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Citations for Cell Signaling Technology :
251 - 300 of 695 citations for Serum Zinc Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... and autophagy detection kit (Cell Signaling). ChIP was performed as described.39 Antibodies used were Blimp-1(R21),38 ...
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bioRxiv - Cell Biology 2020Quote: ... c-Kit (Cell Signaling Technology, 3074S). F-actin was stained with phalloidin (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Supershift assays were performed by incubating monoclonal antibody (Ab) to specific AP-1 transcription factors c-Jun (60a8, Cell Signaling), c-Fos (9F6) ...
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bioRxiv - Cell Biology 2022Quote: ... adipocytes were treated with assay medium +/-insulin for 10 minutes and harvested in cell lysis buffer (Cell Signaling Technology #9803) with phosphatase inhibitor (Thermo Fisher Scientific 78442) ...
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bioRxiv - Bioengineering 2020Quote: ... BrdU was added to the cells and proliferation was assessed 12h later using a colorimetric BrdU assay (Cell Signaling, Inc.).
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bioRxiv - Microbiology 2021Quote: Kinase assay was performed using recombinant proteins purified from E.coli and commercially available 10X Kinase buffer (Cell Signaling Technology, #9802). Recombinant proteins (N’-His PINK1 and N’-His Ajuba ...
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bioRxiv - Microbiology 2021Quote: ... The antibodies used in the WB assay included anti-PDGFRβ rabbit mAb (1:1000, Cell Signaling Technology, Danvers, CA, USA), anti-JEV NS3 rabbit antiserum (kindly provided by C.J ...
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bioRxiv - Immunology 2020Quote: Cells were collected and lysed on ice for 30 min in 1X radioimmunoprecipitation assay (RIPA) buffer plus (Cell Signaling Technology) with protease and phosphatase inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: Cell lysis was performed using Radioimmunoprecipitation (RIP) assay buffer (#9806, Cell Signaling Technology, 3 Trask Lane, Danvers, MA 01923, USA) supplemented with protease and phosphatase inhibitor (#5872) ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... These cells were then subjected to ChIP assays with primary antibody against ESRRA (10 μl/IP, Cell Signaling Technology #13826) or normal rabbit IgG ...
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bioRxiv - Developmental Biology 2024Quote: The following antibodies were used in the immunostaining assays: Alexa Fluor® 488 Phalloidin (Cat# 8878, Cell Signaling, 1:200), anti-Myosin (Cat# A4.1025 ...
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bioRxiv - Cell Biology 2020Quote: ... the myc (2278) and HA epitopes (3724) and HRP-coupled anti-rabbit secondary antibody as well as Normal Donkey Serum from Cell Signaling Technology (CST); the FLAG epitope from CST (2368 ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed with Bond Wash solution 2x 10s and blocked with a phosphate-buffered saline (PBS) solution containing 5% normal goat serum (5425S, Cell Signaling, Danvers, MA) and 0.5% TritonX-100 (T8787 ...
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bioRxiv - Neuroscience 2023Quote: ... with 10% horse serum, 1% penicillin-streptomycin (gibco, Cat# 15140-122) and 0.1 ug/mL nerve growth factor (NGF, Cell signaling Technologies, Cat# 84087S). An additional 2-3 mL of culture media was added ...
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bioRxiv - Genetics 2022Quote: ... Neurons were subsequently blocked with 5% normal goat serum in 1x PBS for 1 h and then incubated with primary antibodies as follows: anti-GFP (Cell signaling, #2555S, R), anti-Gephyrin (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... containing 5% bovine serum albumin (BSA) for 30 min and then incubated with primary antibodies against p-ERK (#4370S, 1:1000, Cell Signaling, Danvers, MA), p-P38 (#4511S ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with TBS containing 2% (m/v) bovine serum albumin (BSA) and then incubated with antibodies raised against the HA epitope (Cell Signaling Technology, USA) diluted 1:1000 in the same solution ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were blocked with TBST/5% Normal Goat Serum for 1 hour at RT before overnight incubation with primary antibody diluted in Diluent CST (Cell Signaling Technology, 8112) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were washed and then blocked using 1% Goat serum in TBS for one hour at room temperature before adding primary antibodies to detect Cd45 (Cell Signaling, 1:500) and Cd11b (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... permeabilized cells were blocked for 30 minutes at room temperature (RT) in 5% (v/v) normal goat serum (NGS) (Cell Signaling Technology, #5425) DPBS solution ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were stained for β-galactosidase using the activity kit (Kit 9860S) from Cell Signaling Technologies according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The pull-down assay of ZFP982-MYC tagged protein was performed through co-Immunoprecipitation (co-IP) using MYC-Tag (9B11) antibody (#2276, Cell Signaling). For MYC-co-IP ...
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bioRxiv - Immunology 2021Quote: ... anti-β-actin (3700) and the secondary antibodies used for western blot and immunofluorescence assay were purchased from Cell Signaling Technology. Anti-SYVN1 (ab170901 ...
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bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... For immunoprecipitation assays, cell lysates were incubated with Protein G Agarose (cat # 20398, Pierce) and anti-Flag antibody (cat # 2368, Cell Signaling) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... were used in immunofluorescence staining and Western blot assays: Ki-67 (AB_2797703) (mouse mAb, Cell Signaling Technology, Cat# 9449, 1:800), p-S6S235/S236 (AB_10695457 ...
