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Citations for Cell Signaling Technology :
3201 - 3250 of 10000+ citations for Anti PAK3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The membranes were incubated overnight with a rabbit anti-FosB antibody (2251; 1:500 dilution; Cell Signaling Technology), which recognizes both FOSB and ΔFOSB ...
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bioRxiv - Physiology 2024Quote: ... Cleaved-Casapase-3 staining was performed using anti-Cleaved-Casapase-3 antibody (1:200, Cell Signaling Technologies, MA) and secondary Alexa Fluor 488 anti-rabbit antibody (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated for 48 hr at 4C in 1× PBS containing the primary antibodies (Chicken anti-GFP, AbCam cat. No. #13970. 1:2000; Rabbit anti-NeuN, Cell Signaling Technology, cat. No. #24307, 1:1000). Slices were then washed 3 × 5 minutes in 1x PBS and incubated in 1x PBS containing the secondary antibodies for 2 hours (Alexa Fluor 488 ...
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... Then membranes were incubated for 1 h at RT with secondary anti-mouse or anti-rabbit HRP-linked antibodies (Cell Signaling Technology #7076 or # 7074; 1:5000) diluted in Bullet Blocking One and were washed three times for 15 min in 1×TBST buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were blotted on Nitrocellulose membrane (Cytiva) and revealed using following antibodies: mouse anti-AGR2 (Santa Cruz Biotechnology, Clone 6C5, 1/1000, rabbit anti-XBP1s (Cell Signaling Technologies, clone E9V3E, 1/1000), rabbit β-Tubulin-HRP (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were blocked in 5% evaporated milk in Tris-based saline with Tween 20 (0.05% TBST) for 1 hour and followed by incubating with primary antibodies overnight at 4 °C (Anti-HA: Cell Signaling Technology, 3724S; anti-GAPDH: Thermo, MA5-15738). After three rinses with TBST ...
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bioRxiv - Biochemistry 2022Quote: ... Primary antibodies (rabbit insulin antibody: #4590 Cell Signaling Technology, OC (generic amyloid antibody) and rabbit β-actin antibody ...
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bioRxiv - Plant Biology 2023Quote: ... Then 4 µg of primary antibodies (αHA antibody: Biolegend, #901502; αH2A.Z antibody: abcam, ab4174; αH2AK121ub antibody: Cell Signaling Technology, 8240S ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times with TBS-T (0.05% v/v Tween-20 in TBS) before incubation with HRP conjugated antibody (Cell Signaling Technology 7076S; anti-mouse-HRP or Cell Signaling Technology; 7074S; anti-rabbit-HRP) at 1:1000 in 2.5% (w/v ...
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bioRxiv - Biochemistry 2019Quote: ... Membranes were then incubated with the appropriate secondary antibodies in TBS-T for 30 min at RT at 1:4000 dilution (Cell Signaling Technology, Anti-rabbit #7074, Anti-mouse #7076). Following washes with TBS-T ...
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bioRxiv - Cell Biology 2019Quote: ... anti-phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204) rabbit antibody (no. 9101, Cell Signaling Technology, 1:2,000 dilution); anti-α-Actin rabbit antibody (no ...
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bioRxiv - Cell Biology 2020Quote: ... The fluorescent secondary antibodies were conjugated with either Alexa Fluor 594 (1:1,000, anti-rabbit, Cell Signaling Technology, 8889) or Alexa Fluor 647 (1:1,000 ...
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bioRxiv - Genetics 2019Quote: ... as described previously.21 The following antibodies were used for western blotting: anti-S6 ribosomal protein (Cell Signaling Technology), anti-phospho-S6 ribosomal protein (Ser240/244 ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... Western Blots were performed with the following primary antibodies: rabbit-anti-p-ERK1/2 (1:500, Cell Signaling 9101), rabbit-anti-Erk1/2 (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated with the appropriate HRP-conjugated secondary antibody (1:000, anti-mouse IgG, Cell Signaling Technologies, 7076P2 ...
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bioRxiv - Immunology 2019Quote: ... The following primary antibodies were used to probe protein levels by immunoblotting: anti-GAPDH (Cell Signaling Technology, clone D16H11), anti-β-Actin (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2020Quote: ... protein from transfected 293T cells was analyzed by western blotting with an anti-GFP antibody (clone D5.1, Cell Signaling). This corresponded with an increase in protein size from 27 to 32 kDa by western blotting (Fig S2C) ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by incubation with anti-rabbit IgG HRP-linked secondary antibody (1:5000, 7074S, Cell Signaling, Danvers, MA, USA). TGFBR1 was detected with rat anti-mouse TGFBR1 primary antibody (1:300 ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were then incubated with the appropriate HRP-conjugated secondary antibody (1:000, anti-mouse IgG, Cell Signaling Technologies, 7076P2 ...
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bioRxiv - Microbiology 2019Quote: ... 1% bovine serum albumin in PBS) and anti-phospho-Ser172-TBK1 (D52C2) XP Rabbit monoclonal antibody (Cell Signaling #5483) for three hours at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... samples were blocked with 2% BSA and incubated with primary antibodies including Rabbit anti-ALK (1:200, Cell Signaling), Rabbit anti-MYCN (1:50 ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated with a 1:1000 dilution of anti-β-Actin antibody (#4970, Cell Signaling Technology, Cambridge, UK). Following incubation ...
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bioRxiv - Neuroscience 2021Quote: The following primary antibodies were used: anti-β-Catenin (rabbit polyclonal, 1:200, Cell signaling, Cat# 9587, RRID: AB_10695312); anti-cleaved caspase 3 (rabbit polyclonal ...
