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Citations for Cell Signaling Technology :
301 - 350 of 10000+ citations for Rat Dynamin 1 Like Protein DNM1L ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Quantified protein (between 200-500 µg) was incubated with protein-specific or isotype control antibody (Cell Signaling Technology) in lysis buffer at 4°C overnight with head over tail rotation ...
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bioRxiv - Immunology 2020Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins bound to Protein-A Dynabeads were pulled down using a magnetic separation rack (Cell Signaling, no. 14654). After 5 washes in NP-40 lysis buffer ...
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bioRxiv - Immunology 2023Quote: The protein concentration was adjusted to 30 μg by adding protein lysis buffer (Cell Signaling Technology, Danvers, MA) and 4x loading buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were collected and incubated with a 30 μl bed volume of protein-A-sepharose beads and 1.5 μl of α-IGF-1 receptor β (D23H3) XP (Cell Signaling) per sample ...
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bioRxiv - Cancer Biology 2020Quote: ... and rabbit monoclonal vinculin antibody (clone E1E9V; #13901, 1:1000; epitope: amino terminus of human vinculin protein) were purchased from Cell Signaling Technology.
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bioRxiv - Plant Biology 2021Quote: ... The phosphorylated MAPK3 and MAPK6 proteins were detected using polyclonal anti-phospho-p44/42 MAPK (Erk1/2, 1:1000; Cell Signaling Technology), and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000 ...
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bioRxiv - Biochemistry 2019Quote: ... in tris-buffered saline Tween 20 (TBS-T) for 1 h at RT and then blotted for phosphorylated proteins (Cell Signaling Technology; phospho-EGFR Y1068 #3777 ...
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bioRxiv - Neuroscience 2020Quote: ... 20 µg of total brain protein were loaded and anti-GFAP antibody (D1F4Q) XP Rabbit mAb (1:3000; Cell Signaling Technology, 12389), anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then prepared for Western blot analysis and total protein was incubated with E-Cadherin (1:1000, Cell Signaling Technology, #3195), Fibronectin (1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... blocked with 5% bovine serum albumin for 1 hour at room temperature and incubated overnight with a primary antibody against the principal ventricular GJ protein Cx43 phosphorylated at Ser368 (pCx43, 1:1000, #3511S, Cell Signaling Technologies), at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein lysates were separated by SDS-PAGE and proteins of interests detected with the following antibodies: phosphorylated (p-VASP) Ser239 (rabbit, 1:1000, Cell Signaling #3114S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Neuroscience 2023Quote: ... anti-Phospho-p70 ribosomal protein S6 kinase (Thr389) (108D2) (pS6K) (rabbit monoclonal, #9234, RRID: AB_2269803, Cell Signaling Technology; 1:1000 for WB), anti-p70 S6K α (S6K)(mouse monoclonal clone H-9 ...
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bioRxiv - Cell Biology 2024Quote: ... Specimens were incubated overnight at 4 °C with rabbit anti-SARS-CoV-2 nucleocapsid protein (Cell Signaling Technology, USA, #26369, 1:200), mouse anti-ACE2 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... cFos protein was labelled in the same manner using rabbit polyclonal antibody c-Fos (9F6) (1: 1000, Cell Signaling Technology Inc, 2250) and Alexa Fluor 488 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... For visualization of SARS-CoV-2 infected cells via detection of N protein in Calu-3 cells we used anti SARS-CoV-1/2 Nucleocapsid Mouse anti-N clone (Cell Signaling, 1C7C7) 1:1000 50uL/well.
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bioRxiv - Molecular Biology 2021Quote: Protein A magnetic beads (Cell Signaling Technology) were pre-washed with cell lysis buffer twice ...
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bioRxiv - Developmental Biology 2021Quote: ... containing a Protein A slurry (Cell Signaling). A non-specific antiserum (control IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... S6 ribosomal protein (2217; Cell Signaling Technology), Akt1 (2938 ...
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bioRxiv - Immunology 2021Quote: ... coupled to Protein G beads (Cell Signaling) overnight with rotation at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... containing a Protein A slurry (Cell Signaling). A non-specific antiserum (control IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... protein A/G agarose beads (Cell Signaling) were added and allowed to rotate for another 2h ...
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bioRxiv - Developmental Biology 2022Quote: ... rat TS cells were exposed to a phosphatidylinositol 3-kinase (PI3K) inhibitor (LY294002, 10 μM, 9901, Cell Signaling Technology), chloroquine (50 μM for 24 h ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed in 1x TBST and incubated with appropriate HRP-conjugated secondary (Goat anti-rat 7077, Cell Signaling, Goat anti-mouse 7076 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins of interest were detected with HRP-conjugated horse anti-mouse IgG antibody (1:5000, 7076S, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA) or HRP-conjugated goat anti-rabbit IgG antibody (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed on 6% acrylamide SDS-PAGE gels followed by immunoblotting with rabbit anti-myc (1:3000, 71D10, Cell Signaling Technology, 2278S). In vitro dephosphorylation experiments were performed in three biological replicates.
