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Citations for Cell Signaling Technology :
401 - 450 of 1197 citations for Ethyl 5 4 fluorophenyl 5 oxovalerate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... permeabilised with methanol/acetone (50/50) and then stained intracellularly by incubating cells with rabbit antibody against p-(Y694) STAT-5 (D47E7) XP (Cell Signaling), mouse antibody against BCL-2 (100 ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were stained with polyclonal anti-TCF7L1 antibody raised in rabbit (1:100 for complete slides, 1:25 for TMAs; 28835 clone D15G11 lot 5, Cell Signaling). For TMA analysis ...
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bioRxiv - Genetics 2021Quote: ... containing 5% (w/v) skimmed dried milk powder and immunoblotted using Phospho-(Ser/Thr) ATM/ATR Substrate Antibody (#2851 Cell Signaling) (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies and dilutions (in 5% milk in TBST) were used for western blotting: E-cadherin (#14472, monoclonal mouse, 1:1000, Cell Signaling), N-cadherin (#13116 ...
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bioRxiv - Biophysics 2021Quote: ... and phospho-myosin (rabbit phospho-myosin light chain 2 (Thr18/Ser19) antibody (dilution 1:50 in PBS 0, 5% BSA, #3674, Cell Signaling) for 2 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... and probed with the following antibodies diluted in 5% skim milk/PBS (w/v): anti-HA (1:4000, clone C29F4, Cell Signaling), anti-cMyc (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with either 5% skimmed milk or 5% bovine-serum albumin (BSA) and incubated with dilution of respective antibodies as per manufacturer’s instructions (Cell Signaling technologies). The membrane was washed ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to nitrocellulose using standard methods and membranes were blocked in 5% non-fat dry milk (Cell Signaling Technology) in TBS with 0.2% Tween-20 (Boston BioProducts) ...
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bioRxiv - Cancer Biology 2022Quote: ... De-identified FFPE tumor specimens were cut into 5 μm sections and IHC performed as described above to stain for human EZH2 (5246, Cell Signaling) and NKp46 (AF1850 ...
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bioRxiv - Developmental Biology 2022Quote: ... antigens were retrieved by boiling slides in citrate buffer (pH 6.0) for 1 min and pSMAD1/5 Ab (Cell Signaling, 41D10) was added overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... fresh frozen OCT-embedded embryo lung sections (5 μm thick) were stained with anti-E-Cadherin (Cell Signaling, Cat No 3195), anti-Snail (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... The protein A agarose bead was added with 450 µl of IP buffer and 5 µl of Myc antibody (Cell Signaling). The mixture was then incubated on rotator at 4°C for 4 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were then incubated o/n at 4C with 5% milk powder in PBS-T containing the primary antibodies at the following dilutions (1:1000 Lin28a (A177, Cell signaling); 1:4000 H3 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T, #9102, Cell Signaling) and phospho-p44/42 MAPK (Thr202/Tyr204 ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies (in 5% milk in TBST) were used for western blotting: anti-E-cadherin (#14472, monoclonal mouse, 1:1000, Cell Signaling), anti-N-cadherin (#BDB610920 ...
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bioRxiv - Immunology 2021Quote: ... Cells were treated with 5% goat serum for 30 min and incubated with antibodies against TRPC3 (Alomone Labs) or NFkB-p65 (Cell Signaling) for 1h ...
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bioRxiv - Developmental Biology 2022Quote: ... The soft palatal tissue from E13.5 embryos was collected and fixed according to the manufacturer’s instructions based on Cut and Run assay kit (Cell Signaling, 86652). Ten μl of IgG isotype control (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... The chromatin was then incubated with 5 µg of antibody against KDM6A (A302-374A, Bethyl Laboratories and 33510S, Cell Signaling Technology), KDM6B (ab169197 ...
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bioRxiv - Cancer Biology 2022Quote: β-Galactosidase staining at pH 6.0 was performed with 5 × 105 cells using the Senescence β-Galactosidase Staining kit (Cell Signaling # C10841) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... followed by blocking in 5% BSA with 0.2% Triton X-100 for 30min and incubation with primary antibody Axin1 (#2087, 1:200, Cell Signaling Technology) overnight ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% skim milk and then treated with Trim28 primary and anti–rabbit IgG (Cell Signaling Technology) secondary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with PBS-0.1% Tween 20 (PBS-T) containing 5% milk and were incubated with rabbit monoclonal anti-CYLD (1:500, #8462 Cell Signaling) and mouse monoclonal anti-β-actin (1:5,000 ...
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bioRxiv - Neuroscience 2023Quote: ... and the grey matter was Dounce homogenized in 5 volumes per weight (v/w) buffer containing phosphate buffer saline (PBS) and 1X protease inhibitor (#5871, Cell signaling) in a 15 ml glass homogenizer with 30 up and down strokes at 70% power by hand on ice ...
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bioRxiv - Plant Biology 2023Quote: ... Blots for detecting phosphorylated MAPK were blocked in 5% BSA and immunoblotted with a phosphor-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling). Horseradish peroxidase-conjugated anti-rabbit antibody produced in goat (Agrisera ...
