Labshake search
Citations for Cell Signaling Technology :
351 - 400 of 1028 citations for Testosterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... 1ml (5*106 cells) was aliquoted into each of 3 tubes with antibody (1.25 µg anti-HA, Cell Signaling #3724S ...
-
bioRxiv - Cancer Biology 2023Quote: ... the membrane was blotted overnight with primary antibodies in TBS-T containing 5% BSA (Cell signaling antibodies; Stat1 (9172), phospho-Stat1-Tyr701-(7649) ...
-
bioRxiv - Cell Biology 2023Quote: Cullin-RING E3 ubiquitin ligases were inhibited by addition of 5 μM MLN4924 (Cell Signaling Technology, catalog no.: 85923) and the 26S proteasome was blocked with 10 μM MG132 (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2023Quote: ... anti-pSmad 1/5/9 (ref: 13820S) and anti-pSmad 2 (ref: 18338S) antibodies were obtained from Cell signaling. The secondary antibody conjugated to Alexa555 or Alexa488 were purchased from Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked with 5% nonfat dried milk and then incubated with primary antibodies from Cell Signaling Technology that recognize ...
-
bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) analysis was performed on a QuantStudio™ 5 Real-Time PCR System (Cell Signaling Technology, MA) using the SYBR green reaction mix (Vazyme Biotech ...
-
bioRxiv - Neuroscience 2023Quote: ... The PVDF membranes were blocked with 5% BSA and incubated with rabbit mTOR (1:1000, Cell Signaling Technologies, USA), rabbit p-mTOR (1:1000 ...
-
bioRxiv - Biophysics 2023Quote: ... Each blot was blocked with 10 mL 5% (w/v) nonfat dry milk (Cell Signaling Technology, Danvers, MA, USA) in TBST buffer (25 mM Tris base ...
-
bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% milk and probed using either 1:1000 antiV5 antibody (Cell Signaling Technology, 13202) or 1:10000 GAPDH as a loading control (Proteintech 60004-1) ...
-
bioRxiv - Bioengineering 2021Quote: ... The plates were then washed 3 times with PBST before adding 100 μl of TMB substrate solution (Cell Signaling Technology, USA, Catalog: 7004S) to the plates after aspirating out the PBST and was incubated for 25 minutes at room temperature on a plate shaker ...
-
bioRxiv - Bioengineering 2021Quote: ... A2 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1β (30 ng/ml, Cell Signaling Technology, 8900SF) and TNF-α (30 ng/ml ...
-
bioRxiv - Cancer Biology 2023Quote: Adherent cells were washed in 1x PBS and lysed on the plate by the addition of complete RIPA buffer with protease (Cell Signaling Technology #5871S) and phosphatase (Cell Signaling Technologies #5870S ...
-
bioRxiv - Cancer Biology 2023Quote: ... OV90 and CAOV3 cells were cultured in a glass bottom 96 well plate as described and stained with Cleaved caspase 3 (CC3) antibody (Cell signaling technology: 9661T) at 1:100 dilution after 12 hours incubation with T cells for CAOV3 and 48 hours incubation for OV90 cells ...
-
bioRxiv - Neuroscience 2024Quote: ... 96-well Meso Scale Discovery assay plates (MSD, L15XA-6) were coated with 30 μl of pS65-Ub capturing antibody (Cell Signaling Technology, 62802) with a final concentration of 1 µg/ml in 200 mM sodium carbonate coating buffer (pH 9.7 ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were washed with PBS and then were blocked with 5% (wt/vol) BSA and 0.1% Triton X-100 in PBS before staining with mouse anti-gH2AX (Cell Signaling), rabbit anti-RPA pS4/S8 (Bethyl) ...
-
bioRxiv - Developmental Biology 2020Quote: ... followed by incubation with primary Antibody against pSMAD1/5/8 (the antibody was purchased from Cell Signaling Technology; no. 9511) at 1:200 dilution in blocking solution ...
-
bioRxiv - Molecular Biology 2020Quote: ... BMP signaling was measured using a monoclonal phospho-SMAD 1/5/9(8) antibody (Cell Signaling Technology, Danvers, MA, #13820) at a dilution of 1:1000 ...
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked for 1 h using 5% BSA in TBS-T (0.01% Triton-X) and incubated overnight with primary antibodies (1:500 pSTAT1, 7649 Cell Signaling; 1:500 pSTAT3 ...
-
bioRxiv - Neuroscience 2019Quote: ... diluted with TBS containing 0,1% Tween 20 (TBS-T) and 5% non-fat dry milk: rabbit HRP-conjugated actin 1:10000 (Cell Signaling); rabbit p-JNK and JNK 1:200 (Santa Cruz) ...
-
bioRxiv - Cancer Biology 2019Quote: ... and incubated overnight at 4C with primary antibody diluted in TBS (Fisher) supplemented with 0.1% Tween20 (Fisher) and either 5% BSA (Cell Signaling) or 5% non-fat dry milk (Oxoid) ...
-
bioRxiv - Microbiology 2021Quote: ... Blots were blocked in 0.05% tween-20/5% Non-fat milk in Tris-buffered saline and probed with the indicated antibodies from Cell Signaling: GAPDH (#3683) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Immunohistochemistry was performed on 5-μm thick sections using antibodies against mouse cleaved-caspase 3 (Cell Signaling #9661S, 1:400) and Ki67 (Thermo RM-9106-S ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were washed 10 minutes in 0.2% Triton X-100 and blocked for 1 hour in 5% NDS and 0.05% Triton X-100 in PBS before incubation with rabbit anti Smad3 (1:250, #9624, Cell Signaling), mouse anti PCBP1 (1:200 ...