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bioRxiv - Immunology 2024Quote: ... In vitro ADC cytotoxicity against these cell lines was assessed using a 72-hour XTT viability assay per the manufacturer’s recommendations (Cell Signaling Technology) and as described12.
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bioRxiv - Synthetic Biology 2024Quote: Antibodies used for live cell flow cytometry assays include Alexa Fluor 647-Anti-Myc tag conjugated antibody (Cell Signaling Technologies #2233S) used at 1:100 ...
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bioRxiv - Genomics 2020Quote: ... sections were blocked in 3% bovine serum albumin (BSA) and incubated over-night with antibodies recognizing Birc5 (#2808, Cell Signaling Technology, Danvers, MA, USA), Cyclin D1 (#2978 ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were blocked in 5% bovine serum albumin (BSA) and incubated overnight at 4 °C with primary anti-phospho-SYK (Cell Signaling Technologies, 1:1000) or anti-SYK (Cell Signaling Technologies ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked with 5% bovine serum albumin (BSA) in PBS and incubated with anti-APRT antibody or an anti-β- Actin antibody (Cell Signaling Technology, USA) at 4°C overnight followed by the corresponding secondary antibody for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... penicillin/streptomycin and 2% Fetal bovine serum (for vehicle group) or the same media with 100ng/ml recombinant colony-stimulating factor 1 (Csf1, Cell Signaling, Boston, MA, USA). Csf1 stock was diluted in saline.
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bioRxiv - Physiology 2023Quote: ... Neurons were then incubated overnight at 4°C in 1% bovine serum albumin in PBS with pCREB (Ser133) (87G3) Rabbit mAb (1:333, Cell Signaling, Cat No. #9198). Neurons were then thrice rinsed with PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Then were processed with commercial SA-β-galactosidase staining kits following provider instructions (Kit #9860 from Cell signaling).
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bioRxiv - Cancer Biology 2022Quote: ... EMT kit (Cell Signaling Technology, 1:2000), beta-actin (Santa Cruz Biotech,1:4000) ...
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bioRxiv - Immunology 2023Quote: ... with the SimpleChIP Plus kit (Cell Signaling). Sequencing libraries were prepared from isolated plasmids as for MPRA and sequencing on a MiSeq (50bp ...
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bioRxiv - Immunology 2023Quote: ... with the SimpleChIP Plus kit (Cell Signaling). The ratio of rs2836882 alleles in the PU.1-bound DNA was quantified in duplicate by TaqMan genotyping (assay C 2601507_20) ...
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bioRxiv - Immunology 2024Quote: ... using an immunofluorescence kit (Cell Signaling Technologies). Samples were stained with DAPI (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibody in 10 % Donkey Serum in PBS overnight at 4 °C: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb (Cell Signaling Technology, Cat# 5364; RRID:AB_10694233). After HSCs were washed three times with PBS + 0.1 % Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was incubated for 1 h with 5% bovine serum albumin in 1 x TBS/0.1% Tween (TBST) before being incubated with primary antibodies: pAkt (1:2000, Cell Signaling Technology, Cat. #4060, RRID: AB_2315049), and pS6RP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were blocked with 10% goat serum plus 1% BSA at room temperature for 30 minutes and then incubated with the primary antibody against Ki67 (Cell signaling, Cat #12202S, 1:800) or c-Myc (Cell signaling ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was quenched by 3% hydrogen peroxidase prior to 4-hour incubation at 4°C with 5% normal goat serum (Cell Signaling Technology®, Danvers, MA) and rabbit anti-DSG1 (clone EPR677766(B) ...
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bioRxiv - Molecular Biology 2019Quote: 500 μg of unstimulated HT29 lysate was immunoprecipitated onto Protein A/G coated wells (Pierce #15138) following the kinase assay protocol but with 0.5 μg of IKKα (Cell Signaling Technology #2682) or 0.5 μg of naïve rabbit IgG (Jackson Immunoresearch #011-000-002) ...
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bioRxiv - Immunology 2021Quote: ... Western blotting was used as the output assay to determine levels of the ribosomal protein S6 kinase (p-S6K) using an S6K and p-S6K antibodies (Cell Signaling Technology). To assess the impact of mTOR1 inhibition rapamycin (20ng/mL ...
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of puromycin for 48 hours and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of neomycin for 48 hours and then used for HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure MyD88 and TRIF expression using a rabbit monoclonal antibody to MyD88 (Cell Signaling Technology), a rabbit polyclonal antibody to TRIF (Cell Signaling Technology) ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein concentration was determined by a BCA assay (Pierce) 60 μl Protein A beads with 50% slurry (Cell Signaling Technology, #9863S) were then added and incubated for 6 hours to pull down the antibody-protein complex ...
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bioRxiv - Immunology 2022Quote: ChIP assays for histone modifications were performed as described previously (Aoyama et al., 2018) using an anti-H2AK119ub1 (#8240S; Cell Signaling Technology) and an anti-H3K27me3 (#07-449 ...