000) and Phospho-Chk1Ser345 (133D3) (#15; 1:1000) were obtained from Cell Signaling. HA (#SJ254200 ...
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bioRxiv - Cell Biology 2021Quote: Cells in 8-well chamber slides were incubated for 6 hours with 50 nM LMB (Cell Signaling Technology) or with ethanol as control ...
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bioRxiv - Cell Biology 2021Quote: Cells cultured on 6-well plates were stained using SA-β-gal activity assay kit (Cell Signaling Technology) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with syntaxin-6 antibody (C34B2) Rabbit mAb 2869 (Cell Signaling Technology Europe B.V, 2869S; 1:100) for 16Lh at 25L°C with gentle agitation.
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bioRxiv - Microbiology 2022Quote: ... The fluorescent-labelled Alexa Fluor 647 (AF647) goat anti-Rabbit IgG monoclonal antibody was from Cell Signaling Technologies (MA, USA; Cat. n° 4414).
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bioRxiv - Developmental Biology 2021Quote: Protein lysates from 28 hpf control and ncl-/- zebrafish were used (n = 25 per biological replicate; total three biological replicates) for pre-conjugation with Mdm2 antibody (Cell Signaling Technology, #86934) and IgG with magnetic beads ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... and blocked with 5% skimmed milk in TBST for 1 h followed by O/N incubation at 4 °C with appropriate primary antibody: pSTAT3 (D3A7; Cell Signaling Technology, 9145S) and STAT3 (124H6 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit monoclonal anti-SorLA antibody specific to the N-terminus of human SorLA protein (1:1,000; D8D4G #79322; Cell Signaling, Danvers, MA, USA) for the detection of SorLA759 proteins ...
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bioRxiv - Molecular Biology 2022Quote: Synchronized young adult animals expressing N-terminal HA-tagged PMK-1 were used for immunoprecipitation with anti-HA-Tag (C29F4) rabbit mAb (Cell Signaling Technology 11846) and eluted with 2x laemmli buffer ...
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bioRxiv - Physiology 2022Quote: ... for 1 hour at RT and then incubated O/N with primary antibody for c-Fos (Rabbit anti-cFos, Cell Signaling, Cat# 2250) in blocking solution at RT ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained for 1hr in permeabilization buffer with Myc antibody (Myc/n-Myc D3N8F Rabbit mAb PE Antibody, #35876, Cell Signaling, 1:50). Myc expression (expressed as geometric MFI ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated with an appropriate HRP-conjugated secondary antibody (Cell signalling technologies, cat. n. 7074) and LumiGLO® ECL kit (Cell Signaling Technology) was employed following manufacturer’s instruction before to exposure to X-ray film (CytivaTM) ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated with anti-human N-Myc rabbit monoclonal antibody (#51705, Cell Signaling Technology, Inc., MA, USA at a dilution of 1:300) and S100 beta (EP32 ...
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bioRxiv - Cancer Biology 2021Quote: G6PD enzyme activity was determined using the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit (12581S, Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was carried out by using 4’,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Four different sections with no tissue loss were counted for the quantification of cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclear staining was carried out by using 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Larvae were imaged using fluorescence confocal microscopy.
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bioRxiv - Cancer Biology 2020Quote: ... 3127, 1:1000), anti-Jak1 (6G4, 3344, 1:1000), and anti-N-Cadherin (D4R1H, XP 13116, 1:1000) from Cell Signaling Technology (Danvers, MA); anti-phospho-Pak1-2-3 (pSer141 ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated overnight at 4°C with the primary antibodies (Mettl3 Abcam ab195352 1/200; N-Myc Cell Signaling Technology D1V2A 1/200). EU fluorescence coupling was performed following the manufacturer ’s instructions for Click-iT™ RNA Alexa Fluor 488 Imaging Kit ...
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bioRxiv - Biochemistry 2020Quote: ... at 1:1,000 dilution in blocking buffer or glucose 6-phosphate dehydrogenase (G6PDH) antibodies (8866, Cell Signaling Technology, 1:1,000). Immunoreactive species were detected with antibodies conjugated with horseradish peroxidase (715-035-150 ...
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bioRxiv - Cancer Biology 2022Quote: ... their nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, 0.5 ug/ml, 4083S, Cell Signaling Technology, MA, USA) and imaged by using an LSM 880 laser scanning confocal microscope (Carl Zeiss AG ...
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bioRxiv - Cancer Biology 2022Quote: Senescence was assessed using the Senescence β-Galactosidase Staining Kit at pH 6 following the manufacturer’s instructions (Cell Signaling Technology). For SAHF staining ...
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bioRxiv - Neuroscience 2023Quote: ... and were then incubated with 25 μg/mL of DAPI (4’,6-diamidino-2-phenylindole dihydrochloride; Cell Signaling Technology, USA) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were sacrificed 6 days after noise exposure and whole cochlear lysates were prepared in lysis buffer (9803; Cell Signaling) after adding protease (cOmplete ULTRA Tablets 05892791001 ...
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bioRxiv - Cancer Biology 2021Quote: ... 100ug of protein was incubated with 6 uL of ubiquitin antibody and protease/phosphatase inhibitors (Cell Signaling Technologies, Danvers, MA, USA) overnight ...
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bioRxiv - Physiology 2019Quote: ... The supernatant was transferred to a new 1.5 mL lobind tube and mixed with 6 μL pS6 antibody (Cell Signaling, D68F8). Antibody binding was allowed by incubating the mixture for 1.5 hour at 4°C with rotation ...
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bioRxiv - Physiology 2023Quote: Expression of specific proteins in polysomal profiles (Fig. 6) was determined by probing the membrane with antibodies against RsL7a (dilution 1:4000) from Cell Signaling; 4EBP (dilution 1:4000) ...
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bioRxiv - Physiology 2022Quote: ... Isolated islets and MIN-6 cells were mechanically homogenized in ice cold RIPA lysis buffer (Cell Signaling Technology, Danvers, MA, USA) supplemented with 1x protease inhibitors cocktail (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... following incubation with the primary antibodies anti-p53 (1:500, mouse, (Ab-6) Pantropic OP43 Sigma-Aldrich) and anti-GAPDH (1:1000, rabbit, 14C10 Cell Signaling) for 1h at room temperature ...
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bioRxiv - Genetics 2021Quote: MCF7 cells were grown in 6 well plates for Western blots and were washed with cold PBS twice and scraped in RIPA buffer (Cell signaling, USA). Protein concentration in each sample was measured through Bradford assay using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... and Samples were washed extensively in PBS-Tx (6 × 1 hour) and incubated with anti-rabbit-HRP (Cell Signaling #7074, 1:1000) diluted in 1% BSA/PBS-Tx ...