Human High Sensitive Leucine Rich Alpha 2 Glycoprotein 1 LRG1 CLIA Kit citations for Cell Signaling Technology

Activated PI3Kδ syndrome, an immunodeficiency disorder, leads to sensorimotor deficits recapitulated in a murine model

Ines Serra, et al., bioRxiv - Neuroscience 2021

Quote: ... for 1 h and subsequently incubated with anti-PI3K p110δ (D1Q7R) Rabbit mAb (1:1.000, #34050, Cell Signaling Technology, B.V.) in 5% BSA-TBS with 0,1% Tween 20 (TBS-T ...

Low dose rate γ-irradiation protects Drosophila melanogaster chromosomes from double strand breaks and telomere fusions by modulating the expression of the esi-RNA biogenesis factor Loquacious

A. Porrazzo, et al., bioRxiv - Genetics 2021

Quote: ... The samples were incubated with the anti-Cleaved Drosophila Dcp-1 (1:50; Rabbit; Asp216 #9578; Cell Signaling Technology®) overnight at 4°C ...

The X-linked splicing regulator MBNL3 has been co-opted to restrict placental growth in eutherians

Thomas Spruce, et al., bioRxiv - Evolutionary Biology 2021

Quote: ... Membranes were incubated overnight with MBNL3 antisera 41 at 1:500 or anti Lamin B1 (1:1000, Cell Signaling Technologies) in 5% non-fat dairy milk in PBS plus Igepal (0.05% ...

On the therapeutic potential of MAPK4 in triple-negative breast cancer

Fadia Boudghene-Stambouli, et al., bioRxiv - Cancer Biology 2022

Quote: ... from Santa Cruz Biotechnology; anti-phospho-AKT(Thr308) (1/1,000; #13038) and anti-phospho-AKT(Ser473) (1/1,000; #4051) from Cell Signaling Technology.

NFAT5 induction by the tumor microenvironment enforces CD8 T cell exhaustion

Laure Tillé, et al., bioRxiv - Immunology 2022

Quote: ... Whole cell extracts were isolated using RIPA lysis buffer (25mM Tris HCl pH 7.6, 150mM NaCl, 1% NP 40, 1% sodium deoxychlorate, 0,1% SDS; Cell Signaling Technology) supplemented with DNase ...

Cholesterol biosynthesis inhibition synergizes with AKT inhibitors in triple-negative breast cancer

Alissandra L. Hillis, et al., bioRxiv - Cancer Biology 2024

Quote: ... cells were incubated with primary antibody for 1 hour rocking at room temperature (LAMP1; Cell Signaling Technology, 9091, 1:100). Cells were washed three times with 1X PBS and then incubated with secondary antibody for 30 minutes rocking at room temperature (Goat anti-Rabbit IgG Alexa Fluor 488 ...

The Alk receptor tyrosine kinase regulates Sparkly, a novel activity regulating neuropeptide precursor in the Drosophila CNS

Sanjay Kumar Sukumar, et al., bioRxiv - Developmental Biology 2024

Quote: ... Primary antibodies used were: guinea pig anti-Spar (1:1000) (this study) and anti-tubulin (Cell Signaling #2125, 1:20,000). Secondary Antibodies used were ...

A whole-genome CRISPR screen identifies the spindle accessory checkpoint as a locus of nab-paclitaxel resistance in pancreatic cancer cells

Priya Mondal, et al., bioRxiv - Cancer Biology 2024

Quote: Cells were harvested post-treatment and re-suspended in lysis buffer (50⍰mM Tris-HCl pH 7.4, 150⍰mM NaCl, 1% NP-40, 1% protease inhibitor cocktail (Cell Signaling), sonicated ...

A single amino acid in the Salmonella effector SarA/SteE triggers supraphysiological activation of STAT3 for anti-inflammatory target gene expression

Margaret R. Gaggioli, et al., bioRxiv - Molecular Biology 2024

Quote: ... beads were washed three times in 1 mL Lysis Buffer and twice in 1 mL 1x Kinase Buffer (Cell Signaling) supplemented with Phosphatase Inhibitors (PhosSTOP ...

