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351 - 400 of 1005 citations for Recombinant Human LDLR protein GST tagged since 2020

• Annexin-A1 tripeptide attenuates surgery-induced neuroinflammation and memory deficits through regulation of the NLRP3 inflammasome

  Zhiquan Zhang, et al., bioRxiv - Neuroscience 2020

  Quote: ... Protein loading was assessed using anti-GAPDH (2118S; Cell Signaling). Bands were quantified with Fiji software ...
• Multiomic deep delve of synthesis and secretion processes in a model peptidergic system

  Soledad Bárez-López, et al., bioRxiv - Neuroscience 2022

  Quote: ... and S6 Ribosomal Protein (1:100, Cell Signaling Technology 2317S) in PBS containing 0.3% (v/v ...
• Antisense oligonucleotides against monoacylglycerol acyltransferase 1 (Mogat1) improve glucose metabolism independently of Mogat1

  Andrew J. Lutkewitte, et al., bioRxiv - Physiology 2020

  Quote: ... green fluorescent protein (GFP, Cell Signaling Technologies, 2555, 1:1000), Calnexin (Enzo Life Sciences ...
• Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and Candida stimulation

  Giang T. Nguyen, et al., bioRxiv - Immunology 2021

  Quote: ... Antibodies to phosphorylated proteins include Syk-Y352 (Cell Signaling Technology), and Pyk2-Y402 (Cell Signaling Technology) ...
• Cdc42 couples septin recruitment to the axial landmark assembly via Axl2 in budding yeast

  Pil Jung Kang, et al., bioRxiv - Cell Biology 2023

  Quote: ... and rabbit monoclonal anti-calmodulin binding protein (Upstate Cell Signaling Solutions ...
• Catalpol inhibits HHcy-induced EndMT in endothelial cells by modulating ROS/NF-κB signaling

  Chengyan Wu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... p-p65 protein antibodies were purchased from Cell Signaling Technology and p65 protein antibodies were purchased from Wan Lei Biology ...
• Bat RNA viruses employ viral RHIMs orchestrating species-specific cell death programs linked to Z-RNA sensing and ZBP1-RIPK3 signaling

  Sanchita Mishra, et al., bioRxiv - Immunology 2024

  Quote: ... anti-SARS-CoV-2 N protein (Cell Signaling Technology, 33717). HRP-conjugated secondary antibodies used in this study were Jackson Immuno Research Laboratories anti-rabbit (111-035-047 ...
• Enhanced cardiac mitochondrial biogenesis by nitro-oleic acid remedies diastolic dysfunction in a mouse model of heart failure with preserved ejection fraction

  Marion Müller, et al., bioRxiv - Pathology 2024

  Quote: ... AMP-activated protein kinase (2532, Cell Signaling, Danvers, MA, USA), phosphorylation at serine 293 of pyruvate dehydrogenase (31866 ...
• Missense and nonsense mutations of the zebrafish hcfc1a gene result in contrasting mTor and radial glial phenotypes

  Victoria L. Castro, et al., bioRxiv - Developmental Biology 2022

  Quote: ... P-S6 Ribosomal Protein (S235/236) (Cell Signaling Cat # 4858S) (anti-rabbit ...
• A twin UGUA motif directs the balance between gene isoforms through CFIm and the mTORC1 signaling pathway

  R. Samuel Herron, et al., bioRxiv - Molecular Biology 2022

  Quote: ... rabbit monoclonal anti-S6 ribosomal protein (Cell Signaling Technology, 2217), rabbit monoclonal anti-Phospho-S6 ribosomal protein (Ser240/244 ...
• Genetic silencing of AKT induces melanoma cell death

  Gennie L. Parkman, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Phospho-S6 ribosomal protein (Ser235/235) (4858; Cell Signaling Technology), S6 ribosomal protein (2217 ...
• Contrasting effects of Ksr2, an obesity gene, on trabecular bone volume and bone marrow adiposity

  Gustavo A. Gomez, et al., bioRxiv - Genetics 2022

  Quote: ... phosphor-S6 ribosomal protein 1:1000 (2215, Cell Signaling Technologies), or B-Actin 1:5000 (A1978 ...
• Lysosomal cystine export regulates mTORC1 signaling to guide kidney epithelial cell fate specialization

  Marine Berquez, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-S6 Ribosomal Protein (Cell Signaling Technology, 2217, 1:500), anti-phospho‒4E-BP1 (Ser65 ...
• STING activation of IRF3 is tuned by PELI2 to suppress basal activation and reinforce the anti-viral response