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bioRxiv - Neuroscience 2019Quote: ... Formalin fixed brains were paraffin embedded and analyzed using the following antibodies according to immunohistochemistry protocols described previously (Chakrabarty, 2010; Chakrabarty, 2015): Glial Fibrillary Acidic Protein (GFAP, Cell Signaling, 1:1000) and ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cancer Biology 2019Quote: ... 15 μg of extracted proteins were separated by SDS-PAGE and revealed by antibodies directed against actin (1: 1000, Cell Signaling Technology #8457), GAPDH (1 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit monoclonal anti-SorLA antibody specific to the N-terminus of human SorLA protein (1:1,000; D8D4G #79322; Cell Signaling, Danvers, MA, USA) for the detection of SorLA759 proteins ...
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bioRxiv - Microbiology 2021Quote: ... was used for western blotting at a dilution of 1:1,000 and rabbit polyclonal antiserum reactive against protein kinase A (PKA) substrates (Cell Signaling Technology, Danvers, MA) was used for western blotting at a dilution of 1:1,000 ...
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bioRxiv - Genomics 2023Quote: ... Correct expression of the mAID-Dam-Lamin B1 fusion protein as well as the wildtype Lamin B1 protein was confirmed using Immunofluorescence staining using an anti-HA antibody at 1 in 500 dilution (Cell Signaling Technologies, C29F4) and an anti-LaminB1 antibody at 1 in 500 dilution (Abcam ...
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bioRxiv - Biophysics 2023Quote: ... mAb #3288) and Lysosome Associated Membrane Protein 1 (LAMP1) specific rabbit monoclonal antibody (cat. no. mAb #9091) were purchased from Cell Signaling Technology (USA). Anti-caveolin-1 rabbit polyclonal antibody was acquired from BD Biosciences (USA ...
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bioRxiv - Immunology 2020Quote: ... The same amount of protein was immunoprecipitated with anti-NEMO and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). The IKKα/β kinase activity in the IP complex was tested by using IKKβ Kinase Enzyme Kit (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... that combines the IgG binding domain of both Protein A and Protein G were used to cross-link anti-Kv11.1 antibodies (Cell Signaling). Stock beads with a concentration of 10 mg/ml were washed four times with 10 volumes of Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Bioengineering 2020Quote: ... followed by incubation with 1:2,500 dilution of primary antibodies for CD68 (rat anti-mouse, ABD SeroTec) and cleaved Casp3 (rabbit anti-mouse, Cell Signaling) in 0.5% w/v BSA in 1X PBS for 24 h at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... isolated rat cardiomyocytes were lysed in RIPA buffer (Cayman #10010263) supplemented with protease and phosphatase Inhibitor cocktail (Cell Signaling #5872S) on ice for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: Protein A agarose beads (Cell Signaling Technologies 9863S) were washed and incubated in NET150 buffer (20 mM Tris HCl ph ...
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bioRxiv - Cell Biology 2022Quote: ... ribosomal protein S6 (#2217 CST) from Cell Signaling. Mouse monoclonal anti-beta Actin AC-15 HRP (#AB49900 ...
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bioRxiv - Molecular Biology 2021Quote: ... or directed to T6B-fusion protein (Cell Signaling anti-FLAG #8146S ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μl Protein G agarose beads (Cell Signaling) were washed in IP buffer and blocked with bovine serum albumin (BSA) ...
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bioRxiv - Biochemistry 2019Quote: ... When blotting for total proteins (Cell Signaling Technology; EGFR #4267 ...
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bioRxiv - Cell Biology 2020Quote: ... or Protein A HRP conjugate (Cell Signaling Technology) diluted at 1:5000 in blocking buffer for 45 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... S6 ribosomal protein mouse mAB (2317S, Cell Signaling), phospho-p70 S6 kinase (Thr389 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A/G agarose beads (Cell Signaling Technology) were then added and incubated for 2 hr at 4°C ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween and detection of total CHK1 or CHK2 proteins was carried out for 1 h at RT using mouse-anti-Chk1 mAb (2G1D5) (Cell Signaling 2360; Lot 3) or mouse anti-Chk2 mouse mAb (BD Biosciences 611570) ...
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bioRxiv - Neuroscience 2019Quote: ... anti-Microtubule-associated proteins 1A/1B light chain 3B (LC3B-I/-II) at 1:1000 dilution (14, 16 kDa, Cell Signaling Technology, Danvers, MA); anti-monocarboxylate transporter 1 (MCT1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...