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Citations for Cell Signaling Technology :
301 - 350 of 10000+ citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and blots (20-50 µg of total protein) were probed with the following antibodies: HA (3724S, Cell Signaling; 1:1500), BRCA1 (SC6954 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µl SureBeads were washed in 0.1% TBST for 3 times and then incubated with 1:50 ACSS2 primary antibody (Rabbit, Cell Signaling) or 1:50 IgG control (Cell Signaling Technology ...
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bioRxiv - Genetics 2020Quote: ... Larval carcasses were stained with Rabbit anti-Cleaved Caspase-3 (Cas-3) at 1:100 (Cell Signaling). Secondary antibodies were purchased from Jackson Immunoresearch ...
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bioRxiv - Biophysics 2021Quote: ... and phospho-myosin (rabbit phospho-myosin light chain 2 (Thr18/Ser19) antibody (dilution 1:50 in PBS 0, 5% BSA, #3674, Cell Signaling) for 2 h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... rinsed in phosphate buffer 0.2 % Triton for 15 min and incubated with rabbit anti-P-cad (1:50; Cell Signaling; 2130S) or anti-pSrc (pY419 ...
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bioRxiv - Neuroscience 2020Quote: ... Signaling pathway inhibitors were added to cells 1 h prior to illumination at the following concentrations: 50 μM LY294002 (PI3K inhibitor, Cell Signaling) or 30 μM PD98059 (MEK1 inhibitor ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibody solution mix contains two primary antibodies raised in two different species (mouse and rabbit) targeting the proteins of interest: rabbit anti-mitofusin 2 (1:50, mAb D2D10, Cell Signaling), rabbit anti-VDAC (1:200 ...
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bioRxiv - Immunology 2022Quote: Four μm TMA sections were stained with PTEN Rabbit mAb (1/50 dilution; clone D4.3 XP; Cell Signaling Technologies, MA, USA). Multiplex immunofluorescence staining of TMA sections was performed using antibodies specific to CD8 ...
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bioRxiv - Immunology 2022Quote: ... permeabilised cells were stained with PE-conjugated anti-p-Syk (clone C87C1; 1:50 dilution; Cell Signaling Technology, Danvers, MA, USA), anti-p-S6 (clone D57.2.2E ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... and the beads were resuspended in 50 μl of antibody binding buffer (wash buffer + 2 mM EDTA + 0.025% digitonin) containing an antibody against H3K27me3 (Cell Signaling Technologies, 1:100 dilution of 1 mg/ml). IgG (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... chloroquine at 50 μM (#14774 from Cell Signaling). In all three cases ...
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bioRxiv - Cell Biology 2024Quote: ... anti-XBP1-s (:50, 40435S, Cell Signaling Technology), anti-GRP78 (1:20 ...
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bioRxiv - Cell Biology 2022Quote: ... Cas pY410 (Cell Signaling Technology), Erk1/2 (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... permeabilised with methanol/acetone (50/50) and then stained intracellularly by incubating cells with rabbit antibody against p-(Y694) STAT-5 (D47E7) XP (Cell Signaling), mouse antibody against BCL-2 (100 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei pellets were washed twice with 200 μL of Dig-wash buffer before resuspending in 100 μL Dig-wash buffer supplemented with 2 mM EDTA and a 1:50 dilution of H3K27ac primary antibody (Cell Signaling, 8173) and incubated overnight at 4°C on an overhead rotator ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... They were rinsed with 1x PBS again and incubated with 50 μL of blocking solution (1x PBS, 0.5% goat serum) with 1:300 anti-rabbit mTOR (Cell Signaling Technology, #2983S) for 1 h at RT ...
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bioRxiv - Immunology 2020Quote: ... Following antibodies that were diluted in blocking buffer were used to detect early endosomes and lysosomes at 4C’ overnight: rabbit anti-EEA1 (Clone: C45B10, 3288S, 1:50, Cell Signaling technology), rat anti-LAMP1 (Clone ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and stained with 1:50 RBD-sfGFP expression medium (prepared as previously described 22 and 1:250 anti-myc Alexa 647 (clone 9B11, Cell Signaling Technology) in PBS-BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... The neutrophils were then incubated overnight at 4°C with primary antibodies: anti-histone H3 (1:50 dilution; Cell Signaling Technology, 3680) and either anti-NE (1:100 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... and two different YAP1 antibodies were used to characterize YAP1 expression patterns: mouse monoclonal anti-YAP1 antibody (catalog # sc-101199,1:50) and rabbit monoclonal anti-YAP1 antibody (catalog # 14074S, Cell Signaling,1:100). To prevent nonspecific binding of the antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Digestion was stopped by adding 1× stop buffer (digitonin solution, RNase A, and 50 pg S. cerevisiae spike-in DNA (Cell Signaling Technology) for normalization ...
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bioRxiv - Microbiology 2022Quote: ... Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T). The negative control sample used ΔKu80 parasites (the parental line ...
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bioRxiv - Cell Biology 2024Quote: ... the membrane was blocked in Tris-buffered saline (50 mM Tris with 150 mM NaCl) with 0.05% tween-20) containing 1% nonfat dry milk (Cell Signaling Technology 9999S) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... and the appropriate primary antibodies were added at 1:50 dilution ratio (Rabbit anti-H3K23ac, Invitorgen; Rabbit anti-H3K27ac, Cell Signaling Technology; CUT&TAG IgG control ...
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bioRxiv - Cell Biology 2024Quote: ... The precleared lysate was reserved and incubated with 10 µL of a 1:50 dilution of rabbit anti-HA antibody (C29F4, Cell Signaling Technology) for 2-hr at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto, CA, USA] and anti-Gapdh [1:5000; Cell Signaling, Davers, MA, USA; 14C10]).