  Christopher Ritchie, Lingyin Li, bioRxiv - Biochemistry 2023

  Quote: ... 30 μL of protein A magnetic agarose (Cell Signaling Technology) was added incubated for an additional 4 h rotating at 4 °C ...
• Reverse genetic screen identifies malaria parasite genes required for gametocyte-to-sporozoite development in its mosquito host

  Chiamaka Valerie Ukegbu, et al., bioRxiv - Microbiology 2023

  Quote: ... Proteins were detected using rabbit α-HA (Cell Signaling Technology) (1:1000) ...
• MAPK-mediated PHGDH induction is essential for melanoma formation and represents an actionable vulnerability

  Neel Jasani, et al., bioRxiv - Cancer Biology 2024

  Quote: ... pS6 Ribosomal protein (S235/236) (Cell Signaling Technologies, Cat. # 4858S), S6 Ribosomal protein (Cell Signaling Technologies ...
• An extra-glycolytic function for hexokinase 2 as an RNA-binding protein regulating SOX10 mRNA translation in melanoma

  Ana Luisa Dian, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-S6 ribosomal protein (Cell Signaling, #2317; 1:1,000 dilution), anti-VCL (Abcam ...
• Modeling mTORopathy-related epilepsy in cultured murine hippocampal neurons using the multi-electrode array

  Anouk M. Heuvelmans, et al., bioRxiv - Neuroscience 2024

  Quote: ... rabbit anti-S6 Ribosomal Protein (1:2000 #2217 Cell Signaling), rabbit anti-TSC1 (1:1000 ...
• IPMK regulates HDAC3 activity and histone H4 acetylation in human cells

  Gregory A. Sowd, et al., bioRxiv - Biochemistry 2024

  Quote: ... with 20uL Protein-G magnetic beads (Cell Signaling Product #9006), rocked overnight at 4C ...
• PCDHGC3 silencing promotes clear cell renal cell carcinoma metastasis via mTOR/HIF2α and lipid metabolism reprogramming

  Lucía Celada, et al., bioRxiv - Cancer Biology 2024

  Quote: ... rabbit anti-S6 ribosomal protein (5G10) (#2217 Cell Signaling Technology) at 1:1,000 dilution ...
• Uncovering the mechanistic basis of intracellular Raf inhibitor sensitivity reveals synergistic cotreatment strategies

  Ethan G. Stoddard, et al., bioRxiv - Cell Biology 2024

  Quote: Protein A Agarose Beads (Cell Signaling Technology, cat. no. 9863)
• Uncovering the mechanistic basis of intracellular Raf inhibitor sensitivity reveals synergistic cotreatment strategies

  Ethan G. Stoddard, et al., bioRxiv - Cell Biology 2024

  Quote: Protein G Agarose Beads (Cell Signaling Technology, cat. no. 37478)
• Apoptotic Extracellular Vesicles (ApoEVs) Safeguard Liver Homeostasis and Regeneration via Assembling an ApoEV-Golgi Organelle

  Bingdong Sui, et al., bioRxiv - Cell Biology 2021

  Quote: ... apoEVs were stained with rabbit anti-human/mouse VAMP3 primary antibody (13640, Cell Signaling Technology, USA), rabbit anti-mouse VAMP4 primary antibody (PA1-768 ...
• PKD-1 Signaling Is Required for the Maintenance of CSCs with Epithelial-mesenchymal Plasticity in Pancreatic Neuroendocrine Tumors

  Yichen Guo, et al., bioRxiv - Cancer Biology 2022

  Quote: ... mouse anti-human/rat CD44 (5640S) and HRP-linked anti-rabbit IgG (7074) (Cell Signaling Technology), CD45 monoclonal Antibody (14-9457-80) ...
• Caspase-8 deficiency induces a switch from TLR3-induced apoptosis to lysosomal cell death in neuroblastoma cell lines

  Marie-Anaïs Locquet, et al., bioRxiv - Molecular Biology 2020

  Quote: ... anti-PARP (46D11) and anti-Bid human-specific all from Cell Signaling Technology (Danvers, MA, USA). Anti-actin-HRP was purchased from Abcam.
• Distinct SARS-CoV-2 sensing pathways in pDCs driving TLR7-antiviral vs. TLR2-immunopathological responses in COVID-19