TFEB degradation is regulated by an IKK/β-TrCP2 phosphorylation-ubiquitination cascade

Yan Xiong, et al., bioRxiv - Cell Biology 2023

Quote: ... Histone H3 (Cat #9715S) (1:30,000) and IKKγ Kinase (DA10-12) (Cat# 2695T) (1:1000) were from Cell Signaling Technology; antibodies to IKKβ Kinase (Cat# ab124957 ...

The SOCS1 KIR and SH2 domain are both required for suppression of cytokine signaling in vivo

Karen Doggett, et al., bioRxiv - Cell Biology 2022

Quote: Anti-phospho-STAT1 (CST#7649; 1:1000) and anti-STAT1 (9172; 1:1000) antibodies were purchased from Cell Signaling Technology. Anti-phospho-JAK1 (7000028 ...

HSV-1 miRNAs are posttranscriptionaly edited in latently infected human ganglia

Andreja Zubković, et al., bioRxiv - Microbiology 2023

Quote: ... slides were incubated with rabbit monoclonal α-ADAR1 IgG (Cell Signaling Technology; diluted 1:100 in 1% BSA in PBS), mouse monoclonal α-ADAR1 IgG (Santa Cruz ...

Mutational impact of APOBEC3A and APOBEC3B in a human cell line and comparisons to breast cancer

Michael A. Carpenter, et al., bioRxiv - Cancer Biology 2023

Quote: ... and anti-ý-Actin (Millipore Sigma, mouse mAb, 1:5000 or Cell Signaling Technology, rabbit mAb, 1:5000, as indicated). The secondary antibodies used were anti-rabbit HRP-linked (CST 7074 ...

3D Chromatin Dynamics during Innate and Adaptive Immune Memory Acquisition

Endi K. Santosa, et al., bioRxiv - Immunology 2023

Quote: ... cells were washed twice with Buffer 1 and resuspended in 50ul of Buffer 1 + 1X pA/G-MNase (Cell Signaling) and incubated on ice for 1 hr and washed twice with Buffer 2 (0.05% w/v Saponin ...

Prime editing of the β₁ adrenoceptor in the brain reprograms mouse behavior

Desirée Böck, et al., bioRxiv - Genetics 2023

Quote: ... Brain sections were incubated with primary antibodies overnight at 4°C (mouse-NeuN, 1:500, abcam ab177487; rabbit-Cas9, 1:1,000, Cell Signaling clone D8Y4K ...

Inferring a role for programmed cell death during the origin and evolution of wing polyphenism in ants

Lisa Hanna, et al., bioRxiv - Evolutionary Biology 2024

Quote: The following primary antibodies were used for immunohistochemistry: anti-cleaved caspase-3 (1:100-1:200, Cell Signaling Technology, #9661) and anti-LC3A/B (1:100 ...

Diptera-specific Daedalus controls Zucchini endonucleolysis in piRNA biogenesis independent of exonucleases

Yuica Koga, et al., bioRxiv - Biochemistry 2024

Quote: ... the membranes were incubated with peroxidase-conjugated anti-mouse IgG antibodies (1:5,000 dilution, 55558, Cappel) or anti-rabbit IgG antibodies (1:5,000 dilution, 7074, Cell Signaling Technology). The membranes were then reacted with Clarity Western ECL Substrate (Bio-Rad ...

Variants in ALDH1A2 reveal an anti-inflammatory role for retinoic acid and a new class of disease-modifying drugs in osteoarthritis

Linyi Zhu, et al., bioRxiv - Molecular Biology 2021

Quote: ... Anti-phospho-SAPK/JNK antibody (#9251) and anti-phospho-p44/42 MAPK (Erk1/2) antibody (#9101) were obtained from Cell Signaling Technology (MA, USA). The PPARγ inhibitor ...

