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Citations for Cell Signaling Technology :
2801 - 2850 of 8735 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were captured by addition of HIF-1α (D1S7W) XP® Rabbit mAb (Cell Signaling Technology) and incubated overnight at 4°C with rotation followed by immunoprecipitation by addition of Protein A magnetic beads and incubation for 20 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... was utilized as cytoplasmic protein loading control while anti-Lamin A/C (Cell Signaling Technologies, #4777) was used as nuclear protein loading control.
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bioRxiv - Biochemistry 2024Quote: Protein lysates were collected from cells transfected with individual reporters (cell lysis solution, Cell Signaling Technology), resolved on a 4–12% Bis-Tris gel (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: Proteins were lysed using a Cell Lysis buffer (Cat. 9803, Cell Signaling Technology, Danver, MA, USA) with PMSF (protease inhibitor ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 30min in the primary + secondary antibody solution: 2%BSA-PBS + Rabbit anti-mouse Phosopho-Akt (Ser473) antibody (Cell Signaling Technologies 4060; 1:50), Goat anti-Rabbit Alexa 488 secondary antibody (ThermoFisher A32731 ...
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bioRxiv - Cancer Biology 2020Quote: ... The primary antibody and secondary antibodies detailed information list below: PD-L1 [E1L3N®] XP ® Rabbit mAb (#13684, Cell Signaling Technology, Beverly, MA, USA), GAPDH (6C5 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... every sixth section of the LPB was incubated in a primary antibody solution containing rabbit anti HA-Tag (C29F4) monoclonal antibody (1:1000; 3724s, Cell Signaling Technologies, Danvers, MA, USA) overnight followed by incubation in a solution containing a secondary Alexa-Fluor Plus 488 donkey anti-rabbit antibody (1:3000 ...
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bioRxiv - Immunology 2020Quote: ... The lysates were then resolved by SDS-PAGE and immunoblotted with indicated antibodies (Wapl and V5-tag antibodies were purchased from Thermo Fisher Scientific #PA5-38024 and #R960-25, and β-actin antibody was purchased from Cell Signaling Technology #3700).
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bioRxiv - Cancer Biology 2023Quote: ... The HRP-conjugated secondary antibodies (anti-rabbit and anti-mouse antibodies, respectively ref 7074 and 7076) were supplied by Cell Signaling Technologies (Danvers, Massachussets, USA).
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Monoclonal antibodies against AKT (2920S) and polyclonal antibodies against phospho-AKT (Ser473) (p-AKT [Ser473]; 9271S) were purchased from Cell Signaling Technology (Boston, MA, USA). Polyclonal antibodies against extracellular signal-regulated kinase 1/2 (ERK1/2 ...
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bioRxiv - Cancer Biology 2023Quote: ... were used as primary antibodies and were detected with anti-rabbit or anti mous IgG HRP-conjugated secondary antibodies (Cell Signaling, ref #7074 and #7076). Proteins were detected by chemiluminescence using Immobilon Forte Western HRP substrate (Merk Rahway ...
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bioRxiv - Cell Biology 2023Quote: ... The HRP-conjugated secondary antibodies (anti-rabbit and anti-mouse antibodies, respectively ref 7074 and 7076) were supplied by Cell Signaling Technologies (Danvers, Massachusetts, USA).
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bioRxiv - Cell Biology 2024Quote: Antibodies against pAKT (Ser473) pAKT (Thr308) and AKT (pan) (all rabbit polyclonal) and antibody against β-actin was from Cell Signaling Technology (Danvers, MA, USA). Antibody against fibronectin (ab6328 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualised using the following primary and secondary antibody combinations: Cyclin B1 was detected with a monoclonal rabbit antibody (Cell Signaling Technology, 12231S, 1:800) and visualised using a goat anti-rabbit Alexa Fluor 647 secondary antibody (Molecular Probes ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to immunoblotting with the primary anti-mouse anti-DDDDK-tag antibody (MBL Life science, M185-3L, Japan) or anti-rabbit anti-eIF6 antibody (Cell Signaling Technology, 3833, MA, USA). After incubation with the primary antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed thrice in PBS-T after primary antibody incubation and incubated with secondary antibodies like donkey anti-MouseDyLight 800 (Cell Signaling Technology, cat no#5257) and donkey anti-Rabbit680 (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... The custom mouse anti-BbZIP monoclonal antibody was used to detect BbZIP in Western blots and HRP-conjugated anti-mouse immunoglobulin G antibody was used as the secondary antibody at 1:5000 dilution (Cell Signaling Technology, catalog number 7076S). Images of the blots visualized by chemiluminescence (VWR ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were analyzed by Western blot using the custom mouse anti-BbZIP antibody reported previously38 and an HRP-conjugated anti-mouse immunoglobulin G antibody (Cell Signaling Technology, Catalog# 7076 S) at 1:5000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... we used identical procedures except that we used LV-Syn-DIO-mTagBFP2-2A-GluN2B (1 × 109 TU/ml titer, 2 μL) and GluN2B antibody (#4207S, Cell Signaling, 1:1000, rabbit polyclonal antibody) (Supplementary Fig ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane parts were probed for 18 h with anti-hsTRPM2 antibody from rabbit (Novus #nb500-241 at 1:50000 dilution) and anti-Na+/K+-ATPase antibody from rabbit (Cell Signaling #3010 at 1:1000 dilution), respectively ...