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bioRxiv - Cancer Biology 2022Quote: Immunofluorescence was performed using the following specific antibodies: phospho-p130-Cas (Y410) (Cell Signaling Technology, Cat. #: 4011, 1:200), phospho-FAK (Y397 ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 nM Calyculin A (CalA; Cell Signaling Technology (CST), 9902S ...
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bioRxiv - Cancer Biology 2022Quote: ... Prdx1 (Cell Signaling Technologies, D5G12, Cat# 50-191-580), HSP90 (Cell Signaling Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Rabbit anti-H3K4me3 (Cell Signaling Technology 9751; RRID:AB_2616028,1:50), or rabbit IgG or Sheep IgG for each reaction ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... anti-Rab11 (1:50; CST, C45B10, #3288) or anti-LAMP1 (1:100; CST, D2D11 XP, #9091) rabbit monoclonal antibodies were purchased from Cell Signaling Technology (CST). Alexa 488 goat anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained for 1hr in permeabilization buffer with Myc antibody (Myc/n-Myc D3N8F Rabbit mAb PE Antibody, #35876, Cell Signaling, 1:50). Myc expression (expressed as geometric MFI ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used at the indicated dilutions: rabbit polyclonal anti-phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) at 1:50 (Cell Signaling Technology, #3141), rabbit anti-Msn M1 at 1:750 (gift from Thomas Moss ...
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bioRxiv - Immunology 2024Quote: ... Cells bound to the beads were resuspended in Dig-Wash Buffer and incubated with a 1:50 dilution of primary antibodies (rabbit anti-H3K4me3, Active Motif,39016; rabbit anti-CXXC1, abcam, ab198977; normal IgG, Cell Signaling, 2729) at 4 ℃ overnight ...
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bioRxiv - Systems Biology 2024Quote: ... 1% nuclease-free BSA) and a 1:50 dilution of the appropriate primary antibody (anti-Eomes, Abcam, ab23345 and rabbit IgG, Cell Signaling Technology, 66362). Primary antibody incubation was performed on a nutator platform overnight at 4°C ...
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bioRxiv - Pathology 2020Quote: ... and phospho-βcat (pS33-βcat 1:200, Santa Cruz, Santa Cruz, CA # SC-16743-R; pS33/37/T41-βcat 1:1200, Cell Signaling Technology, Danvers, MA, #9561) were used ...
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bioRxiv - Microbiology 2021Quote: ... The antibodies used in the WB assay included anti-PDGFRβ rabbit mAb (1:1000, Cell Signaling Technology, Danvers, CA, USA), anti-JEV NS3 rabbit antiserum (kindly provided by C.J ...
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bioRxiv - Biochemistry 2020Quote: ... and the endoplasmic reticulum (ER) was labeled with a rabbit anti-Calnexin antibody (1:1000, Cell Signaling Technology, CA, USA). The Golgi apparatus was labeled with a rabbit anti-GM130 antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: Cryosections of P0 mouse brains were immunolabeled with cleaved-caspase 3 (c-cas 3) antibody (Cell Signaling #9961, 1:4000) for apoptosis analysis ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were then precleared by incubation for 1 hour at 4°C while rotating with 50 μL protein A agarose beads (Cell Signaling Technologies, Cat #9863) CCNT1 antibody (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The MEL cells were bound to Concanavalin A Beads and Incubated with 1:50 rabbit polyclonal Phospho-Rpb1 CTD Ser5 and Ser2 antibody (Cell Signaling 13523 and 13499). Guinea pig a-rabbit antibody was used at 1:100 dilution as secondary antibody ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates containing the same amount of protein from EGF-stimulated cells were mixed with EGFR monoclonal antibody (Cell Signaling #4267, 1:50 dilution) and incubated overnight at 4°C using end-to-end rotation ...
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bioRxiv - Cancer Biology 2024Quote: ... and the appropriate primary antibodies were added at 1:50 dilution ratio (Rabbit anti-H3K23ac, Invitorgen; Rabbit anti-H3K27ac, Cell Signaling Technology; CUT&TAG IgG control, Cell Signaling Technology). Primary antibody incubation was performed on a rotating platform overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... the samples were washed twice with incubation buffer (0.5% BSA in PBS) and stained with an anti-cleaved caspase-3 Alexa Fluor® 488 antibody (Cell Signaling, 9603, 1:50) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: Mouse tumor samples were prepared and subjected to immunohistochemistry based on the procedure described in our previous study with modifications as follows.(30) The primary antibody used for CD8 (Cell Signaling, #98941, 1:50) was diluted in a blocking solution and incubated at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times with PBS-T (PBS with 0.05% Tween-20) with automatic plate washer (250 μL per cycle) and 50 μL of HRP anti-mouse IgG (Cell Signaling Technology #7076, 1:3,000) or HRP anti-mouse IgA (Southern Biotech #1040-05 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 30min in the primary + secondary antibody solution: 2%BSA-PBS + Rabbit anti-mouse Phosopho-Akt (Ser473) antibody (Cell Signaling Technologies 4060; 1:50), Goat anti-Rabbit Alexa 488 secondary antibody (ThermoFisher A32731 ...
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bioRxiv - Neuroscience 2024Quote: ... The following secondary antibodies were used: Anti-Mouse IgG (H+L) Fab2 Alexa Fluor 488 Conjugate (Cell Signaling Technologies, Danvers MA, 4408S, dilution 1:50), Anti-Rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... F4/80 (Cell Signaling, Ca. No 70076) diluted 1:200 with 36 minutes of incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of protein A magnetic bead slurry (73778S, Cell Signaling) were aliquoted per sample and washed with 500 μL of Complete RIPA buffer ...