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Citations for Cell Signaling Technology :
301 - 350 of 10000+ citations for Recombinant HIV 1 GP120 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Proteins of interest were detected with HRP-conjugated horse anti-mouse IgG antibody (1:5000, 7076S, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA) or HRP-conjugated goat anti-rabbit IgG antibody (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed on 6% acrylamide SDS-PAGE gels followed by immunoblotting with rabbit anti-myc (1:3000, 71D10, Cell Signaling Technology, 2278S). In vitro dephosphorylation experiments were performed in three biological replicates.
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bioRxiv - Neuroscience 2019Quote: ... Formalin fixed brains were paraffin embedded and analyzed using the following antibodies according to immunohistochemistry protocols described previously (Chakrabarty, 2010; Chakrabarty, 2015): Glial Fibrillary Acidic Protein (GFAP, Cell Signaling, 1:1000) and ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cancer Biology 2019Quote: ... 15 μg of extracted proteins were separated by SDS-PAGE and revealed by antibodies directed against actin (1: 1000, Cell Signaling Technology #8457), GAPDH (1 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit monoclonal anti-SorLA antibody specific to the N-terminus of human SorLA protein (1:1,000; D8D4G #79322; Cell Signaling, Danvers, MA, USA) for the detection of SorLA759 proteins ...
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bioRxiv - Microbiology 2021Quote: ... was used for western blotting at a dilution of 1:1,000 and rabbit polyclonal antiserum reactive against protein kinase A (PKA) substrates (Cell Signaling Technology, Danvers, MA) was used for western blotting at a dilution of 1:1,000 ...
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bioRxiv - Genomics 2023Quote: ... Correct expression of the mAID-Dam-Lamin B1 fusion protein as well as the wildtype Lamin B1 protein was confirmed using Immunofluorescence staining using an anti-HA antibody at 1 in 500 dilution (Cell Signaling Technologies, C29F4) and an anti-LaminB1 antibody at 1 in 500 dilution (Abcam ...
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bioRxiv - Biophysics 2023Quote: ... mAb #3288) and Lysosome Associated Membrane Protein 1 (LAMP1) specific rabbit monoclonal antibody (cat. no. mAb #9091) were purchased from Cell Signaling Technology (USA). Anti-caveolin-1 rabbit polyclonal antibody was acquired from BD Biosciences (USA ...
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bioRxiv - Immunology 2020Quote: ... The same amount of protein was immunoprecipitated with anti-NEMO and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). The IKKα/β kinase activity in the IP complex was tested by using IKKβ Kinase Enzyme Kit (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... that combines the IgG binding domain of both Protein A and Protein G were used to cross-link anti-Kv11.1 antibodies (Cell Signaling). Stock beads with a concentration of 10 mg/ml were washed four times with 10 volumes of Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Biochemistry 2022Quote: Protein A agarose beads (Cell Signaling Technologies 9863S) were washed and incubated in NET150 buffer (20 mM Tris HCl ph ...
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bioRxiv - Cell Biology 2022Quote: ... ribosomal protein S6 (#2217 CST) from Cell Signaling. Mouse monoclonal anti-beta Actin AC-15 HRP (#AB49900 ...
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bioRxiv - Molecular Biology 2021Quote: ... or directed to T6B-fusion protein (Cell Signaling anti-FLAG #8146S ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μl Protein G agarose beads (Cell Signaling) were washed in IP buffer and blocked with bovine serum albumin (BSA) ...
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bioRxiv - Biochemistry 2019Quote: ... When blotting for total proteins (Cell Signaling Technology; EGFR #4267 ...
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bioRxiv - Cell Biology 2020Quote: ... or Protein A HRP conjugate (Cell Signaling Technology) diluted at 1:5000 in blocking buffer for 45 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... S6 ribosomal protein mouse mAB (2317S, Cell Signaling), phospho-p70 S6 kinase (Thr389 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A/G agarose beads (Cell Signaling Technology) were then added and incubated for 2 hr at 4°C ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween and detection of total CHK1 or CHK2 proteins was carried out for 1 h at RT using mouse-anti-Chk1 mAb (2G1D5) (Cell Signaling 2360; Lot 3) or mouse anti-Chk2 mouse mAb (BD Biosciences 611570) ...
