1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE citations for Cell Signaling Technology

Quote: ... Pulling down of the pre-cleared chromatin was performed with the use of magnetic beads that were previously incubated with 5 µg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) for 8 hours at 4°C ...

Atmospheric particulate matter aggravates CNS demyelination via TLR-4/NF-κB-mediated microglia pathogenic activities

Bing Han, et al., bioRxiv - Neuroscience 2021

Quote: ... Sonicated lysates were cleared once by centrifugation and incubated overnight at 4°C with magnetic beads bound with NFκB antibody (Cell Signaling Technology, Cat. No. 8242s) to enrich for DNA fragments ...

Rev-erb-α controls skeletal muscle calcium homeostasis through myoregulin repression: implications in Duchenne Muscular Dystrophy

Alexis Boulinguiez, et al., bioRxiv - Cell Biology 2021

Quote: ... 50μg of chromatin were immunoprecipitated overnight at 4°C with an antibody against Rev-erb-α (13418S, Cell signaling Technology, Danvers, Massachusetts, United States). BSA/yeast tRNA-blocked Protein A/G dynabeads (Invitrogen ...

Liver-Chip: Reproducing Human and Cross-Species Toxicities

Kyung-Jin Jang, et al., bioRxiv - Pharmacology and Toxicology 2019

Quote: ... and incubation with the primary antibodies in the blocking buffer for overnight at 4°C was followed by a two-hour incubation with secondary antibodies (Cell Signaling, Danvers, MA, USA) in the blocking buffer at room temperature ...

Extravascular spaces are the primary reservoir of antigenic diversity in Trypanosoma brucei infection

Alexander K. Beaver, et al., bioRxiv - Microbiology 2023

Quote: ... secondary antibody staining was performed for 10 minutes at 4°C with Anti-Rabbit IgG conjugated to Alexa Fluor® 488 fluorescent dye (Cell Signaling Technology, 4412S). Finally ...

Translation-dependent downregulation of Cas12a mRNA by an anti-CRISPR protein

Nicole D. Marino, et al., bioRxiv - Microbiology 2023

Quote: ... and the remaining volume was rotated overnight at 4 °C with 45 μl of anti-C-myc magnetic beads from Cell Signaling Technology (Cat. 5698) or Pierce (Cat ...

HCV infection activates the proteasome via PA28γ acetylation and heptamerization to facilitate the degradation of RNF2, a catalytic component of polycomb repressive complex 1

Hirotake Kasai, et al., bioRxiv - Microbiology 2024

Quote: ... These cells were stained with 50 µM DAPI and incubated at 4 °C overnight in PBS-BSA containing rabbit anti-RNF2 IgG (clone D22F2, Cell Signaling Technology, Denver, MA) and mouse anti-NS5A IgG (clone 9E10 ...

The ubiquitin-conjugating enzyme UBE2D/eff maintains a youthful proteome and ensures protein quality control during aging

Liam C. Hunt, et al., bioRxiv - Cell Biology 2024

Quote: ... The supernatants (Triton X-100 insoluble fraction) were collected and analyzed on 4-20% SDS-PAGE with anti-ubiquitin (Cell Signaling Technologies P4D1, #3936) and anti-Ref(2)P/p62 (Abcam #178840 ...

Meiosis-Specific Cohesin Complexes Display Distinct and Essential Roles in Mitotic ESC Chromosomes

Eui-Hwan Choi, et al., bioRxiv - Molecular Biology 2021

Quote: ... Cells were blocked with 3% BSA in PBS-T for 30 min and then incubated for 1 h with the following primary antibodies (diluted in 1% BSA in PBS-T): anti-RPA (Cell Signaling, #2208, 1:200), anti-Rad51 (Santa Cruz ...

mTORC1 In The Orbitofrontal Cortex Promotes Habitual Alcohol Seeking

Nadege Morisot, et al., bioRxiv - Neuroscience 2019

Quote: ... anti-phospho-CaMKII (T286, 1:1000), anti-phospho-S6K (T389, 1:500) and anti-S6K (1:500) antibodies were purchased from Cell Signaling Technology (Danvers, MA). Anti-CaMKII (1:500 ...

Inactivation of p21-Activated Kinase 2 (Pak2) Inhibits the Development of Nf2-Deficient Malignant Mesothelioma

Eleonora Sementino, et al., bioRxiv - Cancer Biology 2020

Quote: ... 3127, 1:1000), anti-Jak1 (6G4, 3344, 1:1000), and anti-N-Cadherin (D4R1H, XP 13116, 1:1000) from Cell Signaling Technology (Danvers, MA); anti-phospho-Pak1-2-3 (pSer141 ...

Reversal of β-amyloid induced microglial toxicity in vitro by activation of Fpr2/3

Edward S. Wickstead, et al., bioRxiv - Neuroscience 2020

Quote: ... and membranes were blotted using antibodies raised against murine haem oxygenase-1 (HO-1; rabbit polyclonal, 1:1000, Cell Signaling Technology, Leiden, The Netherlands) or superoxide dismutase 2 (SOD2 ...

Downregulation of Stem-loop binding protein by nicotine via α7-nicotinic acetylcholine receptor and its role in nicotine-induced cell transformation

Qi Sun, et al., bioRxiv - Molecular Biology 2022

Quote: ... Antibodies against phosphoylated-AKTT308 (p-AKTT308, 1:500 dilution, #2965), p-AKTS473 (1:500 dilution, #4060), and AKT (1:1000 dilution, #4961s) were from Cell Signaling Technology (Danvers, MA). All other chemicals were of the analytical grade and obtained from the local chemical suppliers ...

FAK drives resistance to therapy in HPV-negative head and neck cancer in a p53-dependent manner

Phillip M. Pifer, et al., bioRxiv - Cancer Biology 2022

Quote: ... 1:1000, #2947), phospho-SAPK/JNK (Thr183/Tyr185, 1:500, #9251), and phospho-CHK2 (Thr68, 1:500, #2661) from Cell Signaling Technology (Danvers, MA); β-actin (C4 ...

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