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Citations for Cell Signaling Technology :
1 - 50 of 2268 citations for 4R 5S 2 5 Fluoropyridin 2 yl 4 5 diphenyl 4 5 dihydrooxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were fixed with 4% paraformaldehyde for 5 minutes and blocked in 5% goat normal serum (Cell Signaling Technologies, 5425) for an hour before antibody detection using antibodies listed in Supplementary Tables S5-S6 ...
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bioRxiv - Molecular Biology 2024Quote: ... supernatants were transferred to fresh 2 ml tubes and incubated ON at 4°C with either 5 µl α-H2AK119ub1 (D27C4, Cell Signaling Technology #8240), 15 µl α-H3K27me3 (C36B11 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-FU-D113-#4) using an anti-H3K27me3 antibody (Cell Signaling Technology, Ref: 9733-C36B11). Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 5% BSA in TBST: pMEK1/2 (Cell Signaling Technology, Danvers, MA, USA, 41G9, 1:1000), pERK1/2 (Cell Signaling Technology ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was quenched by 3% hydrogen peroxidase prior to 4-hour incubation at 4°C with 5% normal goat serum (Cell Signaling Technology®, Danvers, MA) and rabbit anti-DSG1 (clone EPR677766(B) ...
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bioRxiv - Molecular Biology 2022Quote: ... α-tubulin (1:1000, incubated overnight in 5% BSA in 1 X PBST at 4°C, Cell Signaling), HA-HRP (1:6000 ...
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bioRxiv - Cancer Biology 2022Quote: ... the lysates were incubated at 4°C for 5 h with antibodies for BRD4 (Cell Signaling Technology; 1:100). Antibody/antigen complexes were recovered using Dynabeads Protein A/G (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-4′,6-diamidino-2-phenylindole (DAPI) (Cell Signaling #4083). The primary microglial yield was approximately 5 × 105 cells/flask with minimal astrocyte contamination ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking with 5% swine serum (Jackson Immuno Research, #014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100 ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were then washed with PBS and blocked for 2 hours using TBST + 5% NGS (Cell Signaling). Slides were then exposed to anti-XPO1 (1/200 ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C in 5% BSA/TTBS containing rabbit anti-STING (0.058 µg/ml, Cat. # 13647, RRID: AB_2732796; Cell Signaling Technology), anti-phospho-TMEM173/STING S366 (1 µg/ml ...
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bioRxiv - Physiology 2023Quote: ... blocked with 5% goat serum and incubated overnight at 4 °C with anti-TMEM16A (Cell Signaling, Rabbit D1M9Q 1:100) and CD31 (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... anti-pSmad 1/5/9 (ref: 13820S) and anti-pSmad 2 (ref: 18338S) antibodies were obtained from Cell signaling. The secondary antibody conjugated to Alexa555 or Alexa488 were purchased from Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking tissue with 5% swine serum (Jackson Immuno Research, # 014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were blocked for 1 h at room temperature with 5% skim milk and then incubated overnight at 4°C with appropriate primary antibody dilution: anti-STAT1 (1:1000, Cell Signaling), anti-pSTAT1 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... overnight at 4°C and secondary HRP-conjugated anti-rabbit IgG (1:2,000 in 5% nonfat dry milk; Cell Signaling Technology) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was blocked for 1h with 5% BSA in TBS-T followed by overnight incubation at 4°C with antibody (Drosha 1:1000; D28B1, Cell Signaling, in 5 %BSA ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were blocked with 3% BSA in PBS overnight at 4°C and then incubated with a rabbit phospho-SMAD1/5/9 antibody at 1:400 (D5B10, Cell Signaling) and a mouse anti-YAP antibody at 1:200 (63.7 ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref ...
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bioRxiv - Neuroscience 2023Quote: ... immunoblots were probed for anti-rabbit acetylated histone 3 at lysine 9 and histone 4 at lysine 5 (ac-H3K9 and ac-H4K5 respectively, Cell Signaling), anti-rabbit LC3B (Abcam) ...
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bioRxiv - Molecular Biology 2023Quote: ... washed twice in PBS and after centrifugation at 250 x g for 5 min at 4°C resuspended in cell lysing buffer (Cell Signaling Cat# 9803 ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibody incubation of the sample was carried out overnight at 4°C with the following primary antibody dilutions: rabbit anti-pSMAD1/5/9 1:200 (mAb #13820, Cell signaling, (Genikhovich et al. ...
