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Citations for Cell Signaling Technology :
351 - 400 of 8735 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Lipid-bound protein was run on 4-20% SDS-PAGE gel and immunoblotted with His-tag antibody (rabbit monoclonal, Cell Signaling Technology, 2365). Blots were visualized on a Li-Cor Odyssey imaging system ...
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bioRxiv - Cancer Biology 2024Quote: ... and Western blot performed on resultant protein as described by us.[85] Samples were probed using antibody against cleaved caspase 3 (Cell Signaling Technology, #9664) and β-actin (Santa Cruz ...
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bioRxiv - Biochemistry 2024Quote: ... Bound p52 and His-Bcl3-4E proteins were visualized by immunoblotting using anti-NF-κB p100/p52 antibody (Cell Signaling Technology, Cat#4882) and anti-His antibody (Bio Bharati Life Science ...
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bioRxiv - Physiology 2024Quote: ... The following primary antibodies were used to detect respective proteins by incubation overnight at 4°C in 5% BSA: CD44 (#E7k2y, Cell signaling, 1:1000), RHAMM (#E7S4Y ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amount around 20 µg total protein from the lysate was incubated against anti-p300 CBP HAT antibody (Cell Signaling Technology, Cat #: 86377) respectively at 4°C for 90 min ...
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bioRxiv - Biochemistry 2024Quote: ... 1 hour minimum), proteins were incubated with primary antibodies against GFP (Clonetech, 632381) at 1:5000 dilution and β-actin-HRP (Cell Signaling, 12261) at 1:1000 dilution for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA protein assay kit from Cell Signaling Technology. Thirty micrograms of total protein were mixed in a reducing sample buffer and used for mitochondrial apoptosis assay per the kit instruction ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
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bioRxiv - Microbiology 2020Quote: ... of the sample was incubated with 3 μg of antibody (or 3 μg of IgG as control) and 30 μl of protein G magnetic beads (Cell Signaling Technology, Cat. 7008) at 4 °C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000, Cell Signaling Technology, #8954), anti V5-HRP (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Target proteins were detected by various primary antibodies (For phosphor-CREB rabbit mAb, Cell Signaling Technology #9198; For CREB rabbit mAb, Cell Signaling Technology #4820 ...
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bioRxiv - Plant Biology 2023Quote: ... The bead-bound proteins were separated by SDS-PAGE and detected by western blotting with the MBP antibody (Cell Signaling Technology, Danvers, MA, USA), His-tag antibody (Tiangen ...
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bioRxiv - Immunology 2022Quote: ... The expression levels of target proteins were assessed by western blotting (Figure S1) with antibodies against ADA (clone D1P4Y, Cell Signaling Technology, Cat#65184), ADSSL1 (clone 2D12 ...
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bioRxiv - Cancer Biology 2022Quote: ... we validated the presence of the correctly sized tagged MYC protein and absence of wild type MYC by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). We selected homozygous clone number 2 (for N-BioTAP-cMYC ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Genomics 2023Quote: ... The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20 and 5% bovine serum albumin) containing each of the following primary antibodies: dynamin related protein-1 (DRP1, 1 μg/ml, Cell Signaling Technology, Danver, MA), phosphorylated DRP1 (Ser616 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or Alexa Fluor™ 488 dye-conjugated goat anti-rabbit IgG (Cell Signaling Technology, A 11034, 1:500). All mounted sections were imaged using a Zeiss Axio Imager Z1 ApoTome microscope equipped with a Zeiss AxioCam digital camera and AxioVision 4.7 software.
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bioRxiv - Cancer Biology 2023Quote: ... The lysate samples were denatured by mixing with Blue Loading Gel Dye and DTT (Cell Signaling Technology, 7722). Primary antibodies used in this experiment were as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... protein lysis buffer (Cell Signaling) containing a protease inhibitor cocktail was added and the mixture homogenized ...
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bioRxiv - Molecular Biology 2021Quote: ... The same membrane that was used to detect AIRE protein was washes and reused in an incubation with an anti-GAPDH rabbit polyclonal primary antibody (Cell Signaling Technology, Beverly, MA, USA) following incubation with peroxidase conjugated anti-rabbit antibody.
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bioRxiv - Cancer Biology 2020Quote: ... Protein amounts of OP18 and beta-actin were quantified using a primary stathmin polyclonal antibody (1:1000; Cell Signaling Technology, NEB GmbH, Frankfurt/Main, Germany) and a polyclonal beta-actin antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibody in 10 % Donkey Serum in PBS overnight at 4 °C: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb (Cell Signaling Technology, Cat# 5364; RRID:AB_10694233). After HSCs were washed three times with PBS + 0.1 % Tween-20 ...
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bioRxiv - Cell Biology 2021Quote: ... Forty-five μg of protein were resolved in SDS-PAGE and blotted with anti-phospho-eNOS (Ser1177) antibodies (Cell Signaling, Danvers, MA, USA, 1:250), followed by incubation with goat anti-rabbit horseradish peroxidase secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg of protein per lane were subjected to SDS-PAGE and probed with the following primary antibodies: YAP (Cell Signaling Technologies #8418, 1:500), YAP/TAZ (Cell Signaling #4912 ...