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bioRxiv - Neuroscience 2019Quote: ... anti-Microtubule-associated proteins 1A/1B light chain 3B (LC3B-I/-II) at 1:1000 dilution (14, 16 kDa, Cell Signaling Technology, Danvers, MA); anti-monocarboxylate transporter 1 (MCT1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were then precleared by incubation for 1 hour at 4°C while rotating with 50 μL protein A agarose beads (Cell Signaling Technologies, Cat #9863) CCNT1 antibody (Cell Signaling Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS–polyacrylamide gel electrophoresis and the proteins were detected with the following antibodies: LOXL2 (Cell Signaling; 99680S), BRD4 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Protein amounts of OP18 and beta-actin were quantified using a primary stathmin polyclonal antibody (1:1000; Cell Signaling Technology, NEB GmbH, Frankfurt/Main, Germany) and a polyclonal beta-actin antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Forty-five μg of protein were resolved in SDS-PAGE and blotted with anti-phospho-eNOS (Ser1177) antibodies (Cell Signaling, Danvers, MA, USA, 1:250), followed by incubation with goat anti-rabbit horseradish peroxidase secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Plant Biology 2022Quote: ... The extracts were then incubated at 28°C and the samples were collected at the indicated intervals for determination of GST and GST-WL1 protein abundance by western blots using the anti-GST (#2625, Cell Signaling Technology, 1:1000 dilution) and alkaline phosphatase-conjugated anti-rabbit (# 31340 ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg of protein per lane were subjected to SDS-PAGE and probed with the following primary antibodies: YAP (Cell Signaling Technologies #8418, 1:500), YAP/TAZ (Cell Signaling #4912 ...
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bioRxiv - Immunology 2020Quote: ... Antibodies used to detect proteins involved in glycolysis (Hk1 clone C35C4, Glut1 clone D3J3A and HIF-1a D1S7W) were from Cell Signaling (all at 1:500) were used to intracellular staining ...
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bioRxiv - Systems Biology 2022Quote: ... Immunoblot analysis was used to assess the adequacy of nuclear purification by measuring Lamin A/C (rabbit, 1:1000, Cell signaling # 2032, a nuclear protein).
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bioRxiv - Physiology 2023Quote: ... we loaded 25 µg protein of each sample to SDS-PAGE gels and incubated the membranes with 1:1000 eIF2α antibody (Cell Signaling Technology #9722, Danvers, MA) or 1:1000 Phospho-eIF2α antibody (Cell Signaling Technology #9721 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were extracted at 3 hours post-treatment and analysed by western blot following overnight incubation with antibodies against the following primary proteins: Vinculin (E1E9V, rabbit, Cell Signaling Technology, 13901, 1:1000), Phosphorylated p44/42 MAPK (ERK1/2 ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein concentration was determined by a BCA assay (Pierce) 60 μl Protein A beads with 50% slurry (Cell Signaling Technology, #9863S) were then added and incubated for 6 hours to pull down the antibody-protein complex ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting mixtures of protein-DNA and antibody were further incubated with ChIP-Grade Protein G Magnetic Beads (9006, Cell Signaling Technology). After sequential washing with low- and high-salt solutions ...
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bioRxiv - Developmental Biology 2020Quote: ... Total protein was extracted with RIPA buffer (Cell Signaling) with cOmplete protease inhibitor (Roche ...
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bioRxiv - Physiology 2019Quote: ... mTOR and the reference protein β-actin (Cell Signaling). Protein quantification was determined using fluorescence ...
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bioRxiv - Cell Biology 2022Quote: ... ChIP-Grade protein G magnetic beads (Cell Signaling, 9006S), 25 μl per IP ...
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bioRxiv - Microbiology 2020Quote: ... P-6S Ribosomal protein (Ser235/236, D57.2.2E Cell Signaling), S6 Ribosomal protein (5G10 ...
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bioRxiv - Neuroscience 2022Quote: ... PhosphoSitePlus: Protein Modification Site (Cell Signaling Technology; RRID: SCR_001837), Image J (RRID ...
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bioRxiv - Microbiology 2019Quote: ... rabbit anti-protein disulfide-isomerase (PDI, Cell Signaling C81H6) for labeling ER was used at 1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µl of Protein A agarose beads (Cell Signaling) were added and incubation continued for 3-5 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... phospho-S6 Ribosomal protein (Ser240/244, D68F8, Cell Signaling #5364 ...
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bioRxiv - Microbiology 2022Quote: ... An anti-Tyk2 IgG protein (Cell Signaling Technology, 14193) was used as a control for the VitC binding experiment ...
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bioRxiv - Cancer Biology 2023Quote: Proteins were extracted in RIPA buffer (Cell Signaling Technologies), resolved on Bolt Bis-Tris mini-gels (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Phospho-S6 Ribosomal Protein (Ser235/236; Cell Signaling 4858S), NRF2 (Cell Signaling 12721) ...
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bioRxiv - Neuroscience 2021Quote: ... The immunodetection of selected proteins was performed overnight at 4 °C with the following antibodies: p62 (Cell Signaling Technology Inc, Danvers, MA, USA, 5114, 1:1000), LC3b (Abcam ab51520 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/μl (500 μg protein) samples were prepared and incubated overnight at 4 °C in primary antibody against acetyl-lysine (Cell Signaling; Cat No. 9814 (1:100)) ...