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bioRxiv - Developmental Biology 2023Quote: ... diluted 1:100), Rat anti-GFP monoclonal antibody (nacalai tesque, GF090R, diluted 1:1000) and rabbit anti-pSmad1/5/9 antibody (Cell Signaling Technology, #13820, diluted 1:100). After the primary antibody incubation ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed for 30 min with TBS-0.05% Tween 20 (TBS-T) and primary antibodies were detected using horseradish peroxidase-conjugated goat anti-mouse secondary antibody diluted 1:2000 (Cell Signaling Technology, Inc., Danvers, MA, USA) and incubated at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% milk in 1X TTBS and incubated overnight with the appropriate primary antibody (Cell Signaling c-IAP2 58C7 Rabbit mAb #3130; Cell Signaling XIAP Antibody #2042; Cell Signaling Mcl-1 (D2W9E) Rabbit mAb #94296 ...
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bioRxiv - Cancer Biology 2021Quote: Protein samples were collected and lysed using RIPA buffer containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysates were quantified by Bradford assay (Bio-rad) ...
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bioRxiv - Neuroscience 2020Quote: ... SOD2 and apoptosis-related proteins Cleaved-Caspase 3 were purchased from Cell Signaling Technology (Beverly, MA, USA). After the membranes were washed three times for 10 minutes each in PBST ...
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bioRxiv - Neuroscience 2021Quote: ... Protein expression was normalized to the internal control β-actin (1:100; CST #4970, Cell Signaling Technologies). In order to generate comparable data for all samples ...
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bioRxiv - Cell Biology 2020Quote: ... total proteins were extracted by homogenizing cells in RIPA lysis buffer (cat. no. 9806, Cell Signaling Technology) and centrifuging at 14,000 g for 15 minutes at 4 °C ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: ... Total proteins from these supernatant-derived pellets were isolated using RIPA buffer (Cell Signaling Technology, Danvers, MA) supplemented with 1 mM PMSF ...
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bioRxiv - Microbiology 2022Quote: ... pH 2.2) and restained for total proteins using NFkB p65 (L8F6) mouse mAb (1:1000) (Cell Signaling) or Stat3 (124H6 ...
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bioRxiv - Pathology 2020Quote: Total protein lysate was collected by homogenization of flash frozen liver in RIPA lysis buffer (Cell Signaling). Protein concentrations were determined using the Bradford assay (Bio-Rad ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Depletion of the proteins was confirmed via western blot analysis using anti-SMG1 (Cell Signaling Technology, 9149), and anti-UPF1 (Cell Signaling Technology ...
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bioRxiv - Physiology 2021Quote: Total protein was extracted from cells or tissues using cell lysis buffer (Cell Signaling, Cat No. 9803), containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Neuroscience 2023Quote: ... Rabbit polyclonal phospho-S6 ribosomal protein (S240/244) (1/2000; Cell Signaling, Beverly, MA #5364, RRID:AB 10694233) was incubated 48 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 3-day differentiated SKM cells using cell lysis buffer (9803S, Cell Signaling Technology), supplemented with a complete protease and phosphatase inhibitor cocktail (78442 ...
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bioRxiv - Cell Biology 2022Quote: ... 90 μg of protein was used for western blotting with cleaved caspase-3 (Cell Signaling Technologies, 9661), actin (Sigma ...
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bioRxiv - Physiology 2024Quote: ... Data were normalized for the expression of the housekeeping protein β-actin (cs4967, Cell Signaling, 1:5000).
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bioRxiv - Molecular Biology 2024Quote: Protein extract was isolated from cells using 1x Cell Signaling Lysis Buffer (Cell Signaling, Danvers, MA, USA), supplemented with 1x Halt™ Protease Inhibitor Cocktail EDTA-free (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... anti-Microtubule-associated protein 1 light chain B (anti-MAPLC3/LC3; for western blot: Cell Signaling Technology-CST ...
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bioRxiv - Cell Biology 2023Quote: ... the protein-DNA crosslinks in the immunoprecipitates were uncoupled using ChIP Elution Buffer (7009, Cell Signaling Technology). The DNA was subsequently purified using DNA purification columns ...
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bioRxiv - Molecular Biology 2022Quote: ... and associated proteins were harvested from top layer with RIPA buffer containing phosphatase inhibitor cocktail (Cell Signaling), and protease inhibitor cocktail (Sigma).
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bioRxiv - Genetics 2023Quote: ... Immune complexes were precipitated using either protein A or G agarose beads (Cell Signaling Technology, Danvers, MA) for 4 h at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of pre-cleared protein lysates were bounded with anti-HA magnetic beads (Cell Signaling Technology) or anti-V5 magnetic beads (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... Clarified lysates were pre-cleared with 15 µL washed Protein A magnetic beads (Cell Signaling Technology 73778) for 30 minutes at 4 °C with rotation ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred onto PVDF membranes and detected with rabbit monoclonal anti-DAXX (Cell Signaling clone 25C12).
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bioRxiv - Biophysics 2021Quote: ... Antibodies used include anti-GAPDH (Cell Signaling #2118, 1:1000 dilution), anti-α-tubulin (Cell Signaling #2144 ...
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bioRxiv - Cancer Biology 2021Quote: ... then incubating with horseradish peroxidase conjugated secondary antibody (Cell Signaling Technology), signals were developed using SuperSignal West (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were incubated with Stat3 antibody (1:100, Cell Signaling #9139) for 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies for western blotting were YAP/TAZ (8418, Cell Signaling), ALDH1A1 (54135 ...
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bioRxiv - Cancer Biology 2021Quote: ... a rabbit monoclonal anti-AKT antibody (1:500; Cell Signaling Technology), a rabbit monoclonal anti-p-mTOR (1:500 ...