  Renée M. van der Sluis, et al., bioRxiv - Immunology 2021

  Quote: ... rabbit-anti-human (r-a-h)MyD88 (clone D80F5, 33 kDa, Cell Signaling Technology, cat#4283) was used ...
• NLRP1 inflammasome modulates senescence and senescence-associated secretory phenotype

  Inés Muela-Zarzuela, et al., bioRxiv - Immunology 2023

  Quote: ... p16 INK4A (mouse: ab189034 and human: 18769S) were obtained from Cell Signaling Technology (Beverly, MA, USA). Finally ...
• Air Pollution Aggravates Renal Ischemia-Reperfusion-Induced Acute Kidney Injury

  Talita Rojas Sanches, et al., bioRxiv - Pathology 2023

  Quote: ... followed by overnight incubation at 4uC with primary rabbit anti-mouse/human STING (Cell Signaling #13647), cGAS (Cell signaling #31659) ...
• ERBB4-mediated signaling is a mediator of resistance to BTK and PI3K inhibitors in B cell lymphoid neoplasms

  Alberto J. Arribas, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The primary antibodies used included rabbit monoclonal anti human p-AKT Ser473 (1:1000, Cell signaling), NF-κB/p65 (E379 ...
• Inflammatory CD4/CD8 double positive human T cells arise from reactive CD8 T cells and are sufficient to mediate GVHD pathology

  Nicholas J. Hess, et al., bioRxiv - Immunology 2023

  Quote: ... Immunoblotting was performed by incubating the membranes with anti-human RUNX3 (Mouse, Cell Signaling Tech, MA), anti-human ThPOK (Rabbit ...
• GeTMoR: Simultaneous genomic, transcriptomic, and morphological profiling of rare single cells

  Rishvanth K. Prabakar, et al., bioRxiv - Genomics 2024

  Quote: ... rabbit IgG anti-human vimentin (Vim or V) (clone: D21H3, 9854BC, Cell Signaling, Danvers, MA, USA), and DAPI were also added to the primary incubation buffers ...
• Branched chain α-ketoacids aerobically activate HIF1α signaling in vascular cells

  Wusheng Xiao, et al., bioRxiv - Cell Biology 2024

  Quote: ... human and rat phospho-BCKDH-E1α (Ser293) (cat # 40368) antibodies were purchased from Cell Signaling Technologies; human COL1A1 (cat # NBP1-30054) ...
• RIPK1 is essential for Herpes Simplex Virus-triggered ZBP1-dependent necroptosis in human cells

  Oluwamuyiwa T. Amusan, et al., bioRxiv - Cell Biology 2024

  Quote: ... Primary antibodies were used at the following dilutions: phosphorylated human MLKL (91689, Cell Signaling, 1:1000), phosphorylated murine MLKL (37333 ...
• AalpiRNA-18529 regulates vitellogenesis of Aedes albopictus via the Gadd45a-mediated JNK-dependent nurse cell apoptosis pathway

  Lu Yang, et al., bioRxiv - Developmental Biology 2024

  Quote: ... an anti-phospho-human-SAPK/JNK (Thr183/Tyr185) rabbit monoclonal antibody (Cell Signaling Technology; Cat#4668), and an anti-β-actin rabbit polyclonal antibody ...
• cGAS-activating lupus autoantibody for cancer immunotherapy

  Xiaoyong Chen, et al., bioRxiv - Immunology 2023

  Quote: ... Samples were analyzed by western blot probed with rabbit monoclonal antibody against human cGAS (Cell Signaling, #15102) and HRP-linked goat anti-rabbit IgG (Abcam ...
• In vivo affinity maturation of the HIV-1 Env-binding domain of CD4

  Andi Pan, et al., bioRxiv - Bioengineering 2024

  Quote: ... were coated overnight at 4°C with a rabbit anti-human CD4 antibody (Cell Signaling Technology, 93518) at a concentration of 2 μg/ml in PBS ...
• Dysregulated synaptic gene expression in oligodendrocytes of spinal and bulbar muscular atrophy

  Madoka Iida, et al., bioRxiv - Neuroscience 2024

  Quote: ... The sections designated to be incubated with the human AR antibody (#5153, 1:1000, Cell Signaling Technology) were boiled in 10 mM citrate buffer for 15 min ...
• Heterogeneity of RNA editing in mesothelioma and how RNA editing enzyme ADAR2 affects mesothelioma cell growth, response to chemotherapy and tumor microenvironment