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bioRxiv - Neuroscience 2024Quote: ... Revert stain was then removed and then membranes were blocked in 5% BSA for 2 hours at RT and then incubated overnight at 4°C in either anti-NLRP3 antibody (15101S, 1:1000 in 5% BSA, Cell Signaling), anti-NLRC4 antibody (12421 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-pSMAD1/5 (Cell Signaling), anti-SMAD4 (Invitrogen ...
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bioRxiv - Pathology 2022Quote: ... (Cell Signaling 15019S, 1:5) and then with diluted anti-αSMA-Cy3 antibody (Sigma Aldrich ...
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bioRxiv - Pathology 2020Quote: ... Rab 5 (Cell Signaling, #3547S) and N-Cadherin (BD Transduction Labs ...
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bioRxiv - Biochemistry 2024Quote: ... 5-LO (Cell signaling: 3289S), 12/15-LO (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... 5% BSA (Cell Signaling Technology) and primary antibodies were incubated in PBS-0.1% Triton X-100 ...
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bioRxiv - Molecular Biology 2024Quote: ... Tau 4R (Cell Signaling Technology; 30328); anti-GFP (Proteintech ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% milk in PBS-T (0.1% Tween 20) and incubated with rabbit anti-mitofusin 2 (mAb D2D10, Cell Signaling), rabbit anti-mitofusin 1 (mAb 13196S ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858), S6 (Cell Signaling 2217) ...
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bioRxiv - Genetics 2021Quote: ... The following primary antibodies were used at 1:1000 dilution in PBST (0.05% Tween-20) and 5% non-fat dry milk and incubated overnight at 4°C: Myc (Cell Signaling Technology #2276), RBPJ (Cell Signaling Technology #5313) ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074; Cell Signaling, Switzerland) and goat anti-mouse antibody (7076 ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated overnight at 4°C with a 1:1000 dilution of rabbit anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies) in block ...
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C with the following primary antibodies diluted 1:1000 in 5% BSA/TBST: anti-pro-IL-1β (#12703, Cell Signaling Technology) and anti-NLRP3 (AG-20B-0014-C100 ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Astrocyte activation to a neurotoxic phenotype was induced in cells at 4-5 DIV by incubation with TNFα (25 ng/ml; Cell Signaling Technology), IL-1α (3 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... + 5% milk and then incubated overnight at 4°C with the following primary antibodies in TBS+5% milk: anti-RPB1 (1:1000; Cell Signaling, 14958), anti-EZH2 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then cut and incubated with different primary antibodies diluted in 5% milk at 4°C overnight: αH3K27me3 (Cell Signaling C36B11) was used at 1:5000 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... Magnetic beads were allowed to bind with 5 μg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) by incubating for 8 hours at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitation was carried out overnight at 4 °C using 5 μl of rabbit polyclonal FosB antibody (2251S, lot 3, Cell Signaling Technology) or 2 μg of rabbit polyclonal IgG isotype (3900S ...
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bioRxiv - Pathology 2021Quote: ... Sections were developed for 5 minutes with DAB Chromagen (Cell Signaling Technology®, 11724/5) before being counterstained with hematoxylin ...
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bioRxiv - Biophysics 2021Quote: ... and phospho-myosin (rabbit phospho-myosin light chain 2 (Thr18/Ser19) antibody (dilution 1:50 in PBS 0, 5% BSA, #3674, Cell Signaling) for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T, #9102, Cell Signaling) and phospho-p44/42 MAPK (Thr202/Tyr204 ...
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bioRxiv - Plant Biology 2023Quote: ... Blots for detecting phosphorylated MAPK were blocked in 5% BSA and immunoblotted with a phosphor-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling). Horseradish peroxidase-conjugated anti-rabbit antibody produced in goat (Agrisera ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% non-fat dry milk for 2 h at RT and incubated overnight at 4°C with rabbit anti-phospho-ERK1/2 (1:2000, Cell Signaling), anti-phospho-Ser473-Akt (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was then probed with the following primary antibodies diluted in 5 % BSA /PBS-T for overnight at 4 °C: β-actin (1:1000; Cell Signaling Technology), ZO-1 (1.25 μg/ml) ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Cells were fixed with 4% paraformaldehyde for 5 min and subsequently permeabilized with 0.2% Triton-X and stained with anti-relB (Cell Signaling Technology, Danvers, USA) followed by goat-anti-rabbit Alexa 546 (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated overnight at 4°C with the primary antibody mAb c-MYC (dilution 1:1000 in TBST/5% milk; Cell Signaling Technology, D84C12). Subsequently ...