-
bioRxiv - Biochemistry 2021Quote: ... incubation with a horseradish peroxidase-coupled anti-mouse lgG (Cell Signaling Technology; 1:1000 in TBST with 5% (w/v) non-fat dry milk) ...
-
bioRxiv - Developmental Biology 2019Quote: ... 10 μm cryosections were incubated overnight at 4°C with an antiphosphoSmad1/5/8 antibody (1:500; Cell Signaling 9511S) in PBS with 2% horse serum and 0.1% Triton X-100 ...
-
bioRxiv - Molecular Biology 2019Quote: ... 5 μg of antibody (anti-PUM1, Santa Cruz sc-135049; anti-PUM2, Santa Cruz sc-31535; anti-RBMX Cell Signaling #14794 ...
-
bioRxiv - Molecular Biology 2019Quote: ... BMP signaling was measured using a monoclonal phospho-SMAD 1/5/9(8) antibody (Cell Signaling Technology, Danvers, MA, #13820) at a dilution of 1:1000 ...
-
bioRxiv - Plant Biology 2019Quote: ... Detection of phosphorylated proteins was performed in blocking solution (5% BSA, 0.1% [v/v] Tween-20) with anti-phosphothreonine antibodies (9381, Cell Signaling) and with anti-rabbit-HRP (W4011 ...
-
bioRxiv - Physiology 2021Quote: ... Rabbit polyclonal antibodies to Phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling Technology, 9211S, 1:2500 in 5% BSA for WB); Rabbit polyclonal antibodies to iNOS (Santa Cruz Biotechnology ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and incubated in 5% non-fat dry milk in 1× PBS and 0.1% Tween 20 with the primary antibodies (PHC1 Cell Signaling #13505 1:1000 ...
-
bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% milk in PBS-T (0.1% Tween 20) and incubated with rabbit anti-mitofusin 2 (mAb D2D10, Cell Signaling), rabbit anti-mitofusin 1 (mAb 13196S ...
-
bioRxiv - Cell Biology 2022Quote: ... washed three times with PBS and blocked for 1h in PBS containing 5% normal goat serum (Cell signaling, Cat # #5425) and 0.3% Triton X-100 ...
-
bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in 5% milk before probing with the following antibodies: rabbit anti-SHIP1 (1:1000, Cell Signaling D1163) and rabbit anti-β actin (1:2500 ...
-
bioRxiv - Biophysics 2023Quote: ... blots were incubated in blocking buffer (5%(w/v) milk powder (nonfat dry milk, Cell Signaling Technology, Danvers, MA, USA)) in TBST containing 25 mM Tris base ...
-
bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C in 5% BSA/TTBS containing rabbit anti-STING (0.058 µg/ml, Cat. # 13647, RRID: AB_2732796; Cell Signaling Technology), anti-phospho-TMEM173/STING S366 (1 µg/ml ...
-
bioRxiv - Physiology 2023Quote: ... blocked with 5% goat serum and incubated overnight at 4 °C with anti-TMEM16A (Cell Signaling, Rabbit D1M9Q 1:100) and CD31 (Abcam ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% BSA TBST for 1h at 24 prior to incubating with STAT3 (Cat #12640, RRID:AB_2629499, Cell Signaling Technologies) or NF- kB (p65 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Nitrocellulose membrane was blotted with primary antibodies in TBS containing 0.05% Tween and 5% BSA (Cell signaling; p65-RelA (8242), phospho-p65-RelA-Ser536 (3033) ...
-
bioRxiv - Cell Biology 2023Quote: ... and staining was performed after blocking with 5% normal goat serum with anti-Insulin (Linco) and anti-Glucagon (Cell Signaling) antibodies using established protocols ...
-
bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked in 5% milk and incubated with primary antibody: anti-Phospho-IRF3 (Ser 396) (D601M, Cell Signaling), anti-IRF3 (D6I4C ...
-
bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% milk in 1X TTBS and incubated overnight with the appropriate primary antibody (Cell Signaling c-IAP2 58C7 Rabbit mAb #3130 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Immunoprecipitations were carried out as previously described63 on approximately 5 ug of chromatin (minimum 2.5 ug, maximum 8.8 ug) using 5 ul of total RNA Pol II antibody (Cell Signaling D8L4Y). Libraries were prepared using the NEBNext Ultra II DNA Library Kit for Illumina (NEB ...
-
bioRxiv - Cell Biology 2024Quote: ... The membranes were then blocked with 5% non-fat dry milk in Tris-buffered saline (#9997, Cell Signaling Technology, USA) with 0.1% Tween 20 (P1379 ...
-
bioRxiv - Cell Biology 2020Quote: SMC were seeded at a density of 75000/well in 6 well plates and cultured in FGM for 48 h prior to fixation and staining using a commercial β-galactosidase assay (Cell Signaling Technologies #9860) as described previously (41) ...
-
bioRxiv - Neuroscience 2021Quote: Senescence β-Galactosidase Staining Kit (9860, Cell Signaling technology) was applied to measure the senescent monolayer cultured neural progenitor cells according to the manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2019Quote: ... rabbit anti-Phospho-KIT (Try719) antibody (#3391; Cell Signaling), rabbit anti-Green Fluorescent Protein antibody (AB3080P ...
-
bioRxiv - Neuroscience 2020Quote: ... The PathScan®Immune antibody array kit (Cell Signaling) was used according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ChIP was performed using ChIP kit (Cell signaling, 57976s). Briefly ...
-
bioRxiv - Immunology 2020Quote: ... ChIP was conducted using SimpleChIP kit (Cell Signaling Technology) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... ER Stress Antibody Sampler Kit (#9956, Cell Signaling Technology), Sec24C (#14676 ...