Cytosolic pH controls fungal MAPK signaling and pathogenicity

Tânia R. Fernandes, et al., bioRxiv - Microbiology 2022

Quote: ... Phosphorylation of Mpk1 and Fmk1 MAPKs was detected using rabbit anti-Phospho-p44/42 MAPK (Erk1/2) antibody (Thr202/Tyr204, #4370; Cell Signaling Technology, Danvers, MA). Phosphorylation of Hog1 MAPK was detected using rabbit anti-Phospho-p38 MAPK antibody ((Thr180/Tyr182 ...

Missense mutations in the calcium-activated chloride channel TMEM16A promote tumor growth by activating oncogenic signaling in Human Cancer

Silvia Cruz-Rangel, et al., bioRxiv - Cancer Biology 2023

Quote: ... pEGFR (Y1068, #3777) pAKT (S 473, #9271 and p44/42 MAPK (ERK1/2) (T202/Y204, #4370) were purchased from Cell Signaling Technology (Danvers, MA), β- actin was used as a housekeeping proteinand the antibody was bought from EMD Millipore (#MAB1501 now part of Millipore Sigma ...

Elevated CO₂ alters soybean physiology and defense responses, and has disparate effects on susceptibility to diverse microbial pathogens

Melissa Bredow, et al., bioRxiv - Plant Biology 2024

Quote: ... and MAPK activation was assessed by immunoblot analysis using primary anti-Phospho-p44/42 MAPK antibody (Erk1/I 2; Thr-202/Tyr-204) (Cell Signaling, Danvers, MA, USA) and secondary goat anti-rabbit-HRP antibody (Cell Signaling ...

Continuous exposure to 60 Hz extremely low frequency electromagnetic field at 10 to 16 mT promotes various human cell proliferation by activating extracellular-signal-regulated kinase

Jaeseong Goh, et al., bioRxiv - Cell Biology 2024

Quote: ... The membrane was incubated at 4°C overnight with the following primary antibodies: anti-phospho-MEK1/2 (Cell Signaling Technology, Inc., MA, USA, #9122), anti-MEK1/2 (Santa Cruz biotechnology ...

SARS-CoV-2 ORF6 disrupts nucleocytoplasmic transport through interactions with Rae1 and Nup98

Amin Addetia, et al., bioRxiv - Microbiology 2020

Quote: ... 1:500 anti-mCherry (Cell Signaling; clone E5D8F), and 1:1,000 anti-alpha Tubulin (Cell Signaling ...

PIP₂-dependent coupling of voltage sensor and pore domains in K_v7.2

Shashank Pant, et al., bioRxiv - Biophysics 2021

Quote: ... anti-β-tubulin (Cell Signaling #2146, 1:1000), anti-Kv7.2 (Neuromab ...

Cytidine deaminase protects pancreatic cancer cells from replicative stress and drives resistance to DNA-targeting drugs

A. Lumeau, et al., bioRxiv - Cancer Biology 2021

Quote: ... P-Chk1 S345 (Cell signaling #2348, 1:1000), Chk1 (Cell signaling #2360 ...

Interferon-Gamma Signaling Promotes Melanoma Progression and Metastasis

Bo Zhou, et al., bioRxiv - Cancer Biology 2021

Quote: ... pSTAT1 (Y701, 58D6, 1:1,000; Cell Signaling Technology), pSTAT1 (Y727 ...

Interferon-Gamma Signaling Promotes Melanoma Progression and Metastasis

Bo Zhou, et al., bioRxiv - Cancer Biology 2021

Quote: ... GAPDH-HRP (D16H11, 1:1,000; Cell Signaling Technology), IRF1 (D5E4 ...

Interferon-Gamma Signaling Promotes Melanoma Progression and Metastasis

Bo Zhou, et al., bioRxiv - Cancer Biology 2021

Quote: ... pSTAT3 (Y705, D3A7, 1:2,000; Cell Signaling Technology), GAPDH-HRP (D16H11 ...

TRIM67 drives tumorigenesis in oligodendrogliomas through Rho GTPase-dependent membrane blebbing