  Ananya Hariharan, et al., bioRxiv - Cancer Biology 2022

  Quote: ... MAVS (Mouse: Santa Cruz Biotechnology Cat# sc-365333, RRID:AB_10844335, Human: Cell Signaling Technology Cat# 3993, RRID: AB_823565), STING (Cell Signaling Technology Cat# 13647 ...
• Oxygen-independent, hormonal control of HIF-1α regulates the developmental and regenerative growth of cardiomyocytes

  Amarylis C.B.A. Wanschel, et al., bioRxiv - Developmental Biology 2023

  Quote: ... GHRH-R (1:1000; LS-C383690, LSBio, for human cells) β-actin (1:6000; 8H10D10, Cell Signaling), PCNA (1:2000 ...
• Lipid droplet protein Perilipin 2 is critical for the regulation of insulin secretion through beta cell lipophagy and glucagon expression in pancreatic islets

  Siming Liu, et al., bioRxiv - Molecular Biology 2024

  Quote: Human islet cells were immunostained for INS (rabbit anti-INS antibody, C27C9, Cell signaling at 1:300) or GCG (mouse anti-GCG antibody ...
• Disruptors of sestrin-MAPK interactions rejuvenate T cells and expand TCR specificity

  Alessio Lanna, et al., bioRxiv - Immunology 2024

  Quote: ... Cells were firstly intracellularly stained with primary rabbit anti-human H2AX antibody (1:100; 2595S; Cell Signaling); and then with secondary anti-rabbit conjugated-AF488 antibody (1:250 ...
• THE FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycle

  Taylar Hammond, et al., bioRxiv - Cell Biology 2024

  Quote: The following primary antibodies were used for human cell lines: HSP90 (1:2000; Cell Signaling Technology, #4877), GAPDH (1:5000 ...
• Mechanosensitive activation of mTORC1 mediates ventilator induced lung injury during the acute respiratory distress syndrome

  Hyunwook Lee, et al., bioRxiv - Molecular Biology 2020

  Quote: ... proteins were visualized using SignalFire Elite ECL Reagent (Cell Signaling Technology) and imaged using a ChemiDoc XRS+ System (Bio-Rad) ...
• Hepatocyte-specific deletion of XBP1 sensitizes mice to liver injury through hyperactivation of IRE1α

  Caroline C. Duwaerts, et al., bioRxiv - Cell Biology 2020

  Quote: ... Proteins were identified using the following primary antibodies (Cell Signaling Technology, Danvers ...
• T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells

  Ritika Tewari, et al., bioRxiv - Cell Biology 2020

  Quote: Antibodies against the indicated proteins were purchased from Cell Signaling Technology: Lck (Cat ...
• Myogenin is an Essential Regulator of Adult Myofibre Growth and Muscle Stem Cell Homeostasis

  Massimo Ganassi, et al., bioRxiv - Developmental Biology 2020

  Quote: ... phospho-S6 ribosomal protein (Ser240/244) (1:2000; #5364; Cell Signaling), Phospho-Akt (Ser473 ...
• Dopamine ameliorates hyperglycemic memory-induced vascular dysfunction in diabetic retinopathy

  Yeon-Ju Lee, et al., bioRxiv - Biochemistry 2022

  Quote: ... Bcl-2-associated X protein (BAX) (1:1000, Cell Signaling Technology), cytochrome c (1:500 ...
• Lifespan can be extended during a specific time window early in life

  G. Aiello, et al., bioRxiv - Cell Biology 2022

  Quote: ... Rabbit S6 Ribosomal Protein (5G10) (1:4000, Cell Signaling, cat. #2217). anti-dS6K (generous gift from Aurelio Teleman 1:3,000) ...
• Cre-mediated deletion of SMARCA5 disrupts pluripotency in mouse embryonic stem cells

  David P. Cook, Barbara C. Vanderhyden, bioRxiv - Cell Biology 2020

  Quote: ... and 30μL Protein G Magnetic Beads (Cell Signaling Technology, cat #9006) were added for 2 hours at 4°C ...
• Role of c-Met/β1 integrin complex in the metastatic cascade

  Darryl Lau, et al., bioRxiv - Cancer Biology 2020

  Quote: ... 50 μL of protein A magnetic bead slurry (73778S, Cell Signaling) were aliquoted per sample and washed with 500 μL of Complete RIPA buffer ...