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bioRxiv - Cancer Biology 2023Quote: ... The extract was then incubated with 5 μg of Anti-FLAG Antibody (DYKDDDDK Tag (D6W5B) Rabbit mAb#14793 antibody (Cell Signaling) and 50 μL of protein G beads at 4 ºC overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed 3x 5 mins with PBS and then stained with corresponding secondary antibodies (1:1000; 4414S Cell Signaling Technology, 8889S Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking solution was replaced with 1° antibody (Ab) (Phospho-Smad1/5/9 Rabbit mAb #13820, Cell Signaling Technology 13820T, 1:300) in 10%NGS/PBS-Tx and the slides were incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... All antibodies were diluted in 1X PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membrane was shaken three times for 5 min in TBST then incubated with 1:3,000 anti-rabbit HRP-linked antibody (Cell Signaling Technologies) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked for 1h and probed with 5% nonfat dry milk with the following primary antibodies: anti-IRF8 (clone D20D8, Cat# 5628, Cell Signaling), anti-CD74 (sheep anti-mouse cd74 ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies were diluted 1:1000 in 5% milk/TBST (FGFR1: D8E4; GAPDH HRP-conjugated: 14C10; Cell Signaling, Danvers, MA, USA). The membranes were incubated with antibodies at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... cells were permeabilized with 100% cold methanol and stained with anti-phosphorylated (p)SMAD1/5/9 (Cell Signaling Technology, Danvers MA) overnight at a 1:50 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... were blocked in TBST buffer (Tris-buffered saline and 0.15% Tween-20) containing 5% nonfat dry milk (#9999, Cell Signaling Technology). The blots were probed overnight with primary antibodies diluted in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... Membranes were blocked for 1 h in 5% non-fat milk in 1X TBST at room temperature and incubated with primary antibodies against KEAP1 (#8047, Cell Signaling), NQO1 (#11451-1-AP ...
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bioRxiv - Physiology 2024Quote: ... C2C12’s were replated and allowed 24 hours to recover after which the labeled C2C12 myoblasts were induced for apoptosis using 5 µM Staurosporin (cat. no.: 9953S, Cell Signaling) for 18h ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were then blocked and permeabilized for 1 hour at room temperature while rocking with 1mL of blocking buffer (0.3% Triton X 100 and 5% normal goat serum (Cell Signaling Technology) in 1x PBS ...
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bioRxiv - Physiology 2024Quote: ... Triton X-100 CD31+ and CD31-cell lysates (5-15 µg/lane) were analyzed by western blot for proteins and eNOS (#32027; 1:500; Cell Signaling) and GAPDH (#97166 ...
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bioRxiv - Neuroscience 2024Quote: ... washed in TBST-Tween20 and blocked for 1h at room temperature with TBST/5% Normal Goat Serum (Cell Signaling Cat# 5425). Following blocking ...
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bioRxiv - Molecular Biology 2024Quote: ... Plates were washed three times with PBS-T for 5 minutes each.The wells were then incubated with Mouse Anti-Myc antibody (9B11, Cell Signaling Technology) diluted 1:3000 in PBS-T with 5% BSA for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated with primary antibody anti-phospho-histone H2A.X (γH2AX) (#2577; Cell Signaling. 1:200 in 5% BSA in PBS), for 1 hour ...
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bioRxiv - Biophysics 2024Quote: ... Knockdown efficiency was validated by immunoblotting with pannexin-1 primary antibody (Cell Signaling Technologies, 91137, 1:1000 in 5% BSA-TBST).
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bioRxiv - Cancer Biology 2024Quote: ... followed by 3 5-minute washes by 1x TBST and probing with horseradish peroxidase conjugated secondary antibodies (Cell Signaling, 7074, 7076) for 1-1.5 hours at 25°C ...
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bioRxiv - Cancer Biology 2024Quote: ... then stained overnight with primary antibodies in blocking buffer (5% goat serum or SignalStain antibody diluent, Cell Signaling Technology, cat#8112). For non-CST primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Transferred blots were blocked for 1 hour in 5% w/v non-fat dry milk in 1X TBST (Cell Signaling Cat# 9997). Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858) ...
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bioRxiv - Cell Biology 2020Quote: For immunoblotting the following primary antibodies were used at a concentration of 1:1000 in 5 % milk-TBS-T: HA (Cell Signaling 3724S), HA (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% milk and incubated in 2% BSA with primary antibodies to GAPDH (Cell Signaling Technology Cat# 13084, RRID:AB_2713924), Eg5 (Sigma Aldrich Cat# SAB4501650 ...
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bioRxiv - Cell Biology 2020Quote: ... the pellet was washed with PBS (–) and incubated on ice for 5 min in 0.1 ml cell lysis buffer (Cell Signaling Technology, Inc.). In the other conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... washed three times with 1x PBS and incubated with 5 % horse serum/1xPBS for 1 h at 20 °C before incubating with the following antibodies (Cell Signaling Laboratories): anti-calnexin (clone C5C9) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibody incubations were done in 5% milk/PBS using rabbit anti-HA at 1:500 (Cell Signaling, Cat #: 3724S) and anti-Rabbit HRP at 1:10,000 (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... After 3 washing steps (5 min each) with PBS-T membranes were incubated with horseradish-linked goat anti-rat (1:2000 dilution; 7077S; Cell Signaling Technology) or goat anti-rabbit (1:2000 dilution ...