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bioRxiv - Physiology 2023Quote: ... we loaded 25 µg protein of each sample to SDS-PAGE gels and incubated the membranes with 1:1000 eIF2α antibody (Cell Signaling Technology #9722, Danvers, MA) or 1:1000 Phospho-eIF2α antibody (Cell Signaling Technology #9721 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were extracted at 3 hours post-treatment and analysed by western blot following overnight incubation with antibodies against the following primary proteins: Vinculin (E1E9V, rabbit, Cell Signaling Technology, 13901, 1:1000), Phosphorylated p44/42 MAPK (ERK1/2 ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... at room temperature for 1 h and then incubated for 16 h with primary antibodies against the ER tracker protein disulfide isomerase (PDI; rabbit mAb #3501, Cell Signaling Technologies, Danvers, MA, USA) and a loading control GAPDH (rabbit mAb #2118 ...
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bioRxiv - Biochemistry 2022Quote: ... Primary antibodies (rabbit insulin antibody: #4590 Cell Signaling Technology, OC (generic amyloid antibody) and rabbit β-actin antibody ...
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bioRxiv - Plant Biology 2023Quote: ... Then 4 µg of primary antibodies (αHA antibody: Biolegend, #901502; αH2A.Z antibody: abcam, ab4174; αH2AK121ub antibody: Cell Signaling Technology, 8240S ...
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bioRxiv - Biochemistry 2021Quote: ... and followed with detection by Neutravidin-DyLight-800 (Pierce 22853) for biotin-ADP ribosylated Ub and anti-Ub-H2A (Cell signaling Technology #8240) for H2A ubiquitylation.
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bioRxiv - Neuroscience 2021Quote: ... The immunodetection of selected proteins was performed overnight at 4 °C with the following antibodies: p62 (Cell Signaling Technology Inc, Danvers, MA, USA, 5114, 1:1000), LC3b (Abcam ab51520 ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were transferred onto nitrocellulose membranes for immunoblotting and then probed with antibodies for phospho-AMPKα (Thr172) and β-Actin (Cell Signaling Technology, Danvers, MA, USA). The membrane was probed with appropriate secondary antibodies and imaged on an Odyssey Imaging System (LICOR ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated in the following primary antibodies for 3 hours at room temperature: phospho-S6 Ribosomal Protein Ser240/244 (rabbit monoclonal, 1:1000 dilution, Cell Signaling Technology, catalog #5364, RRID: AB_10694233), phospho-AKT Ser473 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/μl (500 μg protein) samples were prepared and incubated overnight at 4 °C in primary antibody against acetyl-lysine (Cell Signaling; Cat No. 9814 (1:100)) ...
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bioRxiv - Cell Biology 2023Quote: ... we used either HRP-conjugated protein A or HRP-conjugated protein G (both from Cell Signaling) because of high background from the immunoglobulin bands when we used secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Some slices were additionally incubated for 20 min with the fluorescent DNA dye DRAQ7™ (1:100, Cell Signaling #7406) at RT and washed twice for 10 min in 0.1 M PB (pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: ... S6 Ribosomal protein (5G10, Cell Signaling), BIM (C34C5 ...
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bioRxiv - Molecular Biology 2022Quote: ... protein lysis buffer (Cell Signaling Technology) containing a protease inhibitor cocktail was added ...
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bioRxiv - Physiology 2022Quote: ... and Biotinylated Protein ladder (Cell Signaling) were included on the gels ...
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bioRxiv - Cell Biology 2023Quote: ... one for each protein (Cell Signaling). Each membrane piece was then incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody solution (α-LSD1 antibody, Cell Signaling, 1/100 ...
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bioRxiv - Neuroscience 2020Quote: ... including the primary antibodies (mouse anti-Iba1 antibody, Abcam, 1:400; rabbit anti-iNOS antibody, Abcam, 1:50; mouse anti-GFAP antibody, Cell signaling, 1:400 ...
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bioRxiv - Physiology 2023Quote: ... blots were washed with TBS-T three times and then incubated in TBS-T containing 5% non-fat dry milk and anti-biotin horseradish peroxidase (Cell Signaling, Danvers, MA, USA) and HRP-conjugated donkey anti-chicken antibody (Sigma-Aldrich ...
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bioRxiv - Zoology 2022Quote: ... The sections were then incubated with anti-phosphorylated-p44/42 mitogen-activated protein kinase (MAPK; extracellular regulated kinase, ERK1/2) rabbit monoclonal antibody (#4370, Cell Signaling Technology, Danvers, MA, USA; 1:500), which was used in zebrafish OE36 ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary antibodies (HRP anti-GFP antibody [Abcam ab190584]; rabbit anti-GSK3α antibody [Cell Signaling Technology 4818S] ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were dissociated into single cells by TlypLE select and suspended in PBS containing the DNA dye DRAQ-7 (Cell Signaling). Using FACS Aria IIIu flowcytometry ...