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bioRxiv - Neuroscience 2020Quote: ... and probed overnight with the following primary antibodies: rabbit anti-phospho-(Ser) PKC substrates (1:15,000; Cell Signaling #2261), mouse anti-pan PKC (1:10,000 ...
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bioRxiv - Cancer Biology 2021Quote: ... A rabbit monoclonal anti-NDRG1 antibody was at a dilution of 1:1,000 (Cell Signaling Technology, Danvers, MA, USA). DNMT1 ...
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bioRxiv - Cancer Biology 2020Quote: ... as well as anti-rabbit HRP-linked (catalog no. 7074) secondary antibodies were all obtained from Cell Signaling Technology. Bands were detected with a MicroChemi chemiluminescent system (DNR Bio-Imaging Systems ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used for western blotting: Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19) (3674, Cell Signaling Technology), rabbit anti-Myosin Light Chain 2 (3672 ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody used for immunofluorescence include anti-phospho-p44/p42 ERK1/2 (Thr202/Tyr204) (Cat# 4370) from Cell signaling technologies used at 1:200 dilution ...
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bioRxiv - Systems Biology 2020Quote: ... followed by incubation with a 1:2,500 dilution of HRP-conjugated horse anti-mouse IgG antibody (Cell Signaling #7076S). All antibody incubations were performed in 1 % w/v non-fat milk blocking in 1X TBS ...
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bioRxiv - Plant Biology 2021Quote: ... The activated MAP kinases were detected using anti-p42/44 MAPK primary antibody (1:2500, Cell Signaling Technology, 4370) overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were added to the samples for overnight incubation (rabbit anti-phospho-Histone H3 (1:200; Cell Signaling); mouse anti-HA (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Phosphorylation of EphA2 (Ser 897) was detected with a phosphospecific anti-EphA2 antibody (Cell Signaling Technology; #6347, clone D9A1) and total EphA2 was detected with an anti-EphA2 antibody (Cell Signaling Technology ...
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bioRxiv - Systems Biology 2020Quote: ... then incubated with anti-vimentin antibody (clone D21H3, Cell Signaling Technologies, (1:500 dilution in 5% milk/TBS-T) and with anti-GAPDH antibody (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at 4 °C overnight with the following antibodies: anti-PKR (1:1000; Cell Signaling cat. No. 3072), anti-p-PKR (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... blots were probed with primary antibodies at 4°C overnight: rabbit anti-phospho-eIF2αS51 (Cell Signaling, #9721, 1:1000), rabbit anti-eIF2α(Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... MIN6 cells were incubated with primary antibody rabbit monoclonal anti-NKX6.1 (cat. 54551, Cell Signaling Technology, Danvers, MA, USA) diluted 1:100 in BSA 1% in PBS for 1h ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with 20 μl of rabbit anti-p44/42 primary antibody (Cell Signaling Technology, Danvers, MA, 9101), prepared in blocking buffer at 1:20 dilution for 12 h at 24°C in a humid chamber with gentle shaking ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated for 60 min with the following primary antibodies: anti-YAP (1:100; 14074, Cell Signaling Technology), anti-phosphoPaxillin (1:400 ...
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bioRxiv - Cell Biology 2022Quote: ... and the supernatant containing fragmented chromatin was incubated with 2 μl of anti-YY1 antibody (D5D92Z, Cell Signaling, 46395) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cleaved caspase-3 was analyzed using a rabbit anti-cleaved caspase-3 monoclonal antibody (1:500, Cell Signaling Technology) followed by a second staining with FITC-anti-rabbit IgG (1:200 ...
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bioRxiv - Immunology 2020Quote: ... then washed and stained 1 hour with Alexa Fluor 647-conjugated anti-rabbit secondary antibody (Cell Signaling Technology #4414). For phospho-Stat3 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells overexpressing Rab35 were detected using an anti-HA primary antibody (data not shown; Rabbit, Cell Signaling; Mouse, Biolegend) + Alexa Fluor-405 secondary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were used at 1:2000 dilution in blocking buffer: Anti-rabbit IgG HRP-linked (7074S, Cell Signaling) and anti-mouse IgG HRP-linked (7076 ...
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bioRxiv - Neuroscience 2021Quote: ... and subsequently incubated with primary antibodies overnight at 4°C (anti-PKD1 (1:1000, Cell Signaling Technology #2052, RRID:AB_2172539), anti-pS916PKD (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Phosphorylated SakA (P- SakA) was detected using a rabbit anti-P-p38 antibody #9211 (Cell Signaling Technology, Danvers MA) at 1:1,000 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... Full loss of PTEN expression was finally validated by Western blotting (anti-PTEN antibody, Cell Signaling, 9559S, 1:1000). sgRNAs come from Cederquist et al.57:
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bioRxiv - Biochemistry 2022Quote: ... The blot was probed using anti-Mouse IgG secondary antibody linked with horse radish peroxidase (Cell Signaling Technology, #7076) (1:3000 ...
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bioRxiv - Plant Biology 2019Quote: ... Phosphorylated (activated) MAPK proteins were detected using anti-phospho-p44/42 MAPK T202/Y204 (pERK) antibodies (Cell Signaling; 9101). SlM3Kα proteins were detected using of anti-HA antibodies (Krackeler ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibodies to Notch4 (2423) and Nucleolin (14574) and anti-rabbit Alexa 543 were from Cell Signaling Technology (MA, USA). All other reagents were purchased from Invitrogen (CA ...
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bioRxiv - Developmental Biology 2019Quote: ... then incubated with appropriate primary antibodies overnight at 4°C: anti-cleaved NOTCH1 (Cell Signaling Technologies, 4147, 1:200), anti-NOTCH1 (Origene ...